Links to Exploration step
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">A Landscape and Climate Data Logistic Model of Tsetse Distribution in Kenya</title>
<author><name sortKey="Moore, Nathan" sort="Moore, Nathan" uniqKey="Moore N" first="Nathan" last="Moore">Nathan Moore</name>
<affiliation><nlm:aff id="aff1"><addr-line>Department of Environmental and Resource Sciences, Zhejiang University, Hangzhou, China</addr-line>
</nlm:aff>
</affiliation>
<affiliation><nlm:aff id="aff2"><addr-line>Department of Geography, Center for Global Change and Earth Observations, Michigan State University, East Lansing, Michigan, United States of America</addr-line>
</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Messina, Joseph" sort="Messina, Joseph" uniqKey="Messina J" first="Joseph" last="Messina">Joseph Messina</name>
<affiliation><nlm:aff id="aff2"><addr-line>Department of Geography, Center for Global Change and Earth Observations, Michigan State University, East Lansing, Michigan, United States of America</addr-line>
</nlm:aff>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PMC</idno>
<idno type="pmid">20676406</idno>
<idno type="pmc">2910741</idno>
<idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2910741</idno>
<idno type="RBID">PMC:2910741</idno>
<idno type="doi">10.1371/journal.pone.0011809</idno>
<date when="2010">2010</date>
<idno type="wicri:Area/Pmc/Corpus">000181</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000181</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">A Landscape and Climate Data Logistic Model of Tsetse Distribution in Kenya</title>
<author><name sortKey="Moore, Nathan" sort="Moore, Nathan" uniqKey="Moore N" first="Nathan" last="Moore">Nathan Moore</name>
<affiliation><nlm:aff id="aff1"><addr-line>Department of Environmental and Resource Sciences, Zhejiang University, Hangzhou, China</addr-line>
</nlm:aff>
</affiliation>
<affiliation><nlm:aff id="aff2"><addr-line>Department of Geography, Center for Global Change and Earth Observations, Michigan State University, East Lansing, Michigan, United States of America</addr-line>
</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Messina, Joseph" sort="Messina, Joseph" uniqKey="Messina J" first="Joseph" last="Messina">Joseph Messina</name>
<affiliation><nlm:aff id="aff2"><addr-line>Department of Geography, Center for Global Change and Earth Observations, Michigan State University, East Lansing, Michigan, United States of America</addr-line>
</nlm:aff>
</affiliation>
</author>
</analytic>
<series><title level="j">PLoS ONE</title>
<idno type="eISSN">1932-6203</idno>
<imprint><date when="2010">2010</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass></textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en"><sec><title>Background</title>
<p><italic>Trypanosoma spp</italic>
, biologically transmitted by the tsetse fly in Africa, are a major cause of illness resulting in both high morbidity and mortality among humans, cattle, wild ungulates, and other species. However, tsetse fly distributions change rapidly due to environmental changes, and fine-scale distribution maps are few. Due to data scarcity, most presence/absence estimates in Kenya prior to 2000 are a combination of local reports, entomological knowledge, and topographic information. The availability of tsetse fly abundance data are limited, or at least have not been collected into aggregate, publicly available national datasets. Despite this limitation, other avenues exist for estimating tsetse distributions including remotely sensed data, climate information, and statistical tools.</p>
</sec>
<sec><title>Methodology/Principal Findings</title>
<p>Here we present a logistic regression model of tsetse abundance. The goal of this model is to estimate the distribution of tsetse fly in Kenya in the year 2000, and to provide a method by which to anticipate their future distribution. Multiple predictor variables were tested for significance and for predictive power; ultimately, a parsimonious subset of variables was identified and used to construct the regression model with the 1973 tsetse map. These data were validated against year 2000 Food and Agriculture Organization (FAO) estimates. Mapcurves Goodness-Of-Fit scores were used to evaluate the modeled fly distribution against FAO estimates and against 1973 presence/absence data, each driven by appropriate climate data.</p>
</sec>
<sec><title>Conclusions/Significance</title>
<p>Logistic regression can be effectively used to produce a model that projects fly abundance under elevated greenhouse gas scenarios. This model identifies potential areas for tsetse abandonment and expansion.</p>
</sec>
</div>
</front>
<back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Kristjanson, Pm" uniqKey="Kristjanson P">PM Kristjanson</name>
</author>
<author><name sortKey="Swallow, Bm" uniqKey="Swallow B">BM Swallow</name>
</author>
<author><name sortKey="Rowlands, Gj" uniqKey="Rowlands G">GJ Rowlands</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Ford, J" uniqKey="Ford J">J Ford</name>
</author>
<author><name sortKey="Katondo, Km" uniqKey="Katondo K">KM Katondo</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Hendrickx, G" uniqKey="Hendrickx G">G Hendrickx</name>
</author>
<author><name sortKey="Napala, A" uniqKey="Napala A">A Napala</name>
</author>
<author><name sortKey="Slingenbergh, Jhw" uniqKey="Slingenbergh J">JHW Slingenbergh</name>
</author>
<author><name sortKey="De Deken, R" uniqKey="De Deken R">R De Deken</name>
</author>
<author><name sortKey="J Vercruysse, J" uniqKey="J Vercruysse J">J J. Vercruysse</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Bett, B" uniqKey="Bett B">B Bett</name>
</author>
<author><name sortKey="Irungu, P" uniqKey="Irungu P">P Irungu</name>
</author>
<author><name sortKey="Nyamwaro, So" uniqKey="Nyamwaro S">SO Nyamwaro</name>
</author>
<author><name sortKey="Murilla, G" uniqKey="Murilla G">G Murilla</name>
</author>
<author><name sortKey="Kitala, P" uniqKey="Kitala P">P Kitala</name>
</author>
</analytic>
</biblStruct>
<biblStruct></biblStruct>
<biblStruct><analytic><author><name sortKey="Ford, J" uniqKey="Ford J">J Ford</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Leak, Sga" uniqKey="Leak S">SGA Leak</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Cecchi, G" uniqKey="Cecchi G">G Cecchi</name>
</author>
<author><name sortKey="Mattioli, Rc" uniqKey="Mattioli R">RC Mattioli</name>
</author>
<author><name sortKey="Slingenbergh, J" uniqKey="Slingenbergh J">J Slingenbergh</name>
</author>
<author><name sortKey="De La Rocque, S" uniqKey="De La Rocque S">S De La Rocque</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Hargrove, Jw" uniqKey="Hargrove J">JW Hargrove</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Terblanche, Js" uniqKey="Terblanche J">JS Terblanche</name>
</author>
<author><name sortKey="Clusella Trullas, S" uniqKey="Clusella Trullas S">S Clusella-Trullas</name>
</author>
<author><name sortKey="Deere, Ja" uniqKey="Deere J">JA Deere</name>
</author>
<author><name sortKey="Chown, Sl" uniqKey="Chown S">SL Chown</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Nash, Tam" uniqKey="Nash T">TAM Nash</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Rogers, D" uniqKey="Rogers D">D Rogers</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Pollock, Jn" uniqKey="Pollock J">JN Pollock</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Randolph, S" uniqKey="Randolph S">S Randolph</name>
</author>
<author><name sortKey="Rogers, D" uniqKey="Rogers D">D Rogers</name>
</author>
<author><name sortKey="Kiilu, J" uniqKey="Kiilu J">J Kiilu</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Leak, Sga" uniqKey="Leak S">SGA Leak</name>
</author>
<author><name sortKey="Ejigu, D" uniqKey="Ejigu D">D Ejigu</name>
</author>
<author><name sortKey="Vreysen, Mj" uniqKey="Vreysen M">MJ Vreysen</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Mcdermott, Jj" uniqKey="Mcdermott J">JJ McDermott</name>
</author>
<author><name sortKey="Kristianson, Pm" uniqKey="Kristianson P">PM Kristianson</name>
</author>
<author><name sortKey="Kruska, Rl" uniqKey="Kruska R">RL Kruska</name>
</author>
<author><name sortKey="Reid, Rs" uniqKey="Reid R">RS Reid</name>
</author>
<author><name sortKey="Robinson, Tp" uniqKey="Robinson T">TP Robinson</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Rogers, Dj" uniqKey="Rogers D">DJ Rogers</name>
</author>
<author><name sortKey="Randolph, Se" uniqKey="Randolph S">SE Randolph</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Rogers, Dj" uniqKey="Rogers D">DJ Rogers</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Curran, Pj" uniqKey="Curran P">PJ Curran</name>
</author>
<author><name sortKey="Atkinson, Pm" uniqKey="Atkinson P">PM Atkinson</name>
</author>
<author><name sortKey="Milton, Ej" uniqKey="Milton E">EJ Milton</name>
</author>
<author><name sortKey="Foody, Gm" uniqKey="Foody G">GM Foody</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Rogers, Dj" uniqKey="Rogers D">DJ Rogers</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Williams, B" uniqKey="Williams B">B Williams</name>
</author>
<author><name sortKey="Dransfield, R" uniqKey="Dransfield R">R Dransfield</name>
</author>
<author><name sortKey="Brightwell, R" uniqKey="Brightwell R">R Brightwell</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Wint, W" uniqKey="Wint W">W Wint</name>
</author>
<author><name sortKey="Rogers, D" uniqKey="Rogers D">D Rogers</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Wint, W" uniqKey="Wint W">W Wint</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Reid, Rs" uniqKey="Reid R">RS Reid</name>
</author>
<author><name sortKey="Kruska, Rl" uniqKey="Kruska R">RL Kruska</name>
</author>
<author><name sortKey="Deichmann, U" uniqKey="Deichmann U">U Deichmann</name>
</author>
<author><name sortKey="Thornton, Pk" uniqKey="Thornton P">PK Thornton</name>
</author>
<author><name sortKey="Leak, Sga" uniqKey="Leak S">SGA Leak</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Hargrove, Ww" uniqKey="Hargrove W">WW Hargrove</name>
</author>
<author><name sortKey="Hoffman, Fm" uniqKey="Hoffman F">FM Hoffman</name>
</author>
<author><name sortKey="Hessburg, Pf" uniqKey="Hessburg P">PF Hessburg</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Boko, M" uniqKey="Boko M">M Boko</name>
</author>
<author><name sortKey="Niang, I" uniqKey="Niang I">I Niang</name>
</author>
<author><name sortKey="Nyong, A" uniqKey="Nyong A">A Nyong</name>
</author>
<author><name sortKey="Vogel, C" uniqKey="Vogel C">C Vogel</name>
</author>
<author><name sortKey="Githeko, A" uniqKey="Githeko A">A Githeko</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Burnham, Kp" uniqKey="Burnham K">KP Burnham</name>
</author>
<author><name sortKey="Anderson, Dr" uniqKey="Anderson D">DR Anderson</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Gilbert, M" uniqKey="Gilbert M">M Gilbert</name>
</author>
<author><name sortKey="Co Authors" uniqKey="Co Authors">Co-authors</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Manel, S" uniqKey="Manel S">S Manel</name>
</author>
<author><name sortKey="Dias, Jm" uniqKey="Dias J">JM Dias</name>
</author>
<author><name sortKey="Ormerod, Sj" uniqKey="Ormerod S">SJ Ormerod</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Mitchell, Td" uniqKey="Mitchell T">TD Mitchell</name>
</author>
<author><name sortKey="Jones, Pd" uniqKey="Jones P">PD Jones</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Goovaerts, P" uniqKey="Goovaerts P">P Goovaerts</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Goovaerts, P" uniqKey="Goovaerts P">P Goovaerts</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Cotton, Wr" uniqKey="Cotton W">WR Cotton</name>
</author>
<author><name sortKey="Carrio, Gg" uniqKey="Carrio G">GG Carrio</name>
</author>
<author><name sortKey="Harrington, Jl" uniqKey="Harrington J">JL Harrington</name>
</author>
<author><name sortKey="Jiang, Hl" uniqKey="Jiang H">HL Jiang</name>
</author>
<author><name sortKey="Liston, Ge" uniqKey="Liston G">GE Liston</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Moore, N" uniqKey="Moore N">N Moore</name>
</author>
<author><name sortKey="Torbick, N" uniqKey="Torbick N">N Torbick</name>
</author>
<author><name sortKey="Lofgren, B" uniqKey="Lofgren B">B Lofgren</name>
</author>
<author><name sortKey="Wang, J" uniqKey="Wang J">J Wang</name>
</author>
<author><name sortKey="Pijanowski, B" uniqKey="Pijanowski B">B Pijanowski</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Mcpherson, Jm" uniqKey="Mcpherson J">JM McPherson</name>
</author>
<author><name sortKey="Jetz, W" uniqKey="Jetz W">W Jetz</name>
</author>
<author><name sortKey="Rogers, Dj" uniqKey="Rogers D">DJ Rogers</name>
</author>
</analytic>
</biblStruct>
</listBibl>
</div1>
</back>
</TEI>
<pmc article-type="research-article"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-ta">PLoS One</journal-id>
<journal-id journal-id-type="publisher-id">plos</journal-id>
<journal-id journal-id-type="pmc">plosone</journal-id>
<journal-title-group><journal-title>PLoS ONE</journal-title>
</journal-title-group>
<issn pub-type="epub">1932-6203</issn>
<publisher><publisher-name>Public Library of Science</publisher-name>
<publisher-loc>San Francisco, USA</publisher-loc>
</publisher>
</journal-meta>
<article-meta><article-id pub-id-type="pmid">20676406</article-id>
<article-id pub-id-type="pmc">2910741</article-id>
<article-id pub-id-type="publisher-id">09-PONE-RA-12955R1</article-id>
<article-id pub-id-type="doi">10.1371/journal.pone.0011809</article-id>
<article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject>
</subj-group>
<subj-group subj-group-type="Discipline"><subject>Ecology/Global Change Ecology</subject>
<subject>Mathematics/Statistics</subject>
<subject>Infectious Diseases/Neglected Tropical Diseases</subject>
<subject>Public Health and Epidemiology/Infectious Diseases</subject>
</subj-group>
</article-categories>
<title-group><article-title>A Landscape and Climate Data Logistic Model of Tsetse Distribution in Kenya</article-title>
<alt-title alt-title-type="running-head">Logistic Model of Tsetse</alt-title>
</title-group>
<contrib-group><contrib contrib-type="author"><name><surname>Moore</surname>
<given-names>Nathan</given-names>
</name>
<xref ref-type="aff" rid="aff1"><sup>1</sup>
</xref>
<xref ref-type="aff" rid="aff2"><sup>2</sup>
</xref>
<xref ref-type="corresp" rid="cor1"><sup>*</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Messina</surname>
<given-names>Joseph</given-names>
</name>
<xref ref-type="aff" rid="aff2"><sup>2</sup>
</xref>
</contrib>
</contrib-group>
<aff id="aff1"><label>1</label>
<addr-line>Department of Environmental and Resource Sciences, Zhejiang University, Hangzhou, China</addr-line>
</aff>
<aff id="aff2"><label>2</label>
<addr-line>Department of Geography, Center for Global Change and Earth Observations, Michigan State University, East Lansing, Michigan, United States of America</addr-line>
</aff>
<contrib-group><contrib contrib-type="editor"><name><surname>Jones</surname>
<given-names>James Holland</given-names>
</name>
<role>Editor</role>
<xref ref-type="aff" rid="edit1"></xref>
</contrib>
</contrib-group>
<aff id="edit1">Stanford University, United States of America</aff>
<author-notes><corresp id="cor1">* E-mail: <email>moorena@msu.edu</email>
</corresp>
<fn fn-type="con"><p>Conceived and designed the experiments: NM JM. Performed the experiments: NM. Analyzed the data: NM JM. Wrote the paper: NM JM.</p>
</fn>
</author-notes>
<pub-date pub-type="collection"><year>2010</year>
</pub-date>
<pub-date pub-type="epub"><day>27</day>
<month>7</month>
<year>2010</year>
</pub-date>
<volume>5</volume>
<issue>7</issue>
<elocation-id>e11809</elocation-id>
<history><date date-type="received"><day>17</day>
<month>9</month>
<year>2009</year>
</date>
<date date-type="accepted"><day>29</day>
<month>6</month>
<year>2010</year>
</date>
</history>
<permissions><copyright-statement>Moore, Messina. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.</copyright-statement>
</permissions>
<abstract><sec><title>Background</title>
<p><italic>Trypanosoma spp</italic>
, biologically transmitted by the tsetse fly in Africa, are a major cause of illness resulting in both high morbidity and mortality among humans, cattle, wild ungulates, and other species. However, tsetse fly distributions change rapidly due to environmental changes, and fine-scale distribution maps are few. Due to data scarcity, most presence/absence estimates in Kenya prior to 2000 are a combination of local reports, entomological knowledge, and topographic information. The availability of tsetse fly abundance data are limited, or at least have not been collected into aggregate, publicly available national datasets. Despite this limitation, other avenues exist for estimating tsetse distributions including remotely sensed data, climate information, and statistical tools.</p>
</sec>
<sec><title>Methodology/Principal Findings</title>
<p>Here we present a logistic regression model of tsetse abundance. The goal of this model is to estimate the distribution of tsetse fly in Kenya in the year 2000, and to provide a method by which to anticipate their future distribution. Multiple predictor variables were tested for significance and for predictive power; ultimately, a parsimonious subset of variables was identified and used to construct the regression model with the 1973 tsetse map. These data were validated against year 2000 Food and Agriculture Organization (FAO) estimates. Mapcurves Goodness-Of-Fit scores were used to evaluate the modeled fly distribution against FAO estimates and against 1973 presence/absence data, each driven by appropriate climate data.</p>
</sec>
<sec><title>Conclusions/Significance</title>
<p>Logistic regression can be effectively used to produce a model that projects fly abundance under elevated greenhouse gas scenarios. This model identifies potential areas for tsetse abandonment and expansion.</p>
</sec>
</abstract>
<counts><page-count count="10"></page-count>
</counts>
</article-meta>
</front>
<body><sec id="s1"><title>Introduction</title>
<sec id="s1a"><title>Tsetse background</title>
<p>Trypanosomiasis is a neglected tropical disease that currently represents a major threat to African cattle, costing ∼US$1.3 billion per year <xref ref-type="bibr" rid="pone.0011809-Kristjanson1">[1]</xref>
. The vector for trypanosomiasis, the tsetse fly (<italic>Glossina spp</italic>
), requires a habitat strongly influenced by ecological and climatic features <xref ref-type="bibr" rid="pone.0011809-Ford1">[2]</xref>
, <xref ref-type="bibr" rid="pone.0011809-Hendrickx1">[3]</xref>
— and particularly soil moisture — all of which are influenced by rainfall, soil type, temperature, and other climate variables. Tsetse flies can be categorized into three major subgenera: <italic>palpalis</italic>
(riverine), <italic>fusca</italic>
(forest), and <italic>morsitans</italic>
(savanna). The most common variety of tsetse in East Africa, the <italic>morsitans</italic>
group, primarily feeds on wildlife and cattle, and only occasionally on humans. Infection with trypanosomes may result in clinical disease, known as sleeping sickness in humans and nagana in cattle. However, infection in cattle often goes undiagnosed, and small-area studies show difficulty in relating incidence in cattle with tsetse challenge <xref ref-type="bibr" rid="pone.0011809-Bett1">[4]</xref>
.</p>
<p>Kenya has a long history of tsetse infestation, and with it a heavy economic toll. Livestock production accounts for roughly 8% of Kenya's GDP. In endemic areas, tsetse control could increase livestock productivity by as much as 52% <xref ref-type="bibr" rid="pone.0011809-Kristjanson1">[1]</xref>
, <xref ref-type="bibr" rid="pone.0011809-FAO1">[5]</xref>
. Historical evidence indicates that various attempts to control or eradicate the flies have been hampered by a variety of socioeconomic and geographic factors. Among these geographic factors is elevation (<xref ref-type="fig" rid="pone-0011809-g001">Fig. 1 (A)</xref>
), which is associated with numerous habitats and microclimate zones at different elevations. These microclimates, often associated with scattered tree cover, offer tsetse flies seasonal refugia and access to migrating host species. Specific tree species have long been connected to tsetse-infested areas <xref ref-type="bibr" rid="pone.0011809-Ford2">[6]</xref>
, and recent work has shown that specific vegetation structures and geometry are sought by tsetse flies <xref ref-type="bibr" rid="pone.0011809-Leak1">[7]</xref>
; woody savanna land cover is especially favored <xref ref-type="bibr" rid="pone.0011809-Cecchi1">[8]</xref>
. Such habitats can be identified in part through the remotely sensed Normalized Difference Vegetation Index (NDVI) (<xref ref-type="fig" rid="pone-0011809-g001">Fig. 1 (B)</xref>
) and maximum temperature (<xref ref-type="fig" rid="pone-0011809-g001">Fig. 1(C)</xref>
. Fly larvae can die as a result of drying soils. Temperature extremes, particularly above ∼36°C and below ∼10°C, also lead to adult fly mortality through starvation and water loss via respiration <xref ref-type="bibr" rid="pone.0011809-Hargrove1">[9]</xref>
, <xref ref-type="bibr" rid="pone.0011809-Terblanche1">[10]</xref>
. Low humidity — moisture levels directly related to precipitation (<xref ref-type="fig" rid="pone-0011809-g001">Fig. 1 (D)</xref>
— is also involved in fly mortality, though the exact mechanism is not clear <xref ref-type="bibr" rid="pone.0011809-Hargrove1">[9]</xref>
, <xref ref-type="bibr" rid="pone.0011809-Nash1">[11]</xref>
, <xref ref-type="bibr" rid="pone.0011809-Rogers1">[12]</xref>
. Other important factors affecting tsetse abundance include savanna canopy cover (where flies retreat during daytime heat), presence/absence of host species (notably cattle and/or wildlife <xref ref-type="bibr" rid="pone.0011809-Pollock1">[13]</xref>
–<xref ref-type="bibr" rid="pone.0011809-Leak2">[15]</xref>
), and distance to seasonal refugia. McDermott et al. <xref ref-type="bibr" rid="pone.0011809-McDermott1">[16]</xref>
optimistically noted that increasing human populations could lead to slight declines in tsetse population through habitat loss, particularly in the Lake Victoria basin region. Despite these numerous factors influencing tsetse presence, estimating fly distributions has been difficult.</p>
<fig id="pone-0011809-g001" position="float"><object-id pub-id-type="doi">10.1371/journal.pone.0011809.g001</object-id>
<label>Figure 1</label>
<caption><title>Driving variables.</title>
<p>(A) elevation, (B) renormalized NDVI, (C) 2000 maximum temperature, (D) 2000 annual precipitation.</p>
</caption>
<graphic xlink:href="pone.0011809.g001"></graphic>
</fig>
</sec>
<sec id="s1b"><title>Overview of previous efforts and methods</title>
<p>Several efforts to express the relationships between tsetse abundance, climate and socioeconomic variables have been attempted, with mixed results. A Kenya-wide 1973 survey of tsetse distribution by species based on field reports and other sampling <xref ref-type="bibr" rid="pone.0011809-Ford1">[2]</xref>
is considered among the best maps of fly distribution (hereafter, “1973 data”), but that map is now over 30 years old. More commonly, modeling approaches have been used to estimate fly populations, often using remotely sensed data <xref ref-type="bibr" rid="pone.0011809-Rogers2">[17]</xref>
, <xref ref-type="bibr" rid="pone.0011809-Rogers3">[18]</xref>
, <xref ref-type="bibr" rid="pone.0011809-Curran1">[19]</xref>
. Several simple linear models <xref ref-type="bibr" rid="pone.0011809-Rogers4">[20]</xref>
, <xref ref-type="bibr" rid="pone.0011809-Williams1">[21]</xref>
have been developed, largely with annually averaged data, to identify fly habitats and maximum fly belt extents. More recent research by the Food and Agriculture Organization of the United Nations (FAO) together with the International Atomic Energy Agency (IAEA) (hereafter, “2000 FAO data”) <xref ref-type="bibr" rid="pone.0011809-Hendrickx1">[3]</xref>
, <xref ref-type="bibr" rid="pone.0011809-Wint1">[22]</xref>
, <xref ref-type="bibr" rid="pone.0011809-Wint2">[23]</xref>
employed logistic regressions of remotely sensed data for predicting tsetse abundance to produce a “snapshot” of tsetse distribution at a given point in time. While all of these methods have tried to connect annual data to tsetse distribution, they are hampered by a variety of factors, including irreproducible methods, statistical approaches hampered by excessive multicollinearity, and poor availability or absence of observed data — particularly with respect to tsetse and animal populations. Human population growth also affects land use, primarily through agricultural expansion, which leads to declining tsetse habitat (excluding <italic>palpalis</italic>
species) <xref ref-type="bibr" rid="pone.0011809-Reid1">[24]</xref>
. Significant shifts in tsetse distribution by 2040 are predicted throughout Africa with a ∼7% decrease in overall tsetse population <xref ref-type="bibr" rid="pone.0011809-Reid1">[24]</xref>
. Another hampering factor is seasonal climate; since weather is highly variable in Kenya, tsetse distributions vary similarly. Despite these limitations, almost all of these studies have focused on identifying areas suitable for tsetse survival, and thus implicitly focus on regions where the average annual climate is suitable for tsetse flies to live. However, both climate and climate variability are showing signs of change due to regional climate shifts <xref ref-type="bibr" rid="pone.0011809-McDermott1">[16]</xref>
. If these recent patterns of climate change are responsible for shifts in tsetse distribution, projections of climate change can then be employed to identify areas at particular risk for increased tsetse abundance.</p>
<p>These potential shifts in climate regimes motivate this study. Kenya's government agencies need specific projections of tsetse habitat to establish monitoring and control activities. Given that no published logistic regression model has been made available, and given the need for high-resolution projections, we present here a new model complete with maps of potential tsetse expansion.</p>
</sec>
<sec id="s1c"><title>Purpose of the study</title>
<p>The objective of this study was to estimate the distribution of tsetse flies using a regression model based on remotely sensed data and climate data. No similar model of tsetse abundance as a function of climate data has been presented in the peer-reviewed literature. Our second and parallel objective is to develop a logistic regression model (LM) to incorporate measures of climate variables for predicting future tsetse-susceptible trends in distribution. Ultimately, the final product of this study was to produce a map of regions that may be newly susceptible to tsetse expansion under anticipated variable climate conditions due to greenhouse gases (GHG).</p>
</sec>
</sec>
<sec id="s2"><title>Results</title>
<sec id="s2a"><title>Model construction with 1973 data</title>
<p>Several different independent variables were used to construct the model; a correlation matrix and basic statistics concerning these parameters' roles in the model are given in <xref ref-type="table" rid="pone-0011809-t001">Table 1</xref>
and <xref ref-type="table" rid="pone-0011809-t002">Table 2</xref>
respectively. This set of variables had the best AIC (Akaike Indicator Criterion) score. Most of the variables are significant based on the p-value with the exception of NDVI for May. <bold><italic>Bold italics</italic>
</bold>
in <xref ref-type="table" rid="pone-0011809-t002">Table 2</xref>
are where the standard error is more than 25% the value of the estimated coefficient, which indicates that those variables are often not predictive of tsetse abundance. Land cover (a binary suitable/unsuitable map) was a strong predictor; it also had a relatively large standard error. Other variables and their interaction terms may in some ways replace the role elevation and other omitted terms might have played in predicting tsetse abundance. Wet days and precipitation, which at first glance should be exceedingly collinear, were selected to represent separately the driest months (in the cases of September wet days and February wet days) and rainy periods (in the cases of March+April+May (MAM) precipitation—the “long rains”— and October+November+December (OND) precipitation—the “short rains”. We stress that this is a predictive, not explanatory model, and under such circumstances this is acceptable.</p>
<table-wrap id="pone-0011809-t001" position="float"><object-id pub-id-type="doi">10.1371/journal.pone.0011809.t001</object-id>
<label>Table 1</label>
<caption><title>Correlation Matrix for the broad set of variables considered.</title>
</caption>
<alternatives><graphic id="pone-0011809-t001-1" xlink:href="pone.0011809.t001"></graphic>
<table frame="hsides" rules="groups"><colgroup span="1"><col align="left" span="1"></col>
<col align="center" span="1"></col>
<col align="center" span="1"></col>
<col align="center" span="1"></col>
<col align="center" span="1"></col>
<col align="center" span="1"></col>
<col align="center" span="1"></col>
<col align="center" span="1"></col>
<col align="center" span="1"></col>
<col align="center" span="1"></col>
<col align="center" span="1"></col>
<col align="center" span="1"></col>
<col align="center" span="1"></col>
</colgroup>
<tbody><tr><td align="left" rowspan="1" colspan="1">ELEV</td>
<td align="left" rowspan="1" colspan="1">−0.01</td>
<td align="left" rowspan="1" colspan="1">0.37</td>
<td align="left" rowspan="1" colspan="1">0.50</td>
<td align="left" rowspan="1" colspan="1">0.53</td>
<td align="left" rowspan="1" colspan="1">0.53</td>
<td align="left" rowspan="1" colspan="1">0.19</td>
<td align="left" rowspan="1" colspan="1">0.39</td>
<td align="left" rowspan="1" colspan="1">0.45</td>
<td align="left" rowspan="1" colspan="1">0.50</td>
<td align="left" rowspan="1" colspan="1">0.39</td>
<td align="left" rowspan="1" colspan="1">−0.78</td>
<td align="left" rowspan="1" colspan="1">−0.68</td>
</tr>
<tr><td align="left" rowspan="1" colspan="1">−0.01</td>
<td align="left" rowspan="1" colspan="1">LC</td>
<td align="left" rowspan="1" colspan="1">−0.04</td>
<td align="left" rowspan="1" colspan="1">0.01</td>
<td align="left" rowspan="1" colspan="1">0.03</td>
<td align="left" rowspan="1" colspan="1">0.05</td>
<td align="left" rowspan="1" colspan="1">−0.05</td>
<td align="left" rowspan="1" colspan="1">0.01</td>
<td align="left" rowspan="1" colspan="1">−0.05</td>
<td align="left" rowspan="1" colspan="1">0.00</td>
<td align="left" rowspan="1" colspan="1">−0.03</td>
<td align="left" rowspan="1" colspan="1">0.03</td>
<td align="left" rowspan="1" colspan="1">0.03</td>
</tr>
<tr><td align="left" rowspan="1" colspan="1">0.37</td>
<td align="left" rowspan="1" colspan="1">−0.04</td>
<td align="left" rowspan="1" colspan="1">COW</td>
<td align="left" rowspan="1" colspan="1">0.39</td>
<td align="left" rowspan="1" colspan="1">0.37</td>
<td align="left" rowspan="1" colspan="1">0.36</td>
<td align="left" rowspan="1" colspan="1">0.26</td>
<td align="left" rowspan="1" colspan="1">0.29</td>
<td align="left" rowspan="1" colspan="1">0.17</td>
<td align="left" rowspan="1" colspan="1">0.35</td>
<td align="left" rowspan="1" colspan="1">0.29</td>
<td align="left" rowspan="1" colspan="1">−0.39</td>
<td align="left" rowspan="1" colspan="1">−0.33</td>
</tr>
<tr><td align="left" rowspan="1" colspan="1"><bold>0.50</bold>
</td>
<td align="left" rowspan="1" colspan="1">0.01</td>
<td align="left" rowspan="1" colspan="1">0.39</td>
<td align="left" rowspan="1" colspan="1">ND2</td>
<td align="left" rowspan="1" colspan="1">0.91</td>
<td align="left" rowspan="1" colspan="1">0.87</td>
<td align="left" rowspan="1" colspan="1">0.56</td>
<td align="left" rowspan="1" colspan="1">0.30</td>
<td align="left" rowspan="1" colspan="1">0.24</td>
<td align="left" rowspan="1" colspan="1">0.42</td>
<td align="left" rowspan="1" colspan="1">0.49</td>
<td align="left" rowspan="1" colspan="1">−0.57</td>
<td align="left" rowspan="1" colspan="1">−0.52</td>
</tr>
<tr><td align="left" rowspan="1" colspan="1"><bold>0.53</bold>
</td>
<td align="left" rowspan="1" colspan="1">0.03</td>
<td align="left" rowspan="1" colspan="1">0.37</td>
<td align="left" rowspan="1" colspan="1"><bold>0.91</bold>
</td>
<td align="left" rowspan="1" colspan="1">ND5</td>
<td align="left" rowspan="1" colspan="1">0.93</td>
<td align="left" rowspan="1" colspan="1">0.55</td>
<td align="left" rowspan="1" colspan="1">0.41</td>
<td align="left" rowspan="1" colspan="1">0.11</td>
<td align="left" rowspan="1" colspan="1">0.55</td>
<td align="left" rowspan="1" colspan="1">0.58</td>
<td align="left" rowspan="1" colspan="1">−0.64</td>
<td align="left" rowspan="1" colspan="1">−0.53</td>
</tr>
<tr><td align="left" rowspan="1" colspan="1"><bold>0.53</bold>
</td>
<td align="left" rowspan="1" colspan="1">0.05</td>
<td align="left" rowspan="1" colspan="1">0.36</td>
<td align="left" rowspan="1" colspan="1"><bold>0.87</bold>
</td>
<td align="left" rowspan="1" colspan="1"><bold>0.93</bold>
</td>
<td align="left" rowspan="1" colspan="1">ND10</td>
<td align="left" rowspan="1" colspan="1">0.57</td>
<td align="left" rowspan="1" colspan="1">0.47</td>
<td align="left" rowspan="1" colspan="1">0.05</td>
<td align="left" rowspan="1" colspan="1">0.63</td>
<td align="left" rowspan="1" colspan="1">0.67</td>
<td align="left" rowspan="1" colspan="1">−0.66</td>
<td align="left" rowspan="1" colspan="1">−0.50</td>
</tr>
<tr><td align="left" rowspan="1" colspan="1">0.19</td>
<td align="left" rowspan="1" colspan="1">−0.05</td>
<td align="left" rowspan="1" colspan="1">0.26</td>
<td align="left" rowspan="1" colspan="1"><bold>0.56</bold>
</td>
<td align="left" rowspan="1" colspan="1"><bold>0.55</bold>
</td>
<td align="left" rowspan="1" colspan="1"><bold>0.57</bold>
</td>
<td align="left" rowspan="1" colspan="1">WJAN</td>
<td align="left" rowspan="1" colspan="1">0.45</td>
<td align="left" rowspan="1" colspan="1">0.04</td>
<td align="left" rowspan="1" colspan="1">0.49</td>
<td align="left" rowspan="1" colspan="1">0.68</td>
<td align="left" rowspan="1" colspan="1">−0.48</td>
<td align="left" rowspan="1" colspan="1">−0.46</td>
</tr>
<tr><td align="left" rowspan="1" colspan="1">0.39</td>
<td align="left" rowspan="1" colspan="1">0.01</td>
<td align="left" rowspan="1" colspan="1">0.29</td>
<td align="left" rowspan="1" colspan="1">0.30</td>
<td align="left" rowspan="1" colspan="1">0.41</td>
<td align="left" rowspan="1" colspan="1">0.47</td>
<td align="left" rowspan="1" colspan="1">0.45</td>
<td align="left" rowspan="1" colspan="1">WFEB</td>
<td align="left" rowspan="1" colspan="1">−0.26</td>
<td align="left" rowspan="1" colspan="1">0.66</td>
<td align="left" rowspan="1" colspan="1">0.59</td>
<td align="left" rowspan="1" colspan="1">−0.52</td>
<td align="left" rowspan="1" colspan="1">−0.37</td>
</tr>
<tr><td align="left" rowspan="1" colspan="1">0.45</td>
<td align="left" rowspan="1" colspan="1">−0.05</td>
<td align="left" rowspan="1" colspan="1">0.17</td>
<td align="left" rowspan="1" colspan="1">0.24</td>
<td align="left" rowspan="1" colspan="1">0.11</td>
<td align="left" rowspan="1" colspan="1">0.05</td>
<td align="left" rowspan="1" colspan="1">0.04</td>
<td align="left" rowspan="1" colspan="1">−0.26</td>
<td align="left" rowspan="1" colspan="1">WSEP</td>
<td align="left" rowspan="1" colspan="1">−0.02</td>
<td align="left" rowspan="1" colspan="1">−0.05</td>
<td align="left" rowspan="1" colspan="1">−0.16</td>
<td align="left" rowspan="1" colspan="1">−0.16</td>
</tr>
<tr><td align="left" rowspan="1" colspan="1"><bold>0.50</bold>
</td>
<td align="left" rowspan="1" colspan="1">0.00</td>
<td align="left" rowspan="1" colspan="1">0.35</td>
<td align="left" rowspan="1" colspan="1">0.42</td>
<td align="left" rowspan="1" colspan="1"><bold>0.55</bold>
</td>
<td align="left" rowspan="1" colspan="1"><bold>0.63</bold>
</td>
<td align="left" rowspan="1" colspan="1">0.49</td>
<td align="left" rowspan="1" colspan="1"><bold>0.66</bold>
</td>
<td align="left" rowspan="1" colspan="1">−0.02</td>
<td align="left" rowspan="1" colspan="1">PMAM</td>
<td align="left" rowspan="1" colspan="1">0.79</td>
<td align="left" rowspan="1" colspan="1">−0.68</td>
<td align="left" rowspan="1" colspan="1">−0.42</td>
</tr>
<tr><td align="left" rowspan="1" colspan="1">0.39</td>
<td align="left" rowspan="1" colspan="1">−0.03</td>
<td align="left" rowspan="1" colspan="1">0.29</td>
<td align="left" rowspan="1" colspan="1">0.49</td>
<td align="left" rowspan="1" colspan="1"><bold>0.58</bold>
</td>
<td align="left" rowspan="1" colspan="1"><bold>0.67</bold>
</td>
<td align="left" rowspan="1" colspan="1"><bold>0.68</bold>
</td>
<td align="left" rowspan="1" colspan="1"><bold>0.59</bold>
</td>
<td align="left" rowspan="1" colspan="1">−0.05</td>
<td align="left" rowspan="1" colspan="1"><bold>0.79</bold>
</td>
<td align="left" rowspan="1" colspan="1">POND</td>
<td align="left" rowspan="1" colspan="1">−0.64</td>
<td align="left" rowspan="1" colspan="1">−0.43</td>
</tr>
<tr><td align="left" rowspan="1" colspan="1"><bold>−0.78</bold>
</td>
<td align="left" rowspan="1" colspan="1">0.03</td>
<td align="left" rowspan="1" colspan="1">−0.39</td>
<td align="left" rowspan="1" colspan="1"><bold>−0.57</bold>
</td>
<td align="left" rowspan="1" colspan="1"><bold>−0.64</bold>
</td>
<td align="left" rowspan="1" colspan="1"><bold>−0.66</bold>
</td>
<td align="left" rowspan="1" colspan="1">−0.48</td>
<td align="left" rowspan="1" colspan="1"><bold>−0.52</bold>
</td>
<td align="left" rowspan="1" colspan="1">−0.16</td>
<td align="left" rowspan="1" colspan="1"><bold>−0.68</bold>
</td>
<td align="left" rowspan="1" colspan="1"><bold>−0.64</bold>
</td>
<td align="left" rowspan="1" colspan="1">TJAN</td>
<td align="left" rowspan="1" colspan="1">0.90</td>
</tr>
<tr><td align="left" rowspan="1" colspan="1"><bold>−0.68</bold>
</td>
<td align="left" rowspan="1" colspan="1">0.03</td>
<td align="left" rowspan="1" colspan="1">−0.33</td>
<td align="left" rowspan="1" colspan="1"><bold>−0.52</bold>
</td>
<td align="left" rowspan="1" colspan="1"><bold>−0.53</bold>
</td>
<td align="left" rowspan="1" colspan="1"><bold>−0.50</bold>
</td>
<td align="left" rowspan="1" colspan="1">−0.46</td>
<td align="left" rowspan="1" colspan="1">−0.37</td>
<td align="left" rowspan="1" colspan="1">−0.16</td>
<td align="left" rowspan="1" colspan="1">−0.42</td>
<td align="left" rowspan="1" colspan="1">−0.43</td>
<td align="left" rowspan="1" colspan="1"><bold>0.90</bold>
</td>
<td align="left" rowspan="1" colspan="1">TOCT</td>
</tr>
</tbody>
</table>
</alternatives>
</table-wrap>
<table-wrap id="pone-0011809-t002" position="float"><object-id pub-id-type="doi">10.1371/journal.pone.0011809.t002</object-id>
<label>Table 2</label>
<caption><title>Variables used in the logistic regression and selected statistics.</title>
</caption>
<alternatives><graphic id="pone-0011809-t002-2" xlink:href="pone.0011809.t002"></graphic>
<table frame="hsides" rules="groups"><colgroup span="1"><col align="left" span="1"></col>
<col align="center" span="1"></col>
<col align="center" span="1"></col>
<col align="center" span="1"></col>
<col align="center" span="1"></col>
</colgroup>
<thead><tr><td align="left" rowspan="1" colspan="1"></td>
<td align="left" rowspan="1" colspan="1">Estimate</td>
<td align="left" rowspan="1" colspan="1">Std. Error</td>
<td align="left" rowspan="1" colspan="1">z value</td>
<td align="left" rowspan="1" colspan="1">P</td>
</tr>
</thead>
<tbody><tr><td align="left" rowspan="1" colspan="1">(Intercept)</td>
<td align="left" rowspan="1" colspan="1">−3.70</td>
<td align="left" rowspan="1" colspan="1"><bold><italic>2.83</italic>
</bold>
</td>
<td align="left" rowspan="1" colspan="1">−1.3</td>
<td align="left" rowspan="1" colspan="1">0.2</td>
</tr>
<tr><td align="left" rowspan="1" colspan="1">Land Cover</td>
<td align="left" rowspan="1" colspan="1">2.68</td>
<td align="left" rowspan="1" colspan="1"><bold><italic>0.76</italic>
</bold>
</td>
<td align="left" rowspan="1" colspan="1">3.5</td>
<td align="left" rowspan="1" colspan="1">3.9E-04</td>
</tr>
<tr><td align="left" rowspan="1" colspan="1">ND2</td>
<td align="left" rowspan="1" colspan="1">4.26</td>
<td align="left" rowspan="1" colspan="1"><bold><italic>1.58</italic>
</bold>
</td>
<td align="left" rowspan="1" colspan="1">2.7</td>
<td align="left" rowspan="1" colspan="1">7.1E-03</td>
</tr>
<tr><td align="left" rowspan="1" colspan="1">ND5</td>
<td align="left" rowspan="1" colspan="1">1.19</td>
<td align="left" rowspan="1" colspan="1"><bold><italic>1.50</italic>
</bold>
</td>
<td align="left" rowspan="1" colspan="1">0.8</td>
<td align="left" rowspan="1" colspan="1">0.4</td>
</tr>
<tr><td align="left" rowspan="1" colspan="1">PMAM</td>
<td align="left" rowspan="1" colspan="1">−0.92</td>
<td align="left" rowspan="1" colspan="1">0.16</td>
<td align="left" rowspan="1" colspan="1">−5.6</td>
<td align="left" rowspan="1" colspan="1">2.1E-08</td>
</tr>
<tr><td align="left" rowspan="1" colspan="1">POND</td>
<td align="left" rowspan="1" colspan="1">4.05</td>
<td align="left" rowspan="1" colspan="1">0.29</td>
<td align="left" rowspan="1" colspan="1">13.7</td>
<td align="left" rowspan="1" colspan="1"><2E-16</td>
</tr>
<tr><td align="left" rowspan="1" colspan="1">TJAN</td>
<td align="left" rowspan="1" colspan="1">−0.46</td>
<td align="left" rowspan="1" colspan="1">0.08</td>
<td align="left" rowspan="1" colspan="1">−5.5</td>
<td align="left" rowspan="1" colspan="1">4.7E-08</td>
</tr>
<tr><td align="left" rowspan="1" colspan="1">TOCT</td>
<td align="left" rowspan="1" colspan="1">0.35</td>
<td align="left" rowspan="1" colspan="1">0.03</td>
<td align="left" rowspan="1" colspan="1">10.2</td>
<td align="left" rowspan="1" colspan="1"><2E-16</td>
</tr>
<tr><td align="left" rowspan="1" colspan="1">WJAN</td>
<td align="left" rowspan="1" colspan="1">−0.09</td>
<td align="left" rowspan="1" colspan="1">0.00</td>
<td align="left" rowspan="1" colspan="1">−21.9</td>
<td align="left" rowspan="1" colspan="1"><2E-16</td>
</tr>
<tr><td align="left" rowspan="1" colspan="1">WSEP</td>
<td align="left" rowspan="1" colspan="1">−0.80</td>
<td align="left" rowspan="1" colspan="1">0.11</td>
<td align="left" rowspan="1" colspan="1">−7.6</td>
<td align="left" rowspan="1" colspan="1">3.2E-14</td>
</tr>
<tr><td align="left" rowspan="1" colspan="1">LandCover:ND5</td>
<td align="left" rowspan="1" colspan="1">−1.30</td>
<td align="left" rowspan="1" colspan="1"><bold><italic>0.36</italic>
</bold>
</td>
<td align="left" rowspan="1" colspan="1">−3.7</td>
<td align="left" rowspan="1" colspan="1">2.6E-04</td>
</tr>
<tr><td align="left" rowspan="1" colspan="1">ND2:ND5</td>
<td align="left" rowspan="1" colspan="1">3.73</td>
<td align="left" rowspan="1" colspan="1"><bold><italic>1.00</italic>
</bold>
</td>
<td align="left" rowspan="1" colspan="1">3.7</td>
<td align="left" rowspan="1" colspan="1">2.1E-04</td>
</tr>
<tr><td align="left" rowspan="1" colspan="1">ND2:PMAM</td>
<td align="left" rowspan="1" colspan="1">−0.54</td>
<td align="left" rowspan="1" colspan="1">0.09</td>
<td align="left" rowspan="1" colspan="1">−6.1</td>
<td align="left" rowspan="1" colspan="1">9.1E-10</td>
</tr>
<tr><td align="left" rowspan="1" colspan="1">ND5:PMAM</td>
<td align="left" rowspan="1" colspan="1">0.31</td>
<td align="left" rowspan="1" colspan="1"><bold><italic>0.08</italic>
</bold>
</td>
<td align="left" rowspan="1" colspan="1">3.7</td>
<td align="left" rowspan="1" colspan="1">2.3E-04</td>
</tr>
<tr><td align="left" rowspan="1" colspan="1">PMAM:POND</td>
<td align="left" rowspan="1" colspan="1">−0.02</td>
<td align="left" rowspan="1" colspan="1">0.005</td>
<td align="left" rowspan="1" colspan="1">−4.2</td>
<td align="left" rowspan="1" colspan="1">2.7E-05</td>
</tr>
<tr><td align="left" rowspan="1" colspan="1">LandCover:TJAN</td>
<td align="left" rowspan="1" colspan="1">−0.07</td>
<td align="left" rowspan="1" colspan="1"><bold><italic>0.02</italic>
</bold>
</td>
<td align="left" rowspan="1" colspan="1">−3.2</td>
<td align="left" rowspan="1" colspan="1">1.2E-03</td>
</tr>
<tr><td align="left" rowspan="1" colspan="1">PMAM:TJAN</td>
<td align="left" rowspan="1" colspan="1">0.03</td>
<td align="left" rowspan="1" colspan="1">0.00</td>
<td align="left" rowspan="1" colspan="1">7.5</td>
<td align="left" rowspan="1" colspan="1">6.0E-14</td>
</tr>
<tr><td align="left" rowspan="1" colspan="1">POND:TJAN</td>
<td align="left" rowspan="1" colspan="1">−0.11</td>
<td align="left" rowspan="1" colspan="1">0.01</td>
<td align="left" rowspan="1" colspan="1">−13.2</td>
<td align="left" rowspan="1" colspan="1"><2E-16</td>
</tr>
<tr><td align="left" rowspan="1" colspan="1">TJAN:WJAN</td>
<td align="left" rowspan="1" colspan="1">0.00</td>
<td align="left" rowspan="1" colspan="1">0.00</td>
<td align="left" rowspan="1" colspan="1">22.9</td>
<td align="left" rowspan="1" colspan="1"><2E-16</td>
</tr>
<tr><td align="left" rowspan="1" colspan="1">ND2:WSEP</td>
<td align="left" rowspan="1" colspan="1">0.42</td>
<td align="left" rowspan="1" colspan="1">0.07</td>
<td align="left" rowspan="1" colspan="1">6.2</td>
<td align="left" rowspan="1" colspan="1">4.2E-10</td>
</tr>
<tr><td align="left" rowspan="1" colspan="1">ND5:WSEP</td>
<td align="left" rowspan="1" colspan="1">−0.49</td>
<td align="left" rowspan="1" colspan="1">0.06</td>
<td align="left" rowspan="1" colspan="1">−7.7</td>
<td align="left" rowspan="1" colspan="1">1.3E-14</td>
</tr>
<tr><td align="left" rowspan="1" colspan="1">POND:WSEP</td>
<td align="left" rowspan="1" colspan="1">0.04</td>
<td align="left" rowspan="1" colspan="1">0.00</td>
<td align="left" rowspan="1" colspan="1">10.8</td>
<td align="left" rowspan="1" colspan="1"><2E-16</td>
</tr>
<tr><td align="left" rowspan="1" colspan="1">TJAN:WSEP</td>
<td align="left" rowspan="1" colspan="1">0.02</td>
<td align="left" rowspan="1" colspan="1">0.00</td>
<td align="left" rowspan="1" colspan="1">8.3</td>
<td align="left" rowspan="1" colspan="1"><2E-16</td>
</tr>
<tr><td align="left" rowspan="1" colspan="1">ND2:POND</td>
<td align="left" rowspan="1" colspan="1">−0.54</td>
<td align="left" rowspan="1" colspan="1">0.13</td>
<td align="left" rowspan="1" colspan="1">−4.1</td>
<td align="left" rowspan="1" colspan="1">3.7E-05</td>
</tr>
</tbody>
</table>
</alternatives>
</table-wrap>
<p>Several of these variables change seasonally, particularly NDVI, wet days, precipitation and maximum temperature. However, the primary focus of this study is in understanding tsetse distributions in relation to climate parameters—in this case, precipitation and maximum temperature. Therefore, our results will focus largely on those variables. From <xref ref-type="table" rid="pone-0011809-t003">Table 3</xref>
, MAM precipitation is not significant alone, but instead shows significance in interaction terms with other variables. Temperature for January and October, wet days in September, and their interaction term have very strong predictive power, and indeed are highly collinear. Similarly, NDVI has a significant interaction term with land cover, and it also wields strong predictive power singly and via interactions with climate variables.</p>
<table-wrap id="pone-0011809-t003" position="float"><object-id pub-id-type="doi">10.1371/journal.pone.0011809.t003</object-id>
<label>Table 3</label>
<caption><title>Variables considered in the development of the LM.</title>
</caption>
<alternatives><graphic id="pone-0011809-t003-3" xlink:href="pone.0011809.t003"></graphic>
<table frame="hsides" rules="groups"><colgroup span="1"><col align="left" span="1"></col>
<col align="center" span="1"></col>
<col align="center" span="1"></col>
<col align="center" span="1"></col>
</colgroup>
<thead><tr><td align="left" rowspan="1" colspan="1">Variable</td>
<td align="left" rowspan="1" colspan="1">Time span</td>
<td align="left" rowspan="1" colspan="1">Source</td>
<td align="left" rowspan="1" colspan="1">comments</td>
</tr>
</thead>
<tbody><tr><td align="left" rowspan="1" colspan="1">Monthly maximum Temperature (TMAX)</td>
<td align="left" rowspan="1" colspan="1">Jan–Dec 2000</td>
<td align="left" rowspan="1" colspan="1">CRU TS 2.1</td>
<td align="left" rowspan="1" colspan="1">Kriged to 6 km</td>
</tr>
<tr><td align="left" rowspan="1" colspan="1"><xref ref-type="table-fn" rid="nt101">*</xref>
Monthly minimum Temperature</td>
<td align="left" rowspan="1" colspan="1">Jan–Dec 2000</td>
<td align="left" rowspan="1" colspan="1">CRU TS 2.1</td>
<td align="left" rowspan="1" colspan="1">Kriged to 6 km</td>
</tr>
<tr><td align="left" rowspan="1" colspan="1">Precipitation</td>
<td align="left" rowspan="1" colspan="1">Jan–Dec 2000</td>
<td align="left" rowspan="1" colspan="1">CRU TS 2.1</td>
<td align="left" rowspan="1" colspan="1">Kriged to 6 km</td>
</tr>
<tr><td align="left" rowspan="1" colspan="1">Wet Days</td>
<td align="left" rowspan="1" colspan="1">Jan–Dec 2000</td>
<td align="left" rowspan="1" colspan="1">CRU TS 3.0</td>
<td align="left" rowspan="1" colspan="1">Kriged to 6 km</td>
</tr>
<tr><td align="left" rowspan="1" colspan="1">NDVI</td>
<td align="left" rowspan="1" colspan="1">2000–2007</td>
<td align="left" rowspan="1" colspan="1">MODIS</td>
<td align="left" rowspan="1" colspan="1">16-day imagery</td>
</tr>
<tr><td align="left" rowspan="1" colspan="1"><xref ref-type="table-fn" rid="nt101">*</xref>
Cattle density</td>
<td align="left" rowspan="1" colspan="1">2005 estimate</td>
<td align="left" rowspan="1" colspan="1">FAO glbctd1t0503m</td>
<td align="left" rowspan="1" colspan="1">0.05° resolution</td>
</tr>
<tr><td align="left" rowspan="1" colspan="1"><xref ref-type="table-fn" rid="nt101">*</xref>
Elevation</td>
<td align="left" rowspan="1" colspan="1">1996</td>
<td align="left" rowspan="1" colspan="1">USGS GTOPO30</td>
<td align="left" rowspan="1" colspan="1"></td>
</tr>
<tr><td align="left" rowspan="1" colspan="1">Tsetse distribution</td>
<td align="left" rowspan="1" colspan="1">2000</td>
<td align="left" rowspan="1" colspan="1">PAATIS/FAO</td>
<td align="left" rowspan="1" colspan="1">Continuous;modeled</td>
</tr>
<tr><td align="left" rowspan="1" colspan="1">Tsetse distribution</td>
<td align="left" rowspan="1" colspan="1">1973</td>
<td align="left" rowspan="1" colspan="1">PAATIS/FAO</td>
<td align="left" rowspan="1" colspan="1">Presence/absence</td>
</tr>
<tr><td align="left" rowspan="1" colspan="1">Tsetse distribution</td>
<td align="left" rowspan="1" colspan="1">1996</td>
<td align="left" rowspan="1" colspan="1">PAATIS/FAO</td>
<td align="left" rowspan="1" colspan="1">Presence/absence</td>
</tr>
<tr><td align="left" rowspan="1" colspan="1"><xref ref-type="table-fn" rid="nt101">*</xref>
FAPAR (Fraction of Available Photosynthetically Active Radiation)</td>
<td align="left" rowspan="1" colspan="1">2000–2005</td>
<td align="left" rowspan="1" colspan="1">WDCC Hamburg</td>
<td align="left" rowspan="1" colspan="1">Redundant with NDVI</td>
</tr>
<tr><td align="left" rowspan="1" colspan="1">Land Cover</td>
<td align="left" rowspan="1" colspan="1">2000</td>
<td align="left" rowspan="1" colspan="1">GLC2000</td>
<td align="left" rowspan="1" colspan="1">Binary suitability</td>
</tr>
</tbody>
</table>
</alternatives>
<table-wrap-foot><fn id="nt101"><label></label>
<p>*- examined but ultimately omitted from the model.</p>
</fn>
</table-wrap-foot>
</table-wrap>
<p>Among the more robust surveys is the 1973 assessment, which we used as “ground truth.”</p>
<p>The 1973 observations in <xref ref-type="fig" rid="pone-0011809-g002">Fig. 2 (A)</xref>
are binary presence/absence, and thus the comparison with the LM probability data (B) is stark. Almost uniformly, the LM results predict a greater abundance of flies in areas where no flies (white in A) are observed, with the exception of cooler temperature regions in the highlands; for those values the LM predicts very low or zero probability of tsetse flies. The 1996 estimates, which were based on expansion of the 1973 fly belts, are also shown in <xref ref-type="fig" rid="pone-0011809-g002">Fig. 2 (A)</xref>
for comparison. The high-occurrence conditions in the observed data are along the coastal areas, the slopes of Mount Kenya, the Chyulu Hills, the Mara, and in the Rift Valley. The LM captures that high occurrence well, but overestimates abundance particularly for temperatures between ∼27° and 30°C in the arid east. To compare a binary map with the LM map, we assigned LM values greater than 50% to one, and values less than 50% to zero. The resulting binary difference map in <xref ref-type="fig" rid="pone-0011809-g002">Fig. 2 (C)</xref>
shows overestimation, underestimation, and a “within 50%” estimation. This comparison is admittedly coarse, but it highlights areas where tsetse distributions are poorly modeled. As the Kappa statistic is inappropriate for presence/absence comparisons, we calculated a Mapcurves GOF (“Goodness-Of-Fit”) score <xref ref-type="bibr" rid="pone.0011809-Hargrove2">[25]</xref>
of 0.20 for the area. “Mapcurves GOF” is a statistical tool recently developed for comparing categorical maps <xref ref-type="bibr" rid="pone.0011809-Hargrove2">[25]</xref>
. This statistic, used to compare the similarity of two maps, indicate that the maps “agree” for approximately 20% of the area, “agreement” being in the same 10% bin range. This level is relatively low, but the 1973 observations, which the model is compared against, have several shortcomings as well, discussed below, that makes this GOF level reasonable.</p>
<fig id="pone-0011809-g002" position="float"><object-id pub-id-type="doi">10.1371/journal.pone.0011809.g002</object-id>
<label>Figure 2</label>
<caption><title>Comparison of 1973 fly abundance, modeled and observed.</title>
<p>(A) observed, and (B) modeled tsetse presence for 1973; and (C) difference (B)-(A) showing over-, within-, and under-estimation. 1996 estimates are also outlined in grey (A) for reference.</p>
</caption>
<graphic xlink:href="pone.0011809.g002"></graphic>
</fig>
</sec>
<sec id="s2b"><title>Validating the model with FAO data from 2000</title>
<p>Having built the model and identified areas of disagreement with FAO data, we sought to test the LM against a separate survey of tsetse data for a different time. The LM coefficients were used to construct a year 2000 tsetse distribution based on the selected variables. To evaluate the model's performance, we looked at both the parameter space and the map differences. Of particular interest is the parameter space for precipitation and maximum temperature. <xref ref-type="fig" rid="pone-0011809-g003">Fig. 3</xref>
compares the observed tsetse distribution over average annual precipitation and average annual maximum temperature parameter space showing some similarities and some distinct differences. The overall shape of the distribution is determined by the temperature and precipitation values that occurred in the kriged Climatic Research Unit (CRU) data, so both distributions have the same basic shape. The main differences lie in different color shades that represent fly abundance. For example, near the top center of both graphs are high abundance values (yellow to orange, at a probability of roughly 0.6–0.8). These high-rainfall, fly-prevalent areas correspond to the Indian Ocean coast and the modeled abundance (right panel) shows a strong correspondence with the reported fly abundance (left panel). For some precipitation and temperature combinations, the model over-predicts (e.g. for areas between 33°–35°C) or underpredicts (for areas below ∼26°C). The underpredicting areas are primarily in savanna regions southeast of Nairobi and in southwestern Kenya. The areas overpredicted by the model lie near the Ethiopian border and in isolated pockets north of Nakuru.</p>
<fig id="pone-0011809-g003" position="float"><object-id pub-id-type="doi">10.1371/journal.pone.0011809.g003</object-id>
<label>Figure 3</label>
<caption><title>Parameter space diagrams.</title>
<p>Year 2000 tsetse presence for observed data (left) and modeled data (right). The color scale is probability of fly presence.</p>
</caption>
<graphic xlink:href="pone.0011809.g003"></graphic>
</fig>
<p>FAO and LM estimates, each shown in <xref ref-type="fig" rid="pone-0011809-g004">Fig. 4 (A and B)</xref>
along with the difference between the two (C), contrast markedly in geographically distinct areas. Generally, the FAO estimates in (A) adhere to a more binary distribution—almost presence-absence— whereas the LM estimates have more mid-range values. The differences (<xref ref-type="fig" rid="pone-0011809-g004">Fig. 4(C)</xref>
) point to areas of significant departure between the two estimates, with LM values underestimating PAATIS (the Programme Against African Trypanosomiasis Information System) (brown areas) along major waterways, national park areas, and the well-documented fly belt along the Athi River Valley. The LM model does estimate some fly population in these areas; however, the estimates are muted compared to the FAO data. In some cases (e.g. Kenya's coast near the Somali border) the climate circumstances are suitable for tsetse survival but FAO estimates show low to no abundance. Agreement between the two estimates (white areas) is best in the highland areas, the Rift Valley, Tsavo and the near-coastal areas. A Mapcurves GOF score <xref ref-type="bibr" rid="pone.0011809-Hargrove2">[25]</xref>
, a measure of map similarity, was calculated to be 0.22, which is similar to the Mapcurves GOF score for the 1973 data. We also calculated a 0.14 Kappa statistic.</p>
<fig id="pone-0011809-g004" position="float"><object-id pub-id-type="doi">10.1371/journal.pone.0011809.g004</object-id>
<label>Figure 4</label>
<caption><title>Comparison of 2000 fly abundance, modeled and observed.</title>
<p>(A) Observed and (B) modeled tsetse presence for 2000. (1996 estimates are also outlined in grey for (A) for reference); (C) difference (B-A).</p>
</caption>
<graphic xlink:href="pone.0011809.g004"></graphic>
</fig>
</sec>
<sec id="s2c"><title>Projection under enhanced GHG</title>
<p>We developed a simple estimate of potential tsetse abundance under a single case of elevated GHG based on the model. We applied climate perturbations (future minus current conditions) to the Climatic Research Unit at the University of East Anglia (CRU) 2000 data, which resulted in generally warmer and wetter conditions. The model response was dramatic; as can be seen in <xref ref-type="fig" rid="pone-0011809-g005">Fig. 5 (A)</xref>
, the model indicates suitable habitat for tsetse would be found throughout the heavily populated higher elevations of Kenya.</p>
<fig id="pone-0011809-g005" position="float"><object-id pub-id-type="doi">10.1371/journal.pone.0011809.g005</object-id>
<label>Figure 5</label>
<caption><title>Changes in tsetse abundance due to greenhouse gases.</title>
<p>(A) Projected tsetse presence under 2050 greenhouse gas levels, and (B) percentage change in tsetse presence for elevated greenhouse gases.</p>
</caption>
<graphic xlink:href="pone.0011809.g005"></graphic>
</fig>
<p>The map in <xref ref-type="fig" rid="pone-0011809-g005">Fig. 5 (B)</xref>
shows the difference in projected minus current abundance (<xref ref-type="fig" rid="pone-0011809-g005">Fig. 5 (A)</xref>
– <xref ref-type="fig" rid="pone-0011809-g004">Fig. 4 (B)</xref>
) to show general areas of increased and decreased fly abundance. In addition, increased rainfall in the Lake Turkana region shifts the precipitation/temperature regime towards much higher abundance values in the diagram in <xref ref-type="fig" rid="pone-0011809-g003">Fig. 3</xref>
. The anomalous rise west of Lake Turkana is likely a consequence of drastic rainfall increases in the region, which may be due to errors in climate projection. A similar regime shift occurs along the higher elevation isocline of the eastern highlands, ranging from Mombasa and Kilimanjaro towards Tsavo National Park (yellow areas), and in fly belts north and east of Mt. Kenya up to Marsabit (dark yellow). This shift is towards much higher temperatures at higher elevations (to the upper right of <xref ref-type="fig" rid="pone-0011809-g003">Fig. 3, right panel</xref>
) where fly abundance is currently low. The Kisumu area on to the Rift Valley and north along the Uganda border show decreases in tsetse abundance likely due to warmer temperatures and lower rainfall. Since this is a single projection into the future, however, the overall trends are more salient than the specific locations. In <xref ref-type="fig" rid="pone-0011809-g005">Fig. 5 (B)</xref>
, the model shows tsetse changes following a clear topographic gradient along the eastern scarp stretching from Marsabit to Mt. Kilimanjaro. A decrease in tsetse (increase) in abundance at marginally higher elevations suggests an “uphill migration” along most topographic gradients—including along the Ethiopian border—with only a few areas near the Indian Ocean coast exhibiting a non-topographically-driven shift in tsetse abundance.</p>
</sec>
</sec>
<sec id="s3"><title>Discussion</title>
<sec id="s3a"><title>Aspects of building and validating the model</title>
<p>This logistic regression model was used predict tsetse abundance based on climate and biophysical characteristics. The coefficients (in <xref ref-type="table" rid="pone-0011809-t002">Table 2</xref>
) show some strong positive relationships between tsetse abundance and land cover, February NDVI and “short rains” precipitation. During February, at the end of the dry season, fly abundance should be relatively low in dry areas. Thus, a strong relationship is expected between tsetse abundance and the remaining wet areas represented by these three variables. Negative relationships exist between tsetse abundance and dry season wet days (WJAN and WSEP); this follows from sparse tsetse abundance in areas that receive dry season precipitation, which tend to be dominated by agriculture or high-elevation cool climates. Some coefficients in <xref ref-type="table" rid="pone-0011809-t002">Table 2</xref>
show negative relationships with tsetse abundance that are unexpected, particularly “long rains” precipitation. However, “long rains” precipitation is also highly correlated with agriculture, and agricultural areas are negatively correlated with tsetse abundance as described earlier. Some of the independent variables' coefficients are easy to explain, while others, particularly interaction terms, are less transparent to interpretation.</p>
<p>This type of model is merely predictive, and the coefficients determined by the LM may or may not reflect actual relationships between variables and tsetse abundance. As a result, some regions in the LM results may have errors that can be attributed to a specific cause while other may not. For this reason, we have not attempted to explain or evaluate the roles of specific variables for influencing specific high or low values of tsetse abundance. However, the general trends in tsetse abundance are broadly explained by actual relationships between variables that are known. In some cases— like “long rains” precipitation (PMAM) being a negative predictor, for example— a variable can “stand in” for agriculture or another unknown characteristic. Our selection of model was done by repeatedly examining lots of models to rule out (where possible) clearly unrealistic or illogical coefficients, e.g. a negative coefficient for suitable land cover. Thus, the independent variable coefficients of the final model appear to reflect real relationships with tsetse abundance. This study then tested the resulting tsetse abundance maps against other estimates.</p>
<p>We compared our LM results with the FAO/PAATIS model, which has not been validated. Comparing our LM model to the FAO data assumes that the FAO data correctly capture all instances of fly presence. Thus, our comparisons may be artificially low or high in some regions. Since the main objective of this study is to produce a new tsetse abundance map, differences should be expected. Indeed, by understanding areas of disagreement, we can infer potential areas for on-the-ground fly presence testing that would distinguish between the utility of each model for a given area. In this case, we find some differences and potentially new tsetse distributions.</p>
<p>Mapcurves GOF <xref ref-type="bibr" rid="pone.0011809-Hargrove2">[25]</xref>
scores indicate that the FAO estimates and the LM estimates have some measure of agreement. These similarities arise from both models being driven by similar datasets and from both being constructed in similar ways. However, important differences exist, and these differences may point the way towards building an explanatory model. For example, both models use vegetation (NDVI) as a predictor variable, but the FAO model also uses infrared reflectance and uses a Fourier approach, among other differences. Ecological areas in the FAO model were represented by these remotely sensed data, whereas in the LM we tested actual land cover as a predictor variable. Including land cover in future models is sound because tsetse flies are rarely found in certain types of land cover, e.g. crop-growing areas where preferred food sources are not available. The FAO model's stated goal was “to produce continent wide predictions of the probability of presence of twenty three tsetse species”, whereas our goal differed in region and in species specificity. The maps have relatively low GOF scores, but low GOF scores may merely indicate these different goals.</p>
<p>The disagreement between models means that significant variability exists in even simple models driven by fairly similar datasets. Any modeling at the national scale will need validation data. Our efforts to validate the model against 1973 data yielded a similar GOF score (0.20). The similarity in the GOF scores for FAO, 1973 and 2000 data points to overlaps among all three estimates; we interpret this (along with comparing the map similarities) as meaning that the LM captures major fly abundance areas well, but not necessarily lower abundance areas. There are specific areas in <xref ref-type="fig" rid="pone-0011809-g005">Fig. 5</xref>
that the LM captures poorly—mainly river valleys and cool, wet areas—where lack of a suitable predictor variable like soil moisture in the LM led to the model's failure to develop any predictive power for a specific type of habitat, like river microclimates in otherwise arid regions. Using a different data set than the FAO would probably produce a different set of estimated coefficients, but given the dearth of available data it is unlikely that the Mapcurves GOF scores would improve greatly. Year-to-year variation in tsetse populations, food source distributions, and other sources of variability make a purely logistic model limited to identifying suitable places for tsetse abundance, but not necessarily for tsetse presence. In particular, an averaged 8-year NDVI seasonality (required for use with 1973, prior to remote sensing) clearly decreases signal-to-noise ratio and is a significant source of error. The best application of the LM estimates is to aid in locating potentially suitable areas of tsetse habitation. This information, in conjunction with estimates of seasonal tsetse refugia, can help focus control efforts. These areas are identified as higher-elevation zones adjacent to current fly belts, along with regions that may experience wetter-than-normal conditions in the future. Repeated fly surveillance in these areas would aid both in model improvement and in possible early detection of fly belt expansion.</p>
<p>The lack of numerous tsetse datasets for modeling is a severe constraint. Currently, insufficient fly-trap data exist to validate either model except perhaps in isolated areas. Significant differences are evident between the FAO data and the 1996 “fly belt” maps. Both the FAO probabilities and our LM probabilities are not validated against objective data, and the 1996 estimates, though approximate, are perhaps the closest thing to objective data. Some areas may have high tsetse fly abundance, but data are lacking in the 1996 fly belt map (e.g. Kenya's coast near the Somali border) even though the climate circumstances are suitable and anecdotal reports supportive. Implementing trapping efforts is warranted in locations suggested by these model results and other sources.</p>
</sec>
<sec id="s3b"><title>Potential new tsetse sites</title>
<p>Potential new areas of tsetse abundance suggested by the LM point to elevation/climate gradients. These gradients may be useful for helping establish monitoring transects, particularly in areas that are currently thought to be tsetse-free. Although little early detection of climate effects can be directly attributed to GHG in Kenya <xref ref-type="bibr" rid="pone.0011809-Boko1">[26]</xref>
, real-time detection of tsetse habitat shifts due to weather and climate factors would be identifiable by GIS-based models and carefully controlling for other factors that could enhance changing habitat, like land use and changing food source distributions (i.e. cattle and wildlife). We do not recommend using <xref ref-type="fig" rid="pone-0011809-g005">Fig. 5</xref>
as a projection of future tsetse populations because of three considerations: 1) it is derived from climate impacts of a single GCM, 2) it does not contain any projection of future land use or vegetation distribution, and 3) it lacks explanatory power. However, the model does identify likely gradients for monitoring fly presence/absence; with deterministic vector-based modeling as well as additional climate perturbations from different GCMs, this LM approach may aid in more statistically robust predictions of tsetse abundance. Our next steps will include modeling improvements and interpreting the drivers behind specific changes in tsetse abundance. This leads us to call for more frequent data collection of time-varying climate and vegetation conditions that affect tsetse abundance. In addition, we recommend increasing funding of fly surveillance efforts to assist managing tsetse populations more effectively.</p>
</sec>
</sec>
<sec sec-type="materials|methods" id="s4"><title>Materials and Methods</title>
<sec id="s4a"><title>Logistic Regression Model</title>
<p>Our approach to modeling <italic>Glossina spp.</italic>
distributions began with determining which variables would be best to use in the regression model. To avoid needless collinearity created by simply using all the variables available, we followed recommendations from Burnham and Anderson <xref ref-type="bibr" rid="pone.0011809-Burnham1">[27]</xref>
, which include omitting unrealistic relationships, positing a small set of models first, and avoiding a “just the numbers (let the computer figure it out)” approach. First we examined simple one-variable models to construct a set of meaningful variables for predicting tsetse abundance. Many of these variables were similar to the FAO model (see below). For rainfall, many months had highly similar patterns driven by the “long rains” and the “short rains”. For this reason, precipitation was aggregated together for March through May (PMAM) and for October through December (POND). All monthly temperatures were heavily correlated with one another. Since temperature was also highly correlated with elevation, and because we want to construct a map of tsetse abundance based on climate parameters, we omitted elevation and included temperatures. We retained months where fly mortality is typically highest: January and October, immediately prior to rainy season onset. As a representative of dryness, we included the CRU wet days index for the driest months: January, February, and September. Fraction of Available Photosynthetically Active Radiation (FAPAR) was strongly correlated with NDVI, and was thus omitted at the outset (thus not included in <xref ref-type="table" rid="pone-0011809-t001">Table 1</xref>
or <xref ref-type="table" rid="pone-0011809-t002">Table 2</xref>
) since minimum temperature and maximum temperature were also very tightly correlated. Similarly, to avoid multicollinearity as much as possible, we only used maximum temperature to represent temperature since it is a more common driver of temperature-related tsetse mortality in Kenya than cold temperatures, which occurs only in the highlands.</p>
<p>NDVI data allowed for seasonal changes in land cover to be incorporated into the model. However, since NDVI data for 1973 are unavailable, it would be inappropriate to use 2000 NDVI data alone. To resolve this, we constructed annual average NDVI data from 2000–2007 to represent general seasonal patterns. This averaged NDVI was used for 1973, 2000, and future modeled tsetse distributions. This necessarily reduces model accuracy but allows for inclusion of seasonal greening patterns. Elevation/Topography data (ultimately not used) were from the USGS GTOPO30 digital elevation model (DEM). The FAO 2005 livestock census of cattle was ultimately not used. The 2000 FAO fly data are based on a model, and we are thus using a logistic model to predict another model; however, the 2000 data are the best available, leaving little alternative. Unfortunately, no monthly tsetse fly data were available for any year. Tsetse data for 2000 were gathered and/or estimated by PAATIS <xref ref-type="bibr" rid="pone.0011809-Gilbert1">[28]</xref>
as part of work for the FAO and IAEA. 2000 FAO data overlap with the remote sensing era. To compare the maps we used the Mapcurves GOF score <xref ref-type="bibr" rid="pone.0011809-Hargrove2">[25]</xref>
by sorting data from both maps into 10%-increment bins for the GOF procedure.</p>
<p>Variable names are as follows:</p>
<p>*ELEV = elevation (m)</p>
<p>LC = land cover suitability (binary; 1 = suitable, 0 = not suitable)</p>
<p>*COW = cattle density (head/km<sup>2</sup>
)</p>
<p>ND2 = Normalized Differential Vegetation Index for February (unitless)</p>
<p>ND5 = Normalized Differential Vegetation Index for May (unitless)</p>
<p>*ND10 = Normalized Differential Vegetation Index for October (unitless)</p>
<p>*WJAN = CRU wet days index for January (days)</p>
<p>WFEB = CRU wet days index for February (days)</p>
<p>WSEP = CRU wet days index for September (days)</p>
<p>PMAM = sum of precipitation for March, April, and May</p>
<p>POND = sum of precipitation for October, November, and December</p>
<p>TJAN = average monthly temperature for January</p>
<p>TOCT = average monthly temperature for October</p>
<p>*ultimately removed from the AIC process and the final model</p>
<p>Following the principle of parsimony, we constructed a correlation matrix to remove highly correlated variables. The correlation matrix, shown in <xref ref-type="table" rid="pone-0011809-t001">Table 1</xref>
, was used to diagnose this multicollinearity; here we omitted elevation, October NDVI, and wet days in January. Correlations greater than or equal to 0.5 are in bold. Next we applied a stepwise algorithm called “stepAIC” in R to aid in model selection. This procedure iteratively adds and/or subtracts individual variables or combinations of variables to seek the lowest AIC score. Lower AIC scores are judged the most appropriate models. We only considered the 9 first order terms and several second-order interaction terms, for a total of 35 terms. Stepwise AIC was applied to this initial model.</p>
<p>The following 24 term final model selected through AIC <xref ref-type="bibr" rid="pone.0011809-Burnham1">[27]</xref>
was:<disp-formula><graphic xlink:href="pone.0011809.e001"></graphic>
</disp-formula>
where the x<sub>1</sub>
= LC, x<sub>2</sub>
= ND2, x<sub>3</sub>
= ND5, x<sub>4</sub>
= PMAM, x<sub>5</sub>
= POND, x<sub>6</sub>
= TJAN, x<sub>7</sub>
= TOCT, x<sub>8</sub>
= WJAN and x<sub>9</sub>
= WSEP.</p>
<p>And the coefficients ß<sub>i</sub>
are given in <xref ref-type="table" rid="pone-0011809-t002">Table 2</xref>
. The dependent variable <italic>p</italic>
is tsetse abundance on a scale from zero to one. This exploratory approach mainly focuses on developing a new approach to modeling the tsetse spatial distribution and does not seek to explain causality, nor predict or diagnose tsetse population. The resulting coefficients of the final model determined by the stepwise AIC process are given in <xref ref-type="table" rid="pone-0011809-t002">Table 2</xref>
. Descriptions of the variables considered are outlined in <xref ref-type="table" rid="pone-0011809-t003">Table 3</xref>
.</p>
<p>Following the basic structure of Wint and Rogers <xref ref-type="bibr" rid="pone.0011809-Wint1">[22]</xref>
and Wint <xref ref-type="bibr" rid="pone.0011809-Wint2">[23]</xref>
, this study used logistic regression modeling rather than discriminant analytical/maximum likelihood analysis <xref ref-type="bibr" rid="pone.0011809-Gilbert1">[28]</xref>
, <xref ref-type="bibr" rid="pone.0011809-Manel1">[29]</xref>
. We depart from the Wint and Rogers <xref ref-type="bibr" rid="pone.0011809-Wint1">[22]</xref>
approach by omitting highly correlated variables and removing illogical relationships. We used the R software and several different potentially useful datasets were gathered. All data were initially projected to geographic coordinates. CRU data (TS 3 <xref ref-type="bibr" rid="pone.0011809-Mitchell1">[30]</xref>
) originally at 0.5° resolution, were kriged to 6km following Goovaerts <xref ref-type="bibr" rid="pone.0011809-Goovaerts1">[31]</xref>
, <xref ref-type="bibr" rid="pone.0011809-Goovaerts2">[32]</xref>
, thus 213930 data points were utilized from the original 1283580 data points at 1 km resolution.</p>
<p>Our study considered three time periods and for these, we:</p>
<list list-type="order"><list-item><p>Developed a regression model based on 1973 climate data;</p>
</list-item>
<list-item><p>Assessed the validity of the model based on current (2000) data.</p>
</list-item>
<list-item><p>Estimated tsetse abundance based on projected climate data under elevated GHG.</p>
</list-item>
</list>
<p>To project distributions of tsetse under elevated GHG conditions, we extracted climate data from the National Center for Atmospheric Research's Community Climate System Model (CCSM) version 3.0, namely, precipitation and temperature data for the decades 2000–2009 and 2050–2059. These data were used to drive the Regional Climate Modeling System (RAMS, version 4.4) <xref ref-type="bibr" rid="pone.0011809-Cotton1">[33]</xref>
at 36 km grid spacing and 32 terrain-following vertical levels at a 60-second time step. RAMS phenology was constructed from remotely sensed NDVI, and land cover from the Global Land Cover 2000 dataset. More details on the climate model configuration can be found in Moore et al. <xref ref-type="bibr" rid="pone.0011809-Moore1">[34]</xref>
. These decades were selected to evaluate effects of elevated GHG on climate variables versus those at current GHG levels. To determine the possible extent of tsetse expansion, we used data from the Intergovernmental Panel on Climate Change A1B scenario <xref ref-type="bibr" rid="pone.0011809-Boko1">[26]</xref>
. A1B makes no adjustments from “Business-As-Usual” until CO<sub>2</sub>
concentrations reach 720 ppmv (very aggressive), and as such provides something like an upper bound to potential tsetse distribution. These data from the climate models were <bold>not</bold>
directly used in the LM as inputs; rather, the difference (elevated minus current) was treated as a perturbation added to the CRU data for 2000. Since currently, climate models exhibit no skill at decadal timescales, it is impossible to assert that these data can give rise to projections of tsetse distribution. Instead, we treat these results as a testing of a new modeling approach; and, as identifying areas of sensitivity to tsetse encroachment instead of actual projections of future tsetse distributions. Indeed, without accurate sampling data, model artifacts can arise and severely limit the useful application of model results by agencies and livestock holders <xref ref-type="bibr" rid="pone.0011809-McPherson1">[35]</xref>
. Anticipating future tsetse distributions may allow for targeted control efforts.</p>
</sec>
</sec>
</body>
<back><fn-group><fn fn-type="conflict"><p><bold>Competing Interests: </bold>
The authors have declared that no competing interests exist.</p>
</fn>
<fn fn-type="financial-disclosure"><p><bold>Funding: </bold>
This research was supported by The National Institutes of Health, Office of the Director, Roadmap Initiative, and NIGMS: award # RGM084704A. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of this manuscript.</p>
</fn>
</fn-group>
<ref-list><title>References</title>
<ref id="pone.0011809-Kristjanson1"><label>1</label>
<mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Kristjanson</surname>
<given-names>PM</given-names>
</name>
<name><surname>Swallow</surname>
<given-names>BM</given-names>
</name>
<name><surname>Rowlands</surname>
<given-names>GJ</given-names>
</name>
<etal></etal>
</person-group>
<year>1999</year>
<article-title>‘Measuring the costs of African animal trypanosomiasis, the potential benefits of control and returns to research’,</article-title>
<source>Agric Syst</source>
<volume>59</volume>
<fpage>79</fpage>
<lpage>98</lpage>
</mixed-citation>
</ref>
<ref id="pone.0011809-Ford1"><label>2</label>
<mixed-citation publication-type="book"><person-group person-group-type="author"><name><surname>Ford</surname>
<given-names>J</given-names>
</name>
<name><surname>Katondo</surname>
<given-names>KM</given-names>
</name>
</person-group>
<year>1977</year>
<source>The Distribution of Tsetse Flies (<italic>Glossina</italic>
) in Africa</source>
<publisher-loc>Nairobi</publisher-loc>
<publisher-name>Organisation of African Unity</publisher-name>
</mixed-citation>
</ref>
<ref id="pone.0011809-Hendrickx1"><label>3</label>
<mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Hendrickx</surname>
<given-names>G</given-names>
</name>
<name><surname>Napala</surname>
<given-names>A</given-names>
</name>
<name><surname>Slingenbergh</surname>
<given-names>JHW</given-names>
</name>
<name><surname>De Deken</surname>
<given-names>R</given-names>
</name>
<name><surname>J. Vercruysse</surname>
<given-names>J</given-names>
</name>
<etal></etal>
</person-group>
<year>2000</year>
<article-title>The spatial patterns of trypanosomosis predicted with the aid of satellite imagery.</article-title>
<source>Parasitology</source>
<volume>120</volume>
<fpage>121</fpage>
<lpage>134</lpage>
<pub-id pub-id-type="pmid">10726274</pub-id>
</mixed-citation>
</ref>
<ref id="pone.0011809-Bett1"><label>4</label>
<mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Bett</surname>
<given-names>B</given-names>
</name>
<name><surname>Irungu</surname>
<given-names>P</given-names>
</name>
<name><surname>Nyamwaro</surname>
<given-names>SO</given-names>
</name>
<name><surname>Murilla</surname>
<given-names>G</given-names>
</name>
<name><surname>Kitala</surname>
<given-names>P</given-names>
</name>
<etal></etal>
</person-group>
<year>2008</year>
<article-title>Estimation of tsetse challenge and its relationship with trypanosomosis incidence in cattle kept under pastoral production systems in Kenya.</article-title>
<source>Veterinary Parasitology</source>
<volume>155</volume>
<fpage>287</fpage>
<lpage>298</lpage>
<pub-id pub-id-type="pmid">18602218</pub-id>
</mixed-citation>
</ref>
<ref id="pone.0011809-FAO1"><label>5</label>
<mixed-citation publication-type="journal"><collab>FAO, & AGAL</collab>
<year>2005</year>
<article-title>Livestock Sector Brief.</article-title>
<source>Food and Agriculture Organization of the United Nations & Livestock Information, Sector Analysis and Policy Branch</source>
</mixed-citation>
</ref>
<ref id="pone.0011809-Ford2"><label>6</label>
<mixed-citation publication-type="book"><person-group person-group-type="author"><name><surname>Ford</surname>
<given-names>J</given-names>
</name>
</person-group>
<year>1971</year>
<source>The Role of Trypanosomiases in African Ecology</source>
<publisher-loc>Oxford, UK</publisher-loc>
<publisher-name>Clarendon Press</publisher-name>
<size units="page"></size>
</mixed-citation>
</ref>
<ref id="pone.0011809-Leak1"><label>7</label>
<mixed-citation publication-type="book"><person-group person-group-type="author"><name><surname>Leak</surname>
<given-names>SGA</given-names>
</name>
</person-group>
<year>1999</year>
<source>Tsetse Biology and Ecology</source>
<publisher-loc>New York</publisher-loc>
<publisher-name>CABI Publishing</publisher-name>
<size units="page"></size>
</mixed-citation>
</ref>
<ref id="pone.0011809-Cecchi1"><label>8</label>
<mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Cecchi</surname>
<given-names>G</given-names>
</name>
<name><surname>Mattioli</surname>
<given-names>RC</given-names>
</name>
<name><surname>Slingenbergh</surname>
<given-names>J</given-names>
</name>
<name><surname>De La Rocque</surname>
<given-names>S</given-names>
</name>
</person-group>
<year>2008</year>
<article-title>Land cover and tsetse fly distributions in sub-Saharan Africa.</article-title>
<source>Med Vet Entomol</source>
<volume>22(4)</volume>
<fpage>364</fpage>
<lpage>373</lpage>
<pub-id pub-id-type="pmid">18785934</pub-id>
</mixed-citation>
</ref>
<ref id="pone.0011809-Hargrove1"><label>9</label>
<mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Hargrove</surname>
<given-names>JW</given-names>
</name>
</person-group>
<year>2001</year>
<article-title>Factors affecting density-independent survival of an island population of tsetse flies in Zimbabwe.</article-title>
<source>Entomol Exp Appl</source>
<volume>100(2)</volume>
<fpage>151</fpage>
<lpage>164</lpage>
</mixed-citation>
</ref>
<ref id="pone.0011809-Terblanche1"><label>10</label>
<mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Terblanche</surname>
<given-names>JS</given-names>
</name>
<name><surname>Clusella-Trullas</surname>
<given-names>S</given-names>
</name>
<name><surname>Deere</surname>
<given-names>JA</given-names>
</name>
<name><surname>Chown</surname>
<given-names>SL</given-names>
</name>
</person-group>
<year>2008</year>
<article-title>Thermal tolerance in a south-east African population of the tsetse fly <italic>Glossina pallidipes</italic>
(Diptera, <italic>Glossinidae</italic>
): Implications for forecasting climate change impacts.</article-title>
<source>J Insect Physiol</source>
<volume>54(1)</volume>
<fpage>114</fpage>
<lpage>127</lpage>
<pub-id pub-id-type="pmid">17889900</pub-id>
</mixed-citation>
</ref>
<ref id="pone.0011809-Nash1"><label>11</label>
<mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Nash</surname>
<given-names>TAM</given-names>
</name>
</person-group>
<year>1933</year>
<article-title>A statistical analysis of the climatic factors influencing the density of tsetse flies, <italic>Glossina morsitans Westw</italic>
.</article-title>
<source>J Anim Ecol</source>
<volume>2(2)</volume>
<fpage>197</fpage>
<lpage>203</lpage>
</mixed-citation>
</ref>
<ref id="pone.0011809-Rogers1"><label>12</label>
<mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Rogers</surname>
<given-names>D</given-names>
</name>
</person-group>
<year>1979</year>
<article-title>Tsetse Population Dynamics and Distribution: A New Analytical Approach.</article-title>
<source>J Anim Ecol</source>
<volume>48(3)</volume>
<fpage>825</fpage>
<lpage>849</lpage>
</mixed-citation>
</ref>
<ref id="pone.0011809-Pollock1"><label>13</label>
<mixed-citation publication-type="book"><person-group person-group-type="author"><name><surname>Pollock</surname>
<given-names>JN</given-names>
</name>
</person-group>
<year>1982</year>
<source>Training Manual for Tsetse Control Personnel. Vol. 2: Ecology and Behaviour of Tsetse</source>
<publisher-loc>Rome</publisher-loc>
<publisher-name>Food and agricultural Organization of the United Nations</publisher-name>
<size units="page"></size>
</mixed-citation>
</ref>
<ref id="pone.0011809-Randolph1"><label>14</label>
<mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Randolph</surname>
<given-names>S</given-names>
</name>
<name><surname>Rogers</surname>
<given-names>D</given-names>
</name>
<name><surname>Kiilu</surname>
<given-names>J</given-names>
</name>
</person-group>
<year>1991</year>
<article-title>The feeding behaviour, activity and trappability of wild female <italic>Glossina pallidipes</italic>
in relation to their pregnancy cycle.</article-title>
<source>Med Vet Entomol</source>
<volume>5(3)</volume>
<fpage>335</fpage>
<lpage>350</lpage>
<pub-id pub-id-type="pmid">1768925</pub-id>
</mixed-citation>
</ref>
<ref id="pone.0011809-Leak2"><label>15</label>
<mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Leak</surname>
<given-names>SGA</given-names>
</name>
<name><surname>Ejigu</surname>
<given-names>D</given-names>
</name>
<name><surname>Vreysen</surname>
<given-names>MJ</given-names>
</name>
</person-group>
<year>2008</year>
<article-title>Collection of baseline entomological baseline data for tsetse area-wide integrated pest management programmes.</article-title>
<source>Food and Agriculture Organization of the United Nations</source>
</mixed-citation>
</ref>
<ref id="pone.0011809-McDermott1"><label>16</label>
<mixed-citation publication-type="book"><person-group person-group-type="author"><name><surname>McDermott</surname>
<given-names>JJ</given-names>
</name>
<name><surname>Kristianson</surname>
<given-names>PM</given-names>
</name>
<name><surname>Kruska</surname>
<given-names>RL</given-names>
</name>
<name><surname>Reid</surname>
<given-names>RS</given-names>
</name>
<name><surname>Robinson</surname>
<given-names>TP</given-names>
</name>
<etal></etal>
</person-group>
<year>2001</year>
<article-title>Effects of Climate, Human Population and Socio-Economic Changes on Tsetse-Transmitted Trypanosomiasis to 2050.</article-title>
<person-group person-group-type="editor"><name><surname>Black SJ</surname>
<given-names>SJ</given-names>
</name>
<name><surname>Seed</surname>
<given-names>JR</given-names>
</name>
</person-group>
<source>The African Trypanosomes</source>
<publisher-loc>Boston</publisher-loc>
<publisher-name>Kluwer Academic Publishers</publisher-name>
<size units="page"></size>
</mixed-citation>
</ref>
<ref id="pone.0011809-Rogers2"><label>17</label>
<mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Rogers</surname>
<given-names>DJ</given-names>
</name>
<name><surname>Randolph</surname>
<given-names>SE</given-names>
</name>
</person-group>
<year>1991</year>
<article-title>Mortality rates and population density of tsetse flies correlated with satellite imagery.</article-title>
<source>Nature</source>
<volume>351</volume>
<fpage>739</fpage>
<lpage>41</lpage>
<pub-id pub-id-type="pmid">2062367</pub-id>
</mixed-citation>
</ref>
<ref id="pone.0011809-Rogers3"><label>18</label>
<mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Rogers</surname>
<given-names>DJ</given-names>
</name>
</person-group>
<year>2000</year>
<article-title>Satellites, space, time and the African trypanosomiases.</article-title>
<source>Adv Parasitol</source>
<volume>47</volume>
<fpage>129</fpage>
<lpage>71</lpage>
<pub-id pub-id-type="pmid">10997206</pub-id>
</mixed-citation>
</ref>
<ref id="pone.0011809-Curran1"><label>19</label>
<mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Curran</surname>
<given-names>PJ</given-names>
</name>
<name><surname>Atkinson</surname>
<given-names>PM</given-names>
</name>
<name><surname>Milton</surname>
<given-names>EJ</given-names>
</name>
<name><surname>Foody</surname>
<given-names>GM</given-names>
</name>
</person-group>
<year>2000</year>
<article-title>Linking remote sensing, land cover and disease.</article-title>
<source>Adv Parasitol</source>
<volume>47</volume>
<fpage>37</fpage>
<lpage>80</lpage>
<pub-id pub-id-type="pmid">10997204</pub-id>
</mixed-citation>
</ref>
<ref id="pone.0011809-Rogers4"><label>20</label>
<mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Rogers</surname>
<given-names>DJ</given-names>
</name>
</person-group>
<year>1990</year>
<article-title>A general model for tsetse populations.</article-title>
<source>Insect Science and Its Application</source>
<volume>11 (3)</volume>
<fpage>331</fpage>
<lpage>346</lpage>
</mixed-citation>
</ref>
<ref id="pone.0011809-Williams1"><label>21</label>
<mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Williams</surname>
<given-names>B</given-names>
</name>
<name><surname>Dransfield</surname>
<given-names>R</given-names>
</name>
<name><surname>Brightwell</surname>
<given-names>R</given-names>
</name>
</person-group>
<year>1992</year>
<article-title>The Control of Tsetse Flies in Relation to Fly Movement and Trapping Efficiency.</article-title>
<source>J Appl Ecol</source>
<volume>29 (1)</volume>
<fpage>163</fpage>
<lpage>179</lpage>
</mixed-citation>
</ref>
<ref id="pone.0011809-Wint1"><label>22</label>
<mixed-citation publication-type="other"><person-group person-group-type="author"><name><surname>Wint</surname>
<given-names>W</given-names>
</name>
<name><surname>Rogers</surname>
<given-names>D</given-names>
</name>
</person-group>
<year>2000</year>
<article-title>Predicted Distribution of Tsetse in Africa (pp. 1–62).</article-title>
<comment>Consultants' Report, Rome: Food and agricultural Organization of the United Nations</comment>
</mixed-citation>
</ref>
<ref id="pone.0011809-Wint2"><label>23</label>
<mixed-citation publication-type="other"><person-group person-group-type="author"><name><surname>Wint</surname>
<given-names>W</given-names>
</name>
</person-group>
<year>2001</year>
<source>Kilometre resolution tsetse fly distribution maps for Lake Victoria Basin and West Africa</source>
<comment>Consultants' Report, Vienna, Austria: Food and Agriculture Organisation of the United Nations; IAEA Joint Division, International Atomic Energy Agency</comment>
<size units="page"></size>
</mixed-citation>
</ref>
<ref id="pone.0011809-Reid1"><label>24</label>
<mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Reid</surname>
<given-names>RS</given-names>
</name>
<name><surname>Kruska</surname>
<given-names>RL</given-names>
</name>
<name><surname>Deichmann</surname>
<given-names>U</given-names>
</name>
<name><surname>Thornton</surname>
<given-names>PK</given-names>
</name>
<name><surname>Leak</surname>
<given-names>SGA</given-names>
</name>
</person-group>
<year>2000</year>
<article-title>Human population growth and the extinction of the tsetse fly.</article-title>
<source>Agr Ecosyst Environ</source>
<volume>77 (3)</volume>
<fpage>227</fpage>
<lpage>236</lpage>
<comment>DOI:<ext-link ext-link-type="uri" xlink:href="http://dx.doi.org/10.1016/S0167-8809(99)00103-6">10.1016/S0167-8809(99)00103-6</ext-link>
</comment>
</mixed-citation>
</ref>
<ref id="pone.0011809-Hargrove2"><label>25</label>
<mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Hargrove</surname>
<given-names>WW</given-names>
</name>
<name><surname>Hoffman</surname>
<given-names>FM</given-names>
</name>
<name><surname>Hessburg</surname>
<given-names>PF</given-names>
</name>
</person-group>
<year>2006</year>
<article-title>Mapcurves: a quantitative method for comparing categorical maps.</article-title>
<source>Journal Geogr Syst</source>
<volume>8</volume>
<fpage>187</fpage>
<lpage>208</lpage>
</mixed-citation>
</ref>
<ref id="pone.0011809-Boko1"><label>26</label>
<mixed-citation publication-type="book"><person-group person-group-type="author"><name><surname>Boko</surname>
<given-names>M</given-names>
</name>
<name><surname>Niang</surname>
<given-names>I</given-names>
</name>
<name><surname>Nyong</surname>
<given-names>A</given-names>
</name>
<name><surname>Vogel</surname>
<given-names>C</given-names>
</name>
<name><surname>Githeko</surname>
<given-names>A</given-names>
</name>
<etal></etal>
</person-group>
<year>2007</year>
<article-title>Africa: Climate Change 2007: Impacts, Adaptation and Vulnerability: Contribution of Working Group II.</article-title>
<person-group person-group-type="editor"><name><surname>Parry</surname>
<given-names>ML</given-names>
</name>
<name><surname>Canziani</surname>
<given-names>OF</given-names>
</name>
<name><surname>Palutikof</surname>
<given-names>JP</given-names>
</name>
<name><surname>van der Linden</surname>
<given-names>PJ</given-names>
</name>
<name><surname>Hanson</surname>
<given-names>CE</given-names>
</name>
</person-group>
<source>the 4th Assessment Report of the Intergovernmental Panel on Climate Change</source>
<publisher-loc>Cambridge</publisher-loc>
<publisher-name>Cambridge University Press</publisher-name>
</mixed-citation>
</ref>
<ref id="pone.0011809-Burnham1"><label>27</label>
<mixed-citation publication-type="book"><person-group person-group-type="author"><name><surname>Burnham</surname>
<given-names>KP</given-names>
</name>
<name><surname>Anderson</surname>
<given-names>DR</given-names>
</name>
</person-group>
<year>2002</year>
<source>Model selection and multimodel inference: a practical information-theoretic approach</source>
<publisher-loc>New York</publisher-loc>
<publisher-name>Springer-Verlag</publisher-name>
<size units="page"></size>
</mixed-citation>
</ref>
<ref id="pone.0011809-Gilbert1"><label>28</label>
<mixed-citation publication-type="other"><person-group person-group-type="author"><name><surname>Gilbert</surname>
<given-names>M</given-names>
</name>
<name><surname>Co-authors</surname>
</name>
</person-group>
<year>1999</year>
<article-title>The Development and Use of the Programme Against African Trypanosomiasis Information System (PAAT-IS).</article-title>
<source>Proceedings of the Jubilee Meeting of the International Scientific Council for Trypanosomiasis Research and Control (ISCTRC)</source>
<comment>Mombasa, Kenya, Organization of African Unity–Interafrican Bureau for Animal Resources</comment>
</mixed-citation>
</ref>
<ref id="pone.0011809-Manel1"><label>29</label>
<mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Manel</surname>
<given-names>S</given-names>
</name>
<name><surname>Dias</surname>
<given-names>JM</given-names>
</name>
<name><surname>Ormerod</surname>
<given-names>SJ</given-names>
</name>
</person-group>
<year>1999</year>
<article-title>Comparing discriminant analysis, neural networks and logistic regression for predicting species distributions: a case study with a Himalayan river bird.</article-title>
<source>Ecological Modelling</source>
<volume>120</volume>
<fpage>337</fpage>
<lpage>347</lpage>
</mixed-citation>
</ref>
<ref id="pone.0011809-Mitchell1"><label>30</label>
<mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Mitchell</surname>
<given-names>TD</given-names>
</name>
<name><surname>Jones</surname>
<given-names>PD</given-names>
</name>
</person-group>
<year>2005</year>
<article-title>An improved method of constructing a database of monthly climate observations and associated high-resolution grids.</article-title>
<source>Int J Climatol</source>
<volume>25</volume>
<fpage>693</fpage>
<lpage>712</lpage>
</mixed-citation>
</ref>
<ref id="pone.0011809-Goovaerts1"><label>31</label>
<mixed-citation publication-type="book"><person-group person-group-type="author"><name><surname>Goovaerts</surname>
<given-names>P</given-names>
</name>
</person-group>
<year>1997</year>
<source>Geostatistics for Natural Resources Evaluation</source>
<publisher-loc>New York</publisher-loc>
<publisher-name>Oxford University Press</publisher-name>
<size units="page"></size>
</mixed-citation>
</ref>
<ref id="pone.0011809-Goovaerts2"><label>32</label>
<mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Goovaerts</surname>
<given-names>P</given-names>
</name>
</person-group>
<year>2000</year>
<article-title>Geostatistical approaches for incorporating elevation into the spatial interpolation of rainfall.</article-title>
<source>J Hydrol</source>
<volume>228</volume>
<fpage>113</fpage>
<lpage>129</lpage>
</mixed-citation>
</ref>
<ref id="pone.0011809-Cotton1"><label>33</label>
<mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Cotton</surname>
<given-names>WR</given-names>
</name>
<name><surname>Carrio</surname>
<given-names>GG</given-names>
</name>
<name><surname>Harrington</surname>
<given-names>JL</given-names>
</name>
<name><surname>Jiang</surname>
<given-names>HL</given-names>
</name>
<name><surname>Liston</surname>
<given-names>GE</given-names>
</name>
<etal></etal>
</person-group>
<year>2003</year>
<article-title>RAMS 2001: Current status and future directions.</article-title>
<source>Meteor Atmos Phys</source>
<volume>82</volume>
<fpage>5</fpage>
<lpage>29</lpage>
</mixed-citation>
</ref>
<ref id="pone.0011809-Moore1"><label>34</label>
<mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Moore</surname>
<given-names>N</given-names>
</name>
<name><surname>Torbick</surname>
<given-names>N</given-names>
</name>
<name><surname>Lofgren</surname>
<given-names>B</given-names>
</name>
<name><surname>Wang</surname>
<given-names>J</given-names>
</name>
<name><surname>Pijanowski</surname>
<given-names>B</given-names>
</name>
<etal></etal>
</person-group>
<year>2009</year>
<article-title>Adapting MODIS-derived LAI and fractional cover into the RAMS in East Africa.</article-title>
<source>Int J Climatol</source>
<comment>doi:<ext-link ext-link-type="uri" xlink:href="http://dx.doi.org/10.1002/joc.2011">10.1002/joc.2011</ext-link>
</comment>
</mixed-citation>
</ref>
<ref id="pone.0011809-McPherson1"><label>35</label>
<mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>McPherson</surname>
<given-names>JM</given-names>
</name>
<name><surname>Jetz</surname>
<given-names>W</given-names>
</name>
<name><surname>Rogers</surname>
<given-names>DJ</given-names>
</name>
</person-group>
<year>2004</year>
<article-title>The effects of species' range sizes on the accuracy of distribution models: ecological phenomenon or statistical artefact?</article-title>
<source>J Appl Ecol</source>
<volume>41</volume>
<fpage>811</fpage>
<lpage>823</lpage>
</mixed-citation>
</ref>
</ref-list>
</back>
</pmc>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Agronomie/explor/SisAgriV1/Data/Pmc/Corpus
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000181 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Pmc/Corpus/biblio.hfd -nk 000181 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Wicri/Agronomie |area= SisAgriV1 |flux= Pmc |étape= Corpus |type= RBID |clé= |texte= }}
![]() | This area was generated with Dilib version V0.6.28. | ![]() |