Immunolabelling of Atrazine Residues in Soil
Identifieur interne : 000397 ( Istex/Corpus ); précédent : 000396; suivant : 000398Immunolabelling of Atrazine Residues in Soil
Auteurs : Alexander Hahn ; Fritz Frimmel ; Albert Haisch ; Günter Henkelmann ; Bertold HockSource :
- Zeitschrift für Pflanzenernährung und Bodenkunde [ 0044-3263 ] ; 1992.
Abstract
An immunolabelling procedure was developed to detect bound pesticide residues in soil using atrazine as an example. Polyclonal antibodies directed against atrazine were raised in rabbits. Antigen‐binding fragments (Fab) were prepared and coupled to the fluorescent stain Rose Bengal B. The dye conjugate produced a fluorescence signal which was related to the amount of atrazine found in native soil samples. Preincubation with unlabelled immunoglobulines (IgG) from preimmune sera proved to be essential for blocking unspecific binding of the antibodies which varied greatly between different samples of soil material. Labelled antibodies, whose specific binding sites had been blocked by free atrazine, were used as controls. Strong unspecific labelling, which also could be suppressed with IgG from preimmune sera, was observed in a soil under a barn. Changes due to the permanent dryness and the lack of aeration are discussed as possible causes.
Url:
DOI: 10.1002/jpln.19921550308
Links to Exploration step
ISTEX:4B2670A3AEF29602DE08ED3E60401B262A5EAD28Le document en format XML
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Antigen‐binding fragments (Fab) were prepared and coupled to the fluorescent stain Rose Bengal B. The dye conjugate produced a fluorescence signal which was related to the amount of atrazine found in native soil samples. Preincubation with unlabelled immunoglobulines (IgG) from preimmune sera proved to be essential for blocking unspecific binding of the antibodies which varied greatly between different samples of soil material. Labelled antibodies, whose specific binding sites had been blocked by free atrazine, were used as controls. Strong unspecific labelling, which also could be suppressed with IgG from preimmune sera, was observed in a soil under a barn. Changes due to the permanent dryness and the lack of aeration are discussed as possible causes.</p></abstract><abstract xml:lang="de"><p>Immunmarkierung von Atrazinrückständen im Boden Zur Erfassung gebundener Rückstände von Pflanzenbehandlungsmitteln im Boden wurde am Beispiel des Atrazins ein Immunmarkierungsverfahren entwickelt. Polyklonale Antikörper gegen Atrazin wurden in Kaninchen gewonnen. Daran schloß sich die Kopplung der antigenbindenden Fragmente (Fab) an den Fluoreszenzfarbstoff Bengalrosa an. Das Farbstoffkonjugat lieferte ein Fluoreszenzsignal, das bei nativen Böden mit der Menge an gebundenem Atrazin korreliert war. Zur Blockierung unspezifischer Antikörperbindungen, die zwischen den einzelnen Bodenproben stark schwankten, erwies sich eine Vorinkubation mit unmarkierten Präimmunseren als essentiell. Als Nullkontrollen dienten markierte Antikörper, deren spezifische Bindungsstellen durch freies Atrazin blokkiert waren. Starke unspezifische Bindungen, die ebenfalls durch Inkubation mit Präimmunseren beseitigt werden konnten, wurden bei einem unter einer Scheune entnommenen Boden beobachtet. Als Ursache werden Veränderungen durch permanente Trockenheit und Luftabschluß diskutiert.</p></abstract><textClass><keywords scheme="Journal Subject"><list><head>article category</head><item><term>Article</term></item></list></keywords></textClass></profileDesc><revisionDesc><change when="1991-12-16">Registration</change><change when="1992">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><original>false</original><mimetype>text/plain</mimetype><extension>txt</extension><uri>https://api.istex.fr/document/4B2670A3AEF29602DE08ED3E60401B262A5EAD28/fulltext/txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Wiley, elements deleted: body"><istex:xmlDeclaration>version="1.0" encoding="UTF-8" standalone="yes"</istex:xmlDeclaration><istex:document><component version="2.0" type="serialArticle" xml:lang="en"><header><publicationMeta level="product"><publisherInfo><publisherName>WILEY‐VCH Verlag</publisherName><publisherLoc>Weinheim</publisherLoc></publisherInfo><doi registered="yes">10.1002/(ISSN)1522-2624d</doi><issn type="print">0044-3263</issn><issn type="electronic">1522-2624</issn><idGroup><id type="product" value="JPLN"></id></idGroup><titleGroup><title type="main" xml:lang="de" sort="ZEITSCHRIFT FUR PFLANZENERNAHRUNG UND BODENKUNDE">Zeitschrift für Pflanzenernährung und Bodenkunde</title><title type="short">Z. 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Landesanstalt für Bodenkultur und Pflanzenbau, Menzinger Str. 54, W‐8000 München 19, FRG</unparsedAffiliation></affiliation></affiliationGroup><abstractGroup><abstract type="main" xml:lang="en"><title type="main">Abstract</title><p>An immunolabelling procedure was developed to detect bound pesticide residues in soil using atrazine as an example. Polyclonal antibodies directed against atrazine were raised in rabbits. Antigen‐binding fragments (F<sub>ab</sub>) were prepared and coupled to the fluorescent stain Rose Bengal B. The dye conjugate produced a fluorescence signal which was related to the amount of atrazine found in native soil samples. Preincubation with unlabelled immunoglobulines (IgG) from preimmune sera proved to be essential for blocking unspecific binding of the antibodies which varied greatly between different samples of soil material. Labelled antibodies, whose specific binding sites had been blocked by free atrazine, were used as controls. Strong unspecific labelling, which also could be suppressed with IgG from preimmune sera, was observed in a soil under a barn. Changes due to the permanent dryness and the lack of aeration are discussed as possible causes.</p></abstract><abstract type="main" xml:lang="de"><p><b>Immunmarkierung von Atrazinrückständen im Boden</b></p><p>Zur Erfassung gebundener Rückstände von Pflanzenbehandlungsmitteln im Boden wurde am Beispiel des Atrazins ein Immunmarkierungsverfahren entwickelt. Polyklonale Antikörper gegen Atrazin wurden in Kaninchen gewonnen. Daran schloß sich die Kopplung der antigenbindenden Fragmente (F<sub>ab</sub>) an den Fluoreszenzfarbstoff Bengalrosa an. Das Farbstoffkonjugat lieferte ein Fluoreszenzsignal, das bei nativen Böden mit der Menge an gebundenem Atrazin korreliert war. Zur Blockierung unspezifischer Antikörperbindungen, die zwischen den einzelnen Bodenproben stark schwankten, erwies sich eine Vorinkubation mit unmarkierten Präimmunseren als essentiell. Als Nullkontrollen dienten markierte Antikörper, deren spezifische Bindungsstellen durch freies Atrazin blokkiert waren. Starke unspezifische Bindungen, die ebenfalls durch Inkubation mit Präimmunseren beseitigt werden konnten, wurden bei einem unter einer Scheune entnommenen Boden beobachtet. Als Ursache werden Veränderungen durch permanente Trockenheit und Luftabschluß diskutiert.</p></abstract></abstractGroup></contentMeta></header></component></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Immunolabelling of Atrazine Residues in Soil</title></titleInfo><titleInfo type="alternative" contentType="CDATA" lang="en"><title>Immunolabelling of Atrazine Residues in Soil</title></titleInfo><name type="personal"><namePart type="given">Alexander</namePart><namePart type="family">Hahn</namePart><affiliation>Lehrstuhl für Botanik der TU München (Weihenstephan), W‐8050 Freising 12, FRG</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Fritz</namePart><namePart type="family">Frimmel</namePart><affiliation>Lehrstuhl für Wasserchemie der Universität Karlsruhe, Richard‐Willstätter‐Allee 5, W‐7500 Karlsruhe 1, FRG</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Albert</namePart><namePart type="family">Haisch</namePart><affiliation>Bayer. Landesanstalt für Bodenkultur und Pflanzenbau, Menzinger Str. 54, W‐8000 München 19, FRG</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Günter</namePart><namePart type="family">Henkelmann</namePart><affiliation>Bayer. Landesanstalt für Bodenkultur und Pflanzenbau, Menzinger Str. 54, W‐8000 München 19, FRG</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Bertold</namePart><namePart type="family">Hock</namePart><affiliation>Lehrstuhl für Botanik der TU München (Weihenstephan), W‐8050 Freising 12, FRG</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="article" displayLabel="article"></genre><originInfo><publisher>WILEY‐VCH Verlag</publisher><place><placeTerm type="text">Weinheim</placeTerm></place><dateIssued encoding="w3cdtf">1992</dateIssued><dateValid encoding="w3cdtf">1991-12-16</dateValid><copyrightDate encoding="w3cdtf">1992</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><physicalDescription><internetMediaType>text/html</internetMediaType><extent unit="figures">5</extent><extent unit="references">11</extent></physicalDescription><abstract lang="en">An immunolabelling procedure was developed to detect bound pesticide residues in soil using atrazine as an example. Polyclonal antibodies directed against atrazine were raised in rabbits. Antigen‐binding fragments (Fab) were prepared and coupled to the fluorescent stain Rose Bengal B. The dye conjugate produced a fluorescence signal which was related to the amount of atrazine found in native soil samples. Preincubation with unlabelled immunoglobulines (IgG) from preimmune sera proved to be essential for blocking unspecific binding of the antibodies which varied greatly between different samples of soil material. Labelled antibodies, whose specific binding sites had been blocked by free atrazine, were used as controls. Strong unspecific labelling, which also could be suppressed with IgG from preimmune sera, was observed in a soil under a barn. Changes due to the permanent dryness and the lack of aeration are discussed as possible causes.</abstract><abstract lang="de">Immunmarkierung von Atrazinrückständen im Boden Zur Erfassung gebundener Rückstände von Pflanzenbehandlungsmitteln im Boden wurde am Beispiel des Atrazins ein Immunmarkierungsverfahren entwickelt. Polyklonale Antikörper gegen Atrazin wurden in Kaninchen gewonnen. Daran schloß sich die Kopplung der antigenbindenden Fragmente (Fab) an den Fluoreszenzfarbstoff Bengalrosa an. Das Farbstoffkonjugat lieferte ein Fluoreszenzsignal, das bei nativen Böden mit der Menge an gebundenem Atrazin korreliert war. Zur Blockierung unspezifischer Antikörperbindungen, die zwischen den einzelnen Bodenproben stark schwankten, erwies sich eine Vorinkubation mit unmarkierten Präimmunseren als essentiell. Als Nullkontrollen dienten markierte Antikörper, deren spezifische Bindungsstellen durch freies Atrazin blokkiert waren. Starke unspezifische Bindungen, die ebenfalls durch Inkubation mit Präimmunseren beseitigt werden konnten, wurden bei einem unter einer Scheune entnommenen Boden beobachtet. Als Ursache werden Veränderungen durch permanente Trockenheit und Luftabschluß diskutiert.</abstract><relatedItem type="host"><titleInfo><title>Zeitschrift für Pflanzenernährung und Bodenkunde</title></titleInfo><titleInfo type="abbreviated"><title>Z. Pflanzenernaehr. Bodenk.</title></titleInfo><genre type="Journal">journal</genre><subject><genre>article category</genre><topic>Article</topic></subject><identifier type="ISSN">0044-3263</identifier><identifier type="eISSN">1522-2624</identifier><identifier type="DOI">10.1002/(ISSN)1522-2624d</identifier><identifier type="PublisherID">JPLN</identifier><part><date>1992</date><detail type="volume"><caption>vol.</caption><number>155</number></detail><detail type="issue"><caption>no.</caption><number>3</number></detail><extent unit="pages"><start>203</start><end>208</end><total>6</total></extent></part></relatedItem><identifier type="istex">4B2670A3AEF29602DE08ED3E60401B262A5EAD28</identifier><identifier type="DOI">10.1002/jpln.19921550308</identifier><identifier type="ArticleID">JPLN19921550308</identifier><accessCondition type="use and reproduction" contentType="copyright">Copyright © 1992 WILEY‐VCH Verlag GmbH & Co. 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