Intracellular accumulation of incompletely processed transforming growth factor-alpha polypeptides in ground glass hepatocytes of chronic hepatitis B virus infection
Identifieur interne : 000213 ( Istex/Corpus ); précédent : 000212; suivant : 000214Intracellular accumulation of incompletely processed transforming growth factor-alpha polypeptides in ground glass hepatocytes of chronic hepatitis B virus infection
Auteurs : Peter Schirmacher ; Dagmar Schau ; Hans Peter DienesSource :
- Journal of Hepatology [ 0168-8278 ] ; 1995.
Abstract
Background: Transforming growth factor-alpha is an intracellularly processed and secreted polypeptide that induces a proliferative response in epithelial target cells and represents a potential regulatory factor in embryonic development, liver regeneration, and also hepatocarcinogenesis. We have observed focal transforming growth factor-alpha expression in liver tissues with chronic hepatitis B virus infection. Methods: To further elucidate the nature of this focal transforming growth factor-alpha accumulation were have analyzed overall 23 different liver tissues with chronic hepatitis B virus and hepatitis C virus infection as well as normal liver tissues by immunohistology, ELISA, and Western immunoblot with and without immunoprecipitation. Results: By immunohistology transforming growth factor-alpha polypeptides showed focal subcellular accumulation in ground glass hepatocytes, the histological hallmark of chronic hepatitis B virus infection, in co-localization with HBV-preS1 antigen. By ELISA and Western immunoblot increased tissue concentrations of transforming growth factor-alpha were demonstrated in chronically hepatitis B virus-infected liver tissues with ground glass hepatocytes, especially a 15-kD polypeptide, most likely representing an incompletely processed transforming growth factor-alpha polypeptide. transforming growth factor-alpha retention in ground glass hepatocytes is not a general unspecific effect, since it was not observed for several other secretory liver proteins. Accumulated transforming growth factor-alpha in ground glass hepatocytes does not co-localize with Epidermal Growth Factor Receptor expression. Conclusion: Thus evidence is presented that a principally secreted (viral) polypeptide (HBV-preS1) can interfere with the secretion and processing of a second (cellular) protein (transforming growth factor-alpha). Accumulation of transforming growth factor-alpha may result from alteration of the endoplasmic reticulum due to storage of hepatitis B virus surface antigen particles. No evidence was found for transforming growth factor-alpha in ground glass hepatocytes to intracellularly interact with the Epidermal Growth Factor Receptor.
Url:
DOI: 10.1016/S0168-8278(96)80139-5
Links to Exploration step
ISTEX:777AF08BEA73B4FB9CE51B8A3903666CA72E25C5Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title>Intracellular accumulation of incompletely processed transforming growth factor-alpha polypeptides in ground glass hepatocytes of chronic hepatitis B virus infection</title><author><name sortKey="Schirmacher, Peter" sort="Schirmacher, Peter" uniqKey="Schirmacher P" first="Peter" last="Schirmacher">Peter Schirmacher</name><affiliation><mods:affiliation>Institute of Pathology, University of Mainz, Mainz, Germany</mods:affiliation></affiliation></author><author><name sortKey="Schau, Dagmar" sort="Schau, Dagmar" uniqKey="Schau D" first="Dagmar" last="Schau">Dagmar Schau</name><affiliation><mods:affiliation>Institute of Pathology, University of Mainz, Mainz, Germany</mods:affiliation></affiliation></author><author><name sortKey="Dienes, Hans Peter" sort="Dienes, Hans Peter" uniqKey="Dienes H" first="Hans Peter" last="Dienes">Hans Peter Dienes</name><affiliation><mods:affiliation>Institute of Pathology, University of Mainz, Mainz, Germany</mods:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:777AF08BEA73B4FB9CE51B8A3903666CA72E25C5</idno><date when="1996" year="1996">1996</date><idno type="doi">10.1016/S0168-8278(96)80139-5</idno><idno type="url">https://api.istex.fr/document/777AF08BEA73B4FB9CE51B8A3903666CA72E25C5/fulltext/pdf</idno><idno type="wicri:Area/Istex/Corpus">000213</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000213</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a">Intracellular accumulation of incompletely processed transforming growth factor-alpha polypeptides in ground glass hepatocytes of chronic hepatitis B virus infection</title><author><name sortKey="Schirmacher, Peter" sort="Schirmacher, Peter" uniqKey="Schirmacher P" first="Peter" last="Schirmacher">Peter Schirmacher</name><affiliation><mods:affiliation>Institute of Pathology, University of Mainz, Mainz, Germany</mods:affiliation></affiliation></author><author><name sortKey="Schau, Dagmar" sort="Schau, Dagmar" uniqKey="Schau D" first="Dagmar" last="Schau">Dagmar Schau</name><affiliation><mods:affiliation>Institute of Pathology, University of Mainz, Mainz, Germany</mods:affiliation></affiliation></author><author><name sortKey="Dienes, Hans Peter" sort="Dienes, Hans Peter" uniqKey="Dienes H" first="Hans Peter" last="Dienes">Hans Peter Dienes</name><affiliation><mods:affiliation>Institute of Pathology, University of Mainz, Mainz, Germany</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">Journal of Hepatology</title><title level="j" type="abbrev">JHEPAT</title><idno type="ISSN">0168-8278</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1995">1995</date><biblScope unit="volume">24</biblScope><biblScope unit="issue">5</biblScope><biblScope unit="page" from="547">547</biblScope><biblScope unit="page" to="554">554</biblScope></imprint><idno type="ISSN">0168-8278</idno></series><idno type="istex">777AF08BEA73B4FB9CE51B8A3903666CA72E25C5</idno><idno type="DOI">10.1016/S0168-8278(96)80139-5</idno><idno type="PII">S0168-8278(96)80139-5</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0168-8278</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Background: Transforming growth factor-alpha is an intracellularly processed and secreted polypeptide that induces a proliferative response in epithelial target cells and represents a potential regulatory factor in embryonic development, liver regeneration, and also hepatocarcinogenesis. We have observed focal transforming growth factor-alpha expression in liver tissues with chronic hepatitis B virus infection. Methods: To further elucidate the nature of this focal transforming growth factor-alpha accumulation were have analyzed overall 23 different liver tissues with chronic hepatitis B virus and hepatitis C virus infection as well as normal liver tissues by immunohistology, ELISA, and Western immunoblot with and without immunoprecipitation. Results: By immunohistology transforming growth factor-alpha polypeptides showed focal subcellular accumulation in ground glass hepatocytes, the histological hallmark of chronic hepatitis B virus infection, in co-localization with HBV-preS1 antigen. By ELISA and Western immunoblot increased tissue concentrations of transforming growth factor-alpha were demonstrated in chronically hepatitis B virus-infected liver tissues with ground glass hepatocytes, especially a 15-kD polypeptide, most likely representing an incompletely processed transforming growth factor-alpha polypeptide. transforming growth factor-alpha retention in ground glass hepatocytes is not a general unspecific effect, since it was not observed for several other secretory liver proteins. Accumulated transforming growth factor-alpha in ground glass hepatocytes does not co-localize with Epidermal Growth Factor Receptor expression. Conclusion: Thus evidence is presented that a principally secreted (viral) polypeptide (HBV-preS1) can interfere with the secretion and processing of a second (cellular) protein (transforming growth factor-alpha). Accumulation of transforming growth factor-alpha may result from alteration of the endoplasmic reticulum due to storage of hepatitis B virus surface antigen particles. No evidence was found for transforming growth factor-alpha in ground glass hepatocytes to intracellularly interact with the Epidermal Growth Factor Receptor.</div></front></TEI><istex><corpusName>elsevier</corpusName><author><json:item><name>Peter Schirmacher</name><affiliations><json:string>Institute of Pathology, University of Mainz, Mainz, Germany</json:string></affiliations></json:item><json:item><name>Dagmar Schauß</name><affiliations><json:string>Institute of Pathology, University of Mainz, Mainz, Germany</json:string></affiliations></json:item><json:item><name>Hans Peter Dienes</name><affiliations><json:string>Institute of Pathology, University of Mainz, Mainz, Germany</json:string></affiliations></json:item></author><subject><json:item><lang><json:string>eng</json:string></lang><value>Growth factor</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Hepatitis</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Hepatitis B Virus</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Hepatocytes</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Transforming growth factor-alpha</value></json:item></subject><language><json:string>eng</json:string></language><abstract>Background: Transforming growth factor-alpha is an intracellularly processed and secreted polypeptide that induces a proliferative response in epithelial target cells and represents a potential regulatory factor in embryonic development, liver regeneration, and also hepatocarcinogenesis. We have observed focal transforming growth factor-alpha expression in liver tissues with chronic hepatitis B virus infection. Methods: To further elucidate the nature of this focal transforming growth factor-alpha accumulation were have analyzed overall 23 different liver tissues with chronic hepatitis B virus and hepatitis C virus infection as well as normal liver tissues by immunohistology, ELISA, and Western immunoblot with and without immunoprecipitation. Results: By immunohistology transforming growth factor-alpha polypeptides showed focal subcellular accumulation in ground glass hepatocytes, the histological hallmark of chronic hepatitis B virus infection, in co-localization with HBV-preS1 antigen. By ELISA and Western immunoblot increased tissue concentrations of transforming growth factor-alpha were demonstrated in chronically hepatitis B virus-infected liver tissues with ground glass hepatocytes, especially a 15-kD polypeptide, most likely representing an incompletely processed transforming growth factor-alpha polypeptide. transforming growth factor-alpha retention in ground glass hepatocytes is not a general unspecific effect, since it was not observed for several other secretory liver proteins. Accumulated transforming growth factor-alpha in ground glass hepatocytes does not co-localize with Epidermal Growth Factor Receptor expression. Conclusion: Thus evidence is presented that a principally secreted (viral) polypeptide (HBV-preS1) can interfere with the secretion and processing of a second (cellular) protein (transforming growth factor-alpha). Accumulation of transforming growth factor-alpha may result from alteration of the endoplasmic reticulum due to storage of hepatitis B virus surface antigen particles. No evidence was found for transforming growth factor-alpha in ground glass hepatocytes to intracellularly interact with the Epidermal Growth Factor Receptor.</abstract><qualityIndicators><score>7.093</score><pdfVersion>1.2</pdfVersion><pdfPageSize>582 x 795 pts</pdfPageSize><refBibsNative>true</refBibsNative><keywordCount>5</keywordCount><abstractCharCount>2197</abstractCharCount><pdfWordCount>4093</pdfWordCount><pdfCharCount>28193</pdfCharCount><pdfPageCount>8</pdfPageCount><abstractWordCount>279</abstractWordCount></qualityIndicators><title>Intracellular accumulation of incompletely processed transforming growth factor-alpha polypeptides in ground glass hepatocytes of chronic hepatitis B virus infection</title><pii><json:string>S0168-8278(96)80139-5</json:string></pii><genre><json:string>research-article</json:string></genre><serie><editor><json:item><name>P Bannasch</name></json:item><json:item><name>D Keppler</name></json:item><json:item><name>G Weber</name></json:item></editor><pages><last>106</last><first>93</first></pages><genre></genre><language><json:string>unknown</json:string></language><title>Liver Call Carcinoma</title></serie><host><volume>24</volume><pii><json:string>S0168-8278(00)X0032-3</json:string></pii><pages><last>554</last><first>547</first></pages><issn><json:string>0168-8278</json:string></issn><issue>5</issue><genre><json:string>Journal</json:string></genre><language><json:string>unknown</json:string></language><title>Journal of Hepatology</title><publicationDate>1996</publicationDate></host><categories><wos><json:string>GASTROENTEROLOGY & HEPATOLOGY</json:string></wos></categories><publicationDate>1995</publicationDate><copyrightDate>1996</copyrightDate><doi><json:string>10.1016/S0168-8278(96)80139-5</json:string></doi><id>777AF08BEA73B4FB9CE51B8A3903666CA72E25C5</id><fulltext><json:item><original>true</original><mimetype>application/pdf</mimetype><extension>pdf</extension><uri>https://api.istex.fr/document/777AF08BEA73B4FB9CE51B8A3903666CA72E25C5/fulltext/pdf</uri></json:item><json:item><original>true</original><mimetype>text/plain</mimetype><extension>txt</extension><uri>https://api.istex.fr/document/777AF08BEA73B4FB9CE51B8A3903666CA72E25C5/fulltext/txt</uri></json:item><json:item><original>false</original><mimetype>application/zip</mimetype><extension>zip</extension><uri>https://api.istex.fr/document/777AF08BEA73B4FB9CE51B8A3903666CA72E25C5/fulltext/zip</uri></json:item><istex:fulltextTEI uri="https://api.istex.fr/document/777AF08BEA73B4FB9CE51B8A3903666CA72E25C5/fulltext/tei"><teiHeader><fileDesc><titleStmt><title level="a">Intracellular accumulation of incompletely processed transforming growth factor-alpha polypeptides in ground glass hepatocytes of chronic hepatitis B virus infection</title></titleStmt><publicationStmt><authority>ISTEX</authority><publisher>ELSEVIER</publisher><availability><p>ELSEVIER</p></availability><date>1996</date></publicationStmt><sourceDesc><biblStruct type="inbook"><analytic><title level="a">Intracellular accumulation of incompletely processed transforming growth factor-alpha polypeptides in ground glass hepatocytes of chronic hepatitis B virus infection</title><author><persName><forename type="first">Peter</forename><surname>Schirmacher</surname></persName><note type="correspondence"><p>Correspondence: Dr. Peter Schirmacher, Institute of Pathology, University Hospital, Langenbeckstr. 1, 55101 Mainz, Germany.</p></note><affiliation>Institute of Pathology, University of Mainz, Mainz, Germany</affiliation></author><author><persName><forename type="first">Dagmar</forename><surname>Schauß</surname></persName><affiliation>Institute of Pathology, University of Mainz, Mainz, Germany</affiliation></author><author><persName><forename type="first">Hans Peter</forename><surname>Dienes</surname></persName><affiliation>Institute of Pathology, University of Mainz, Mainz, Germany</affiliation></author></analytic><monogr><title level="j">Journal of Hepatology</title><title level="j" type="abbrev">JHEPAT</title><idno type="pISSN">0168-8278</idno><idno type="PII">S0168-8278(00)X0032-3</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1995"></date><biblScope unit="volume">24</biblScope><biblScope unit="issue">5</biblScope><biblScope unit="page" from="547">547</biblScope><biblScope unit="page" to="554">554</biblScope></imprint></monogr><idno type="istex">777AF08BEA73B4FB9CE51B8A3903666CA72E25C5</idno><idno type="DOI">10.1016/S0168-8278(96)80139-5</idno><idno type="PII">S0168-8278(96)80139-5</idno></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>1996</date></creation><langUsage><language ident="en">en</language></langUsage><abstract xml:lang="en"><p>Background: Transforming growth factor-alpha is an intracellularly processed and secreted polypeptide that induces a proliferative response in epithelial target cells and represents a potential regulatory factor in embryonic development, liver regeneration, and also hepatocarcinogenesis. We have observed focal transforming growth factor-alpha expression in liver tissues with chronic hepatitis B virus infection. Methods: To further elucidate the nature of this focal transforming growth factor-alpha accumulation were have analyzed overall 23 different liver tissues with chronic hepatitis B virus and hepatitis C virus infection as well as normal liver tissues by immunohistology, ELISA, and Western immunoblot with and without immunoprecipitation. Results: By immunohistology transforming growth factor-alpha polypeptides showed focal subcellular accumulation in ground glass hepatocytes, the histological hallmark of chronic hepatitis B virus infection, in co-localization with HBV-preS1 antigen. By ELISA and Western immunoblot increased tissue concentrations of transforming growth factor-alpha were demonstrated in chronically hepatitis B virus-infected liver tissues with ground glass hepatocytes, especially a 15-kD polypeptide, most likely representing an incompletely processed transforming growth factor-alpha polypeptide. transforming growth factor-alpha retention in ground glass hepatocytes is not a general unspecific effect, since it was not observed for several other secretory liver proteins. Accumulated transforming growth factor-alpha in ground glass hepatocytes does not co-localize with Epidermal Growth Factor Receptor expression. Conclusion: Thus evidence is presented that a principally secreted (viral) polypeptide (HBV-preS1) can interfere with the secretion and processing of a second (cellular) protein (transforming growth factor-alpha). Accumulation of transforming growth factor-alpha may result from alteration of the endoplasmic reticulum due to storage of hepatitis B virus surface antigen particles. No evidence was found for transforming growth factor-alpha in ground glass hepatocytes to intracellularly interact with the Epidermal Growth Factor Receptor.</p></abstract><textClass><keywords scheme="keyword"><list><head>Keywords</head><item><term>Growth factor</term></item><item><term>Hepatitis</term></item><item><term>Hepatitis B Virus</term></item><item><term>Hepatocytes</term></item><item><term>Transforming growth factor-alpha</term></item></list></keywords></textClass></profileDesc><revisionDesc><change when="1995-09-26">Registration</change><change when="1995-09-14">Modified</change><change when="1995">Published</change></revisionDesc></teiHeader></istex:fulltextTEI></fulltext><metadata><istex:metadataXml wicri:clean="Elsevier, elements deleted: tail"><istex:xmlDeclaration>version="1.0" encoding="utf-8"</istex:xmlDeclaration><istex:docType PUBLIC="-//ES//DTD journal article DTD version 4.5.2//EN//XML" URI="art452.dtd" name="istex:docType"></istex:docType><istex:document><converted-article version="4.5.2" docsubtype="fla"><item-info><jid>JHEPAT</jid><aid>96801395</aid><ce:pii>S0168-8278(96)80139-5</ce:pii><ce:doi>10.1016/S0168-8278(96)80139-5</ce:doi><ce:copyright type="unknown" year="1996"></ce:copyright></item-info><head><ce:title>Intracellular accumulation of incompletely processed transforming growth factor-alpha polypeptides in ground glass hepatocytes of chronic hepatitis B virus infection</ce:title><ce:author-group><ce:author><ce:given-name>Peter</ce:given-name><ce:surname>Schirmacher</ce:surname><ce:cross-ref refid="COR1"><ce:sup>∗</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>Dagmar</ce:given-name><ce:surname>Schauß</ce:surname></ce:author><ce:author><ce:given-name>Hans Peter</ce:given-name><ce:surname>Dienes</ce:surname></ce:author><ce:affiliation><ce:textfn>Institute of Pathology, University of Mainz, Mainz, Germany</ce:textfn></ce:affiliation><ce:correspondence id="COR1"><ce:label>*</ce:label><ce:text>Correspondence: Dr. Peter Schirmacher, Institute of Pathology, University Hospital, Langenbeckstr. 1, 55101 Mainz, Germany.</ce:text></ce:correspondence></ce:author-group><ce:date-received day="3" month="3" year="1995"></ce:date-received><ce:date-revised day="14" month="9" year="1995"></ce:date-revised><ce:date-accepted day="26" month="9" year="1995"></ce:date-accepted><ce:abstract><ce:section-title>Abstract</ce:section-title><ce:abstract-sec><ce:simple-para><ce:italic>Background:</ce:italic> Transforming growth factor-alpha is an intracellularly processed and secreted polypeptide that induces a proliferative response in epithelial target cells and represents a potential regulatory factor in embryonic development, liver regeneration, and also hepatocarcinogenesis. We have observed focal transforming growth factor-alpha expression in liver tissues with chronic hepatitis B virus infection.</ce:simple-para><ce:simple-para><ce:italic>Methods:</ce:italic> To further elucidate the nature of this focal transforming growth factor-alpha accumulation were have analyzed overall 23 different liver tissues with chronic hepatitis B virus and hepatitis C virus infection as well as normal liver tissues by immunohistology, ELISA, and Western immunoblot with and without immunoprecipitation.</ce:simple-para><ce:simple-para><ce:italic>Results:</ce:italic> By immunohistology transforming growth factor-alpha polypeptides showed focal subcellular accumulation in ground glass hepatocytes, the histological hallmark of chronic hepatitis B virus infection, in co-localization with HBV-preS1 antigen. By ELISA and Western immunoblot increased tissue concentrations of transforming growth factor-alpha were demonstrated in chronically hepatitis B virus-infected liver tissues with ground glass hepatocytes, especially a 15-kD polypeptide, most likely representing an incompletely processed transforming growth factor-alpha polypeptide. transforming growth factor-alpha retention in ground glass hepatocytes is not a general unspecific effect, since it was not observed for several other secretory liver proteins. Accumulated transforming growth factor-alpha in ground glass hepatocytes does not co-localize with Epidermal Growth Factor Receptor expression.</ce:simple-para><ce:simple-para><ce:italic>Conclusion:</ce:italic> Thus evidence is presented that a principally secreted (viral) polypeptide (HBV-preS1) can interfere with the secretion and processing of a second (cellular) protein (transforming growth factor-alpha). Accumulation of transforming growth factor-alpha may result from alteration of the endoplasmic reticulum due to storage of hepatitis B virus surface antigen particles. No evidence was found for transforming growth factor-alpha in ground glass hepatocytes to intracellularly interact with the Epidermal Growth Factor Receptor.</ce:simple-para></ce:abstract-sec></ce:abstract><ce:keywords><ce:section-title>Keywords</ce:section-title><ce:keyword><ce:text>Growth factor</ce:text></ce:keyword><ce:keyword><ce:text>Hepatitis</ce:text></ce:keyword><ce:keyword><ce:text>Hepatitis B Virus</ce:text></ce:keyword><ce:keyword><ce:text>Hepatocytes</ce:text></ce:keyword><ce:keyword><ce:text>Transforming growth factor-alpha</ce:text></ce:keyword></ce:keywords></head></converted-article></istex:document></istex:metadataXml><mods version="3.6"><titleInfo><title>Intracellular accumulation of incompletely processed transforming growth factor-alpha polypeptides in ground glass hepatocytes of chronic hepatitis B virus infection</title></titleInfo><titleInfo type="alternative" contentType="CDATA"><title>Intracellular accumulation of incompletely processed transforming growth factor-alpha polypeptides in ground glass hepatocytes of chronic hepatitis B virus infection</title></titleInfo><name type="personal"><namePart type="given">Peter</namePart><namePart type="family">Schirmacher</namePart><affiliation>Institute of Pathology, University of Mainz, Mainz, Germany</affiliation><description>Correspondence: Dr. Peter Schirmacher, Institute of Pathology, University Hospital, Langenbeckstr. 1, 55101 Mainz, Germany.</description><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Dagmar</namePart><namePart type="family">Schauß</namePart><affiliation>Institute of Pathology, University of Mainz, Mainz, Germany</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Hans Peter</namePart><namePart type="family">Dienes</namePart><affiliation>Institute of Pathology, University of Mainz, Mainz, Germany</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="Full-length article"></genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">1995</dateIssued><dateValid encoding="w3cdtf">1995-09-26</dateValid><dateModified encoding="w3cdtf">1995-09-14</dateModified><copyrightDate encoding="w3cdtf">1996</copyrightDate></originInfo><language><languageTerm type="code" authority="iso639-2b">eng</languageTerm><languageTerm type="code" authority="rfc3066">en</languageTerm></language><physicalDescription><internetMediaType>text/html</internetMediaType></physicalDescription><abstract lang="en">Background: Transforming growth factor-alpha is an intracellularly processed and secreted polypeptide that induces a proliferative response in epithelial target cells and represents a potential regulatory factor in embryonic development, liver regeneration, and also hepatocarcinogenesis. We have observed focal transforming growth factor-alpha expression in liver tissues with chronic hepatitis B virus infection. Methods: To further elucidate the nature of this focal transforming growth factor-alpha accumulation were have analyzed overall 23 different liver tissues with chronic hepatitis B virus and hepatitis C virus infection as well as normal liver tissues by immunohistology, ELISA, and Western immunoblot with and without immunoprecipitation. Results: By immunohistology transforming growth factor-alpha polypeptides showed focal subcellular accumulation in ground glass hepatocytes, the histological hallmark of chronic hepatitis B virus infection, in co-localization with HBV-preS1 antigen. By ELISA and Western immunoblot increased tissue concentrations of transforming growth factor-alpha were demonstrated in chronically hepatitis B virus-infected liver tissues with ground glass hepatocytes, especially a 15-kD polypeptide, most likely representing an incompletely processed transforming growth factor-alpha polypeptide. transforming growth factor-alpha retention in ground glass hepatocytes is not a general unspecific effect, since it was not observed for several other secretory liver proteins. Accumulated transforming growth factor-alpha in ground glass hepatocytes does not co-localize with Epidermal Growth Factor Receptor expression. Conclusion: Thus evidence is presented that a principally secreted (viral) polypeptide (HBV-preS1) can interfere with the secretion and processing of a second (cellular) protein (transforming growth factor-alpha). Accumulation of transforming growth factor-alpha may result from alteration of the endoplasmic reticulum due to storage of hepatitis B virus surface antigen particles. No evidence was found for transforming growth factor-alpha in ground glass hepatocytes to intracellularly interact with the Epidermal Growth Factor Receptor.</abstract><subject><genre>Keywords</genre><topic>Growth factor</topic><topic>Hepatitis</topic><topic>Hepatitis B Virus</topic><topic>Hepatocytes</topic><topic>Transforming growth factor-alpha</topic></subject><relatedItem type="host"><titleInfo><title>Journal of Hepatology</title></titleInfo><titleInfo type="abbreviated"><title>JHEPAT</title></titleInfo><genre type="Journal">journal</genre><originInfo><dateIssued encoding="w3cdtf">199605</dateIssued></originInfo><identifier type="ISSN">0168-8278</identifier><identifier type="PII">S0168-8278(00)X0032-3</identifier><part><date>199605</date><detail type="volume"><number>24</number><caption>vol.</caption></detail><detail type="issue"><number>5</number><caption>no.</caption></detail><extent unit="issue pages"><start>517</start><end>647</end></extent><extent unit="pages"><start>547</start><end>554</end></extent></part></relatedItem><identifier type="istex">777AF08BEA73B4FB9CE51B8A3903666CA72E25C5</identifier><identifier type="DOI">10.1016/S0168-8278(96)80139-5</identifier><identifier type="PII">S0168-8278(96)80139-5</identifier><recordInfo><recordContentSource>ELSEVIER</recordContentSource></recordInfo></mods></metadata></istex></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Musique/explor/MagnificatV1/Data/Istex/Corpus
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000213 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Istex/Corpus/biblio.hfd -nk 000213 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Musique
|area= MagnificatV1
|flux= Istex
|étape= Corpus
|type= RBID
|clé= ISTEX:777AF08BEA73B4FB9CE51B8A3903666CA72E25C5
|texte= Intracellular accumulation of incompletely processed transforming growth factor-alpha polypeptides in ground glass hepatocytes of chronic hepatitis B virus infection
}}
| This area was generated with Dilib version V0.6.31. |  |