OperaV1, PascalFrancis, Checkpoint, bibRecord, 000076

Development and Optimization of a Novel 384-Well Anti-Malarial Imaging Assay Validated for High-Throughput Screening

Identifieur interne : 000076 ( PascalFrancis/Checkpoint ); précédent : 000075; suivant : 000077

Development and Optimization of a Novel 384-Well Anti-Malarial Imaging Assay Validated for High-Throughput Screening

Auteurs : Sandra Duffy [Australie] ; Vicky M. Avery [Australie]

Source :

The American journal of tropical medicine and hygiene [ 0002-9637 ] ; 2012.

RBID : Pascal:12-0288826

Descripteurs français

Pascal (Inist)
- Optimisation, Antipaludique, Dépistage, Médecine tropicale, Antiparasitaire.

English descriptors

KwdEn :
- Antimalarial, Medical screening, Optimization, Parasiticide, Tropical medicine.

Abstract

With the increasing occurrence of drug resistance in the malaria parasite, Plasmodium falciparum, there is a great need for new and novel anti-malarial drugs. We have developed a 384-well, high-throughput imaging assay for the detection of new anti-malarial compounds, which was initially validated by screening a marine natural product library, and subsequently used to screen more than 3 million data points from a variety of compound sources. Founded on another fluorescence-based P. falciparum growth inhibition assay, the DNA-intercalating dye 4',6-diamidino-2-phenylindole, was used to monitor changes in parasite number. Fluorescent images were acquired on the PerkinElmer Opera High Throughput confocal imaging system and analyzed with a spot detection algorithm using the Acapella data processing software. Further optimization of this assay sought to increase throughput, assay stability, and compatibility with our high-throughput screening equipment platforms. The assay typically yielded Z'-factor values of 0.5-0.6, with signal-to-noise ratios of 12.

Affiliations:

Australie

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Pascal:12-0288826

Le document en format XML

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Development and Optimization of a Novel 384-Well Anti-Malarial Imaging Assay Validated for High-Throughput Screening

Development and Optimization of a Novel 384-Well Anti-Malarial Imaging Assay Validated for High-Throughput Screening

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Links to Exploration step

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri