Collection and genetic characterization of Vitis vinifera ’ Žilavka’ by microsatellites and AFLP markers
Identifieur interne : 001159 ( Istex/Corpus ); précédent : 001158; suivant : 001160Collection and genetic characterization of Vitis vinifera ’ Žilavka’ by microsatellites and AFLP markers
Auteurs : Lidija Tomi ; Nataša Štajner ; Tatjana Jovanovi Cvetkovi ; Miljan Cvetkovi ; Branka JavornikSource :
- Acta agriculturae Slovenica [ 1581-9175 ] ; 2012-09-01.
Abstract
‘Žilavka’ has been grown in Bosnia and Herzegovina since the XIVth century and is exploited for wine production. Although not sufficiently studied, this grapevine cultivar has high economic potential for the country. Five survey missions resulted in the collection of eighty ‘Žilavka’ accessions that varied in terms of names and characteristics. Because of the unknown origin of the cultivar, these accessions were analyzed with microsatellites markers in order to obtain a standard ‘Žilavka’ genotype. AFLP markers were used to investigate the genetic basis of variability within the cultivar. ‘Žilavka’ grapevines were screened on 14 microsatellite loci, thus revealing 4 different genotypes arising from mutations observed at 10 polymorphic loci. AFLP analysis of 52 ‘Žilavka’ accessions revealed 35 different genotypes, with an average polymorphism of 57 %. Cluster analysis showed no grouping of different Žilavka accessions according to their names, characteristics or collection locations. The standard ‘Žilavka’ genotype was further compared to 211 cultivars from Slovenia (49), Austria/Germany (20), France (13), Portugal (27), Croatia (19), Greece (32), Spain (21) and Italy (30) in order to assess their genetic relationships. In pairwise comparisons, the highest genetic similarity was found with Slovenian cultivars ‘Glera’ and ‘Briška Glera’ (64 %) and the highest genetic dissimilarity (100 %) with two Italian cultivars, ‘Nebbiolo Lampia’ and ‘Vespolina’. Inventory, collection and genetic characterization of ‘Žilavka’ accessions are important steps towards cultivar standardization, identification of parental cultivars and investigation of cultivar origin, required for its sustainable use.
Url:
DOI: 10.2478/v10014-012-0014-3
Links to Exploration step
ISTEX:2B5F3D8C627EEFFBD3454EEF07984554E736107DLe document en format XML
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Natasa" uniqKey="Stajner N" first="Nataša" last="Štajner">Nataša Štajner</name><affiliation><mods:affiliation>University of Ljubljana, Biotechnical Faculty, Jamnikarjeva 101, SI-1111 Ljubljana, Slovenia</mods:affiliation></affiliation></author><author><name sortKey="Jovanovi Cvetkovi, Tatjana" sort="Jovanovi Cvetkovi, Tatjana" uniqKey="Jovanovi Cvetkovi T" first="Tatjana" last="Jovanovi Cvetkovi">Tatjana Jovanovi Cvetkovi</name><affiliation><mods:affiliation>University of Banjaluka, Faculty of Agriculture, Bulevar vojvode Petra Bojovića 1A, 78 000 Banjaluka, Bosnia and Herzegovina</mods:affiliation></affiliation></author><author><name sortKey="Cvetkovi, Miljan" sort="Cvetkovi, Miljan" uniqKey="Cvetkovi M" first="Miljan" last="Cvetkovi">Miljan Cvetkovi</name><affiliation><mods:affiliation>University of Banjaluka, Faculty of Agriculture, Bulevar vojvode Petra Bojovića 1A, 78 000 Banjaluka, Bosnia and Herzegovina</mods:affiliation></affiliation></author><author><name sortKey="Javornik, Branka" sort="Javornik, Branka" uniqKey="Javornik B" first="Branka" last="Javornik">Branka Javornik</name><affiliation><mods:affiliation>University of Ljubljana, Biotechnical Faculty, Jamnikarjeva 101, SI-1111 Ljubljana, Slovenia</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">Acta agriculturae Slovenica</title><idno type="ISSN">1581-9175</idno><idno type="eISSN">1854-1941</idno><imprint><publisher>Versita</publisher><date type="published" when="2012-09-01">2012-09-01</date><biblScope unit="volume">99</biblScope><biblScope unit="issue">2</biblScope><biblScope unit="page" from="143">143</biblScope><biblScope unit="page" to="150">150</biblScope></imprint><idno type="ISSN">1581-9175</idno></series><idno type="istex">2B5F3D8C627EEFFBD3454EEF07984554E736107D</idno><idno type="DOI">10.2478/v10014-012-0014-3</idno><idno type="ArticleID">v10014-012-0014-3</idno><idno type="pdf">v10014-012-0014-3.pdf</idno></biblStruct></sourceDesc><seriesStmt><idno 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Although not sufficiently studied, this grapevine cultivar has high economic potential for the country. Five survey missions resulted in the collection of eighty ‘Žilavka’ accessions that varied in terms of names and characteristics. Because of the unknown origin of the cultivar, these accessions were analyzed with microsatellites markers in order to obtain a standard ‘Žilavka’ genotype. AFLP markers were used to investigate the genetic basis of variability within the cultivar. ‘Žilavka’ grapevines were screened on 14 microsatellite loci, thus revealing 4 different genotypes arising from mutations observed at 10 polymorphic loci. AFLP analysis of 52 ‘Žilavka’ accessions revealed 35 different genotypes, with an average polymorphism of 57 %. Cluster analysis showed no grouping of different Žilavka accessions according to their names, characteristics or collection locations. The standard ‘Žilavka’ genotype was further compared to 211 cultivars from Slovenia (49), Austria/Germany (20), France (13), Portugal (27), Croatia (19), Greece (32), Spain (21) and Italy (30) in order to assess their genetic relationships. In pairwise comparisons, the highest genetic similarity was found with Slovenian cultivars ‘Glera’ and ‘Briška Glera’ (64 %) and the highest genetic dissimilarity (100 %) with two Italian cultivars, ‘Nebbiolo Lampia’ and ‘Vespolina’. Inventory, collection and genetic characterization of ‘Žilavka’ accessions are important steps towards cultivar standardization, identification of parental cultivars and investigation of cultivar origin, required for its sustainable use.</div></front></TEI><istex><corpusName>degruyter-journals</corpusName><author><json:item><name>Lidija Tomić</name><affiliations><json:string>University of Banjaluka, Faculty of Agriculture, Bulevar vojvode Petra Bojovića 1A, 78 000 Banjaluka, Bosnia and Herzegovina</json:string></affiliations></json:item><json:item><name>Nataša Štajner</name><affiliations><json:string>University of Ljubljana, Biotechnical Faculty, Jamnikarjeva 101, SI-1111 Ljubljana, Slovenia</json:string></affiliations></json:item><json:item><name>Tatjana Jovanović Cvetković</name><affiliations><json:string>University of Banjaluka, Faculty of Agriculture, Bulevar vojvode Petra Bojovića 1A, 78 000 Banjaluka, Bosnia and Herzegovina</json:string></affiliations></json:item><json:item><name>Miljan Cvetković</name><affiliations><json:string>University of Banjaluka, Faculty of Agriculture, Bulevar vojvode Petra Bojovića 1A, 78 000 Banjaluka, Bosnia and Herzegovina</json:string></affiliations></json:item><json:item><name>Branka Javornik</name><affiliations><json:string>University of Ljubljana, Biotechnical Faculty, Jamnikarjeva 101, SI-1111 Ljubljana, Slovenia</json:string></affiliations></json:item></author><subject><json:item><lang><json:string>eng</json:string></lang><value>grapevine</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>microsatellite</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>standard genotype</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>variability</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>AFLP</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>vinska trta</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>mikrosateliti</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>standardni genotip</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>variabilnost</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>AFLP</value></json:item></subject><articleId><json:string>v10014-012-0014-3</json:string></articleId><language><json:string>eng</json:string></language><originalGenre><json:string>research-article</json:string></originalGenre><abstract>‘Žilavka’ has been grown in Bosnia and Herzegovina since the XIVth century and is exploited for wine production. Although not sufficiently studied, this grapevine cultivar has high economic potential for the country. Five survey missions resulted in the collection of eighty ‘Žilavka’ accessions that varied in terms of names and characteristics. Because of the unknown origin of the cultivar, these accessions were analyzed with microsatellites markers in order to obtain a standard ‘Žilavka’ genotype. AFLP markers were used to investigate the genetic basis of variability within the cultivar. ‘Žilavka’ grapevines were screened on 14 microsatellite loci, thus revealing 4 different genotypes arising from mutations observed at 10 polymorphic loci. AFLP analysis of 52 ‘Žilavka’ accessions revealed 35 different genotypes, with an average polymorphism of 57 %. Cluster analysis showed no grouping of different Žilavka accessions according to their names, characteristics or collection locations. The standard ‘Žilavka’ genotype was further compared to 211 cultivars from Slovenia (49), Austria/Germany (20), France (13), Portugal (27), Croatia (19), Greece (32), Spain (21) and Italy (30) in order to assess their genetic relationships. In pairwise comparisons, the highest genetic similarity was found with Slovenian cultivars ‘Glera’ and ‘Briška Glera’ (64 %) and the highest genetic dissimilarity (100 %) with two Italian cultivars, ‘Nebbiolo Lampia’ and ‘Vespolina’. Inventory, collection and genetic characterization of ‘Žilavka’ accessions are important steps towards cultivar standardization, identification of parental cultivars and investigation of cultivar origin, required for its sustainable use.</abstract><qualityIndicators><score>8.137</score><pdfVersion>1.5</pdfVersion><pdfPageSize>595.22 x 842 pts (A4)</pdfPageSize><refBibsNative>true</refBibsNative><keywordCount>10</keywordCount><abstractCharCount>1710</abstractCharCount><pdfWordCount>3269</pdfWordCount><pdfCharCount>23494</pdfCharCount><pdfPageCount>8</pdfPageCount><abstractWordCount>239</abstractWordCount></qualityIndicators><title>Collection and genetic characterization of Vitis vinifera ’ Žilavka’ by microsatellites and AFLP markers</title><genre><json:string>research-article</json:string></genre><host><volume>99</volume><publisherId><json:string>acas</json:string></publisherId><pages><last>150</last><first>143</first></pages><issn><json:string>1581-9175</json:string></issn><issue>2</issue><genre><json:string>journal</json:string></genre><language><json:string>unknown</json:string></language><eissn><json:string>1854-1941</json:string></eissn><title>Acta agriculturae Slovenica</title></host><publicationDate>2012</publicationDate><copyrightDate>2012</copyrightDate><doi><json:string>10.2478/v10014-012-0014-3</json:string></doi><id>2B5F3D8C627EEFFBD3454EEF07984554E736107D</id><score>0.054611385</score><fulltext><json:item><extension>pdf</extension><original>true</original><mimetype>application/pdf</mimetype><uri>https://api.istex.fr/document/2B5F3D8C627EEFFBD3454EEF07984554E736107D/fulltext/pdf</uri></json:item><json:item><extension>zip</extension><original>false</original><mimetype>application/zip</mimetype><uri>https://api.istex.fr/document/2B5F3D8C627EEFFBD3454EEF07984554E736107D/fulltext/zip</uri></json:item><istex:fulltextTEI uri="https://api.istex.fr/document/2B5F3D8C627EEFFBD3454EEF07984554E736107D/fulltext/tei"><teiHeader><fileDesc><titleStmt><title level="a" type="main" xml:lang="en">Collection and genetic characterization of Vitis vinifera ’ Žilavka’ by microsatellites and AFLP markers</title></titleStmt><publicationStmt><authority>ISTEX</authority><publisher>Versita</publisher><availability status="free"><p>Open Access</p></availability><date>2012-11-20</date></publicationStmt><sourceDesc><biblStruct type="inbook"><analytic><title level="a" type="main" xml:lang="en">Collection and genetic characterization of Vitis vinifera ’ Žilavka’ by microsatellites and AFLP markers</title><author xml:id="author-1" corresp="yes"><persName><forename type="first">Lidija</forename><surname>Tomić</surname></persName><affiliation>University of Banjaluka, Faculty of Agriculture, Bulevar vojvode Petra Bojovića 1A, 78 000 Banjaluka, Bosnia and Herzegovina</affiliation></author><author xml:id="author-2"><persName><forename type="first">Nataša</forename><surname>Štajner</surname></persName><affiliation>University of Ljubljana, Biotechnical Faculty, Jamnikarjeva 101, SI-1111 Ljubljana, Slovenia</affiliation></author><author xml:id="author-3"><persName><forename type="first">Tatjana</forename><surname>Jovanović Cvetković</surname></persName><affiliation>University of Banjaluka, Faculty of Agriculture, Bulevar vojvode Petra Bojovića 1A, 78 000 Banjaluka, Bosnia and Herzegovina</affiliation></author><author xml:id="author-4"><persName><forename type="first">Miljan</forename><surname>Cvetković</surname></persName><affiliation>University of Banjaluka, Faculty of Agriculture, Bulevar vojvode Petra Bojovića 1A, 78 000 Banjaluka, Bosnia and Herzegovina</affiliation></author><author xml:id="author-5"><persName><forename type="first">Branka</forename><surname>Javornik</surname></persName><affiliation>University of Ljubljana, Biotechnical Faculty, Jamnikarjeva 101, SI-1111 Ljubljana, Slovenia</affiliation></author></analytic><monogr><title level="j">Acta agriculturae Slovenica</title><idno type="pISSN">1581-9175</idno><idno type="eISSN">1854-1941</idno><imprint><publisher>Versita</publisher><date type="published" when="2012-09-01"></date><biblScope unit="volume">99</biblScope><biblScope unit="issue">2</biblScope><biblScope unit="page" from="143">143</biblScope><biblScope unit="page" to="150">150</biblScope></imprint></monogr><idno type="istex">2B5F3D8C627EEFFBD3454EEF07984554E736107D</idno><idno type="DOI">10.2478/v10014-012-0014-3</idno><idno type="ArticleID">v10014-012-0014-3</idno><idno type="pdf">v10014-012-0014-3.pdf</idno></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>2012-11-20</date></creation><langUsage><language ident="en">en</language></langUsage><abstract xml:lang="en"><p>‘Žilavka’ has been grown in Bosnia and Herzegovina since the XIVth century and is exploited for wine production. Although not sufficiently studied, this grapevine cultivar has high economic potential for the country. Five survey missions resulted in the collection of eighty ‘Žilavka’ accessions that varied in terms of names and characteristics. Because of the unknown origin of the cultivar, these accessions were analyzed with microsatellites markers in order to obtain a standard ‘Žilavka’ genotype. AFLP markers were used to investigate the genetic basis of variability within the cultivar. ‘Žilavka’ grapevines were screened on 14 microsatellite loci, thus revealing 4 different genotypes arising from mutations observed at 10 polymorphic loci. AFLP analysis of 52 ‘Žilavka’ accessions revealed 35 different genotypes, with an average polymorphism of 57 %. Cluster analysis showed no grouping of different Žilavka accessions according to their names, characteristics or collection locations. The standard ‘Žilavka’ genotype was further compared to 211 cultivars from Slovenia (49), Austria/Germany (20), France (13), Portugal (27), Croatia (19), Greece (32), Spain (21) and Italy (30) in order to assess their genetic relationships. In pairwise comparisons, the highest genetic similarity was found with Slovenian cultivars ‘Glera’ and ‘Briška Glera’ (64 %) and the highest genetic dissimilarity (100 %) with two Italian cultivars, ‘Nebbiolo Lampia’ and ‘Vespolina’. Inventory, collection and genetic characterization of ‘Žilavka’ accessions are important steps towards cultivar standardization, identification of parental cultivars and investigation of cultivar origin, required for its sustainable use.</p></abstract><abstract xml:lang="sl"><p>‘Žilavka’ je pomembna vinska sorta z območja Bosne in Hercegovine kjer jo gojijo že od XIV stoletja. V preteklosti ni bila dovolj proučevana vendar ima velik ekonomski potencial za omenjeno območje. Akcesije žilavke (80), ki imajo različna imena in se razlikujejo po nekaterih osnovnih karakteristikah so bile nabrane na različnih področjih Bosne in Hercegovine. Za pridobitev standardnega genotipa žilavke smo uporabili mikrosatelitne markerje in z analizo 14 lokusov odkrili mutacije na 10 lokusih in določili 4 različne genotipe. Vse akcesije (52) katerim smo na osnovi mikrosatelitov določili standardni genotip smo nadalje analizirali z AFLP markerji in tako določili 35 različnih genotipov. Povprečni polimorfizem pri AFLP analizi je bil 57 %. S klastrsko analizo nismo odkrili skupin povezanih z različnimi karakteristikami, poimenovanjem ali izvorom akcesij žilavke. Standardni genotip žilavke smo nadalje primerjali z genotipi 211 kultivarjev iz Slovenije (49), Avstrije/Nemčije (20), Francije (13), Portugalske (27), Hrvaške (19), Grčije (32), Španije (21) in Italije (30), da bi ugotovili kakšna je sorodnost z njimi. V analizi primerjave parov smo ugotovili, da je žilavka najbolj sorodna (64 %) s slovenskima kultivarjema ‘Glera’ in ‘Briška Glera’ ter najbolj genetsko oddaljena od italijanskih kultivarjev ‘Nebbiolo Lampia’ in ‘Vespolina’. Zbiranje, vrednotenje in genetska karakterizacija akcesij žilavke je pomembna za standardizacijo kultivarjev, analizo starševstva in izvora sorte.</p></abstract><textClass><keywords scheme="keyword"><list><head>Keywords</head><item><term>grapevine</term></item><item><term>microsatellite</term></item><item><term>standard genotype</term></item><item><term>variability</term></item><item><term>AFLP</term></item></list></keywords></textClass><textClass xml:lang="sl"><keywords scheme="keyword"><list><head>Ključne besede</head><item><term>vinska trta</term></item><item><term>mikrosateliti</term></item><item><term>standardni genotip</term></item><item><term>variabilnost</term></item><item><term>AFLP</term></item></list></keywords></textClass></profileDesc><revisionDesc><change when="2012-11-20">Created</change><change when="2012-09-01">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/document/2B5F3D8C627EEFFBD3454EEF07984554E736107D/fulltext/txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="corpus degruyter-journals" wicri:toSee="no header"><istex:xmlDeclaration>version="1.0" encoding="UTF-8"</istex:xmlDeclaration><istex:docType PUBLIC="-//Atypon//DTD Atypon Systems Journal Archiving and Interchange NLM DTD v3.0.2 20101108//EN" URI="atypon_archive-interchange-dtd-3.0.2/atypon-archivearticle3.dtd" name="istex:docType"></istex:docType><istex:document><article article-type="research-article" dtd-version="3.0" xml:lang="EN"><front>
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<name>
<surname>Tomić</surname>
<given-names>Lidija</given-names>
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<email xlink:href="mailto:lidija.tomic@agrofabl.org">lidija.tomic@agrofabl.org</email>
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<sup>1</sup>
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<sup>2</sup>
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<surname>Jovanović Cvetković</surname>
<given-names>Tatjana</given-names>
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<sup>1</sup>
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<given-names>Miljan</given-names>
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<sup>1</sup>
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<surname>Javornik</surname>
<given-names>Branka</given-names>
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<sup>2</sup>
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<aff id="aff1"><sup>1</sup>University of Banjaluka, Faculty of Agriculture, Bulevar vojvode Petra Bojovića 1A, 78 000 Banjaluka, Bosnia and Herzegovina</aff>
<aff id="aff2"><sup>2</sup>University of Ljubljana, Biotechnical Faculty, Jamnikarjeva 101, SI-1111 Ljubljana, Slovenia</aff>
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<abstract>
<title>ABSTRACT</title>
<p> ‘Žilavka’ has been grown in Bosnia and Herzegovina since the XIVth century and is exploited for wine production. Although not sufficiently studied, this grapevine cultivar has high economic potential for the country. Five survey missions resulted in the collection of eighty ‘Žilavka’ accessions that varied in terms of names and characteristics. Because of the unknown origin of the cultivar, these accessions were analyzed with microsatellites markers in order to obtain a standard ‘Žilavka’ genotype. AFLP markers were used to investigate the genetic basis of variability within the cultivar. ‘Žilavka’ grapevines were screened on 14 microsatellite loci, thus revealing 4 different genotypes arising from mutations observed at 10 polymorphic loci. AFLP analysis of 52 ‘Žilavka’ accessions revealed 35 different genotypes, with an average polymorphism of 57 %. Cluster analysis showed no grouping of different Žilavka accessions according to their names, characteristics or collection locations. The standard ‘Žilavka’ genotype was further compared to 211 cultivars from Slovenia (49), Austria/Germany (20), France (13), Portugal (27), Croatia (19), Greece (32), Spain (21) and Italy (30) in order to assess their genetic relationships. In pairwise comparisons, the highest genetic similarity was found with Slovenian cultivars ‘Glera’ and ‘Briška Glera’ (64 %) and the highest genetic dissimilarity (100 %) with two Italian cultivars, ‘Nebbiolo Lampia’ and ‘Vespolina’. Inventory, collection and genetic characterization of ‘Žilavka’ accessions are important steps towards cultivar standardization, identification of parental cultivars and investigation of cultivar origin, required for its sustainable use.</p>
</abstract>
<abstract xml:lang="sl">
<title>IZVLEČEK</title>
<p> ‘Žilavka’ je pomembna vinska sorta z območja Bosne in Hercegovine kjer jo gojijo že od XIV stoletja. V preteklosti ni bila dovolj proučevana vendar ima velik ekonomski potencial za omenjeno območje. Akcesije žilavke (80), ki imajo različna imena in se razlikujejo po nekaterih osnovnih karakteristikah so bile nabrane na različnih področjih Bosne in Hercegovine. Za pridobitev standardnega genotipa žilavke smo uporabili mikrosatelitne markerje in z analizo 14 lokusov odkrili mutacije na 10 lokusih in določili 4 različne genotipe. Vse akcesije (52) katerim smo na osnovi mikrosatelitov določili standardni genotip smo nadalje analizirali z AFLP markerji in tako določili 35 različnih genotipov. Povprečni polimorfizem pri AFLP analizi je bil 57 %. S klastrsko analizo nismo odkrili skupin povezanih z različnimi karakteristikami, poimenovanjem ali izvorom akcesij žilavke. Standardni genotip žilavke smo nadalje primerjali z genotipi 211 kultivarjev iz Slovenije (49), Avstrije/Nemčije (20), Francije (13), Portugalske (27), Hrvaške (19), Grčije (32), Španije (21) in Italije (30), da bi ugotovili kakšna je sorodnost z njimi. V analizi primerjave parov smo ugotovili, da je žilavka najbolj sorodna (64 %) s slovenskima kultivarjema ‘Glera’ in ‘Briška Glera’ ter najbolj genetsko oddaljena od italijanskih kultivarjev ‘Nebbiolo Lampia’ in ‘Vespolina’. Zbiranje, vrednotenje in genetska karakterizacija akcesij žilavke je pomembna za standardizacijo kultivarjev, analizo starševstva in izvora sorte.</p>
</abstract>
<kwd-group>
<title>Keywords </title>
<kwd>grapevine</kwd>
<kwd>microsatellite</kwd>
<kwd>standard genotype</kwd>
<kwd>variability</kwd>
<kwd>AFLP</kwd>
</kwd-group>
<kwd-group xml:lang="sl">
<title>Ključne besede </title>
<kwd>vinska trta</kwd>
<kwd>mikrosateliti</kwd>
<kwd>standardni genotip</kwd>
<kwd>variabilnost</kwd>
<kwd>AFLP</kwd>
</kwd-group>
</article-meta>
</front></article></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Collection and genetic characterization of Vitis vinifera ’ Žilavka’ by microsatellites and AFLP markers</title></titleInfo><titleInfo type="alternative" lang="en" contentType="CDATA"><title>Collection and genetic characterization of Vitis vinifera ’ Žilavka’ by microsatellites and AFLP markers</title></titleInfo><name type="personal" displayLabel="corresp"><namePart type="given">Lidija</namePart><namePart type="family">Tomić</namePart><affiliation>University of Banjaluka, Faculty of Agriculture, Bulevar vojvode Petra Bojovića 1A, 78 000 Banjaluka, Bosnia and Herzegovina</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Nataša</namePart><namePart type="family">Štajner</namePart><affiliation>University of Ljubljana, Biotechnical Faculty, Jamnikarjeva 101, SI-1111 Ljubljana, Slovenia</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Tatjana</namePart><namePart type="family">Jovanović Cvetković</namePart><affiliation>University of Banjaluka, Faculty of Agriculture, Bulevar vojvode Petra Bojovića 1A, 78 000 Banjaluka, Bosnia and Herzegovina</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Miljan</namePart><namePart type="family">Cvetković</namePart><affiliation>University of Banjaluka, Faculty of Agriculture, Bulevar vojvode Petra Bojovića 1A, 78 000 Banjaluka, Bosnia and Herzegovina</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Branka</namePart><namePart type="family">Javornik</namePart><affiliation>University of Ljubljana, Biotechnical Faculty, Jamnikarjeva 101, SI-1111 Ljubljana, Slovenia</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="research-article"></genre><originInfo><publisher>Versita</publisher><dateIssued encoding="w3cdtf">2012-09-01</dateIssued><dateCreated encoding="w3cdtf">2012-11-20</dateCreated><copyrightDate encoding="w3cdtf">2012</copyrightDate></originInfo><language><languageTerm type="code" authority="iso639-2b">eng</languageTerm><languageTerm type="code" authority="rfc3066">en</languageTerm></language><physicalDescription><internetMediaType>text/html</internetMediaType></physicalDescription><abstract lang="en">‘Žilavka’ has been grown in Bosnia and Herzegovina since the XIVth century and is exploited for wine production. Although not sufficiently studied, this grapevine cultivar has high economic potential for the country. Five survey missions resulted in the collection of eighty ‘Žilavka’ accessions that varied in terms of names and characteristics. Because of the unknown origin of the cultivar, these accessions were analyzed with microsatellites markers in order to obtain a standard ‘Žilavka’ genotype. AFLP markers were used to investigate the genetic basis of variability within the cultivar. ‘Žilavka’ grapevines were screened on 14 microsatellite loci, thus revealing 4 different genotypes arising from mutations observed at 10 polymorphic loci. AFLP analysis of 52 ‘Žilavka’ accessions revealed 35 different genotypes, with an average polymorphism of 57 %. Cluster analysis showed no grouping of different Žilavka accessions according to their names, characteristics or collection locations. The standard ‘Žilavka’ genotype was further compared to 211 cultivars from Slovenia (49), Austria/Germany (20), France (13), Portugal (27), Croatia (19), Greece (32), Spain (21) and Italy (30) in order to assess their genetic relationships. In pairwise comparisons, the highest genetic similarity was found with Slovenian cultivars ‘Glera’ and ‘Briška Glera’ (64 %) and the highest genetic dissimilarity (100 %) with two Italian cultivars, ‘Nebbiolo Lampia’ and ‘Vespolina’. Inventory, collection and genetic characterization of ‘Žilavka’ accessions are important steps towards cultivar standardization, identification of parental cultivars and investigation of cultivar origin, required for its sustainable use.</abstract><abstract lang="sl">‘Žilavka’ je pomembna vinska sorta z območja Bosne in Hercegovine kjer jo gojijo že od XIV stoletja. V preteklosti ni bila dovolj proučevana vendar ima velik ekonomski potencial za omenjeno območje. Akcesije žilavke (80), ki imajo različna imena in se razlikujejo po nekaterih osnovnih karakteristikah so bile nabrane na različnih področjih Bosne in Hercegovine. Za pridobitev standardnega genotipa žilavke smo uporabili mikrosatelitne markerje in z analizo 14 lokusov odkrili mutacije na 10 lokusih in določili 4 različne genotipe. Vse akcesije (52) katerim smo na osnovi mikrosatelitov določili standardni genotip smo nadalje analizirali z AFLP markerji in tako določili 35 različnih genotipov. Povprečni polimorfizem pri AFLP analizi je bil 57 %. S klastrsko analizo nismo odkrili skupin povezanih z različnimi karakteristikami, poimenovanjem ali izvorom akcesij žilavke. Standardni genotip žilavke smo nadalje primerjali z genotipi 211 kultivarjev iz Slovenije (49), Avstrije/Nemčije (20), Francije (13), Portugalske (27), Hrvaške (19), Grčije (32), Španije (21) in Italije (30), da bi ugotovili kakšna je sorodnost z njimi. V analizi primerjave parov smo ugotovili, da je žilavka najbolj sorodna (64 %) s slovenskima kultivarjema ‘Glera’ in ‘Briška Glera’ ter najbolj genetsko oddaljena od italijanskih kultivarjev ‘Nebbiolo Lampia’ in ‘Vespolina’. Zbiranje, vrednotenje in genetska karakterizacija akcesij žilavke je pomembna za standardizacijo kultivarjev, analizo starševstva in izvora sorte.</abstract><subject><genre>Keywords</genre><topic>grapevine</topic><topic>microsatellite</topic><topic>standard genotype</topic><topic>variability</topic><topic>AFLP</topic></subject><subject lang="sl"><genre>Ključne besede</genre><topic>vinska trta</topic><topic>mikrosateliti</topic><topic>standardni genotip</topic><topic>variabilnost</topic><topic>AFLP</topic></subject><relatedItem type="host"><titleInfo><title>Acta agriculturae Slovenica</title></titleInfo><genre type="journal">journal</genre><identifier type="ISSN">1581-9175</identifier><identifier type="eISSN">1854-1941</identifier><identifier type="PublisherID">acas</identifier><part><date>2012</date><detail type="volume"><caption>vol.</caption><number>99</number></detail><detail type="issue"><caption>no.</caption><number>2</number></detail><extent unit="pages"><start>143</start><end>150</end></extent></part></relatedItem><identifier type="istex">2B5F3D8C627EEFFBD3454EEF07984554E736107D</identifier><identifier type="DOI">10.2478/v10014-012-0014-3</identifier><identifier type="ArticleID">v10014-012-0014-3</identifier><identifier type="pdf">v10014-012-0014-3.pdf</identifier><accessCondition type="use and reproduction" contentType="open-access">This content is open access.</accessCondition><recordInfo><recordContentSource>De Gruyter</recordContentSource></recordInfo></mods></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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