VizPrimer: a web server for visualized PCR primer design based on known gene structure.
Identifieur interne : 000A41 ( Main/Exploration ); précédent : 000A40; suivant : 000A42VizPrimer: a web server for visualized PCR primer design based on known gene structure.
Auteurs : Yang Zhou [République populaire de Chine] ; Wubin Qu ; Yiming Lu ; Yanchun Zhang ; Xiaolei Wang ; Dongsheng Zhao ; Yi Yang ; Chenggang ZhangSource :
- Bioinformatics (Oxford, England) ; 2011.
English descriptors
- KwdEn :
- MESH :
- chemical , genetics : DNA Primers, Membrane Proteins, Nerve Tissue Proteins, Protein Isoforms.
- Animals, Exons, Humans, Mice, Open Reading Frames, Polymerase Chain Reaction, Polymorphism, Single Nucleotide.
Abstract
The visualization of gene structure plays an important role in polymerase chain reaction (PCR) primer design, especially for eukaryotic genes with a number of splice variants that users need to distinguish between via PCR. Here, we describe a visualized web server for primer design named VizPrimer. It utilizes the new information technology (IT) tools, HTML5 to display gene structure and JavaScript to interact with the users. In VizPrimer, the users can focus their attention on the gene structure and primer design strategy, without wasting time calculating the exon positions of splice variants or manually configuring complicated parameters. In addition, VizPrimer is also suitable for the design of PCR primers for amplifying open reading frames and detecting single nucleotide polymorphisms (SNPs).
DOI: 10.1093/bioinformatics/btr582
PubMed: 22016409
Affiliations:
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Le document en format XML
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<author><name sortKey="Zhao, Dongsheng" sort="Zhao, Dongsheng" uniqKey="Zhao D" first="Dongsheng" last="Zhao">Dongsheng Zhao</name>
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<author><name sortKey="Yang, Yi" sort="Yang, Yi" uniqKey="Yang Y" first="Yi" last="Yang">Yi Yang</name>
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<front><div type="abstract" xml:lang="en">The visualization of gene structure plays an important role in polymerase chain reaction (PCR) primer design, especially for eukaryotic genes with a number of splice variants that users need to distinguish between via PCR. Here, we describe a visualized web server for primer design named VizPrimer. It utilizes the new information technology (IT) tools, HTML5 to display gene structure and JavaScript to interact with the users. In VizPrimer, the users can focus their attention on the gene structure and primer design strategy, without wasting time calculating the exon positions of splice variants or manually configuring complicated parameters. In addition, VizPrimer is also suitable for the design of PCR primers for amplifying open reading frames and detecting single nucleotide polymorphisms (SNPs).</div>
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<name sortKey="Wang, Xiaolei" sort="Wang, Xiaolei" uniqKey="Wang X" first="Xiaolei" last="Wang">Xiaolei Wang</name>
<name sortKey="Yang, Yi" sort="Yang, Yi" uniqKey="Yang Y" first="Yi" last="Yang">Yi Yang</name>
<name sortKey="Zhang, Chenggang" sort="Zhang, Chenggang" uniqKey="Zhang C" first="Chenggang" last="Zhang">Chenggang Zhang</name>
<name sortKey="Zhang, Yanchun" sort="Zhang, Yanchun" uniqKey="Zhang Y" first="Yanchun" last="Zhang">Yanchun Zhang</name>
<name sortKey="Zhao, Dongsheng" sort="Zhao, Dongsheng" uniqKey="Zhao D" first="Dongsheng" last="Zhao">Dongsheng Zhao</name>
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<country name="République populaire de Chine"><noRegion><name sortKey="Zhou, Yang" sort="Zhou, Yang" uniqKey="Zhou Y" first="Yang" last="Zhou">Yang Zhou</name>
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