Kinetic and thermodynamic studies of a novel acid protease from Aspergillus foetidus.
Identifieur interne : 000178 ( Main/Exploration ); précédent : 000177; suivant : 000179Kinetic and thermodynamic studies of a novel acid protease from Aspergillus foetidus.
Auteurs : Paula Monteiro Souza [Brésil] ; Bahar Aliakbarian [Italie] ; Edivaldo Ximenes Ferreira Filho [Brésil] ; Pérola Oliveira Magalhães [Brésil] ; Adalberto Pessoa Junior [Brésil] ; Attilio Converti [Italie] ; Patrizia Perego [Italie]Source :
- International journal of biological macromolecules ; 2015.
Abstract
The kinetics of a thermostable extracellular acid protease produced by an Aspergillus foetidus strain was investigated at different pH, temperatures and substrate concentrations. The enzyme exhibited maximal activity at pH 5.0 and 55°C, and its irreversible deactivation was well described by first-order kinetics. When temperature was raised from 55 to 70°C, the deactivation rate constant increased from 0.018 to 5.06h(-1), while the half-life decreased from 37.6 to 0.13h. The results of activity collected at different temperatures were then used to estimate, the activation energy of the hydrolysis reaction (E*=19.03kJ/mol) and the standard enthalpy variation of reversible enzyme unfolding (ΔH°U=19.03kJ/mol). The results of residual activity tests carried out in the temperature range 55-70°C allowed estimating the activation energy (E(*)d=314.12kJ/mol), enthalpy (311.27≤(ΔH°d≤311.39kJ/mol), entropy (599.59≤ΔS(*)d≤610.49kJ/mol K) and Gibbs free energy (103.18≤ΔG(*)d≤113.87kJ/mol) of the enzyme irreversible denaturation. These thermodynamic parameters suggest that this new protease is highly thermostable and could be important for industrial applications. To the best of our knowledge, this is the first report on thermodynamic parameters of an acid protease produced by A. foetidus.
DOI: 10.1016/j.ijbiomac.2015.07.043
PubMed: 26210038
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: 000063
- to stream PubMed, to step Curation: 000063
- to stream PubMed, to step Checkpoint: 000085
- to stream Ncbi, to step Merge: 000D85
- to stream Ncbi, to step Curation: 000D85
- to stream Ncbi, to step Checkpoint: 000D85
- to stream Main, to step Merge: 000175
- to stream Main, to step Curation: 000178
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Kinetic and thermodynamic studies of a novel acid protease from Aspergillus foetidus.</title><author><name sortKey="Souza, Paula Monteiro" sort="Souza, Paula Monteiro" uniqKey="Souza P" first="Paula Monteiro" last="Souza">Paula Monteiro Souza</name><affiliation wicri:level="4"><nlm:affiliation>Department of Biochemical and Pharmaceutical Technology, University of São Paulo, São Paulo, SP, 05508-000, Brazil. Electronic address: paulasouza@usp.br.</nlm:affiliation><country xml:lang="fr">Brésil</country><wicri:regionArea>Department of Biochemical and Pharmaceutical Technology, University of São Paulo, São Paulo, SP, 05508-000</wicri:regionArea><orgName type="university">Université de São Paulo</orgName><placeName><settlement type="city">São Paulo</settlement><region type="state">État de São Paulo</region></placeName></affiliation></author><author><name sortKey="Aliakbarian, Bahar" sort="Aliakbarian, Bahar" uniqKey="Aliakbarian B" first="Bahar" last="Aliakbarian">Bahar Aliakbarian</name><affiliation wicri:level="1"><nlm:affiliation>Department of Civil, Chemical and Environmental Engineering, Pole of Chemical Engineering, Genoa University, via Opera Pia 15, 16145 Genoa, Italy.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>Department of Civil, Chemical and Environmental Engineering, Pole of Chemical Engineering, Genoa University, via Opera Pia 15, 16145 Genoa</wicri:regionArea><wicri:noRegion>16145 Genoa</wicri:noRegion></affiliation></author><author><name sortKey="Filho, Edivaldo Ximenes Ferreira" sort="Filho, Edivaldo Ximenes Ferreira" uniqKey="Filho E" first="Edivaldo Ximenes Ferreira" last="Filho">Edivaldo Ximenes Ferreira Filho</name><affiliation wicri:level="1"><nlm:affiliation>Laboratory of Enzymology, Department of Cellular Biology, University of Brasilia, Campus Darcy Ribeiro, Asa Norte, Brasilia, DF, 70910900, Brazil.</nlm:affiliation><country xml:lang="fr">Brésil</country><wicri:regionArea>Laboratory of Enzymology, Department of Cellular Biology, University of Brasilia, Campus Darcy Ribeiro, Asa Norte, Brasilia, DF, 70910900</wicri:regionArea><wicri:noRegion>70910900</wicri:noRegion></affiliation></author><author><name sortKey="Magalhaes, Perola Oliveira" sort="Magalhaes, Perola Oliveira" uniqKey="Magalhaes P" first="Pérola Oliveira" last="Magalhães">Pérola Oliveira Magalhães</name><affiliation wicri:level="1"><nlm:affiliation>Department of Pharmaceutical Sciences, School of Health Sciences, University of Brasilia, Campus Darcy Ribeiro, Asa Norte, Brasilia, DF, 70910900, Brazil.</nlm:affiliation><country xml:lang="fr">Brésil</country><wicri:regionArea>Department of Pharmaceutical Sciences, School of Health Sciences, University of Brasilia, Campus Darcy Ribeiro, Asa Norte, Brasilia, DF, 70910900</wicri:regionArea><wicri:noRegion>70910900</wicri:noRegion></affiliation></author><author><name sortKey="Junior, Adalberto Pessoa" sort="Junior, Adalberto Pessoa" uniqKey="Junior A" first="Adalberto Pessoa" last="Junior">Adalberto Pessoa Junior</name><affiliation wicri:level="4"><nlm:affiliation>Department of Biochemical and Pharmaceutical Technology, University of São Paulo, São Paulo, SP, 05508-000, Brazil.</nlm:affiliation><country xml:lang="fr">Brésil</country><wicri:regionArea>Department of Biochemical and Pharmaceutical Technology, University of São Paulo, São Paulo, SP, 05508-000</wicri:regionArea><orgName type="university">Université de São Paulo</orgName><placeName><settlement type="city">São Paulo</settlement><region type="state">État de São Paulo</region></placeName></affiliation></author><author><name sortKey="Converti, Attilio" sort="Converti, Attilio" uniqKey="Converti A" first="Attilio" last="Converti">Attilio Converti</name><affiliation wicri:level="1"><nlm:affiliation>Department of Civil, Chemical and Environmental Engineering, Pole of Chemical Engineering, Genoa University, via Opera Pia 15, 16145 Genoa, Italy.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>Department of Civil, Chemical and Environmental Engineering, Pole of Chemical Engineering, Genoa University, via Opera Pia 15, 16145 Genoa</wicri:regionArea><wicri:noRegion>16145 Genoa</wicri:noRegion></affiliation></author><author><name sortKey="Perego, Patrizia" sort="Perego, Patrizia" uniqKey="Perego P" first="Patrizia" last="Perego">Patrizia Perego</name><affiliation wicri:level="1"><nlm:affiliation>Department of Civil, Chemical and Environmental Engineering, Pole of Chemical Engineering, Genoa University, via Opera Pia 15, 16145 Genoa, Italy.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>Department of Civil, Chemical and Environmental Engineering, Pole of Chemical Engineering, Genoa University, via Opera Pia 15, 16145 Genoa</wicri:regionArea><wicri:noRegion>16145 Genoa</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2015">2015</date><idno type="RBID">pubmed:26210038</idno><idno type="pmid">26210038</idno><idno type="doi">10.1016/j.ijbiomac.2015.07.043</idno><idno type="wicri:Area/PubMed/Corpus">000063</idno><idno type="wicri:Area/PubMed/Curation">000063</idno><idno type="wicri:Area/PubMed/Checkpoint">000085</idno><idno type="wicri:Area/Ncbi/Merge">000D85</idno><idno type="wicri:Area/Ncbi/Curation">000D85</idno><idno type="wicri:Area/Ncbi/Checkpoint">000D85</idno><idno type="wicri:Area/Main/Merge">000175</idno><idno type="wicri:Area/Main/Curation">000178</idno><idno type="wicri:Area/Main/Exploration">000178</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Kinetic and thermodynamic studies of a novel acid protease from Aspergillus foetidus.</title><author><name sortKey="Souza, Paula Monteiro" sort="Souza, Paula Monteiro" uniqKey="Souza P" first="Paula Monteiro" last="Souza">Paula Monteiro Souza</name><affiliation wicri:level="4"><nlm:affiliation>Department of Biochemical and Pharmaceutical Technology, University of São Paulo, São Paulo, SP, 05508-000, Brazil. Electronic address: paulasouza@usp.br.</nlm:affiliation><country xml:lang="fr">Brésil</country><wicri:regionArea>Department of Biochemical and Pharmaceutical Technology, University of São Paulo, São Paulo, SP, 05508-000</wicri:regionArea><orgName type="university">Université de São Paulo</orgName><placeName><settlement type="city">São Paulo</settlement><region type="state">État de São Paulo</region></placeName></affiliation></author><author><name sortKey="Aliakbarian, Bahar" sort="Aliakbarian, Bahar" uniqKey="Aliakbarian B" first="Bahar" last="Aliakbarian">Bahar Aliakbarian</name><affiliation wicri:level="1"><nlm:affiliation>Department of Civil, Chemical and Environmental Engineering, Pole of Chemical Engineering, Genoa University, via Opera Pia 15, 16145 Genoa, Italy.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>Department of Civil, Chemical and Environmental Engineering, Pole of Chemical Engineering, Genoa University, via Opera Pia 15, 16145 Genoa</wicri:regionArea><wicri:noRegion>16145 Genoa</wicri:noRegion></affiliation></author><author><name sortKey="Filho, Edivaldo Ximenes Ferreira" sort="Filho, Edivaldo Ximenes Ferreira" uniqKey="Filho E" first="Edivaldo Ximenes Ferreira" last="Filho">Edivaldo Ximenes Ferreira Filho</name><affiliation wicri:level="1"><nlm:affiliation>Laboratory of Enzymology, Department of Cellular Biology, University of Brasilia, Campus Darcy Ribeiro, Asa Norte, Brasilia, DF, 70910900, Brazil.</nlm:affiliation><country xml:lang="fr">Brésil</country><wicri:regionArea>Laboratory of Enzymology, Department of Cellular Biology, University of Brasilia, Campus Darcy Ribeiro, Asa Norte, Brasilia, DF, 70910900</wicri:regionArea><wicri:noRegion>70910900</wicri:noRegion></affiliation></author><author><name sortKey="Magalhaes, Perola Oliveira" sort="Magalhaes, Perola Oliveira" uniqKey="Magalhaes P" first="Pérola Oliveira" last="Magalhães">Pérola Oliveira Magalhães</name><affiliation wicri:level="1"><nlm:affiliation>Department of Pharmaceutical Sciences, School of Health Sciences, University of Brasilia, Campus Darcy Ribeiro, Asa Norte, Brasilia, DF, 70910900, Brazil.</nlm:affiliation><country xml:lang="fr">Brésil</country><wicri:regionArea>Department of Pharmaceutical Sciences, School of Health Sciences, University of Brasilia, Campus Darcy Ribeiro, Asa Norte, Brasilia, DF, 70910900</wicri:regionArea><wicri:noRegion>70910900</wicri:noRegion></affiliation></author><author><name sortKey="Junior, Adalberto Pessoa" sort="Junior, Adalberto Pessoa" uniqKey="Junior A" first="Adalberto Pessoa" last="Junior">Adalberto Pessoa Junior</name><affiliation wicri:level="4"><nlm:affiliation>Department of Biochemical and Pharmaceutical Technology, University of São Paulo, São Paulo, SP, 05508-000, Brazil.</nlm:affiliation><country xml:lang="fr">Brésil</country><wicri:regionArea>Department of Biochemical and Pharmaceutical Technology, University of São Paulo, São Paulo, SP, 05508-000</wicri:regionArea><orgName type="university">Université de São Paulo</orgName><placeName><settlement type="city">São Paulo</settlement><region type="state">État de São Paulo</region></placeName></affiliation></author><author><name sortKey="Converti, Attilio" sort="Converti, Attilio" uniqKey="Converti A" first="Attilio" last="Converti">Attilio Converti</name><affiliation wicri:level="1"><nlm:affiliation>Department of Civil, Chemical and Environmental Engineering, Pole of Chemical Engineering, Genoa University, via Opera Pia 15, 16145 Genoa, Italy.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>Department of Civil, Chemical and Environmental Engineering, Pole of Chemical Engineering, Genoa University, via Opera Pia 15, 16145 Genoa</wicri:regionArea><wicri:noRegion>16145 Genoa</wicri:noRegion></affiliation></author><author><name sortKey="Perego, Patrizia" sort="Perego, Patrizia" uniqKey="Perego P" first="Patrizia" last="Perego">Patrizia Perego</name><affiliation wicri:level="1"><nlm:affiliation>Department of Civil, Chemical and Environmental Engineering, Pole of Chemical Engineering, Genoa University, via Opera Pia 15, 16145 Genoa, Italy.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>Department of Civil, Chemical and Environmental Engineering, Pole of Chemical Engineering, Genoa University, via Opera Pia 15, 16145 Genoa</wicri:regionArea><wicri:noRegion>16145 Genoa</wicri:noRegion></affiliation></author></analytic><series><title level="j">International journal of biological macromolecules</title><idno type="e-ISSN">1879-0003</idno><imprint><date when="2015" type="published">2015</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The kinetics of a thermostable extracellular acid protease produced by an Aspergillus foetidus strain was investigated at different pH, temperatures and substrate concentrations. The enzyme exhibited maximal activity at pH 5.0 and 55°C, and its irreversible deactivation was well described by first-order kinetics. When temperature was raised from 55 to 70°C, the deactivation rate constant increased from 0.018 to 5.06h(-1), while the half-life decreased from 37.6 to 0.13h. The results of activity collected at different temperatures were then used to estimate, the activation energy of the hydrolysis reaction (E*=19.03kJ/mol) and the standard enthalpy variation of reversible enzyme unfolding (ΔH°U=19.03kJ/mol). The results of residual activity tests carried out in the temperature range 55-70°C allowed estimating the activation energy (E(*)d=314.12kJ/mol), enthalpy (311.27≤(ΔH°d≤311.39kJ/mol), entropy (599.59≤ΔS(*)d≤610.49kJ/mol K) and Gibbs free energy (103.18≤ΔG(*)d≤113.87kJ/mol) of the enzyme irreversible denaturation. These thermodynamic parameters suggest that this new protease is highly thermostable and could be important for industrial applications. To the best of our knowledge, this is the first report on thermodynamic parameters of an acid protease produced by A. foetidus.</div></front></TEI><affiliations><list><country><li>Brésil</li><li>Italie</li></country><region><li>État de São Paulo</li></region><settlement><li>São Paulo</li></settlement><orgName><li>Université de São Paulo</li></orgName></list><tree><country name="Brésil"><region name="État de São Paulo"><name sortKey="Souza, Paula Monteiro" sort="Souza, Paula Monteiro" uniqKey="Souza P" first="Paula Monteiro" last="Souza">Paula Monteiro Souza</name></region><name sortKey="Filho, Edivaldo Ximenes Ferreira" sort="Filho, Edivaldo Ximenes Ferreira" uniqKey="Filho E" first="Edivaldo Ximenes Ferreira" last="Filho">Edivaldo Ximenes Ferreira Filho</name><name sortKey="Junior, Adalberto Pessoa" sort="Junior, Adalberto Pessoa" uniqKey="Junior A" first="Adalberto Pessoa" last="Junior">Adalberto Pessoa Junior</name><name sortKey="Magalhaes, Perola Oliveira" sort="Magalhaes, Perola Oliveira" uniqKey="Magalhaes P" first="Pérola Oliveira" last="Magalhães">Pérola Oliveira Magalhães</name></country><country name="Italie"><noRegion><name sortKey="Aliakbarian, Bahar" sort="Aliakbarian, Bahar" uniqKey="Aliakbarian B" first="Bahar" last="Aliakbarian">Bahar Aliakbarian</name></noRegion><name sortKey="Converti, Attilio" sort="Converti, Attilio" uniqKey="Converti A" first="Attilio" last="Converti">Attilio Converti</name><name sortKey="Perego, Patrizia" sort="Perego, Patrizia" uniqKey="Perego P" first="Patrizia" last="Perego">Patrizia Perego</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Musique/explor/OperaV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000178 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000178 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Musique
|area= OperaV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:26210038
|texte= Kinetic and thermodynamic studies of a novel acid protease from Aspergillus foetidus.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:26210038" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a OperaV1
| This area was generated with Dilib version V0.6.21. |  |