Bacterial detection in apheresis platelets: blood systems experience with a two‐bottle and one‐bottle culture system
Identifieur interne : 001677 ( Main/Merge ); précédent : 001676; suivant : 001678Bacterial detection in apheresis platelets: blood systems experience with a two‐bottle and one‐bottle culture system
Auteurs : RBID : ISTEX:18B480F614845A92A8D5F1A4F7BA944B9F573889Abstract
BACKGROUND: United Blood Services (UBS) began bacterial testing of platelets (PLTs) using one‐bottle cultures in September 2003. Collection of 7‐day PLTs using two‐bottle cultures began in April 2006. This study compares our experience using both systems. STUDY DESIGN AND METHODS: PLTs from 13 UBS regional centers cultured from September 1, 2003, to September 1, 2007, were included in the analysis. Positive‐signal bottles from a commercially available microbial detection system (BacT/ALERT, bioMérieux) were sent, with corresponding PLTs if available, for confirmatory testing using plate culture media. AABB definitions were used with modifications. RESULTS: A total of 51,265 7‐day PLT collections and 191,521 5‐day PLT collections were tested with bacterial cultures. The overall true‐positive (TP) rate for the two‐bottle system (1:8544) was comparable to the TP rate with the previous one‐bottle system (1:6344). In three of six yield cases, only the anaerobic bottle was positive (two cases of Group D Streptococci, one case of Corynebacterium spp.). The false‐positive (FP) and indeterminate (IND) rates in the anaerobic bottle (1:1767 and 1:1830, respectively) were significantly higher than those in the aerobic bottle (1:6408 and 1:17,088, respectively; p < 0.001). One confirmed transfusion‐related septic reaction, classified as a late TP after investigation, was reported out of 242,786 tested PLT donations. CONCLUSION: The rate of TP cases by the two‐bottle system was not increased over the one‐bottle system, although anaerobic‐bottle‐only positive cases were detected. FP and IND rates were increased in the two‐bottle system, attributable to the anaerobic bottle. Observation of only one documented transfusion‐related septic reaction in 4 years of bacterial screening at UBS is reassuring, although limitations in passive surveillance and higher rates of reactions reported by others indicate the need for continued vigilance.
Url:
DOI: 10.1111/j.1537-2995.2008.01763.x
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: 003D95
- to stream Istex, to step Curation: 003D95
- to stream Istex, to step Checkpoint: 001481
Links to Exploration step
ISTEX:18B480F614845A92A8D5F1A4F7BA944B9F573889Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title>Bacterial detection in apheresis platelets: blood systems experience with a two‐bottle and one‐bottle culture system</title><author><name sortKey="Su, Leon L" uniqKey="Su L">Leon L. Su</name><affiliation><mods:affiliation>From the United Blood Services and Blood Systems, Scottsdale, Arizona; and Blood Systems Research Institute, San Francisco, California.</mods:affiliation><wicri:noCountry code="subField">California.</wicri:noCountry></affiliation><affiliation wicri:level="2"><mods:affiliation>Correspondence: Leon Su, 6210 E. Oak Street, Scottsdale, AZ 85257; e‐mail: lsu@bloodsystems.org.</mods:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Arizona</region></placeName><wicri:cityArea>Correspondence: Leon Su, 6210 E. Oak Street, Scottsdale</wicri:cityArea></affiliation></author><author><name sortKey="Kamel, Hany" uniqKey="Kamel H">Hany Kamel</name><affiliation><mods:affiliation>From the United Blood Services and Blood Systems, Scottsdale, Arizona; and Blood Systems Research Institute, San Francisco, California.</mods:affiliation><wicri:noCountry code="subField">California.</wicri:noCountry></affiliation><affiliation wicri:level="2"><mods:affiliation>Correspondence: Leon Su, 6210 E. Oak Street, Scottsdale, AZ 85257; e‐mail: lsu@bloodsystems.org.</mods:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Arizona</region></placeName><wicri:cityArea>Correspondence: Leon Su, 6210 E. Oak Street, Scottsdale</wicri:cityArea></affiliation></author><author><name sortKey="Custer, Brian" uniqKey="Custer B">Brian Custer</name><affiliation><mods:affiliation>From the United Blood Services and Blood Systems, Scottsdale, Arizona; and Blood Systems Research Institute, San Francisco, California.</mods:affiliation><wicri:noCountry code="subField">California.</wicri:noCountry></affiliation><affiliation wicri:level="2"><mods:affiliation>Correspondence: Leon Su, 6210 E. Oak Street, Scottsdale, AZ 85257; e‐mail: lsu@bloodsystems.org.</mods:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Arizona</region></placeName><wicri:cityArea>Correspondence: Leon Su, 6210 E. Oak Street, Scottsdale</wicri:cityArea></affiliation></author><author><name sortKey="Vanderpool, Sandi" uniqKey="Vanderpool S">Sandi Vanderpool</name><affiliation><mods:affiliation>From the United Blood Services and Blood Systems, Scottsdale, Arizona; and Blood Systems Research Institute, San Francisco, California.</mods:affiliation><wicri:noCountry code="subField">California.</wicri:noCountry></affiliation><affiliation wicri:level="2"><mods:affiliation>Correspondence: Leon Su, 6210 E. Oak Street, Scottsdale, AZ 85257; e‐mail: lsu@bloodsystems.org.</mods:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Arizona</region></placeName><wicri:cityArea>Correspondence: Leon Su, 6210 E. Oak Street, Scottsdale</wicri:cityArea></affiliation></author><author><name sortKey="Harpool, Dennis" uniqKey="Harpool D">Dennis Harpool</name><affiliation><mods:affiliation>From the United Blood Services and Blood Systems, Scottsdale, Arizona; and Blood Systems Research Institute, San Francisco, California.</mods:affiliation><wicri:noCountry code="subField">California.</wicri:noCountry></affiliation><affiliation wicri:level="2"><mods:affiliation>Correspondence: Leon Su, 6210 E. Oak Street, Scottsdale, AZ 85257; e‐mail: lsu@bloodsystems.org.</mods:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Arizona</region></placeName><wicri:cityArea>Correspondence: Leon Su, 6210 E. Oak Street, Scottsdale</wicri:cityArea></affiliation></author><author><name sortKey="Busch, Michael" uniqKey="Busch M">Michael Busch</name><affiliation><mods:affiliation>From the United Blood Services and Blood Systems, Scottsdale, Arizona; and Blood Systems Research Institute, San Francisco, California.</mods:affiliation><wicri:noCountry code="subField">California.</wicri:noCountry></affiliation><affiliation wicri:level="2"><mods:affiliation>Correspondence: Leon Su, 6210 E. Oak Street, Scottsdale, AZ 85257; e‐mail: lsu@bloodsystems.org.</mods:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Arizona</region></placeName><wicri:cityArea>Correspondence: Leon Su, 6210 E. Oak Street, Scottsdale</wicri:cityArea></affiliation></author><author><name sortKey="Tomasulo, Peter" uniqKey="Tomasulo P">Peter Tomasulo</name><affiliation><mods:affiliation>From the United Blood Services and Blood Systems, Scottsdale, Arizona; and Blood Systems Research Institute, San Francisco, California.</mods:affiliation><wicri:noCountry code="subField">California.</wicri:noCountry></affiliation><affiliation wicri:level="2"><mods:affiliation>Correspondence: Leon Su, 6210 E. Oak Street, Scottsdale, AZ 85257; e‐mail: lsu@bloodsystems.org.</mods:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Arizona</region></placeName><wicri:cityArea>Correspondence: Leon Su, 6210 E. Oak Street, Scottsdale</wicri:cityArea></affiliation></author></titleStmt><publicationStmt><idno type="RBID">ISTEX:18B480F614845A92A8D5F1A4F7BA944B9F573889</idno><date when="2008">2008</date><idno type="doi">10.1111/j.1537-2995.2008.01763.x</idno><idno type="url">https://api.istex.fr/document/18B480F614845A92A8D5F1A4F7BA944B9F573889/fulltext/pdf</idno><idno type="wicri:Area/Istex/Corpus">003D95</idno><idno type="wicri:Area/Istex/Curation">003D95</idno><idno type="wicri:Area/Istex/Checkpoint">001481</idno><idno type="MainMerge">001481</idno><idno type="wicri:Area/Main/Merge">001677</idno></publicationStmt><seriesStmt><idno type="ISSN">0041-1132</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="eng">BACKGROUND: United Blood Services (UBS) began bacterial testing of platelets (PLTs) using one‐bottle cultures in September 2003. Collection of 7‐day PLTs using two‐bottle cultures began in April 2006. This study compares our experience using both systems. STUDY DESIGN AND METHODS: PLTs from 13 UBS regional centers cultured from September 1, 2003, to September 1, 2007, were included in the analysis. Positive‐signal bottles from a commercially available microbial detection system (BacT/ALERT, bioMérieux) were sent, with corresponding PLTs if available, for confirmatory testing using plate culture media. AABB definitions were used with modifications. RESULTS: A total of 51,265 7‐day PLT collections and 191,521 5‐day PLT collections were tested with bacterial cultures. The overall true‐positive (TP) rate for the two‐bottle system (1:8544) was comparable to the TP rate with the previous one‐bottle system (1:6344). In three of six yield cases, only the anaerobic bottle was positive (two cases of Group D Streptococci, one case of Corynebacterium spp.). The false‐positive (FP) and indeterminate (IND) rates in the anaerobic bottle (1:1767 and 1:1830, respectively) were significantly higher than those in the aerobic bottle (1:6408 and 1:17,088, respectively; p < 0.001). One confirmed transfusion‐related septic reaction, classified as a late TP after investigation, was reported out of 242,786 tested PLT donations. CONCLUSION: The rate of TP cases by the two‐bottle system was not increased over the one‐bottle system, although anaerobic‐bottle‐only positive cases were detected. FP and IND rates were increased in the two‐bottle system, attributable to the anaerobic bottle. Observation of only one documented transfusion‐related septic reaction in 4 years of bacterial screening at UBS is reassuring, although limitations in passive surveillance and higher rates of reactions reported by others indicate the need for continued vigilance.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Linguistique/explor/CharterV3/Data/Main/Merge
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001677 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Merge/biblio.hfd -nk 001677 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Linguistique
|area= CharterV3
|flux= Main
|étape= Merge
|type= RBID
|clé= ISTEX:18B480F614845A92A8D5F1A4F7BA944B9F573889
|texte= Bacterial detection in apheresis platelets: blood systems experience with a two‐bottle and one‐bottle culture system
}}
| This area was generated with Dilib version V0.6.07. |  |