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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Cloning, molecular characterization and chromosome localization of the inorganic pyrophosphatase (PPA) gene from S. cerevisiae.</title><author><name sortKey="Kolakowski, L F" sort="Kolakowski, L F" uniqKey="Kolakowski L" first="L F" last="Kolakowski">L F Kolakowski</name></author><author><name sortKey="Schloesser, M" sort="Schloesser, M" uniqKey="Schloesser M" first="M" last="Schloesser">M. Schloesser</name></author><author><name sortKey="Cooperman, B S" sort="Cooperman, B S" uniqKey="Cooperman B" first="B S" last="Cooperman">B S Cooperman</name></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">2849749</idno><idno type="pmc">338916</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC338916</idno><idno type="RBID">PMC:338916</idno><date when="1988">1988</date><idno type="wicri:Area/Pmc/Corpus">000058</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000058</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Cloning, molecular characterization and chromosome localization of the inorganic pyrophosphatase (PPA) gene from S. cerevisiae.</title><author><name sortKey="Kolakowski, L F" sort="Kolakowski, L F" uniqKey="Kolakowski L" first="L F" last="Kolakowski">L F Kolakowski</name></author><author><name sortKey="Schloesser, M" sort="Schloesser, M" uniqKey="Schloesser M" first="M" last="Schloesser">M. Schloesser</name></author><author><name sortKey="Cooperman, B S" sort="Cooperman, B S" uniqKey="Cooperman B" first="B S" last="Cooperman">B S Cooperman</name></author></analytic><series><title level="j">Nucleic Acids Research</title><idno type="ISSN">0305-1048</idno><idno type="eISSN">1362-4962</idno><imprint><date when="1988">1988</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>The gene for Saccharomyces cerevisiae inorganic pyrophosphatase, PPA, has been cloned by hybridization of "long" oligonucleotide probes with both cDNA and genomic S. cerevisiae libraries. The nucleotide sequence of 1612 bp from a genomic subclone that includes the entire coding region gives a deduced amino acid sequence that has nine differences (out of a total of 286 residues) from the previously published amino acid sequence that was determined directly. The codon usage in PPA is as expected for a "highly expressed" yeast gene. The upstream region contains a poly dA/dT sequence that might comprise a constitutive promoter. The PPA gene appears to be present in a single copy within the S. cerevisiae genome and has been localized to chromosome II.</p><sec><title>Images</title><fig id="F1"><graphic xlink:href="nar00164-0045-a" xlink:role="10444"></graphic></fig><fig id="F2"><graphic xlink:href="nar00164-0046-a" xlink:role="10445"></graphic></fig><fig id="F3"><graphic xlink:href="nar00164-0049-a" xlink:role="10448"></graphic></fig><fig id="F4"><graphic xlink:href="nar00164-0051-a" xlink:role="10450"></graphic></fig></sec></div></front></TEI><pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Nucleic Acids Res</journal-id><journal-title>Nucleic Acids Research</journal-title><issn pub-type="ppub">0305-1048</issn><issn pub-type="epub">1362-4962</issn></journal-meta><article-meta><article-id pub-id-type="pmid">2849749</article-id><article-id pub-id-type="pmc">338916</article-id><article-categories><subj-group><subject>Research Article</subject></subj-group></article-categories><title-group><article-title>Cloning, molecular characterization and chromosome localization of the inorganic pyrophosphatase (PPA) gene from S. cerevisiae.</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Kolakowski</surname><given-names>L F</given-names><suffix>Jr</suffix></name></contrib><contrib contrib-type="author"><name><surname>Schloesser</surname><given-names>M</given-names></name></contrib><contrib contrib-type="author"><name><surname>Cooperman</surname><given-names>B S</given-names></name></contrib></contrib-group><aff>University of Pennsylvania, Department of Chemistry, Philadelphia 19104.</aff><pub-date pub-type="ppub"><day>25</day><month>11</month><year>1988</year></pub-date><volume>16</volume><issue>22</issue><fpage>10441</fpage><lpage>10452</lpage><abstract><p>The gene for Saccharomyces cerevisiae inorganic pyrophosphatase, PPA, has been cloned by hybridization of "long" oligonucleotide probes with both cDNA and genomic S. cerevisiae libraries. The nucleotide sequence of 1612 bp from a genomic subclone that includes the entire coding region gives a deduced amino acid sequence that has nine differences (out of a total of 286 residues) from the previously published amino acid sequence that was determined directly. The codon usage in PPA is as expected for a "highly expressed" yeast gene. The upstream region contains a poly dA/dT sequence that might comprise a constitutive promoter. The PPA gene appears to be present in a single copy within the S. cerevisiae genome and has been localized to chromosome II.</p><sec><title>Images</title><fig id="F1"><graphic xlink:href="nar00164-0045-a" xlink:role="10444"></graphic></fig><fig id="F2"><graphic xlink:href="nar00164-0046-a" xlink:role="10445"></graphic></fig><fig id="F3"><graphic xlink:href="nar00164-0049-a" xlink:role="10448"></graphic></fig><fig id="F4"><graphic xlink:href="nar00164-0051-a" xlink:role="10450"></graphic></fig></sec></abstract></article-meta></front></pmc></record>Pour manipuler ce document sous Unix (Dilib)
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