Preparation and characterization of a microencapsulated polyethylene glycol cross-linked polyhemoglobin.
Identifieur interne : 000060 ( PubMed/Corpus ); précédent : 000059; suivant : 000061Preparation and characterization of a microencapsulated polyethylene glycol cross-linked polyhemoglobin.
Auteurs : Marcos C. Knirsch ; Filippo Dell'Anno ; Dennis Chicoma ; Marco Antonio Stephano ; Nádia A. Bou-Chacra ; Domenico Palombo ; Attilio Converti ; Bronislaw PolakiewiczSource :
- Bioprocess and biosystems engineering ; 2015.
Abstract
Many complications are associated to the therapeutic use of blood, among which are not only transfusion adverse events but also other issues such as lack of donors and high costs for collecting, testing, preserving, and distributing blood packages. Therefore, a clinically viable "blood substitute" is considered the holy grail of traumatology and may greatly benefit medicine. One of the most successful approaches to date is conjugating hemoglobin with polyethylene glycol (PEG). This conjugation aims mainly at overcoming free cell hemoglobin toxicity, which makes its use as oxygen carrier in pure form unfeasible. To improve PEG-hemoglobin conjugates feasibility, we propose applying dual functional PEG cross-linking hemoglobin molecules encapsulated by a protein carrier. The new oxygen carrier showed mean values of the hydrodynamic diameter, dispersity, and zeta potential of 1370 nm, 0.029 and -36 mV, respectively, evidencing the successful synthesis of PEG bis(N-succinimidyl succinate) and polyhemoglobin as well as the structuring of protein carrier.
DOI: 10.1007/s00449-015-1463-y
PubMed: 26314399
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Prof. Lineu Prestes, 580 B16, Cidade Universitária, São Paulo, SP, 05508-000, Brazil.</nlm:affiliation></affiliation></author><author><name sortKey="Dell Anno, Filippo" sort="Dell Anno, Filippo" uniqKey="Dell Anno F" first="Filippo" last="Dell'Anno">Filippo Dell'Anno</name><affiliation><nlm:affiliation>Department of Civil, Chemical and Environmental Engineering, Pole of Chemical Engineering, Polytechnic School, Genoa University, Via Opera Pia 15, 16145, Genoa, Italy.</nlm:affiliation></affiliation></author><author><name sortKey="Chicoma, Dennis" sort="Chicoma, Dennis" uniqKey="Chicoma D" first="Dennis" last="Chicoma">Dennis Chicoma</name><affiliation><nlm:affiliation>Chemical Engineering Department, Polytechnic School, University of São Paulo, Av. Prof. Lineu Prestes 580 B18, Cidade Universitária, São Paulo, SP, 05508-000, Brazil.</nlm:affiliation></affiliation></author><author><name sortKey="Stephano, Marco Antonio" sort="Stephano, Marco Antonio" uniqKey="Stephano M" first="Marco Antonio" last="Stephano">Marco Antonio Stephano</name><affiliation><nlm:affiliation>Department of Biochemical and Pharmaceutical Technology, Faculty of Pharmaceutical Sciences, University of São Paulo, Av. Prof. Lineu Prestes, 580 B16, Cidade Universitária, São Paulo, SP, 05508-000, Brazil.</nlm:affiliation></affiliation></author><author><name sortKey="Bou Chacra, Nadia A" sort="Bou Chacra, Nadia A" uniqKey="Bou Chacra N" first="Nádia A" last="Bou-Chacra">Nádia A. Bou-Chacra</name><affiliation><nlm:affiliation>Department of Pharmacy, Faculty of Pharmaceutical Sciences, University of São Paulo, Av. Prof. Lineu Prestes 580 B15, Cidade Universitária, São Paulo, SP, 05508-000, Brazil.</nlm:affiliation></affiliation></author><author><name sortKey="Palombo, Domenico" sort="Palombo, Domenico" uniqKey="Palombo D" first="Domenico" last="Palombo">Domenico Palombo</name><affiliation><nlm:affiliation>Vascular and Endovascular Surgery Unit, Research Laboratory of Experimental and Clinical Vascular Biology, DISC, Genoa University and IRCCS San Martino Hospital, Largo Rosanna Benzi, 16132, Genoa, Italy.</nlm:affiliation></affiliation></author><author><name sortKey="Converti, Attilio" sort="Converti, Attilio" uniqKey="Converti A" first="Attilio" last="Converti">Attilio Converti</name><affiliation><nlm:affiliation>Department of Civil, Chemical and Environmental Engineering, Pole of Chemical Engineering, Polytechnic School, Genoa University, Via Opera Pia 15, 16145, Genoa, Italy. converti@unige.it.</nlm:affiliation></affiliation></author><author><name sortKey="Polakiewicz, Bronislaw" sort="Polakiewicz, Bronislaw" uniqKey="Polakiewicz B" first="Bronislaw" last="Polakiewicz">Bronislaw Polakiewicz</name><affiliation><nlm:affiliation>Department of Biochemical and Pharmaceutical Technology, Faculty of Pharmaceutical Sciences, University of São Paulo, Av. Prof. Lineu Prestes, 580 B16, Cidade Universitária, São Paulo, SP, 05508-000, Brazil.</nlm:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2015">2015</date><idno type="RBID">pubmed:26314399</idno><idno type="pmid">26314399</idno><idno type="doi">10.1007/s00449-015-1463-y</idno><idno type="wicri:Area/PubMed/Corpus">000060</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Preparation and characterization of a microencapsulated polyethylene glycol cross-linked polyhemoglobin.</title><author><name sortKey="Knirsch, Marcos C" sort="Knirsch, Marcos C" uniqKey="Knirsch M" first="Marcos C" last="Knirsch">Marcos C. Knirsch</name><affiliation><nlm:affiliation>Department of Biochemical and Pharmaceutical Technology, Faculty of Pharmaceutical Sciences, University of São Paulo, Av. Prof. Lineu Prestes, 580 B16, Cidade Universitária, São Paulo, SP, 05508-000, Brazil.</nlm:affiliation></affiliation></author><author><name sortKey="Dell Anno, Filippo" sort="Dell Anno, Filippo" uniqKey="Dell Anno F" first="Filippo" last="Dell'Anno">Filippo Dell'Anno</name><affiliation><nlm:affiliation>Department of Civil, Chemical and Environmental Engineering, Pole of Chemical Engineering, Polytechnic School, Genoa University, Via Opera Pia 15, 16145, Genoa, Italy.</nlm:affiliation></affiliation></author><author><name sortKey="Chicoma, Dennis" sort="Chicoma, Dennis" uniqKey="Chicoma D" first="Dennis" last="Chicoma">Dennis Chicoma</name><affiliation><nlm:affiliation>Chemical Engineering Department, Polytechnic School, University of São Paulo, Av. Prof. Lineu Prestes 580 B18, Cidade Universitária, São Paulo, SP, 05508-000, Brazil.</nlm:affiliation></affiliation></author><author><name sortKey="Stephano, Marco Antonio" sort="Stephano, Marco Antonio" uniqKey="Stephano M" first="Marco Antonio" last="Stephano">Marco Antonio Stephano</name><affiliation><nlm:affiliation>Department of Biochemical and Pharmaceutical Technology, Faculty of Pharmaceutical Sciences, University of São Paulo, Av. Prof. Lineu Prestes, 580 B16, Cidade Universitária, São Paulo, SP, 05508-000, Brazil.</nlm:affiliation></affiliation></author><author><name sortKey="Bou Chacra, Nadia A" sort="Bou Chacra, Nadia A" uniqKey="Bou Chacra N" first="Nádia A" last="Bou-Chacra">Nádia A. Bou-Chacra</name><affiliation><nlm:affiliation>Department of Pharmacy, Faculty of Pharmaceutical Sciences, University of São Paulo, Av. Prof. Lineu Prestes 580 B15, Cidade Universitária, São Paulo, SP, 05508-000, Brazil.</nlm:affiliation></affiliation></author><author><name sortKey="Palombo, Domenico" sort="Palombo, Domenico" uniqKey="Palombo D" first="Domenico" last="Palombo">Domenico Palombo</name><affiliation><nlm:affiliation>Vascular and Endovascular Surgery Unit, Research Laboratory of Experimental and Clinical Vascular Biology, DISC, Genoa University and IRCCS San Martino Hospital, Largo Rosanna Benzi, 16132, Genoa, Italy.</nlm:affiliation></affiliation></author><author><name sortKey="Converti, Attilio" sort="Converti, Attilio" uniqKey="Converti A" first="Attilio" last="Converti">Attilio Converti</name><affiliation><nlm:affiliation>Department of Civil, Chemical and Environmental Engineering, Pole of Chemical Engineering, Polytechnic School, Genoa University, Via Opera Pia 15, 16145, Genoa, Italy. converti@unige.it.</nlm:affiliation></affiliation></author><author><name sortKey="Polakiewicz, Bronislaw" sort="Polakiewicz, Bronislaw" uniqKey="Polakiewicz B" first="Bronislaw" last="Polakiewicz">Bronislaw Polakiewicz</name><affiliation><nlm:affiliation>Department of Biochemical and Pharmaceutical Technology, Faculty of Pharmaceutical Sciences, University of São Paulo, Av. Prof. Lineu Prestes, 580 B16, Cidade Universitária, São Paulo, SP, 05508-000, Brazil.</nlm:affiliation></affiliation></author></analytic><series><title level="j">Bioprocess and biosystems engineering</title><idno type="e-ISSN">1615-7605</idno><imprint><date when="2015" type="published">2015</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Many complications are associated to the therapeutic use of blood, among which are not only transfusion adverse events but also other issues such as lack of donors and high costs for collecting, testing, preserving, and distributing blood packages. Therefore, a clinically viable "blood substitute" is considered the holy grail of traumatology and may greatly benefit medicine. One of the most successful approaches to date is conjugating hemoglobin with polyethylene glycol (PEG). This conjugation aims mainly at overcoming free cell hemoglobin toxicity, which makes its use as oxygen carrier in pure form unfeasible. To improve PEG-hemoglobin conjugates feasibility, we propose applying dual functional PEG cross-linking hemoglobin molecules encapsulated by a protein carrier. The new oxygen carrier showed mean values of the hydrodynamic diameter, dispersity, and zeta potential of 1370 nm, 0.029 and -36 mV, respectively, evidencing the successful synthesis of PEG bis(N-succinimidyl succinate) and polyhemoglobin as well as the structuring of protein carrier.</div></front></TEI><pubmed><MedlineCitation Owner="NLM" Status="In-Process"><PMID Version="1">26314399</PMID><DateCreated><Year>2015</Year><Month>10</Month><Day>14</Day></DateCreated><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1615-7605</ISSN><JournalIssue CitedMedium="Internet"><Volume>38</Volume><Issue>11</Issue><PubDate><Year>2015</Year><Month>Nov</Month></PubDate></JournalIssue><Title>Bioprocess and biosystems engineering</Title><ISOAbbreviation>Bioprocess Biosyst Eng</ISOAbbreviation></Journal><ArticleTitle>Preparation and characterization of a microencapsulated polyethylene glycol cross-linked polyhemoglobin.</ArticleTitle><Pagination><MedlinePgn>2263-9</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1007/s00449-015-1463-y</ELocationID><Abstract><AbstractText>Many complications are associated to the therapeutic use of blood, among which are not only transfusion adverse events but also other issues such as lack of donors and high costs for collecting, testing, preserving, and distributing blood packages. Therefore, a clinically viable "blood substitute" is considered the holy grail of traumatology and may greatly benefit medicine. One of the most successful approaches to date is conjugating hemoglobin with polyethylene glycol (PEG). This conjugation aims mainly at overcoming free cell hemoglobin toxicity, which makes its use as oxygen carrier in pure form unfeasible. To improve PEG-hemoglobin conjugates feasibility, we propose applying dual functional PEG cross-linking hemoglobin molecules encapsulated by a protein carrier. The new oxygen carrier showed mean values of the hydrodynamic diameter, dispersity, and zeta potential of 1370 nm, 0.029 and -36 mV, respectively, evidencing the successful synthesis of PEG bis(N-succinimidyl succinate) and polyhemoglobin as well as the structuring of protein carrier.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Knirsch</LastName><ForeName>Marcos C</ForeName><Initials>MC</Initials><AffiliationInfo><Affiliation>Department of Biochemical and Pharmaceutical Technology, Faculty of Pharmaceutical Sciences, University of São Paulo, Av. Prof. Lineu Prestes, 580 B16, Cidade Universitária, São Paulo, SP, 05508-000, Brazil.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Capes Foundation, Ministry of Education of Brazil, Brasilia, DF, 70.040-020, Brazil.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Dell'Anno</LastName><ForeName>Filippo</ForeName><Initials>F</Initials><AffiliationInfo><Affiliation>Department of Civil, Chemical and Environmental Engineering, Pole of Chemical Engineering, Polytechnic School, Genoa University, Via Opera Pia 15, 16145, Genoa, Italy.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Chicoma</LastName><ForeName>Dennis</ForeName><Initials>D</Initials><AffiliationInfo><Affiliation>Chemical Engineering Department, Polytechnic School, University of São Paulo, Av. Prof. Lineu Prestes 580 B18, Cidade Universitária, São Paulo, SP, 05508-000, Brazil.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Stephano</LastName><ForeName>Marco Antonio</ForeName><Initials>MA</Initials><AffiliationInfo><Affiliation>Department of Biochemical and Pharmaceutical Technology, Faculty of Pharmaceutical Sciences, University of São Paulo, Av. Prof. Lineu Prestes, 580 B16, Cidade Universitária, São Paulo, SP, 05508-000, Brazil.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Bou-Chacra</LastName><ForeName>Nádia A</ForeName><Initials>NA</Initials><AffiliationInfo><Affiliation>Department of Pharmacy, Faculty of Pharmaceutical Sciences, University of São Paulo, Av. Prof. Lineu Prestes 580 B15, Cidade Universitária, São Paulo, SP, 05508-000, Brazil.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Palombo</LastName><ForeName>Domenico</ForeName><Initials>D</Initials><AffiliationInfo><Affiliation>Vascular and Endovascular Surgery Unit, Research Laboratory of Experimental and Clinical Vascular Biology, DISC, Genoa University and IRCCS San Martino Hospital, Largo Rosanna Benzi, 16132, Genoa, Italy.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Converti</LastName><ForeName>Attilio</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Department of Civil, Chemical and Environmental Engineering, Pole of Chemical Engineering, Polytechnic School, Genoa University, Via Opera Pia 15, 16145, Genoa, Italy. converti@unige.it.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Polakiewicz</LastName><ForeName>Bronislaw</ForeName><Initials>B</Initials><AffiliationInfo><Affiliation>Department of Biochemical and Pharmaceutical Technology, Faculty of Pharmaceutical Sciences, University of São Paulo, Av. Prof. Lineu Prestes, 580 B16, Cidade Universitária, São Paulo, SP, 05508-000, Brazil.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2015</Year><Month>08</Month><Day>28</Day></ArticleDate></Article><MedlineJournalInfo><Country>Germany</Country><MedlineTA>Bioprocess Biosyst Eng</MedlineTA><NlmUniqueID>101088505</NlmUniqueID><ISSNLinking>1615-7591</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Gelatin</Keyword><Keyword MajorTopicYN="N">Microparticle</Keyword><Keyword MajorTopicYN="N">Oxygen carrier</Keyword><Keyword MajorTopicYN="N">Polyethylene glycol</Keyword><Keyword MajorTopicYN="N">Polyhemoglobin</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2015</Year><Month>6</Month><Day>1</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2015</Year><Month>8</Month><Day>19</Day></PubMedPubDate><PubMedPubDate PubStatus="aheadofprint"><Year>2015</Year><Month>8</Month><Day>28</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2015</Year><Month>8</Month><Day>29</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2015</Year><Month>9</Month><Day>1</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2015</Year><Month>9</Month><Day>1</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">26314399</ArticleId><ArticleId IdType="doi">10.1007/s00449-015-1463-y</ArticleId><ArticleId IdType="pii">10.1007/s00449-015-1463-y</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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