Molecular identification of dolphinfish species (genus Coryphaena ) using multiplex haplotype-specific PCR of mitochondrial DNA
Identifieur interne : 000973 ( Istex/Corpus ); précédent : 000972; suivant : 000974Molecular identification of dolphinfish species (genus Coryphaena ) using multiplex haplotype-specific PCR of mitochondrial DNA
Auteurs : Axayácatl Rocha-Olivares ; Juan Pablo Chávez-GonzálezSource :
- Ichthyological Research [ 1341-8998 ] ; 2008-11-01.
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Abstract
Abstract: A rapid and reliable method of dolphinfish species identification was designed based on PCR amplification of diagnostic DNA fragments from the mitochondrial cytochrome b gene. It consisted in a tetraplex reaction producing a positive control amplicon and species-specific fragments in Coryphaena hippurus and C. equiselis. It was successfully tested in specimens of known identity and in nominal C. hippurus samples among which two C. equiselis were discovered. This approach has significant advantages over other molecular species identification methods and may help in determining species composition of mixed catches, and in forensic and food control applications of dolphinfish specimens or products.
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DOI: 10.1007/s10228-008-0040-7
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ISTEX:E295D81E83A8374E04A6BC1487709D768B9475D8Le document en format XML
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It consisted in a tetraplex reaction producing a positive control amplicon and species-specific fragments in Coryphaena hippurus and C. equiselis. It was successfully tested in specimens of known identity and in nominal C. hippurus samples among which two C. equiselis were discovered. This approach has significant advantages over other molecular species identification methods and may help in determining species composition of mixed catches, and in forensic and food control applications of dolphinfish specimens or products.</p></abstract><textClass xml:lang="en"><keywords scheme="keyword"><list><head>Keywords</head><item><term>Molecular species identification</term></item><item><term>Cytochrome b</term></item><item><term>MHS–PCR</term></item><item><term>Coryphaena hippurus</term></item><item><term>Coryphaena equiselis</term></item></list></keywords></textClass><textClass><keywords scheme="Journal Subject"><list><head>Life Sciences</head><item><term>Freshwater & Marine Ecology</term></item><item><term>Animal Systematics/Taxonomy/Biogeography</term></item><item><term>Ecology</term></item><item><term>Zoology</term></item></list></keywords></textClass></profileDesc><revisionDesc><change when="2007-10-17">Created</change><change when="2008-11-01">Published</change><change 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haplotype-specific PCR of mitochondrial DNA</ArticleTitle><ArticleCategory>Short Report</ArticleCategory><ArticleFirstPage>389</ArticleFirstPage><ArticleLastPage>393</ArticleLastPage><ArticleHistory><RegistrationDate><Year>2008</Year><Month>2</Month><Day>12</Day></RegistrationDate><Received><Year>2007</Year><Month>10</Month><Day>17</Day></Received><Revised><Year>2008</Year><Month>1</Month><Day>15</Day></Revised><Accepted><Year>2008</Year><Month>1</Month><Day>15</Day></Accepted><OnlineDate><Year>2008</Year><Month>3</Month><Day>11</Day></OnlineDate></ArticleHistory><ArticleCopyright><CopyrightHolderName>The Ichthyological Society of Japan</CopyrightHolderName><CopyrightYear>2008</CopyrightYear></ArticleCopyright><ArticleGrants Type="Regular"><MetadataGrant Grant="OpenAccess"></MetadataGrant><AbstractGrant Grant="OpenAccess"></AbstractGrant><BodyPDFGrant Grant="Restricted"></BodyPDFGrant><BodyHTMLGrant Grant="Restricted"></BodyHTMLGrant><BibliographyGrant 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Biology</OrgDivision><OrgName>UMSNH</OrgName><OrgAddress><Street>A.P. 35-A</Street><Postcode>58030</Postcode><City>Morelia</City><State>Michoacán</State><Country Code="MX">Mexico</Country></OrgAddress></Affiliation><Affiliation ID="Aff3"><OrgDivision>Department of Biological Oceanography</OrgDivision><OrgName>CICESE</OrgName><OrgAddress><Postbox>PO Box 434844</Postbox><City>San Diego</City><State>CA</State><Postcode>92143</Postcode><Country Code="US">USA</Country></OrgAddress></Affiliation></AuthorGroup><Abstract ID="Abs1" Language="En" OutputMedium="All"><Heading>Abstract</Heading><Para>A rapid and reliable method of dolphinfish species identification was designed based on PCR amplification of diagnostic DNA fragments from the mitochondrial cytochrome <Emphasis Type="Italic">b</Emphasis> gene. It consisted in a tetraplex reaction producing a positive control amplicon and species-specific fragments in <Emphasis Type="Italic">Coryphaena hippurus</Emphasis> and <Emphasis Type="Italic">C. equiselis</Emphasis>. It was successfully tested in specimens of known identity and in nominal <Emphasis Type="Italic">C. hippurus</Emphasis> samples among which two <Emphasis Type="Italic">C. equiselis</Emphasis> were discovered. This approach has significant advantages over other molecular species identification methods and may help in determining species composition of mixed catches, and in forensic and food control applications of dolphinfish specimens or products.</Para></Abstract><KeywordGroup Language="En"><Heading>Keywords</Heading><Keyword>Molecular species identification</Keyword><Keyword>Cytochrome <Emphasis Type="Italic">b</Emphasis></Keyword><Keyword>MHS–PCR</Keyword><Keyword><Emphasis Type="Italic">Coryphaena hippurus</Emphasis></Keyword><Keyword><Emphasis Type="Italic">Coryphaena equiselis</Emphasis></Keyword></KeywordGroup></ArticleHeader><NoBody></NoBody></Article></Issue></Volume></Journal></Publisher></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Molecular identification of dolphinfish species (genus Coryphaena ) using multiplex haplotype-specific PCR of mitochondrial DNA</title></titleInfo><titleInfo type="alternative" contentType="CDATA" lang="en"><title>Molecular identification of dolphinfish species (genus Coryphaena) using multiplex haplotype-specific PCR of mitochondrial DNA</title></titleInfo><name type="personal" displayLabel="corresp"><namePart type="given">Axayácatl</namePart><namePart type="family">Rocha-Olivares</namePart><affiliation>Department of Biological Oceanography, CICESE, km 107 Carretera Tijuana-Ensenada, 22860, Ensenada, Baja California, Mexico</affiliation><affiliation>Department of Biological Oceanography, CICESE, PO Box 434844, 92143, San Diego, CA, USA</affiliation><affiliation>E-mail: arocha@cicese.mx</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Juan</namePart><namePart type="given">Pablo</namePart><namePart type="family">Chávez-González</namePart><affiliation>Faculty of Biology, UMSNH, A.P. 35-A, 58030, Morelia, Michoacán, Mexico</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="brief-communication" displayLabel="BriefCommunication" authority="ISTEX" authorityURI="https://content-type.data.istex.fr" valueURI="https://content-type.data.istex.fr/ark:/67375/XTP-S9SX2MFS-0">brief-communication</genre><originInfo><publisher>Springer Japan</publisher><place><placeTerm type="text">Japan</placeTerm></place><dateCreated encoding="w3cdtf">2007-10-17</dateCreated><dateIssued encoding="w3cdtf">2008-11-01</dateIssued><dateIssued encoding="w3cdtf">2008</dateIssued><copyrightDate encoding="w3cdtf">2008</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><abstract lang="en">Abstract: A rapid and reliable method of dolphinfish species identification was designed based on PCR amplification of diagnostic DNA fragments from the mitochondrial cytochrome b gene. 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