Quantification and Identification of Culturable Airborne Bacteria from Duck Houses
Identifieur interne : 000173 ( Main/Corpus ); précédent : 000172; suivant : 000174Quantification and Identification of Culturable Airborne Bacteria from Duck Houses
Auteurs : Elena Martin ; Peter Kmpfer ; Udo JckelSource :
- Annals of Occupational Hygiene [ 0003-4878 ] ; 2009-12-30.
Abstract
Employees at agricultural working places are often exposed to complex bioaerosols. Investigations of bioaerosols in duck houses revealed concentrations of cultivable bacteria between 0.4 and 3105 colony forming units (CFU) m3 on tryptone soy agar, 0.3 and 2105 CFU m3 on actinomycetes isolation agar, and 0.8 and 5103 CFU m3 on Middlebrook agar, respectively, when incubated at 25C. At an incubation temperature of 37C, 0.63102 CFU m3 were counted on MacConkey agar and 0.32103 CFU m3 on Middlebrook agar, and the concentrations of bacteria on glycerolarginine agar and oatmeal agar incubated at 50C varied between 0.1 and 2103 and 1 and 7103 CFU m3, respectively. In addition, high concentrations of cells were observed by fluorescence microscope quantification of cell counts after 4,6-diamidino-2-phenylindol staining with 38107 cells m3. A total of 213 colonies with different morphological appearance were selected and the isolated pure cultures were identified at the genus level using the 16S rRNA gene sequence analyses. In summary, 19 different genera of Actinobacteria, four genera of the Firmicutes, one genus of the Bacteroidetes, and five genera of the Proteobacteria were identified. Several isolates represent new phylogenetic lineages. Based on 16S rRNA gene analyses, some isolates were most closely related to Cellulosimicrobium funkei, Corynebacterium falsenii, Corynebacterium xerosis, Mycobacterium arupense, and Staphylococcus epidermidis, which have been grouped into Risk group 2 of biological agents and may cause negative pulmonary health effects. These bacterial species were present in high concentrations up to 104 CFU m3. For this reason, we recommend an adequate personal breathing protection at these working places.
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DOI: 10.1093/annhyg/mep088
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Investigations of bioaerosols in duck houses revealed concentrations of cultivable bacteria between 0.4 and 3105 colony forming units (CFU) m3 on tryptone soy agar, 0.3 and 2105 CFU m3 on actinomycetes isolation agar, and 0.8 and 5103 CFU m3 on Middlebrook agar, respectively, when incubated at 25C. At an incubation temperature of 37C, 0.63102 CFU m3 were counted on MacConkey agar and 0.32103 CFU m3 on Middlebrook agar, and the concentrations of bacteria on glycerolarginine agar and oatmeal agar incubated at 50C varied between 0.1 and 2103 and 1 and 7103 CFU m3, respectively. In addition, high concentrations of cells were observed by fluorescence microscope quantification of cell counts after 4,6-diamidino-2-phenylindol staining with 38107 cells m3. A total of 213 colonies with different morphological appearance were selected and the isolated pure cultures were identified at the genus level using the 16S rRNA gene sequence analyses. In summary, 19 different genera of Actinobacteria, four genera of the Firmicutes, one genus of the Bacteroidetes, and five genera of the Proteobacteria were identified. Several isolates represent new phylogenetic lineages. Based on 16S rRNA gene analyses, some isolates were most closely related to Cellulosimicrobium funkei, Corynebacterium falsenii, Corynebacterium xerosis, Mycobacterium arupense, and Staphylococcus epidermidis, which have been grouped into Risk group 2 of biological agents and may cause negative pulmonary health effects. These bacterial species were present in high concentrations up to 104 CFU m3. For this reason, we recommend an adequate personal breathing protection at these working places.</div></front></TEI><istex><corpusName>oup</corpusName><author><json:item><name>Elena Martin</name><affiliations><json:string>Federal Institute for Occupational Safety and Health, Nldnerstrasse 40-42, 10317 Berlin, Germany</json:string></affiliations></json:item><json:item><name>Peter Kmpfer</name><affiliations><json:string>Institute for Applied Microbiology, Justus-Liebig-University Giessen, Heinrich- Buff-Ring 26-32, 35392 Giessen, Germany</json:string></affiliations></json:item><json:item><name>Udo Jckel</name><affiliations><json:string>Federal Institute for Occupational Safety and Health, Nldnerstrasse 40-42, 10317 Berlin, Germany</json:string><json:string>E-mail: jaeckel.udo@baua.bund.de</json:string></affiliations></json:item></author><subject><json:item><lang><json:string>eng</json:string></lang><value>Original Articles</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>airborne bacteria</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>bioaerosol</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>duck house</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>identification</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>PCR</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>16S rRNA gene</value></json:item></subject><language><json:string>eng</json:string></language><abstract>Employees at agricultural working places are often exposed to complex bioaerosols. Investigations of bioaerosols in duck houses revealed concentrations of cultivable bacteria between 0.4 and 3105 colony forming units (CFU) m3 on tryptone soy agar, 0.3 and 2105 CFU m3 on actinomycetes isolation agar, and 0.8 and 5103 CFU m3 on Middlebrook agar, respectively, when incubated at 25C. At an incubation temperature of 37C, 0.63102 CFU m3 were counted on MacConkey agar and 0.32103 CFU m3 on Middlebrook agar, and the concentrations of bacteria on glycerolarginine agar and oatmeal agar incubated at 50C varied between 0.1 and 2103 and 1 and 7103 CFU m3, respectively. In addition, high concentrations of cells were observed by fluorescence microscope quantification of cell counts after 4,6-diamidino-2-phenylindol staining with 38107 cells m3. A total of 213 colonies with different morphological appearance were selected and the isolated pure cultures were identified at the genus level using the 16S rRNA gene sequence analyses. In summary, 19 different genera of Actinobacteria, four genera of the Firmicutes, one genus of the Bacteroidetes, and five genera of the Proteobacteria were identified. Several isolates represent new phylogenetic lineages. Based on 16S rRNA gene analyses, some isolates were most closely related to Cellulosimicrobium funkei, Corynebacterium falsenii, Corynebacterium xerosis, Mycobacterium arupense, and Staphylococcus epidermidis, which have been grouped into Risk group 2 of biological agents and may cause negative pulmonary health effects. These bacterial species were present in high concentrations up to 104 CFU m3. 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Investigations of bioaerosols in duck houses revealed concentrations of cultivable bacteria between 0.4 and 3105 colony forming units (CFU) m3 on tryptone soy agar, 0.3 and 2105 CFU m3 on actinomycetes isolation agar, and 0.8 and 5103 CFU m3 on Middlebrook agar, respectively, when incubated at 25C. At an incubation temperature of 37C, 0.63102 CFU m3 were counted on MacConkey agar and 0.32103 CFU m3 on Middlebrook agar, and the concentrations of bacteria on glycerolarginine agar and oatmeal agar incubated at 50C varied between 0.1 and 2103 and 1 and 7103 CFU m3, respectively. In addition, high concentrations of cells were observed by fluorescence microscope quantification of cell counts after 4,6-diamidino-2-phenylindol staining with 38107 cells m3. A total of 213 colonies with different morphological appearance were selected and the isolated pure cultures were identified at the genus level using the 16S rRNA gene sequence analyses. In summary, 19 different genera of Actinobacteria, four genera of the Firmicutes, one genus of the Bacteroidetes, and five genera of the Proteobacteria were identified. Several isolates represent new phylogenetic lineages. Based on 16S rRNA gene analyses, some isolates were most closely related to Cellulosimicrobium funkei, Corynebacterium falsenii, Corynebacterium xerosis, Mycobacterium arupense, and Staphylococcus epidermidis, which have been grouped into Risk group 2 of biological agents and may cause negative pulmonary health effects. These bacterial species were present in high concentrations up to 104 CFU m3. For this reason, we recommend an adequate personal breathing protection at these working places.</p></abstract><textClass><keywords scheme="keyword"><list><item><term>Original Articles</term></item></list></keywords></textClass><textClass><keywords scheme="keyword"><list><head>Keywords</head><item><term>airborne bacteria</term></item><item><term>bioaerosol</term></item><item><term>duck house</term></item><item><term>identification</term></item><item><term>PCR</term></item><item><term>16S rRNA gene</term></item></list></keywords></textClass></profileDesc><revisionDesc><change when="2009-12-30">Created</change><change when="2009-12-30">Published</change><change xml:id="refBibs-istex" who="#ISTEX-API" when="2016-0-26">References added</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><original>false</original><mimetype>text/plain</mimetype><extension>txt</extension><uri>https://api.istex.fr/document/536DEBD0BF11F7E6ABD87A1134DBA67D78B22D27/fulltext/txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="corpus oup" wicri:toSee="no header"><istex:docType PUBLIC="-//NLM//DTD Journal Publishing DTD v2.3 20070202//EN" URI="journalpublishing.dtd" name="istex:docType"></istex:docType><istex:document><article article-type="research-article"><front><journal-meta><journal-id journal-id-type="hwp">annhyg</journal-id><journal-id journal-id-type="publisher-id">annhyg</journal-id><journal-title>Annals of Occupational Hygiene</journal-title><issn pub-type="epub">1475-3162</issn><issn pub-type="ppub">0003-4878</issn><publisher><publisher-name>Oxford University Press</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="doi">10.1093/annhyg/mep088</article-id><article-categories><subj-group><subject>Original Articles</subject></subj-group></article-categories><title-group><article-title>Quantification and Identification of Culturable Airborne Bacteria from Duck Houses</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Martin</surname><given-names>Elena</given-names></name><xref ref-type="aff" rid="aff1">1</xref></contrib><contrib contrib-type="author"><name><surname>Kämpfer</surname><given-names>Peter</given-names></name><xref ref-type="aff" rid="aff2">2</xref></contrib><contrib contrib-type="author" corresp="yes"><name><surname>Jäckel</surname><given-names>Udo</given-names></name><xref ref-type="aff" rid="aff1">1</xref><xref ref-type="corresp" rid="cor1">*</xref></contrib></contrib-group><aff id="aff1"><label>1</label>Federal Institute for Occupational Safety and Health, Nöldnerstrasse 40-42, 10317 Berlin, Germany</aff><aff id="aff2"><label>2</label>Institute for Applied Microbiology, Justus-Liebig-University Giessen, Heinrich- Buff-Ring 26-32, 35392 Giessen, Germany</aff><author-notes><corresp id="cor1"><label>*</label>Author to whom correspondence should be addressed. Tel: +0049-30-515-48-4788; fax: +0049-30-515-48-4171; e-mail: <email>jaeckel.udo@baua.bund.de</email></corresp></author-notes><pub-date pub-type="epub-ppub"><month>3</month><year>2010</year></pub-date><pub-date pub-type="epub"><day>30</day><month>12</month><year>2009</year></pub-date><volume>54</volume><issue>2</issue><fpage>217</fpage><lpage>227</lpage><history><date date-type="received"><day>6</day><month>3</month><year>2009</year></date><date date-type="accepted"><day>29</day><month>9</month><year>2009</year></date></history><permissions><copyright-statement>© The Author 2009. Published by Oxford University Press on behalf of the British Occupational Hygiene Society</copyright-statement><copyright-year>2010</copyright-year></permissions><abstract><p>Employees at agricultural working places are often exposed to complex bioaerosols. Investigations of bioaerosols in duck houses revealed concentrations of cultivable bacteria between 0.4 and 3 × 10<sup>5</sup> colony forming units (CFU) m<sup>−3</sup> on tryptone soy agar, 0.3 and 2 × 10<sup>5</sup> CFU m<sup>−3</sup> on actinomycetes isolation agar, and 0.8 and 5 × 10<sup>3</sup> CFU m<sup>−3</sup> on Middlebrook agar, respectively, when incubated at 25°C. At an incubation temperature of 37°C, 0.6–3 × 10<sup>2</sup> CFU m<sup>−3</sup> were counted on MacConkey agar and 0.3–2 × 10<sup>3</sup> CFU m<sup>−3</sup> on Middlebrook agar, and the concentrations of bacteria on glycerol–arginine agar and oatmeal agar incubated at 50°C varied between 0.1 and 2 × 10<sup>3</sup> and 1 and 7 × 10<sup>3</sup> CFU m<sup>−3</sup>, respectively. In addition, high concentrations of cells were observed by fluorescence microscope quantification of cell counts after 4′,6-diamidino-2-phenylindol staining with 3–8 × 10<sup>7</sup> cells m<sup>−3</sup>. A total of 213 colonies with different morphological appearance were selected and the isolated pure cultures were identified at the genus level using the 16S rRNA gene sequence analyses. In summary, 19 different genera of Actinobacteria, four genera of the Firmicutes, one genus of the Bacteroidetes, and five genera of the Proteobacteria were identified. Several isolates represent new phylogenetic lineages. Based on 16S rRNA gene analyses, some isolates were most closely related to <italic>Cellulosimicrobium funkei</italic>, <italic>Corynebacterium falsenii</italic>, <italic>Corynebacterium xerosis</italic>, <italic>Mycobacterium arupense</italic>, and <italic>Staphylococcus epidermidis</italic>, which have been grouped into Risk group 2 of biological agents and may cause negative pulmonary health effects. These bacterial species were present in high concentrations up to 10<sup>4</sup> CFU m<sup>−3</sup>. For this reason, we recommend an adequate personal breathing protection at these working places.</p></abstract><kwd-group><kwd>airborne bacteria</kwd><kwd>bioaerosol</kwd><kwd>duck house</kwd><kwd>identification</kwd><kwd>PCR</kwd><kwd>16S rRNA gene</kwd></kwd-group></article-meta></front></article></istex:document></istex:metadataXml><mods version="3.6"><titleInfo><title>Quantification and Identification of Culturable Airborne Bacteria from Duck Houses</title></titleInfo><titleInfo type="alternative" contentType="CDATA"><title>Quantification and Identification of Culturable Airborne Bacteria from Duck Houses</title></titleInfo><name type="personal"><namePart type="given">Elena</namePart><namePart type="family">Martin</namePart><affiliation>Federal Institute for Occupational Safety and Health, Nldnerstrasse 40-42, 10317 Berlin, Germany</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Peter</namePart><namePart type="family">Kmpfer</namePart><affiliation>Institute for Applied Microbiology, Justus-Liebig-University Giessen, Heinrich- Buff-Ring 26-32, 35392 Giessen, Germany</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal" displayLabel="corresp"><namePart type="given">Udo</namePart><namePart type="family">Jckel</namePart><affiliation>Federal Institute for Occupational Safety and Health, Nldnerstrasse 40-42, 10317 Berlin, Germany</affiliation><affiliation>E-mail: jaeckel.udo@baua.bund.de</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article">research-article</genre><subject><topic>Original Articles</topic></subject><originInfo><publisher>Oxford University Press</publisher><dateIssued encoding="w3cdtf">2009-12-30</dateIssued><dateCreated encoding="w3cdtf">2009-12-30</dateCreated><copyrightDate encoding="w3cdtf">2010</copyrightDate></originInfo><language><languageTerm type="code" authority="iso639-2b">eng</languageTerm><languageTerm type="code" authority="rfc3066">en</languageTerm></language><physicalDescription><internetMediaType>text/html</internetMediaType></physicalDescription><abstract>Employees at agricultural working places are often exposed to complex bioaerosols. Investigations of bioaerosols in duck houses revealed concentrations of cultivable bacteria between 0.4 and 3105 colony forming units (CFU) m3 on tryptone soy agar, 0.3 and 2105 CFU m3 on actinomycetes isolation agar, and 0.8 and 5103 CFU m3 on Middlebrook agar, respectively, when incubated at 25C. At an incubation temperature of 37C, 0.63102 CFU m3 were counted on MacConkey agar and 0.32103 CFU m3 on Middlebrook agar, and the concentrations of bacteria on glycerolarginine agar and oatmeal agar incubated at 50C varied between 0.1 and 2103 and 1 and 7103 CFU m3, respectively. In addition, high concentrations of cells were observed by fluorescence microscope quantification of cell counts after 4,6-diamidino-2-phenylindol staining with 38107 cells m3. A total of 213 colonies with different morphological appearance were selected and the isolated pure cultures were identified at the genus level using the 16S rRNA gene sequence analyses. In summary, 19 different genera of Actinobacteria, four genera of the Firmicutes, one genus of the Bacteroidetes, and five genera of the Proteobacteria were identified. Several isolates represent new phylogenetic lineages. Based on 16S rRNA gene analyses, some isolates were most closely related to Cellulosimicrobium funkei, Corynebacterium falsenii, Corynebacterium xerosis, Mycobacterium arupense, and Staphylococcus epidermidis, which have been grouped into Risk group 2 of biological agents and may cause negative pulmonary health effects. These bacterial species were present in high concentrations up to 104 CFU m3. For this reason, we recommend an adequate personal breathing protection at these working places.</abstract><subject><genre>Keywords</genre><topic>airborne bacteria</topic><topic>bioaerosol</topic><topic>duck house</topic><topic>identification</topic><topic>PCR</topic><topic>16S rRNA gene</topic></subject><relatedItem type="host"><titleInfo><title>Annals of Occupational Hygiene</title></titleInfo><identifier type="ISSN">0003-4878</identifier><identifier type="eISSN">1475-3162</identifier><identifier type="JournalID">annhyg</identifier><identifier type="JournalID-hwp">annhyg</identifier><part><date>2009</date><detail type="volume"><caption>vol.</caption><number>54</number></detail><detail type="issue"><caption>no.</caption><number>2</number></detail><extent unit="pages"><start>217</start><end>227</end></extent></part></relatedItem><identifier type="istex">536DEBD0BF11F7E6ABD87A1134DBA67D78B22D27</identifier><identifier type="DOI">10.1093/annhyg/mep088</identifier><accessCondition type="use and reproduction" contentType="Copyright">The Author 2009. 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