OperaV1, Main, Exploration, bibRecord, 000138

Probing poly(N-isopropylacrylamide-co-butylacrylate)/cell interactions by atomic force microscopy.

Identifieur interne : 000138 ( Main/Exploration ); précédent : 000137; suivant : 000139

Probing poly(N-isopropylacrylamide-co-butylacrylate)/cell interactions by atomic force microscopy.

Auteurs : Becerra Natalia [Colombie] ; Andrade Henry ; L Pez Betty ; Restrepo Luz Marina ; Raiteri Roberto

Source :

Journal of biomedical materials research. Part A ; 2015.

RBID : pubmed:24619976

English descriptors

KwdEn :
- Acrylic Resins (chemistry), Animals, Mice, Microscopy, Atomic Force (methods), Molecular Probes, NIH 3T3 Cells.
MESH :
- chemical , chemistry : Acrylic Resins.
- methods : Microscopy, Atomic Force.
- Animals, Mice, Molecular Probes, NIH 3T3 Cells.

Abstract

Poly(N-isopropylacrylamide) based hydrogels have been proposed as cell culture supports in cell sheet engineering. Toward this goal, we characterized the poly(N-isopropylacrylamide-co-butylacrylate) copolymer thermo-sensitivity and the cell/copolymer interactions above and below the copolymer lower critical solution temperature. We did that by direct force measurements at different temperatures using an atomic force microscope with either a polystyrene or a glass microbead as probes. We used a copolymer-coated microbead to measure adhesion after a short contact time with a single fibroblast in culture. Statistical analysis of the maximum adhesion force and the mechanical work necessary to separate the probe from the cell surface confirmed the hydrophilic/hydrophobic behavior of poly(N-isopropylacrylamide-co-butylacrylate) as a function of temperature in the range 20-37°C and, consequently, a reversible increase/decrease in cell adhesion with the copolymer. As control experiments we measured interactions between uncoated microbeads with the copolymer hydrogel or cells as well as interaction of the Poly(N-isopropylacrylamide) homopolymer with cells. These results show the potential of an assay based on atomic force microscopy for an in situ and quantitative assessment of cell/substrate interactions and support the use of poly(N-isopropylacrylamide-co-butylacrylate) copolymer as an efficient culture substrate in cell sheet engineering.

DOI: 10.1002/jbm.a.35163
PubMed: 24619976

Affiliations:

Colombie

Le document en format XML

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<sourceDesc><biblStruct><analytic><title xml:lang="en">Probing poly(N-isopropylacrylamide-co-butylacrylate)/cell interactions by atomic force microscopy.</title>
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<affiliation wicri:level="1"><nlm:affiliation>Department of Informatics Bioengineering, Robotics and System Engineering (DIBRIS), University of Genova, Via Opera Pia, 13, Genova, Italy; Grupo Ciencia de Materiales. Instituto de Química, Facultad de Ciencias Exactas y Naturales, Universidad de Antioquia UdeA, Calle 70 No 52-21, Medellín, Colombia; Grupo Ingeniería de Tejidos y Terapia celular Facultad de Medicina Laboratorio Terapia celular y Biobanco, IPS Universitaria, Universidad de Antioquia UdeA, Calle 70 No 52-21, Medellín, Colombia.</nlm:affiliation>
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<series><title level="j">Journal of biomedical materials research. Part A</title>
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<front><div type="abstract" xml:lang="en">Poly(N-isopropylacrylamide) based hydrogels have been proposed as cell culture supports in cell sheet engineering. Toward this goal, we characterized the poly(N-isopropylacrylamide-co-butylacrylate) copolymer thermo-sensitivity and the cell/copolymer interactions above and below the copolymer lower critical solution temperature. We did that by direct force measurements at different temperatures using an atomic force microscope with either a polystyrene or a glass microbead as probes. We used a copolymer-coated microbead to measure adhesion after a short contact time with a single fibroblast in culture. Statistical analysis of the maximum adhesion force and the mechanical work necessary to separate the probe from the cell surface confirmed the hydrophilic/hydrophobic behavior of poly(N-isopropylacrylamide-co-butylacrylate) as a function of temperature in the range 20-37°C and, consequently, a reversible increase/decrease in cell adhesion with the copolymer. As control experiments we measured interactions between uncoated microbeads with the copolymer hydrogel or cells as well as interaction of the Poly(N-isopropylacrylamide) homopolymer with cells. These results show the potential of an assay based on atomic force microscopy for an in situ and quantitative assessment of cell/substrate interactions and support the use of poly(N-isopropylacrylamide-co-butylacrylate) copolymer as an efficient culture substrate in cell sheet engineering.</div>
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Probing poly(N-isopropylacrylamide-co-butylacrylate)/cell interactions by atomic force microscopy.

Probing poly(N-isopropylacrylamide-co-butylacrylate)/cell interactions by atomic force microscopy.

Source :

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

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Pour mettre un lien sur cette page dans le réseau Wicri

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