Ultrastructural alterations in macrophages after phagocytosis of acrylic microspheres
Identifieur interne : 000392 ( Istex/Corpus ); précédent : 000391; suivant : 000393Ultrastructural alterations in macrophages after phagocytosis of acrylic microspheres
Auteurs : Peter Edman ; Ingvar Sjöholm ; Ulf BrunkSource :
- Journal of Pharmaceutical Sciences [ 0022-3549 ] ; 1984-02.
English descriptors
- KwdEn :
- Macrophages, peritoneal—cultured from mice, effect of phagocytosis of microparticles, ultrastructural cellular alterations, Microspheres—polyacrylamide, phagocytosis by cultured mouse peritoneal macrophages, ultrastructural cellular alterations, Phagocytosis—microparticles, by cultured mouse peritoneal macrophages, ultrastructural cellular alterations.
Abstract
The effect of microparticles on the survival of cultured mouse peritoneal macrophages was investigated using doses of 0.01‐0.1 mg of lyophilized particles/ml of medium and 5 ± 105 cells, corresponding to ∼4000–40,000 particles per cell. The lowest dose did not significantly change the survival time as compared with the controls, while ∼75% of the cells were lost during the first 48 h on exposure to the highest dose. High doses of particles induce cellular damage. The morphology and stability of the lysosomal apparatus was followed with electron microscopy, acid phosphatase cytochemistry, and acridine orange uptake. Alteration of the lysosomal vacuome was characterized by a greatly enhanced rate of autophagocytosis, the formation of huge secondary lysosomes containing microparticles, and labilization of the vacuome with loss of acidity and a tendency to leak acid phosphatase into the cell sap.
Url:
DOI: 10.1002/jps.2600730204
Links to Exploration step
ISTEX:0E28DE5B6C9B6FD6D1A9FA60E9306E5BDC22ACB4Le document en format XML
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The lowest dose did not significantly change the survival time as compared with the controls, while ∼75% of the cells were lost during the first 48 h on exposure to the highest dose. High doses of particles induce cellular damage. The morphology and stability of the lysosomal apparatus was followed with electron microscopy, acid phosphatase cytochemistry, and acridine orange uptake. Alteration of the lysosomal vacuome was characterized by a greatly enhanced rate of autophagocytosis, the formation of huge secondary lysosomes containing microparticles, and labilization of the vacuome with loss of acidity and a tendency to leak acid phosphatase into the cell sap.</div></front></TEI><istex><corpusName>wiley</corpusName><author><json:item><name>Peter Edman</name><affiliations><json:string>Department of Pharmaceutical Biochemistry, Biomedicum, S‐751 23 Uppsala, Sweden</json:string><json:string>Current Address: National Board of Health and Welfare, Department of Drugs, Division of Pharmacy, S‐751 25 Uppsala, Sweden</json:string></affiliations></json:item><json:item><name>Ingvar Sjöholm</name><affiliations><json:string>Department of Pharmaceutical Biochemistry, Biomedicum, S‐751 23 Uppsala, Sweden</json:string><json:string>Current Address: National Board of Health and Welfare, Department of Drugs, Division of Pharmacy, S‐751 25 Uppsala, Sweden</json:string></affiliations></json:item><json:item><name>Ulf Brunk</name><affiliations><json:string>Linköping University, Department of Pathology, University Hospital, S‐581 85 Linköping, Sweden</json:string></affiliations></json:item></author><subject><json:item><lang><json:string>eng</json:string></lang><value>Microspheres—polyacrylamide, phagocytosis by cultured mouse peritoneal macrophages, ultrastructural cellular alterations</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Macrophages, peritoneal—cultured from mice, effect of phagocytosis of microparticles, ultrastructural cellular alterations</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Phagocytosis—microparticles, by cultured mouse peritoneal macrophages, ultrastructural cellular alterations</value></json:item></subject><articleId><json:string>JPS2600730204</json:string></articleId><language><json:string>eng</json:string></language><abstract>The effect of microparticles on the survival of cultured mouse peritoneal macrophages was investigated using doses of 0.01‐0.1 mg of lyophilized particles/ml of medium and 5 ± 105 cells, corresponding to ∼4000–40,000 particles per cell. 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The lowest dose did not significantly change the survival time as compared with the controls, while ∼75% of the cells were lost during the first 48 h on exposure to the highest dose. High doses of particles induce cellular damage. The morphology and stability of the lysosomal apparatus was followed with electron microscopy, acid phosphatase cytochemistry, and acridine orange uptake. Alteration of the lysosomal vacuome was characterized by a greatly enhanced rate of autophagocytosis, the formation of huge secondary lysosomes containing microparticles, and labilization of the vacuome with loss of acidity and a tendency to leak acid phosphatase into the cell sap.</p></abstract></abstractGroup></contentMeta></header></component></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Ultrastructural alterations in macrophages after phagocytosis of acrylic microspheres</title></titleInfo><titleInfo type="abbreviated" lang="en"><title>Ultrastructural Alterations In Macrophages After Phagocytosis Of Acrylic Microspheres</title></titleInfo><titleInfo type="alternative" contentType="CDATA" lang="en"><title>Ultrastructural alterations in macrophages after phagocytosis of acrylic microspheres</title></titleInfo><name type="personal"><namePart type="given">Peter</namePart><namePart type="family">Edman</namePart><affiliation>Department of Pharmaceutical Biochemistry, Biomedicum, S‐751 23 Uppsala, Sweden</affiliation><affiliation>Current Address: National Board of Health and Welfare, Department of Drugs, Division of Pharmacy, S‐751 25 Uppsala, Sweden</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Ingvar</namePart><namePart type="family">Sjöholm</namePart><affiliation>Department of Pharmaceutical Biochemistry, Biomedicum, S‐751 23 Uppsala, Sweden</affiliation><affiliation>Current Address: National Board of Health and Welfare, Department of Drugs, Division of Pharmacy, S‐751 25 Uppsala, Sweden</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Ulf</namePart><namePart type="family">Brunk</namePart><affiliation>Linköping University, Department of Pathology, University Hospital, S‐581 85 Linköping, Sweden</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="article" displayLabel="article"></genre><originInfo><publisher>Wiley Subscription Services, Inc., A Wiley Company</publisher><place><placeTerm type="text">Washington</placeTerm></place><dateIssued encoding="w3cdtf">1984-02</dateIssued><dateCaptured encoding="w3cdtf">1982-06-15</dateCaptured><dateValid encoding="w3cdtf">1982-12-08</dateValid><copyrightDate encoding="w3cdtf">1984</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><physicalDescription><internetMediaType>text/html</internetMediaType><extent unit="figures">7</extent><extent unit="tables">1</extent><extent unit="references">19</extent></physicalDescription><abstract lang="en">The effect of microparticles on the survival of cultured mouse peritoneal macrophages was investigated using doses of 0.01‐0.1 mg of lyophilized particles/ml of medium and 5 ± 105 cells, corresponding to ∼4000–40,000 particles per cell. The lowest dose did not significantly change the survival time as compared with the controls, while ∼75% of the cells were lost during the first 48 h on exposure to the highest dose. High doses of particles induce cellular damage. The morphology and stability of the lysosomal apparatus was followed with electron microscopy, acid phosphatase cytochemistry, and acridine orange uptake. Alteration of the lysosomal vacuome was characterized by a greatly enhanced rate of autophagocytosis, the formation of huge secondary lysosomes containing microparticles, and labilization of the vacuome with loss of acidity and a tendency to leak acid phosphatase into the cell sap.</abstract><subject lang="en"><genre>Keywords</genre><topic>Microspheres—polyacrylamide, phagocytosis by cultured mouse peritoneal macrophages, ultrastructural cellular alterations</topic><topic>Macrophages, peritoneal—cultured from mice, effect of phagocytosis of microparticles, ultrastructural cellular alterations</topic><topic>Phagocytosis—microparticles, by cultured mouse peritoneal macrophages, ultrastructural cellular alterations</topic></subject><relatedItem type="host"><titleInfo><title>Journal of Pharmaceutical Sciences</title></titleInfo><titleInfo type="abbreviated"><title>J. Pharm. 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