Nanofunctionalisation for the treatment of peripheral nervous system injuries.
Identifieur interne : 001804 ( Main/Curation ); précédent : 001803; suivant : 001805Nanofunctionalisation for the treatment of peripheral nervous system injuries.
Auteurs : L. Pastorino [Italie] ; Federico Caneva Soumetz ; C. RuggieroSource :
- IEE proceedings. Nanobiotechnology [ 1478-1581 ] ; 2006.
English descriptors
- KwdEn :
- Animals, Antibodies, Monoclonal (administration & dosage), Antibodies, Monoclonal (chemistry), Antibodies, Monoclonal (immunology), Biocompatible Materials (chemistry), Drug Delivery Systems (methods), Humans, Hyaluronic Acid (analogs & derivatives), Hyaluronic Acid (chemistry), Materials Testing, Nanostructures (chemistry), Nanostructures (ultrastructure), Nanotechnology (methods), Nerve Regeneration (drug effects), Peripheral Nervous System (injuries), Receptors, Tumor Necrosis Factor (immunology), Receptors, Tumor Necrosis Factor, Type I, Tissue Engineering (methods), Tumor Necrosis Factor Decoy Receptors.
- MESH :
- chemical , administration & dosage : Antibodies, Monoclonal.
- chemical , analogs & derivatives : Hyaluronic Acid.
- chemical , chemistry : Antibodies, Monoclonal, Biocompatible Materials, Hyaluronic Acid.
- chemical , immunology : Antibodies, Monoclonal, Receptors, Tumor Necrosis Factor.
- chemistry : Nanostructures.
- drug effects : Nerve Regeneration.
- injuries : Peripheral Nervous System.
- methods : Drug Delivery Systems, Nanotechnology, Tissue Engineering.
- ultrastructure : Nanostructures.
- Animals, Humans, Materials Testing, Receptors, Tumor Necrosis Factor, Type I, Tumor Necrosis Factor Decoy Receptors.
Abstract
A construct based on the electrostatic layer-by-layer self assembly technique has been fabricated, to be used as a tailored device to encourage nerve regeneration. A multilayered nanocoating composed of three precursor bilayers of cationic poly(dimethyldiallylammonium) chloride (PDDA) and anionic poly(styrenesulfonate) (PSS), followed by bilayers of poly-D-lysine (PDL) and antibody specific to transforming growth factor 1 (anti-TGF-1), has been deposited on HYAFF 11. The assembly process has been monitored by quartz crystal microbalance (QCM) for its characterisation and then it has been used on HYAFF 11. Structural studies of the resulting multilayers confirmed stepwise deposition of anti-TGF-1, with an average layer thickness of 2.2+/-0.2 nm and an average surface density of 0.36+/-0.03 mug cm(-2). Scanning electron microscopy has been used to characterise multilayer uniformity. Finally, the immunological activity of the multilayered structure has been assessed. The results show that anti-TGF-1 can be included in its active form in a predetermined multilayered structure onto HYAFF 11 with quantitative control of layer thickness and weight, providing a high tool with great potential in tissue engineering.
DOI: 10.1049/ip-nbt:20050030
PubMed: 16671819
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pubmed:16671819Le document en format XML
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Pastorino</name><affiliation wicri:level="1"><nlm:affiliation>Department of Communication, Computer & System Sciences, University of Genoa, Via Opera Pia 13, 16145 Genoa, Italy.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>Department of Communication, Computer & System Sciences, University of Genoa, Via Opera Pia 13, 16145 Genoa</wicri:regionArea><wicri:noRegion>16145 Genoa</wicri:noRegion></affiliation></author><author><name sortKey="Soumetz, Federico Caneva" sort="Soumetz, Federico Caneva" uniqKey="Soumetz F" first="Federico Caneva" last="Soumetz">Federico Caneva Soumetz</name></author><author><name sortKey="Ruggiero, C" sort="Ruggiero, C" uniqKey="Ruggiero C" first="C" last="Ruggiero">C. Ruggiero</name></author></analytic><series><title level="j">IEE proceedings. Nanobiotechnology</title><idno type="ISSN">1478-1581</idno><imprint><date when="2006" type="published">2006</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Antibodies, Monoclonal (administration & dosage)</term><term>Antibodies, Monoclonal (chemistry)</term><term>Antibodies, Monoclonal (immunology)</term><term>Biocompatible Materials (chemistry)</term><term>Drug Delivery Systems (methods)</term><term>Humans</term><term>Hyaluronic Acid (analogs & derivatives)</term><term>Hyaluronic Acid (chemistry)</term><term>Materials Testing</term><term>Nanostructures (chemistry)</term><term>Nanostructures (ultrastructure)</term><term>Nanotechnology (methods)</term><term>Nerve Regeneration (drug effects)</term><term>Peripheral Nervous System (injuries)</term><term>Receptors, Tumor Necrosis Factor (immunology)</term><term>Receptors, Tumor Necrosis Factor, Type I</term><term>Tissue Engineering (methods)</term><term>Tumor Necrosis Factor Decoy Receptors</term></keywords><keywords scheme="MESH" type="chemical" qualifier="administration & dosage" xml:lang="en"><term>Antibodies, Monoclonal</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analogs & derivatives" xml:lang="en"><term>Hyaluronic Acid</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Antibodies, Monoclonal</term><term>Biocompatible Materials</term><term>Hyaluronic Acid</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Antibodies, Monoclonal</term><term>Receptors, Tumor Necrosis Factor</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Nanostructures</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Nerve Regeneration</term></keywords><keywords scheme="MESH" qualifier="injuries" xml:lang="en"><term>Peripheral Nervous System</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Drug Delivery Systems</term><term>Nanotechnology</term><term>Tissue Engineering</term></keywords><keywords scheme="MESH" qualifier="ultrastructure" xml:lang="en"><term>Nanostructures</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Humans</term><term>Materials Testing</term><term>Receptors, Tumor Necrosis Factor, Type I</term><term>Tumor Necrosis Factor Decoy Receptors</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">A construct based on the electrostatic layer-by-layer self assembly technique has been fabricated, to be used as a tailored device to encourage nerve regeneration. A multilayered nanocoating composed of three precursor bilayers of cationic poly(dimethyldiallylammonium) chloride (PDDA) and anionic poly(styrenesulfonate) (PSS), followed by bilayers of poly-D-lysine (PDL) and antibody specific to transforming growth factor 1 (anti-TGF-1), has been deposited on HYAFF 11. The assembly process has been monitored by quartz crystal microbalance (QCM) for its characterisation and then it has been used on HYAFF 11. Structural studies of the resulting multilayers confirmed stepwise deposition of anti-TGF-1, with an average layer thickness of 2.2+/-0.2 nm and an average surface density of 0.36+/-0.03 mug cm(-2). Scanning electron microscopy has been used to characterise multilayer uniformity. Finally, the immunological activity of the multilayered structure has been assessed. The results show that anti-TGF-1 can be included in its active form in a predetermined multilayered structure onto HYAFF 11 with quantitative control of layer thickness and weight, providing a high tool with great potential in tissue engineering.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
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