Genome size and chromosomes in marine sponges [Suberites domuncula, Geodia cydonium]
Identifieur interne : 000342 ( Istex/Corpus ); précédent : 000341; suivant : 000343Genome size and chromosomes in marine sponges [Suberites domuncula, Geodia cydonium]
Auteurs : Georg Imsiecke ; Marcio Custodio ; Radovan Borojevic ; Renate Steffen ; Mohamed A. Moustafa ; Werner E. G. MüllerSource :
- Cell Biology International [ 1065-6995 ] ; 1995-12.
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Abstract
The genome size of the marine sponges Suberites domuncula and Geodia cydonium has been determined by flow cytofluorometric analysis using diamidino‐phenylindole [DAPI]. Using human lymphocytes as reference the amount of DNA in cells from S. domuncula has been determined to be 3.7 pg and that of G. cydonium 3.3 pg. While no chromosomes could be identified in G. cydonium, the karyotype of the Suberites domuncula is 32 chromsomes in the diploid state. The size of the chromosomes was between 0.25 and 1.0 μm. No pronounced banding pattern was visible.
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DOI: 10.1006/cbir.1995.1041
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ISTEX:A6461004F38023328955FF2B061F6FCDA19E14C0Le document en format XML
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Using human lymphocytes as reference the amount of DNA in cells from S. domuncula has been determined to be 3.7 pg and that of G. cydonium 3.3 pg. While no chromosomes could be identified in G. cydonium, the karyotype of the Suberites domuncula is 32 chromsomes in the diploid state. The size of the chromosomes was between 0.25 and 1.0 μm. 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G.</givenNames><familyName>Müller</familyName></personName></creator></creators><affiliationGroup><affiliation xml:id="a1" countryCode="DE"><unparsedAffiliation>Institut für Physiologische Chemie, Universität, Duesbergweg 6, D‐55099 Mainz, Germany</unparsedAffiliation></affiliation><affiliation xml:id="a2" countryCode="BR"><unparsedAffiliation>Departamento de Bioquímica, Instituto de Química, Universidade Federal do Rio de Janeiro, Caixa Postal 68021, 21944‐970 Rio De Janeiro, Brazil</unparsedAffiliation></affiliation><affiliation xml:id="a3" countryCode="DE"><unparsedAffiliation>Institut für Molekulargenetik, Universität, Becherweg 32, D‐55099 Mainz, Germany</unparsedAffiliation></affiliation></affiliationGroup><keywordGroup xml:lang="en"><keyword xml:id="k1">Sponges</keyword><keyword xml:id="k2"><i>Suberites domuncula</i></keyword><keyword xml:id="k3">karyotype</keyword><keyword xml:id="k4">genome size</keyword></keywordGroup><abstractGroup><abstract type="main" xml:lang="en"><title type="main">Abstract</title><p>The genome size of the marine sponges <i>Suberites domuncula</i> and <i>Geodia cydonium</i> has been determined by flow cytofluorometric analysis using diamidino‐phenylindole [DAPI]. Using human lymphocytes as reference the amount of DNA in cells from <i>S. domuncula</i> has been determined to be 3.7 pg and that of <i>G. cydonium</i> 3.3 pg. While no chromosomes could be identified in <i>G. cydonium</i>, the karyotype of the <i>Suberites domuncula</i> is 32 chromsomes in the diploid state. The size of the chromosomes was between 0.25 and 1.0 μm. No pronounced banding pattern was visible.</p></abstract></abstractGroup></contentMeta></header></component></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Genome size and chromosomes in marine sponges [Suberites domuncula, Geodia cydonium]</title></titleInfo><titleInfo type="alternative" contentType="CDATA" lang="en"><title>Genome size and chromosomes in marine sponges [</title></titleInfo><name type="personal"><namePart type="given">Georg</namePart><namePart type="family">Imsiecke</namePart><affiliation>Institut für Physiologische Chemie, Universität, Duesbergweg 6, D‐55099 Mainz, Germany</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Marcio</namePart><namePart type="family">Custodio</namePart><affiliation>Departamento de Bioquímica, Instituto de Química, Universidade Federal do Rio de Janeiro, Caixa Postal 68021, 21944‐970 Rio De Janeiro, Brazil</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Radovan</namePart><namePart type="family">Borojevic</namePart><affiliation>Departamento de Bioquímica, Instituto de Química, Universidade Federal do Rio de Janeiro, Caixa Postal 68021, 21944‐970 Rio De Janeiro, Brazil</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Renate</namePart><namePart type="family">Steffen</namePart><affiliation>Institut für Physiologische Chemie, Universität, Duesbergweg 6, D‐55099 Mainz, Germany</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Mohamed A.</namePart><namePart type="family">Moustafa</namePart><affiliation>Institut für Molekulargenetik, Universität, Becherweg 32, D‐55099 Mainz, Germany</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Werner E. G.</namePart><namePart type="family">Müller</namePart><affiliation>Institut für Physiologische Chemie, Universität, Duesbergweg 6, D‐55099 Mainz, Germany</affiliation><description>Correspondence: Institut für Physiologische Chemie, Universität, Duesbergweg 6, D‐55099 Mainz, Germany</description><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="article" displayLabel="article"></genre><originInfo><publisher>Blackwell Publishing Ltd</publisher><place><placeTerm type="text">Oxford, UK</placeTerm></place><dateIssued encoding="w3cdtf">1995-12</dateIssued><copyrightDate encoding="w3cdtf">1995</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><physicalDescription><internetMediaType>text/html</internetMediaType></physicalDescription><abstract lang="en">The genome size of the marine sponges Suberites domuncula and Geodia cydonium has been determined by flow cytofluorometric analysis using diamidino‐phenylindole [DAPI]. Using human lymphocytes as reference the amount of DNA in cells from S. domuncula has been determined to be 3.7 pg and that of G. cydonium 3.3 pg. While no chromosomes could be identified in G. cydonium, the karyotype of the Suberites domuncula is 32 chromsomes in the diploid state. The size of the chromosomes was between 0.25 and 1.0 μm. No pronounced banding pattern was visible.</abstract><subject lang="en"><genre>Keywords</genre><topic>Sponges</topic><topic>Suberites domuncula</topic><topic>karyotype</topic><topic>genome size</topic></subject><relatedItem type="host"><titleInfo><title>Cell Biology International</title></titleInfo><genre type="Journal">journal</genre><identifier type="ISSN">1065-6995</identifier><identifier type="eISSN">1095-8355</identifier><identifier type="DOI">10.1002/(ISSN)1095-8355</identifier><identifier type="PublisherID">CBIN</identifier><part><date>1995</date><detail type="volume"><caption>vol.</caption><number>19</number></detail><detail type="issue"><caption>no.</caption><number>12</number></detail><extent unit="pages"><start>995</start><end>1000</end><total>6</total></extent></part></relatedItem><identifier type="istex">A6461004F38023328955FF2B061F6FCDA19E14C0</identifier><identifier type="DOI">10.1006/cbir.1995.1041</identifier><identifier type="ArticleID">CBIN328</identifier><accessCondition type="use and reproduction" contentType="copyright">© The Author(s) Journal compilation © 1995 International Federation for Cell Biology</accessCondition><recordInfo><recordContentSource>WILEY</recordContentSource><recordOrigin>Blackwell Publishing Ltd</recordOrigin></recordInfo></mods></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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