Reduction of the lenght of 1 RS.1BL translocation in the bread wheat variety "Yugoslavia"
Identifieur interne : 000553 ( Istex/Corpus ); précédent : 000552; suivant : 000554Reduction of the lenght of 1 RS.1BL translocation in the bread wheat variety "Yugoslavia"
Auteurs : Tomaž SinkoviSource :
- Acta agriculturae Slovenica [ 1581-9175 ] ; 2011-09-01.
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Abstract
This study was conducted to estimate the survival of 1RS.1BL wheat-rye translocations in a bread wheat (Triticum aestivum L. em Thell.) variety "Yugoslavia" including "Aurora" as a donor of translocation in its pedigree. The cultivar was cytologically checked by N-banding for the presence of 1RS.1BL translocation. Fluorescence in situ hybridization using total genomic biotin labelled rye DNA as a probe was used for identification and localisation of the translocation breakpoints and their size. At metaphase and interphase the introgressed rye segment was observed and photographed. Digital image analysing system with cooled CCD camera was used for overlapping the pictures of the probe signals and counterstained wheat chromosomes and helped to determine the position and extent breakpoint of this translocation. The translocation in this variety is probably restricted to the NOR region only, unlike in other 1RS.BL translocation lines ("Kavkaz") where the translocation carries at least the major part of the 1RS arm.
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DOI: 10.2478/v10014-011-0018-4
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The cultivar was cytologically checked by N-banding for the presence of 1RS.1BL translocation. Fluorescence in situ hybridization using total genomic biotin labelled rye DNA as a probe was used for identification and localisation of the translocation breakpoints and their size. At metaphase and interphase the introgressed rye segment was observed and photographed. Digital image analysing system with cooled CCD camera was used for overlapping the pictures of the probe signals and counterstained wheat chromosomes and helped to determine the position and extent breakpoint of this translocation. The translocation in this variety is probably restricted to the NOR region only, unlike in other 1RS.BL translocation lines ("Kavkaz") where the translocation carries at least the major part of the 1RS arm.</div></front></TEI><istex><corpusName>degruyter-journals</corpusName><author><json:item><name>Tomaž Sinkovič</name><affiliations><json:string>Biotechnical Faculty, University of Ljubljana, Jamnikarjeva 101, SI-1000 Ljubljana, Slovenia</json:string></affiliations></json:item></author><subject><json:item><lang><json:string>eng</json:string></lang><value>bread wheat</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Triticum aestivum L. em Thell.</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>N-banding</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>fluorescence in situ hybridization</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>1RS.1BL translocation</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>navadna pšenica</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Triticum aestivum L. em Thell.</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>N-proganje</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>flurescenčna in situ hibridizacija</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>1RS.1BL translokacija</value></json:item></subject><articleId><json:string>v10014-011-0018-4</json:string></articleId><language><json:string>eng</json:string></language><originalGenre><json:string>research-article</json:string></originalGenre><abstract>This study was conducted to estimate the survival of 1RS.1BL wheat-rye translocations in a bread wheat (Triticum aestivum L. em Thell.) variety "Yugoslavia" including "Aurora" as a donor of translocation in its pedigree. The cultivar was cytologically checked by N-banding for the presence of 1RS.1BL translocation. Fluorescence in situ hybridization using total genomic biotin labelled rye DNA as a probe was used for identification and localisation of the translocation breakpoints and their size. At metaphase and interphase the introgressed rye segment was observed and photographed. Digital image analysing system with cooled CCD camera was used for overlapping the pictures of the probe signals and counterstained wheat chromosomes and helped to determine the position and extent breakpoint of this translocation. 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The cultivar was cytologically checked by N-banding for the presence of 1RS.1BL translocation. Fluorescence in situ hybridization using total genomic biotin labelled rye DNA as a probe was used for identification and localisation of the translocation breakpoints and their size. At metaphase and interphase the introgressed rye segment was observed and photographed. Digital image analysing system with cooled CCD camera was used for overlapping the pictures of the probe signals and counterstained wheat chromosomes and helped to determine the position and extent breakpoint of this translocation. The translocation in this variety is probably restricted to the NOR region only, unlike in other 1RS.BL translocation lines ("Kavkaz") where the translocation carries at least the major part of the 1RS arm.</p></abstract><abstract xml:lang="sl"><p>Raziskava je skušala ugotoviti preživetje 1RS.1BL translokacije pri kultivarju navadne pšenice "Yugoslavia". Kultivar je imel v rodovniku "Avroro" kot donorja translokacije. Sorta je bila citološko pregledan s pomočjo N-proganja na prisotnost translokacije. Uporabili smo tudi fluorescenčno in situ hibridizacijo s celotno genomsko probo rži za natančno opredelitev mesta translokacije in dolžine. V interfazi in metafazi je bil rženi segment na kromosomu opazovan in fotografiran. Slike smo posneli z digitalno CCD kamero in sliki tarče in protibarvanih kromosomov združili z digitalno analizo slike. 1RS.1BL translokacija je bila pri tem kultivarju pšenice omejena na NOR področje kromosoma 1B, z razliko od drugih translokacij ("Kavkaz"), kjer je translokacija obsegala vsaj večji del rženega kromosomskega kraka 1RS.</p></abstract><textClass xml:lang="en"><keywords scheme="keyword"><list><head>Keywords</head><item><term>bread wheat</term></item><item><term>Triticum aestivum L. em Thell.</term></item><item><term>N-banding</term></item><item><term>fluorescence in situ hybridization</term></item><item><term>1RS.1BL translocation</term></item></list></keywords></textClass><textClass xml:lang="sl"><keywords scheme="keyword"><list><head>Keywords</head><item><term>navadna pšenica</term></item><item><term>Triticum aestivum L. em Thell.</term></item><item><term>N-proganje</term></item><item><term>flurescenčna in situ hibridizacija</term></item><item><term>1RS.1BL translokacija</term></item></list></keywords></textClass></profileDesc><revisionDesc><change when="2011-11-09">Created</change><change when="2011-09-01">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/document/5EF77ADD3BA8B07F38F4D3A7A7D5620CBD33AF19/fulltext/txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="corpus degruyter-journals" wicri:toSee="no header"><istex:xmlDeclaration>version="1.0" encoding="utf-8"</istex:xmlDeclaration><istex:docType PUBLIC="-//NLM//DTD Journal Publishing DTD v3.0 20080202//EN" URI="journalpublishing3.dtd" name="istex:docType"></istex:docType><istex:document><article article-type="research-article" dtd-version="3.0" xml:lang="en"><front><journal-meta><journal-id journal-id-type="publisher-id">ACAS</journal-id><journal-title-group><abbrev-journal-title abbrev-type="full">Acta agriculturae Slovenica</abbrev-journal-title></journal-title-group><issn pub-type="epub">1854-1941</issn><issn pub-type="ppub">1581-9175</issn><publisher><publisher-name>Versita</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="publisher-id">v10014-011-0018-4</article-id><article-id pub-id-type="doi">10.2478/v10014-011-0018-4</article-id><title-group><article-title>Reduction of the lenght of 1 RS.1BL translocation in the bread wheat variety "Yugoslavia"</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Sinkovič</surname><given-names>Tomaž</given-names></name><xref ref-type="aff" rid="A1"><sup>1</sup></xref></contrib><aff id="A1">Biotechnical Faculty, University of Ljubljana, Jamnikarjeva 101, SI-1000 Ljubljana, Slovenia<sup>1</sup></aff></contrib-group><author-notes><corresp></corresp></author-notes><pub-date pub-type="ppub"><day>1</day><month>9</month><year>2011</year></pub-date><pub-date pub-type="epub"><day>9</day><month>11</month><year>2011</year></pub-date><volume>97</volume><issue>3</issue><fpage>241</fpage><lpage>246</lpage><permissions><license license-type="open-access"><license-p>This content is open access.</license-p></license></permissions><related-article related-article-type="pdf" xlink:href="v10014-011-0018-4.pdf"></related-article><abstract xml:lang="en"><title>Reduction of the lenght of 1 RS.1BL translocation in the bread wheat variety "Yugoslavia"</title><p>This study was conducted to estimate the survival of 1RS.1BL wheat-rye translocations in a bread wheat (<italic>Triticum aestivum</italic> L. em Thell.) variety "Yugoslavia" including "Aurora" as a donor of translocation in its pedigree. The cultivar was cytologically checked by N-banding for the presence of 1RS.1BL translocation. Fluorescence <italic>in situ</italic> hybridization using total genomic biotin labelled rye DNA as a probe was used for identification and localisation of the translocation breakpoints and their size. At metaphase and interphase the introgressed rye segment was observed and photographed. Digital image analysing system with cooled CCD camera was used for overlapping the pictures of the probe signals and counterstained wheat chromosomes and helped to determine the position and extent breakpoint of this translocation. The translocation in this variety is probably restricted to the NOR region only, unlike in other 1RS.BL translocation lines ("Kavkaz") where the translocation carries at least the major part of the 1RS arm.</p></abstract><abstract xml:lang="sl"><title>Zmanjšanje Dolžine 1RS.1BL Translokacije PRI Kultivarju Navadne Pšenice "Yugoslavia"</title><p>Raziskava je skušala ugotoviti preživetje 1RS.1BL translokacije pri kultivarju navadne pšenice "Yugoslavia". Kultivar je imel v rodovniku "Avroro" kot donorja translokacije. Sorta je bila citološko pregledan s pomočjo N-proganja na prisotnost translokacije. Uporabili smo tudi fluorescenčno <italic>in situ</italic> hibridizacijo s celotno genomsko probo rži za natančno opredelitev mesta translokacije in dolžine. V interfazi in metafazi je bil rženi segment na kromosomu opazovan in fotografiran. Slike smo posneli z digitalno CCD kamero in sliki tarče in protibarvanih kromosomov združili z digitalno analizo slike. 1RS.1BL translokacija je bila pri tem kultivarju pšenice omejena na NOR področje kromosoma 1B, z razliko od drugih translokacij ("Kavkaz"), kjer je translokacija obsegala vsaj večji del rženega kromosomskega kraka 1RS.</p></abstract><kwd-group xml:lang="en"><title>Keywords</title><kwd>bread wheat</kwd><kwd><italic>Triticum aestivum</italic> L. em Thell.</kwd><kwd>N-banding</kwd><kwd>fluorescence <italic>in situ</italic> hybridization</kwd><kwd>1RS.1BL translocation</kwd></kwd-group><kwd-group xml:lang="sl"><title>Keywords</title><kwd>navadna pšenica</kwd><kwd><italic>Triticum aestivum</italic> L. em Thell.</kwd><kwd>N-proganje</kwd><kwd>flurescenčna <italic>in situ</italic> hibridizacija</kwd><kwd>1RS.1BL translokacija</kwd></kwd-group></article-meta></front></article></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Reduction of the lenght of 1 RS.1BL translocation in the bread wheat variety "Yugoslavia"</title></titleInfo><titleInfo type="alternative" lang="en" contentType="CDATA"><title>Reduction of the lenght of 1 RS.1BL translocation in the bread wheat variety "Yugoslavia"</title></titleInfo><name type="personal"><namePart type="given">Tomaž</namePart><namePart type="family">Sinkovič</namePart><affiliation>Biotechnical Faculty, University of Ljubljana, Jamnikarjeva 101, SI-1000 Ljubljana, Slovenia</affiliation></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="research-article"></genre><originInfo><publisher>Versita</publisher><dateIssued encoding="w3cdtf">2011-09-01</dateIssued><dateCreated encoding="w3cdtf">2011-11-09</dateCreated><copyrightDate encoding="w3cdtf">2011</copyrightDate></originInfo><language><languageTerm type="code" authority="iso639-2b">eng</languageTerm><languageTerm type="code" authority="rfc3066">en</languageTerm></language><physicalDescription><internetMediaType>text/html</internetMediaType></physicalDescription><abstract lang="en">This study was conducted to estimate the survival of 1RS.1BL wheat-rye translocations in a bread wheat (Triticum aestivum L. em Thell.) variety "Yugoslavia" including "Aurora" as a donor of translocation in its pedigree. The cultivar was cytologically checked by N-banding for the presence of 1RS.1BL translocation. Fluorescence in situ hybridization using total genomic biotin labelled rye DNA as a probe was used for identification and localisation of the translocation breakpoints and their size. At metaphase and interphase the introgressed rye segment was observed and photographed. Digital image analysing system with cooled CCD camera was used for overlapping the pictures of the probe signals and counterstained wheat chromosomes and helped to determine the position and extent breakpoint of this translocation. The translocation in this variety is probably restricted to the NOR region only, unlike in other 1RS.BL translocation lines ("Kavkaz") where the translocation carries at least the major part of the 1RS arm.</abstract><abstract lang="sl">Raziskava je skušala ugotoviti preživetje 1RS.1BL translokacije pri kultivarju navadne pšenice "Yugoslavia". Kultivar je imel v rodovniku "Avroro" kot donorja translokacije. Sorta je bila citološko pregledan s pomočjo N-proganja na prisotnost translokacije. Uporabili smo tudi fluorescenčno in situ hibridizacijo s celotno genomsko probo rži za natančno opredelitev mesta translokacije in dolžine. V interfazi in metafazi je bil rženi segment na kromosomu opazovan in fotografiran. Slike smo posneli z digitalno CCD kamero in sliki tarče in protibarvanih kromosomov združili z digitalno analizo slike. 1RS.1BL translokacija je bila pri tem kultivarju pšenice omejena na NOR področje kromosoma 1B, z razliko od drugih translokacij ("Kavkaz"), kjer je translokacija obsegala vsaj večji del rženega kromosomskega kraka 1RS.</abstract><subject lang="en"><genre>Keywords</genre><topic>bread wheat</topic><topic>Triticum aestivum L. em Thell.</topic><topic>N-banding</topic><topic>fluorescence in situ hybridization</topic><topic>1RS.1BL translocation</topic></subject><subject lang="sl"><genre>Keywords</genre><topic>navadna pšenica</topic><topic>Triticum aestivum L. em Thell.</topic><topic>N-proganje</topic><topic>flurescenčna in situ hibridizacija</topic><topic>1RS.1BL translokacija</topic></subject><relatedItem type="host"><titleInfo><title>Acta agriculturae Slovenica</title></titleInfo><genre type="journal">journal</genre><identifier type="ISSN">1581-9175</identifier><identifier type="eISSN">1854-1941</identifier><identifier type="PublisherID">ACAS</identifier><part><date>2011</date><detail type="volume"><caption>vol.</caption><number>97</number></detail><detail type="issue"><caption>no.</caption><number>3</number></detail><extent unit="pages"><start>241</start><end>246</end></extent></part></relatedItem><identifier type="istex">5EF77ADD3BA8B07F38F4D3A7A7D5620CBD33AF19</identifier><identifier type="DOI">10.2478/v10014-011-0018-4</identifier><identifier type="ArticleID">v10014-011-0018-4</identifier><identifier type="pdf">v10014-011-0018-4.pdf</identifier><accessCondition type="use and reproduction" contentType="open-access">This content is open access.</accessCondition><recordInfo><recordContentSource>De Gruyter</recordContentSource></recordInfo></mods></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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