Synthesis of ethyl phenylacetate by lyophilized mycelium of Aspergillus oryzae.
Identifieur interne : 001A00 ( Main/Curation ); précédent : 001999; suivant : 001A01Synthesis of ethyl phenylacetate by lyophilized mycelium of Aspergillus oryzae.
Auteurs : A. Converti [Italie] ; R. Gandolfi ; M. Zilli ; F. Molinari ; L. Binaghi ; P. Perego ; M. Del BorghiSource :
- Applied microbiology and biotechnology [ 0175-7598 ] ; 2005.
English descriptors
- KwdEn :
- MESH :
- chemical , metabolism : Carboxylesterase, Ethanol, Phenylacetates.
- metabolism : Aspergillus oryzae, Mycelium, Rhizopus.
- Acylation, Biotransformation, Catalysis, Freeze Drying, Kinetics, Temperature, Thermodynamics.
Abstract
Lyophilized mycelia of Aspergillus oryzae CBS 102.07, Aspergillus oryzae MIM, Rhizopus oryzae CBS 112.07, Rhizopus oryzae CBS 391.34, Rhizopus oryzae CBS 260.28 and Rhizopus oryzae CBS 328.47 were tested in this study to select the best biocatalysts for ethanol acylation with phenylacetic acid. The mycelium-bound carboxylesterase activity of A. oryzae MIM, which exhibited the best performances, was initially investigated at 50 degrees C, either in 0.1 M phosphate buffer or in n-heptane to catalyse the hydrolysis or the synthesis, respectively, of ethyl phenylacetate. The results in terms of product and substrate concentrations versus time were used to estimate the maximum molar conversions at equilibrium, the equilibrium constants, and the times needed to reach half maximum conversions, thus providing sufficient information about this biotransformation. The values of the apparent equilibrium constants, estimated at 20 degrees C
DOI: 10.1007/s00253-004-1830-0
PubMed: 15650849
DOI: 10.1007/s00253-004-1830-0
PubMed: 15650849
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: Pour aller vers cette notice dans l'étape Curation :000532
- to stream PubMed, to step Curation: Pour aller vers cette notice dans l'étape Curation :000532
- to stream PubMed, to step Checkpoint: Pour aller vers cette notice dans l'étape Curation :000487
- to stream Ncbi, to step Merge: Pour aller vers cette notice dans l'étape Curation :000321
- to stream Ncbi, to step Curation: Pour aller vers cette notice dans l'étape Curation :000321
- to stream Ncbi, to step Checkpoint: Pour aller vers cette notice dans l'étape Curation :000321
- to stream Main, to step Merge: Pour aller vers cette notice dans l'étape Curation :001A39
Links to Exploration step
pubmed:15650849Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Synthesis of ethyl phenylacetate by lyophilized mycelium of Aspergillus oryzae.</title>
<author><name sortKey="Converti, A" sort="Converti, A" uniqKey="Converti A" first="A" last="Converti">A. Converti</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Chemical and Process Engineering, University of Genoa, via Opera Pia 15, 16145 Genoa, Italy. converti@unige.it</nlm:affiliation>
<country xml:lang="fr">Italie</country>
<wicri:regionArea>Department of Chemical and Process Engineering, University of Genoa, via Opera Pia 15, 16145 Genoa</wicri:regionArea>
<wicri:noRegion>16145 Genoa</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Gandolfi, R" sort="Gandolfi, R" uniqKey="Gandolfi R" first="R" last="Gandolfi">R. Gandolfi</name>
</author>
<author><name sortKey="Zilli, M" sort="Zilli, M" uniqKey="Zilli M" first="M" last="Zilli">M. Zilli</name>
</author>
<author><name sortKey="Molinari, F" sort="Molinari, F" uniqKey="Molinari F" first="F" last="Molinari">F. Molinari</name>
</author>
<author><name sortKey="Binaghi, L" sort="Binaghi, L" uniqKey="Binaghi L" first="L" last="Binaghi">L. Binaghi</name>
</author>
<author><name sortKey="Perego, P" sort="Perego, P" uniqKey="Perego P" first="P" last="Perego">P. Perego</name>
</author>
<author><name sortKey="Del Borghi, M" sort="Del Borghi, M" uniqKey="Del Borghi M" first="M" last="Del Borghi">M. Del Borghi</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PubMed</idno>
<date when="2005">2005</date>
<idno type="doi">10.1007/s00253-004-1830-0</idno>
<idno type="RBID">pubmed:15650849</idno>
<idno type="pmid">15650849</idno>
<idno type="wicri:Area/PubMed/Corpus">000532</idno>
<idno type="wicri:Area/PubMed/Curation">000532</idno>
<idno type="wicri:Area/PubMed/Checkpoint">000487</idno>
<idno type="wicri:Area/Ncbi/Merge">000321</idno>
<idno type="wicri:Area/Ncbi/Curation">000321</idno>
<idno type="wicri:Area/Ncbi/Checkpoint">000321</idno>
<idno type="wicri:doubleKey">0175-7598:2005:Converti A:synthesis:of:ethyl</idno>
<idno type="wicri:Area/Main/Merge">001A39</idno>
<idno type="wicri:Area/Main/Curation">001A00</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en">Synthesis of ethyl phenylacetate by lyophilized mycelium of Aspergillus oryzae.</title>
<author><name sortKey="Converti, A" sort="Converti, A" uniqKey="Converti A" first="A" last="Converti">A. Converti</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Chemical and Process Engineering, University of Genoa, via Opera Pia 15, 16145 Genoa, Italy. converti@unige.it</nlm:affiliation>
<country xml:lang="fr">Italie</country>
<wicri:regionArea>Department of Chemical and Process Engineering, University of Genoa, via Opera Pia 15, 16145 Genoa</wicri:regionArea>
<wicri:noRegion>16145 Genoa</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Gandolfi, R" sort="Gandolfi, R" uniqKey="Gandolfi R" first="R" last="Gandolfi">R. Gandolfi</name>
</author>
<author><name sortKey="Zilli, M" sort="Zilli, M" uniqKey="Zilli M" first="M" last="Zilli">M. Zilli</name>
</author>
<author><name sortKey="Molinari, F" sort="Molinari, F" uniqKey="Molinari F" first="F" last="Molinari">F. Molinari</name>
</author>
<author><name sortKey="Binaghi, L" sort="Binaghi, L" uniqKey="Binaghi L" first="L" last="Binaghi">L. Binaghi</name>
</author>
<author><name sortKey="Perego, P" sort="Perego, P" uniqKey="Perego P" first="P" last="Perego">P. Perego</name>
</author>
<author><name sortKey="Del Borghi, M" sort="Del Borghi, M" uniqKey="Del Borghi M" first="M" last="Del Borghi">M. Del Borghi</name>
</author>
</analytic>
<series><title level="j">Applied microbiology and biotechnology</title>
<idno type="ISSN">0175-7598</idno>
<imprint><date when="2005" type="published">2005</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Acylation</term>
<term>Aspergillus oryzae (metabolism)</term>
<term>Biotransformation</term>
<term>Carboxylesterase (metabolism)</term>
<term>Catalysis</term>
<term>Ethanol (metabolism)</term>
<term>Freeze Drying</term>
<term>Kinetics</term>
<term>Mycelium (metabolism)</term>
<term>Phenylacetates (metabolism)</term>
<term>Rhizopus (metabolism)</term>
<term>Temperature</term>
<term>Thermodynamics</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Carboxylesterase</term>
<term>Ethanol</term>
<term>Phenylacetates</term>
</keywords>
<keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Aspergillus oryzae</term>
<term>Mycelium</term>
<term>Rhizopus</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Acylation</term>
<term>Biotransformation</term>
<term>Catalysis</term>
<term>Freeze Drying</term>
<term>Kinetics</term>
<term>Temperature</term>
<term>Thermodynamics</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Lyophilized mycelia of Aspergillus oryzae CBS 102.07, Aspergillus oryzae MIM, Rhizopus oryzae CBS 112.07, Rhizopus oryzae CBS 391.34, Rhizopus oryzae CBS 260.28 and Rhizopus oryzae CBS 328.47 were tested in this study to select the best biocatalysts for ethanol acylation with phenylacetic acid. The mycelium-bound carboxylesterase activity of A. oryzae MIM, which exhibited the best performances, was initially investigated at 50 degrees C, either in 0.1 M phosphate buffer or in n-heptane to catalyse the hydrolysis or the synthesis, respectively, of ethyl phenylacetate. The results in terms of product and substrate concentrations versus time were used to estimate the maximum molar conversions at equilibrium, the equilibrium constants, and the times needed to reach half maximum conversions, thus providing sufficient information about this biotransformation. The values of the apparent equilibrium constants, estimated at 20 degrees C</div>
</front>
</TEI>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Musique/explor/OperaV1/Data/Main/Curation
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001A00 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Curation/biblio.hfd -nk 001A00 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Wicri/Musique |area= OperaV1 |flux= Main |étape= Curation |type= RBID |clé= pubmed:15650849 |texte= Synthesis of ethyl phenylacetate by lyophilized mycelium of Aspergillus oryzae. }}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Curation/RBID.i -Sk "pubmed:15650849" \ | HfdSelect -Kh $EXPLOR_AREA/Data/Main/Curation/biblio.hfd \ | NlmPubMed2Wicri -a OperaV1
This area was generated with Dilib version V0.6.21. |