Development of a piezoelectric immunosensor for the measurement of paclitaxel.
Identifieur interne : 000487 ( PubMed/Curation ); précédent : 000486; suivant : 000488Development of a piezoelectric immunosensor for the measurement of paclitaxel.
Auteurs : L. Pastorino [Italie] ; F. Caneva Soumetz ; M. Giacomini ; C. RuggieroSource :
- Journal of immunological methods [ 0022-1759 ] ; 2006.
English descriptors
- KwdEn :
- Animals, Antibodies, Monoclonal (chemistry), Antibodies, Monoclonal (immunology), Biosensing Techniques (methods), Calibration, Cattle, Microscopy, Atomic Force, Paclitaxel (analysis), Paclitaxel (immunology), Polyethylenes (chemistry), Polystyrenes (chemistry), Quartz (chemistry), Quaternary Ammonium Compounds (chemistry), Serum Albumin, Bovine (chemistry), Surface Properties, Taxoids (immunology).
- MESH :
- chemical , analysis : Paclitaxel.
- chemical , chemistry : Antibodies, Monoclonal, Polyethylenes, Polystyrenes, Quartz, Quaternary Ammonium Compounds, Serum Albumin, Bovine.
- chemical , immunology : Antibodies, Monoclonal, Paclitaxel, Taxoids.
- methods : Biosensing Techniques.
- Animals, Calibration, Cattle, Microscopy, Atomic Force, Surface Properties.
Abstract
This paper describes the development of a piezoelectric immunosensor for the measurement of paclitaxel (taxol), a natural anti-cancer agent. An antibody specific for taxanes was immobilized onto the surface of quartz crystals by means of the layer-by-layer self-assembly technique. The immobilization was achieved using electrostatic interactions between a precursor layer and the antibody molecules. The assembly process was monitored by a quartz crystal microbalance (QCM) and the topography of the modified quartz crystals was investigated by means of atomic force microscopy. The specific interaction of the immobilized antibody with paclitaxel in solution at different concentrations was monitored as a change in resonant frequency of the modified crystal. Moreover, the influence of non-specific adsorption was also characterized. The results show that the proposed immunosensor offers a promising alternative to classical analytical methods for a fast and easy determination of paclitaxel.
DOI: 10.1016/j.jim.2006.05.003
PubMed: 16774765
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: Pour aller vers cette notice dans l'étape Curation :000487
Links to Exploration step
pubmed:16774765Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Development of a piezoelectric immunosensor for the measurement of paclitaxel.</title><author><name sortKey="Pastorino, L" sort="Pastorino, L" uniqKey="Pastorino L" first="L" last="Pastorino">L. Pastorino</name><affiliation wicri:level="1"><nlm:affiliation>Department of Communication, Computer and System Sciences, University of Genoa, Via Opera Pia, 13-16145 Genova, Italy.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>Department of Communication, Computer and System Sciences, University of Genoa, Via Opera Pia, 13-16145 Genova</wicri:regionArea></affiliation></author><author><name sortKey="Caneva Soumetz, F" sort="Caneva Soumetz, F" uniqKey="Caneva Soumetz F" first="F" last="Caneva Soumetz">F. Caneva Soumetz</name></author><author><name sortKey="Giacomini, M" sort="Giacomini, M" uniqKey="Giacomini M" first="M" last="Giacomini">M. Giacomini</name></author><author><name sortKey="Ruggiero, C" sort="Ruggiero, C" uniqKey="Ruggiero C" first="C" last="Ruggiero">C. Ruggiero</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2006">2006</date><idno type="doi">10.1016/j.jim.2006.05.003</idno><idno type="RBID">pubmed:16774765</idno><idno type="pmid">16774765</idno><idno type="wicri:Area/PubMed/Corpus">000487</idno><idno type="wicri:Area/PubMed/Curation">000487</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Development of a piezoelectric immunosensor for the measurement of paclitaxel.</title><author><name sortKey="Pastorino, L" sort="Pastorino, L" uniqKey="Pastorino L" first="L" last="Pastorino">L. Pastorino</name><affiliation wicri:level="1"><nlm:affiliation>Department of Communication, Computer and System Sciences, University of Genoa, Via Opera Pia, 13-16145 Genova, Italy.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>Department of Communication, Computer and System Sciences, University of Genoa, Via Opera Pia, 13-16145 Genova</wicri:regionArea></affiliation></author><author><name sortKey="Caneva Soumetz, F" sort="Caneva Soumetz, F" uniqKey="Caneva Soumetz F" first="F" last="Caneva Soumetz">F. Caneva Soumetz</name></author><author><name sortKey="Giacomini, M" sort="Giacomini, M" uniqKey="Giacomini M" first="M" last="Giacomini">M. Giacomini</name></author><author><name sortKey="Ruggiero, C" sort="Ruggiero, C" uniqKey="Ruggiero C" first="C" last="Ruggiero">C. Ruggiero</name></author></analytic><series><title level="j">Journal of immunological methods</title><idno type="ISSN">0022-1759</idno><imprint><date when="2006" type="published">2006</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Antibodies, Monoclonal (chemistry)</term><term>Antibodies, Monoclonal (immunology)</term><term>Biosensing Techniques (methods)</term><term>Calibration</term><term>Cattle</term><term>Microscopy, Atomic Force</term><term>Paclitaxel (analysis)</term><term>Paclitaxel (immunology)</term><term>Polyethylenes (chemistry)</term><term>Polystyrenes (chemistry)</term><term>Quartz (chemistry)</term><term>Quaternary Ammonium Compounds (chemistry)</term><term>Serum Albumin, Bovine (chemistry)</term><term>Surface Properties</term><term>Taxoids (immunology)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Paclitaxel</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Antibodies, Monoclonal</term><term>Polyethylenes</term><term>Polystyrenes</term><term>Quartz</term><term>Quaternary Ammonium Compounds</term><term>Serum Albumin, Bovine</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Antibodies, Monoclonal</term><term>Paclitaxel</term><term>Taxoids</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Biosensing Techniques</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Calibration</term><term>Cattle</term><term>Microscopy, Atomic Force</term><term>Surface Properties</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">This paper describes the development of a piezoelectric immunosensor for the measurement of paclitaxel (taxol), a natural anti-cancer agent. An antibody specific for taxanes was immobilized onto the surface of quartz crystals by means of the layer-by-layer self-assembly technique. The immobilization was achieved using electrostatic interactions between a precursor layer and the antibody molecules. The assembly process was monitored by a quartz crystal microbalance (QCM) and the topography of the modified quartz crystals was investigated by means of atomic force microscopy. The specific interaction of the immobilized antibody with paclitaxel in solution at different concentrations was monitored as a change in resonant frequency of the modified crystal. Moreover, the influence of non-specific adsorption was also characterized. The results show that the proposed immunosensor offers a promising alternative to classical analytical methods for a fast and easy determination of paclitaxel.</div></front></TEI><pubmed><MedlineCitation Owner="NLM" Status="MEDLINE"><PMID Version="1">16774765</PMID><DateCreated><Year>2006</Year><Month>07</Month><Day>14</Day></DateCreated><DateCompleted><Year>2006</Year><Month>09</Month><Day>11</Day></DateCompleted><DateRevised><Year>2015</Year><Month>11</Month><Day>19</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Print">0022-1759</ISSN><JournalIssue CitedMedium="Print"><Volume>313</Volume><Issue>1-2</Issue><PubDate><Year>2006</Year><Month>Jun</Month><Day>30</Day></PubDate></JournalIssue><Title>Journal of immunological methods</Title><ISOAbbreviation>J. Immunol. Methods</ISOAbbreviation></Journal><ArticleTitle>Development of a piezoelectric immunosensor for the measurement of paclitaxel.</ArticleTitle><Pagination><MedlinePgn>191-8</MedlinePgn></Pagination><Abstract><AbstractText>This paper describes the development of a piezoelectric immunosensor for the measurement of paclitaxel (taxol), a natural anti-cancer agent. An antibody specific for taxanes was immobilized onto the surface of quartz crystals by means of the layer-by-layer self-assembly technique. The immobilization was achieved using electrostatic interactions between a precursor layer and the antibody molecules. The assembly process was monitored by a quartz crystal microbalance (QCM) and the topography of the modified quartz crystals was investigated by means of atomic force microscopy. The specific interaction of the immobilized antibody with paclitaxel in solution at different concentrations was monitored as a change in resonant frequency of the modified crystal. Moreover, the influence of non-specific adsorption was also characterized. The results show that the proposed immunosensor offers a promising alternative to classical analytical methods for a fast and easy determination of paclitaxel.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Pastorino</LastName><ForeName>L</ForeName><Initials>L</Initials><AffiliationInfo><Affiliation>Department of Communication, Computer and System Sciences, University of Genoa, Via Opera Pia, 13-16145 Genova, Italy.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Caneva Soumetz</LastName><ForeName>F</ForeName><Initials>F</Initials></Author><Author ValidYN="Y"><LastName>Giacomini</LastName><ForeName>M</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>Ruggiero</LastName><ForeName>C</ForeName><Initials>C</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2006</Year><Month>06</Month><Day>08</Day></ArticleDate></Article><MedlineJournalInfo><Country>Netherlands</Country><MedlineTA>J Immunol Methods</MedlineTA><NlmUniqueID>1305440</NlmUniqueID><ISSNLinking>0022-1759</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000911">Antibodies, Monoclonal</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D011095">Polyethylenes</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D011137">Polystyrenes</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000644">Quaternary Ammonium Compounds</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D012710">Serum Albumin, Bovine</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D043823">Taxoids</NameOfSubstance></Chemical><Chemical><RegistryNumber>14808-60-7</RegistryNumber><NameOfSubstance UI="D011791">Quartz</NameOfSubstance></Chemical><Chemical><RegistryNumber>26062-79-3</RegistryNumber><NameOfSubstance UI="C041004">poly-N,N-dimethyl-N,N-diallylammonium chloride</NameOfSubstance></Chemical><Chemical><RegistryNumber>70KO0R01RY</RegistryNumber><NameOfSubstance UI="C003321">polystyrene sulfonic acid</NameOfSubstance></Chemical><Chemical><RegistryNumber>P88XT4IS4D</RegistryNumber><NameOfSubstance UI="D017239">Paclitaxel</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName MajorTopicYN="N" UI="D000818">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N" UI="D000911">Antibodies, Monoclonal</DescriptorName><QualifierName MajorTopicYN="N" UI="Q000737">chemistry</QualifierName><QualifierName MajorTopicYN="N" UI="Q000276">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N" UI="D015374">Biosensing Techniques</DescriptorName><QualifierName MajorTopicYN="Y" UI="Q000379">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N" UI="D002138">Calibration</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N" UI="D002417">Cattle</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N" UI="D018625">Microscopy, Atomic Force</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N" UI="D017239">Paclitaxel</DescriptorName><QualifierName MajorTopicYN="Y" UI="Q000032">analysis</QualifierName><QualifierName MajorTopicYN="N" UI="Q000276">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N" UI="D011095">Polyethylenes</DescriptorName><QualifierName MajorTopicYN="N" UI="Q000737">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N" UI="D011137">Polystyrenes</DescriptorName><QualifierName MajorTopicYN="N" UI="Q000737">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N" UI="D011791">Quartz</DescriptorName><QualifierName MajorTopicYN="N" UI="Q000737">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N" UI="D000644">Quaternary Ammonium Compounds</DescriptorName><QualifierName MajorTopicYN="N" UI="Q000737">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N" UI="D012710">Serum Albumin, Bovine</DescriptorName><QualifierName MajorTopicYN="N" UI="Q000737">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N" UI="D013499">Surface Properties</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N" UI="D043823">Taxoids</DescriptorName><QualifierName MajorTopicYN="N" UI="Q000276">immunology</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2006</Year><Month>2</Month><Day>20</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2006</Year><Month>4</Month><Day>26</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2006</Year><Month>5</Month><Day>10</Day></PubMedPubDate><PubMedPubDate PubStatus="aheadofprint"><Year>2006</Year><Month>6</Month><Day>8</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2006</Year><Month>6</Month><Day>16</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2006</Year><Month>9</Month><Day>12</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2006</Year><Month>6</Month><Day>16</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pii">S0022-1759(06)00133-5</ArticleId><ArticleId IdType="doi">10.1016/j.jim.2006.05.003</ArticleId><ArticleId IdType="pubmed">16774765</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Musique/explor/OperaV1/Data/PubMed/Curation
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000487 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/PubMed/Curation/biblio.hfd -nk 000487 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Musique
|area= OperaV1
|flux= PubMed
|étape= Curation
|type= RBID
|clé= pubmed:16774765
|texte= Development of a piezoelectric immunosensor for the measurement of paclitaxel.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/PubMed/Curation/RBID.i -Sk "pubmed:16774765" \
| HfdSelect -Kh $EXPLOR_AREA/Data/PubMed/Curation/biblio.hfd \
| NlmPubMed2Wicri -a OperaV1
| This area was generated with Dilib version V0.6.21. |  |