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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Induction of proliferative responses of T cells from Babesia bovis-immune cattle with a recombinant 77-kilodalton merozoite protein (Bb-1).</title><author><name sortKey="Tetzlaff, C L" sort="Tetzlaff, C L" uniqKey="Tetzlaff C" first="C L" last="Tetzlaff">C L Tetzlaff</name></author><author><name sortKey="Rice Ficht, A C" sort="Rice Ficht, A C" uniqKey="Rice Ficht A" first="A C" last="Rice-Ficht">A C Rice-Ficht</name></author><author><name sortKey="Woods, V M" sort="Woods, V M" uniqKey="Woods V" first="V M" last="Woods">V M Woods</name></author><author><name sortKey="Brown, W C" sort="Brown, W C" uniqKey="Brown W" first="W C" last="Brown">W C Brown</name></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">1730498</idno><idno type="pmc">257678</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC257678</idno><idno type="RBID">PMC:257678</idno><date when="1992">1992</date><idno type="wicri:Area/Pmc/Corpus">000041</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000041</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Induction of proliferative responses of T cells from Babesia bovis-immune cattle with a recombinant 77-kilodalton merozoite protein (Bb-1).</title><author><name sortKey="Tetzlaff, C L" sort="Tetzlaff, C L" uniqKey="Tetzlaff C" first="C L" last="Tetzlaff">C L Tetzlaff</name></author><author><name sortKey="Rice Ficht, A C" sort="Rice Ficht, A C" uniqKey="Rice Ficht A" first="A C" last="Rice-Ficht">A C Rice-Ficht</name></author><author><name sortKey="Woods, V M" sort="Woods, V M" uniqKey="Woods V" first="V M" last="Woods">V M Woods</name></author><author><name sortKey="Brown, W C" sort="Brown, W C" uniqKey="Brown W" first="W C" last="Brown">W C Brown</name></author></analytic><series><title level="j">Infection and Immunity</title><idno type="ISSN">0019-9567</idno><idno type="eISSN">1098-5522</idno><imprint><date when="1992">1992</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>A major portion of a Babesia bovis-specific gene encoding a 77-kDa merozoite protein (Bb-1) produced during natural infection in cattle and in microaerophilous culture was subcloned into the pGEX1N expression vector. Recombinant Bb-1 protein fused to glutathione S-transferase (Bb-1-GST) was used to examine cellular immune responses in B. bovis-immune cattle. Sera from rabbits immunized with Bb-1-GST reacted with fusion protein and with the native antigen present in crude B. bovis but not with B. bigemina merozoites. Bb-1-GST but not GST induced strong proliferation of T lymphocytes from these immune cattle, and Bb-1-reactive T-cell lines which consisted of a mixed population of either CD4+ and CD8+ cells or CD4+, CD8+, and "null" (gamma delta T) cells were established by in vitro stimulation of peripheral blood mononuclear cells with the recombinant fusion protein. Three CD4+ CD8- and three CD4- CD8+ Bb-1-specific T-cell clones were identified after limiting-dilution cloning of the cell lines. The studies described here demonstrate that the 77-kDa protein of B. bovis contains T-cell epitopes capable of eliciting proliferation of two types of T cells in immune cattle, an important consideration for the design of a recombinant subunit vaccine.</p><sec sec-type="scanned-figures"><title>Images</title><fig id="F1"><graphic xlink:href="iai00026-0338-a" xlink:role="646"></graphic></fig><fig id="F2"><graphic xlink:href="iai00026-0338-b" xlink:role="646"></graphic></fig><fig id="F3"><graphic xlink:href="iai00026-0338-c" xlink:role="646"></graphic></fig><fig id="F4"><graphic xlink:href="iai00026-0339-a" xlink:role="647"></graphic></fig></sec></div></front></TEI><pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Infect Immun</journal-id><journal-title>Infection and Immunity</journal-title><issn pub-type="ppub">0019-9567</issn><issn pub-type="epub">1098-5522</issn></journal-meta><article-meta><article-id pub-id-type="pmid">1730498</article-id><article-id pub-id-type="pmc">257678</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group></article-categories><title-group><article-title>Induction of proliferative responses of T cells from Babesia bovis-immune cattle with a recombinant 77-kilodalton merozoite protein (Bb-1).</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Tetzlaff</surname><given-names>C L</given-names></name></contrib><contrib contrib-type="author"><name><surname>Rice-Ficht</surname><given-names>A C</given-names></name></contrib><contrib contrib-type="author"><name><surname>Woods</surname><given-names>V M</given-names></name></contrib><contrib contrib-type="author"><name><surname>Brown</surname><given-names>W C</given-names></name></contrib></contrib-group><aff>Department of Veterinary Pathobiology, Texas A & M University, College Station 77843.</aff><pub-date pub-type="ppub"><month>2</month><year>1992</year></pub-date><volume>60</volume><issue>2</issue><fpage>644</fpage><lpage>652</lpage><abstract><p>A major portion of a Babesia bovis-specific gene encoding a 77-kDa merozoite protein (Bb-1) produced during natural infection in cattle and in microaerophilous culture was subcloned into the pGEX1N expression vector. Recombinant Bb-1 protein fused to glutathione S-transferase (Bb-1-GST) was used to examine cellular immune responses in B. bovis-immune cattle. Sera from rabbits immunized with Bb-1-GST reacted with fusion protein and with the native antigen present in crude B. bovis but not with B. bigemina merozoites. Bb-1-GST but not GST induced strong proliferation of T lymphocytes from these immune cattle, and Bb-1-reactive T-cell lines which consisted of a mixed population of either CD4+ and CD8+ cells or CD4+, CD8+, and "null" (gamma delta T) cells were established by in vitro stimulation of peripheral blood mononuclear cells with the recombinant fusion protein. Three CD4+ CD8- and three CD4- CD8+ Bb-1-specific T-cell clones were identified after limiting-dilution cloning of the cell lines. The studies described here demonstrate that the 77-kDa protein of B. bovis contains T-cell epitopes capable of eliciting proliferation of two types of T cells in immune cattle, an important consideration for the design of a recombinant subunit vaccine.</p><sec sec-type="scanned-figures"><title>Images</title><fig id="F1"><graphic xlink:href="iai00026-0338-a" xlink:role="646"></graphic></fig><fig id="F2"><graphic xlink:href="iai00026-0338-b" xlink:role="646"></graphic></fig><fig id="F3"><graphic xlink:href="iai00026-0338-c" xlink:role="646"></graphic></fig><fig id="F4"><graphic xlink:href="iai00026-0339-a" xlink:role="647"></graphic></fig></sec></abstract></article-meta></front></pmc></record>Pour manipuler ce document sous Unix (Dilib)
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