Developing Transgenic Jatropha Using the SbNHX1 Gene from an Extreme Halophyte for Cultivation in Saline Wasteland
Identifieur interne : 000173 ( Pmc/Curation ); précédent : 000172; suivant : 000174Developing Transgenic Jatropha Using the SbNHX1 Gene from an Extreme Halophyte for Cultivation in Saline Wasteland
Auteurs : Bhavanath Jha ; Avinash Mishra ; Anupama Jha ; Mukul JoshiSource :
- PLoS ONE [ 1932-6203 ] ; 2013.
Abstract
Url:
DOI: 10.1371/journal.pone.0071136
PubMed: 23940703
PubMed Central: 3733712
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Developing Transgenic <italic>Jatropha</italic>
Using the <italic>SbNHX1</italic>
Gene from an Extreme Halophyte for Cultivation in Saline Wasteland</title>
<author><name sortKey="Jha, Bhavanath" sort="Jha, Bhavanath" uniqKey="Jha B" first="Bhavanath" last="Jha">Bhavanath Jha</name>
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<author><name sortKey="Joshi, Mukul" sort="Joshi, Mukul" uniqKey="Joshi M" first="Mukul" last="Joshi">Mukul Joshi</name>
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<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Developing Transgenic <italic>Jatropha</italic>
Using the <italic>SbNHX1</italic>
Gene from an Extreme Halophyte for Cultivation in Saline Wasteland</title>
<author><name sortKey="Jha, Bhavanath" sort="Jha, Bhavanath" uniqKey="Jha B" first="Bhavanath" last="Jha">Bhavanath Jha</name>
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<author><name sortKey="Mishra, Avinash" sort="Mishra, Avinash" uniqKey="Mishra A" first="Avinash" last="Mishra">Avinash Mishra</name>
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<author><name sortKey="Jha, Anupama" sort="Jha, Anupama" uniqKey="Jha A" first="Anupama" last="Jha">Anupama Jha</name>
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<author><name sortKey="Joshi, Mukul" sort="Joshi, Mukul" uniqKey="Joshi M" first="Mukul" last="Joshi">Mukul Joshi</name>
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<series><title level="j">PLoS ONE</title>
<idno type="eISSN">1932-6203</idno>
<imprint><date when="2013">2013</date>
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<front><div type="abstract" xml:lang="en"><p><italic>Jatropha</italic>
is an important second-generation biofuel plant. Salinity is a major factor adversely impacting the growth and yield of several plants including <italic>Jatropha</italic>
. <italic>SbNHX1</italic>
is a vacuolar Na<sup>+</sup>
/H<sup>+</sup>
antiporter gene that compartmentalises excess Na<sup>+</sup>
ions into the vacuole and maintains ion homeostasis. We have previously cloned and characterised the <italic>SbNHX1</italic>
gene from an extreme halophyte, <italic>Salicornia brachiata</italic>
. Transgenic plants of <italic>Jatropha curcas</italic>
with the <italic>SbNHX1</italic>
gene were developed using microprojectile bombardment mediated transformation. Integration of the transgene was confirmed by PCR and Rt-PCR and the copy number was determined by real time qPCR. The present study of engineering salt tolerance in <italic>Jatropha</italic>
is the first report to date. Salt tolerance of the transgenic lines JL2, JL8 and JL19 was confirmed by leaf senescence assay, chlorophyll estimation, plant growth, ion content, electrolyte leakage and malondialdehyde (MDA) content analysis. Transgenic lines showed better salt tolerance than WT up to 200 mM NaCl. Imparting salt tolerance to <italic>Jatropha</italic>
using the <italic>SbNHX1</italic>
gene may open up the possibility of cultivating it in marginal salty land, releasing arable land presently under <italic>Jatropha</italic>
cultivation for agriculture purposes. Apart from this, transgenic <italic>Jatropha</italic>
can be cultivated with brackish water, opening up the possibility of sustainable cultivation of this biofuel plant in salty coastal areas.</p>
</div>
</front>
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<author><name sortKey="Zheng, S" uniqKey="Zheng S">S Zheng</name>
</author>
<author><name sortKey="Wang, X" uniqKey="Wang X">X Wang</name>
</author>
</analytic>
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</author>
</analytic>
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<biblStruct><analytic><author><name sortKey="Lutts, S" uniqKey="Lutts S">S Lutts</name>
</author>
<author><name sortKey="Kinet, Jm" uniqKey="Kinet J">JM Kinet</name>
</author>
<author><name sortKey="Bouharmont, J" uniqKey="Bouharmont J">J Bouharmont</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Hodges, Dm" uniqKey="Hodges D">DM Hodges</name>
</author>
<author><name sortKey="Delong, Jm" uniqKey="Delong J">JM DeLong</name>
</author>
<author><name sortKey="Forney, Cf" uniqKey="Forney C">CF Forney</name>
</author>
<author><name sortKey="Prange, Rk" uniqKey="Prange R">RK Prange</name>
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<pmc article-type="research-article"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-ta">PLoS One</journal-id>
<journal-id journal-id-type="iso-abbrev">PLoS ONE</journal-id>
<journal-id journal-id-type="publisher-id">plos</journal-id>
<journal-id journal-id-type="pmc">plosone</journal-id>
<journal-title-group><journal-title>PLoS ONE</journal-title>
</journal-title-group>
<issn pub-type="epub">1932-6203</issn>
<publisher><publisher-name>Public Library of Science</publisher-name>
<publisher-loc>San Francisco, USA</publisher-loc>
</publisher>
</journal-meta>
<article-meta><article-id pub-id-type="pmid">23940703</article-id>
<article-id pub-id-type="pmc">3733712</article-id>
<article-id pub-id-type="publisher-id">PONE-D-13-22634</article-id>
<article-id pub-id-type="doi">10.1371/journal.pone.0071136</article-id>
<article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject>
</subj-group>
<subj-group subj-group-type="Discipline-v2"><subject>Agriculture</subject>
<subj-group><subject>Biofuels</subject>
<subj-group><subject>Biodiesel</subject>
</subj-group>
</subj-group>
</subj-group>
<subj-group subj-group-type="Discipline-v2"><subject>Biology</subject>
<subj-group><subject>Biotechnology</subject>
<subj-group><subject>Plant Biotechnology</subject>
<subj-group><subject>Transgenic Plants</subject>
</subj-group>
</subj-group>
</subj-group>
</subj-group>
</article-categories>
<title-group><article-title>Developing Transgenic <italic>Jatropha</italic>
Using the <italic>SbNHX1</italic>
Gene from an Extreme Halophyte for Cultivation in Saline Wasteland</article-title>
<alt-title alt-title-type="running-head">Salt Tolerant <italic>Jatropha</italic>
</alt-title>
</title-group>
<contrib-group><contrib contrib-type="author"><name><surname>Jha</surname>
<given-names>Bhavanath</given-names>
</name>
<xref ref-type="aff" rid="aff1"></xref>
<xref ref-type="corresp" rid="cor1"><sup>*</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Mishra</surname>
<given-names>Avinash</given-names>
</name>
<xref ref-type="aff" rid="aff1"></xref>
</contrib>
<contrib contrib-type="author"><name><surname>Jha</surname>
<given-names>Anupama</given-names>
</name>
<xref ref-type="aff" rid="aff1"></xref>
</contrib>
<contrib contrib-type="author"><name><surname>Joshi</surname>
<given-names>Mukul</given-names>
</name>
<xref ref-type="aff" rid="aff1"></xref>
</contrib>
</contrib-group>
<aff id="aff1"><addr-line>Discipline of Marine Biotechnology and Ecology, CSIR-Central Salt and Marine Chemicals Research Institute, Bhavnagar, Gujarat, India</addr-line>
</aff>
<contrib-group><contrib contrib-type="editor"><name><surname>Peccoud</surname>
<given-names>Jean</given-names>
</name>
<role>Editor</role>
<xref ref-type="aff" rid="edit1"></xref>
</contrib>
</contrib-group>
<aff id="edit1"><addr-line>Virginia Tech, United States of America</addr-line>
</aff>
<author-notes><corresp id="cor1">* E-mail: <email>bjha@csmcri.org</email>
</corresp>
<fn fn-type="conflict"><p><bold>Competing Interests: </bold>
The authors have declared that no competing interests exist.</p>
</fn>
<fn fn-type="con"><p>Conceived and designed the experiments: BJ. Performed the experiments: MJ AJ. Analyzed the data: MJ AM. Wrote the paper: MJ AM BJ.</p>
</fn>
</author-notes>
<pub-date pub-type="collection"><year>2013</year>
</pub-date>
<pub-date pub-type="epub"><day>5</day>
<month>8</month>
<year>2013</year>
</pub-date>
<volume>8</volume>
<issue>8</issue>
<elocation-id>e71136</elocation-id>
<history><date date-type="received"><day>29</day>
<month>5</month>
<year>2013</year>
</date>
<date date-type="accepted"><day>2</day>
<month>7</month>
<year>2013</year>
</date>
</history>
<permissions><copyright-year>2013</copyright-year>
<copyright-holder>Jha et al</copyright-holder>
<license><license-p>This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.</license-p>
</license>
</permissions>
<abstract><p><italic>Jatropha</italic>
is an important second-generation biofuel plant. Salinity is a major factor adversely impacting the growth and yield of several plants including <italic>Jatropha</italic>
. <italic>SbNHX1</italic>
is a vacuolar Na<sup>+</sup>
/H<sup>+</sup>
antiporter gene that compartmentalises excess Na<sup>+</sup>
ions into the vacuole and maintains ion homeostasis. We have previously cloned and characterised the <italic>SbNHX1</italic>
gene from an extreme halophyte, <italic>Salicornia brachiata</italic>
. Transgenic plants of <italic>Jatropha curcas</italic>
with the <italic>SbNHX1</italic>
gene were developed using microprojectile bombardment mediated transformation. Integration of the transgene was confirmed by PCR and Rt-PCR and the copy number was determined by real time qPCR. The present study of engineering salt tolerance in <italic>Jatropha</italic>
is the first report to date. Salt tolerance of the transgenic lines JL2, JL8 and JL19 was confirmed by leaf senescence assay, chlorophyll estimation, plant growth, ion content, electrolyte leakage and malondialdehyde (MDA) content analysis. Transgenic lines showed better salt tolerance than WT up to 200 mM NaCl. Imparting salt tolerance to <italic>Jatropha</italic>
using the <italic>SbNHX1</italic>
gene may open up the possibility of cultivating it in marginal salty land, releasing arable land presently under <italic>Jatropha</italic>
cultivation for agriculture purposes. Apart from this, transgenic <italic>Jatropha</italic>
can be cultivated with brackish water, opening up the possibility of sustainable cultivation of this biofuel plant in salty coastal areas.</p>
</abstract>
<funding-group><funding-statement>The financial assistance received from CSIR (<ext-link ext-link-type="uri" xlink:href="http://www.csir.res.in">www.csir.res.in</ext-link>
), New Delhi (CSC0102: TapCoal) is duly acknowledged. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.</funding-statement>
</funding-group>
<counts><page-count count="10"></page-count>
</counts>
</article-meta>
</front>
</pmc>
</record>
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