Human osteoblast-like cells response to nanofunctionalized surfaces for tissue engineering.
Identifieur interne : 001369 ( Main/Exploration ); précédent : 001368; suivant : 001370Human osteoblast-like cells response to nanofunctionalized surfaces for tissue engineering.
Auteurs : Federico Caneva Soumetz [Italie] ; Laura Pastorino ; Carmelina RuggieroSource :
- Journal of biomedical materials research. Part B, Applied biomaterials [ 1552-4973 ] ; 2008.
English descriptors
- KwdEn :
- Biocompatible Materials, Biomimetic Materials, Cell Line, Extracellular Matrix (ultrastructure), Fibronectins (chemistry), Glass (chemistry), Humans, Microscopy, Atomic Force, Nanotechnology, Nickel (chemistry), Osteoblasts (physiology), Silicon (chemistry), Surface Properties, Tissue Engineering, Titanium (chemistry).
- MESH :
- chemical , chemistry : Fibronectins, Nickel, Silicon, Titanium.
- chemical : Biocompatible Materials.
- chemistry : Glass.
- physiology : Osteoblasts.
- ultrastructure : Extracellular Matrix.
- Biomimetic Materials, Cell Line, Humans, Microscopy, Atomic Force, Nanotechnology, Surface Properties, Tissue Engineering.
Abstract
Cells are sensitive both to the micro/nanotopographic and chemical features of their surrounding environment. The engineering of the surface properties of biomaterials is then critical to develop bioactive devices with which to elicit appropriate cellular responses. To this regard, the layer by layer (LBL) self assembly technique represents a simple and versatile method to modify surface properties by the deposition of ultrathin films with specific and predetermined properties. In this work biomimetic coatings containing fibronectin, an adhesive glycoprotein of the extracellular matrix, were assembled by means of the LBL technique, and tested for the growth of MG63 human osteoblast-like cells, in order to evaluate their potential for the treatment of materials employed in bone-tissue engineering. As a first step the assembly process was optimized by quartz crystal microbalance measurements and subsequently was repeated on nickel/titanium, silicon and glass samples. The results obtained from the investigation of cell response to the modified surfaces, put in evidence that the deposited nanostructured ultrathin films are effective in promoting cell proliferation. Our results show the high potential of the developed bioactive coatings for the engineering of biomimetic implants and for the optimization of their integration with the surrounding tissues.
DOI: 10.1002/jbm.b.30867
PubMed: 17514672
Affiliations:
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Human osteoblast-like cells response to nanofunctionalized surfaces for tissue engineering.</title><author><name sortKey="Soumetz, Federico Caneva" sort="Soumetz, Federico Caneva" uniqKey="Soumetz F" first="Federico Caneva" last="Soumetz">Federico Caneva Soumetz</name><affiliation wicri:level="1"><nlm:affiliation>Department of Communication, Computer and System Sciences, University of Genova, Via Opera Pia, 13-16145 Genova, Italy. fedecnv@dist.unige.it</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>Department of Communication, Computer and System Sciences, University of Genova, Via Opera Pia, 13-16145 Genova</wicri:regionArea><wicri:noRegion>13-16145 Genova</wicri:noRegion></affiliation></author><author><name sortKey="Pastorino, Laura" sort="Pastorino, Laura" uniqKey="Pastorino L" first="Laura" last="Pastorino">Laura Pastorino</name></author><author><name sortKey="Ruggiero, Carmelina" sort="Ruggiero, Carmelina" uniqKey="Ruggiero C" first="Carmelina" last="Ruggiero">Carmelina Ruggiero</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2008">2008</date><idno type="doi">10.1002/jbm.b.30867</idno><idno type="RBID">pubmed:17514672</idno><idno type="pmid">17514672</idno><idno type="wicri:Area/PubMed/Corpus">000460</idno><idno type="wicri:Area/PubMed/Curation">000460</idno><idno type="wicri:Area/PubMed/Checkpoint">000417</idno><idno type="wicri:Area/Ncbi/Merge">000432</idno><idno type="wicri:Area/Ncbi/Curation">000432</idno><idno type="wicri:Area/Ncbi/Checkpoint">000432</idno><idno type="wicri:doubleKey">1552-4973:2008:Soumetz F:human:osteoblast:like</idno><idno type="wicri:Area/Main/Merge">001386</idno><idno type="wicri:Area/Main/Curation">001369</idno><idno type="wicri:Area/Main/Exploration">001369</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Human osteoblast-like cells response to nanofunctionalized surfaces for tissue engineering.</title><author><name sortKey="Soumetz, Federico Caneva" sort="Soumetz, Federico Caneva" uniqKey="Soumetz F" first="Federico Caneva" last="Soumetz">Federico Caneva Soumetz</name><affiliation wicri:level="1"><nlm:affiliation>Department of Communication, Computer and System Sciences, University of Genova, Via Opera Pia, 13-16145 Genova, Italy. fedecnv@dist.unige.it</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>Department of Communication, Computer and System Sciences, University of Genova, Via Opera Pia, 13-16145 Genova</wicri:regionArea><wicri:noRegion>13-16145 Genova</wicri:noRegion></affiliation></author><author><name sortKey="Pastorino, Laura" sort="Pastorino, Laura" uniqKey="Pastorino L" first="Laura" last="Pastorino">Laura Pastorino</name></author><author><name sortKey="Ruggiero, Carmelina" sort="Ruggiero, Carmelina" uniqKey="Ruggiero C" first="Carmelina" last="Ruggiero">Carmelina Ruggiero</name></author></analytic><series><title level="j">Journal of biomedical materials research. Part B, Applied biomaterials</title><idno type="ISSN">1552-4973</idno><imprint><date when="2008" type="published">2008</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Biocompatible Materials</term><term>Biomimetic Materials</term><term>Cell Line</term><term>Extracellular Matrix (ultrastructure)</term><term>Fibronectins (chemistry)</term><term>Glass (chemistry)</term><term>Humans</term><term>Microscopy, Atomic Force</term><term>Nanotechnology</term><term>Nickel (chemistry)</term><term>Osteoblasts (physiology)</term><term>Silicon (chemistry)</term><term>Surface Properties</term><term>Tissue Engineering</term><term>Titanium (chemistry)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Fibronectins</term><term>Nickel</term><term>Silicon</term><term>Titanium</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>Biocompatible Materials</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Glass</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Osteoblasts</term></keywords><keywords scheme="MESH" qualifier="ultrastructure" xml:lang="en"><term>Extracellular Matrix</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Biomimetic Materials</term><term>Cell Line</term><term>Humans</term><term>Microscopy, Atomic Force</term><term>Nanotechnology</term><term>Surface Properties</term><term>Tissue Engineering</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Cells are sensitive both to the micro/nanotopographic and chemical features of their surrounding environment. The engineering of the surface properties of biomaterials is then critical to develop bioactive devices with which to elicit appropriate cellular responses. To this regard, the layer by layer (LBL) self assembly technique represents a simple and versatile method to modify surface properties by the deposition of ultrathin films with specific and predetermined properties. In this work biomimetic coatings containing fibronectin, an adhesive glycoprotein of the extracellular matrix, were assembled by means of the LBL technique, and tested for the growth of MG63 human osteoblast-like cells, in order to evaluate their potential for the treatment of materials employed in bone-tissue engineering. As a first step the assembly process was optimized by quartz crystal microbalance measurements and subsequently was repeated on nickel/titanium, silicon and glass samples. The results obtained from the investigation of cell response to the modified surfaces, put in evidence that the deposited nanostructured ultrathin films are effective in promoting cell proliferation. Our results show the high potential of the developed bioactive coatings for the engineering of biomimetic implants and for the optimization of their integration with the surrounding tissues.</div></front></TEI><affiliations><list><country><li>Italie</li></country></list><tree><noCountry><name sortKey="Pastorino, Laura" sort="Pastorino, Laura" uniqKey="Pastorino L" first="Laura" last="Pastorino">Laura Pastorino</name><name sortKey="Ruggiero, Carmelina" sort="Ruggiero, Carmelina" uniqKey="Ruggiero C" first="Carmelina" last="Ruggiero">Carmelina Ruggiero</name></noCountry><country name="Italie"><noRegion><name sortKey="Soumetz, Federico Caneva" sort="Soumetz, Federico Caneva" uniqKey="Soumetz F" first="Federico Caneva" last="Soumetz">Federico Caneva Soumetz</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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