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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Quantitative proteomic analysis of Myc oncoprotein function</title><author><name sortKey="Shiio, Yuzuru" sort="Shiio, Yuzuru" uniqKey="Shiio Y" first="Yuzuru" last="Shiio">Yuzuru Shiio</name></author><author><name sortKey="Donohoe, Sam" sort="Donohoe, Sam" uniqKey="Donohoe S" first="Sam" last="Donohoe">Sam Donohoe</name><affiliation><nlm:aff id="N0x8a24a38.0x98be200"></nlm:aff></affiliation></author><author><name sortKey="Yi, Eugene C" sort="Yi, Eugene C" uniqKey="Yi E" first="Eugene C." last="Yi">Eugene C. Yi</name><affiliation><nlm:aff id="N0x8a24a38.0x98be200"></nlm:aff></affiliation></author><author><name sortKey="Goodlett, David R" sort="Goodlett, David R" uniqKey="Goodlett D" first="David R." last="Goodlett">David R. Goodlett</name><affiliation><nlm:aff id="N0x8a24a38.0x98be200"></nlm:aff></affiliation></author><author><name sortKey="Aebersold, Ruedi" sort="Aebersold, Ruedi" uniqKey="Aebersold R" first="Ruedi" last="Aebersold">Ruedi Aebersold</name><affiliation><nlm:aff id="N0x8a24a38.0x98be200"></nlm:aff></affiliation><affiliation><nlm:aff id="N0x8a24a38.0x98be200"></nlm:aff></affiliation></author><author><name sortKey="Eisenman, Robert N" sort="Eisenman, Robert N" uniqKey="Eisenman R" first="Robert N." last="Eisenman">Robert N. Eisenman</name><affiliation><nlm:aff id="N0x8a24a38.0x98be200"></nlm:aff></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">12356725</idno><idno type="pmc">129047</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC129047</idno><idno type="RBID">PMC:129047</idno><idno type="doi">10.1093/emboj/cdf525</idno><date when="2002">2002</date><idno type="wicri:Area/Pmc/Corpus">000038</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000038</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Quantitative proteomic analysis of Myc oncoprotein function</title><author><name sortKey="Shiio, Yuzuru" sort="Shiio, Yuzuru" uniqKey="Shiio Y" first="Yuzuru" last="Shiio">Yuzuru Shiio</name></author><author><name sortKey="Donohoe, Sam" sort="Donohoe, Sam" uniqKey="Donohoe S" first="Sam" last="Donohoe">Sam Donohoe</name><affiliation><nlm:aff id="N0x8a24a38.0x98be200"></nlm:aff></affiliation></author><author><name sortKey="Yi, Eugene C" sort="Yi, Eugene C" uniqKey="Yi E" first="Eugene C." last="Yi">Eugene C. Yi</name><affiliation><nlm:aff id="N0x8a24a38.0x98be200"></nlm:aff></affiliation></author><author><name sortKey="Goodlett, David R" sort="Goodlett, David R" uniqKey="Goodlett D" first="David R." last="Goodlett">David R. Goodlett</name><affiliation><nlm:aff id="N0x8a24a38.0x98be200"></nlm:aff></affiliation></author><author><name sortKey="Aebersold, Ruedi" sort="Aebersold, Ruedi" uniqKey="Aebersold R" first="Ruedi" last="Aebersold">Ruedi Aebersold</name><affiliation><nlm:aff id="N0x8a24a38.0x98be200"></nlm:aff></affiliation><affiliation><nlm:aff id="N0x8a24a38.0x98be200"></nlm:aff></affiliation></author><author><name sortKey="Eisenman, Robert N" sort="Eisenman, Robert N" uniqKey="Eisenman R" first="Robert N." last="Eisenman">Robert N. Eisenman</name><affiliation><nlm:aff id="N0x8a24a38.0x98be200"></nlm:aff></affiliation></author></analytic><series><title level="j">The EMBO Journal</title><idno type="ISSN">0261-4189</idno><idno type="eISSN">1460-2075</idno><imprint><date when="2002">2002</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>This study applies a new quantitative proteomics technology to the analysis of the function of the Myc oncoprotein in mammalian cells. Employing isotope-coded affinity tag (ICAT<sup>TM</sup>) reagent labeling and tandem mass spectrometry, the global pattern of protein expression in rat <italic>myc</italic>-null cells was compared with that of <italic>myc</italic>-plus cells (<italic>myc</italic>-null cells in which <italic>myc</italic> has been introduced) to generate a differential protein expression catalog. Expression differences among many functionally related proteins were identified, including reduction of proteases, induction of protein synthesis pathways and upregulation of anabolic enzymes in <italic>myc</italic>-plus cells, which are predicted to lead to increased cell mass (cell growth). In addition, reduction in the levels of adhesion molecules, actin network proteins and Rho pathway proteins were observed in <italic>myc</italic>-plus cells, leading to reduced focal adhesions and actin stress fibers as well as altered morphology. These effects are dependent on the highly conserved Myc Box II region. Our results reveal a novel cytoskeletal function for Myc and indicate the feasibility of quantitative whole-proteome analysis in mammalian cells.</p></div></front></TEI><pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<front><journal-meta><journal-id journal-id-type="nlm-ta">EMBO J</journal-id><journal-title>The EMBO Journal</journal-title><issn pub-type="ppub">0261-4189</issn><issn pub-type="epub">1460-2075</issn><publisher><publisher-name>Oxford University Press</publisher-name><publisher-loc>Oxford, UK</publisher-loc></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">12356725</article-id><article-id pub-id-type="pmc">129047</article-id><article-id pub-id-type="publisher-id">cdf525</article-id><article-id pub-id-type="doi">10.1093/emboj/cdf525</article-id><article-categories><subj-group subj-group-type="heading"><subject>Articles</subject></subj-group></article-categories><title-group><article-title>Quantitative proteomic analysis of Myc oncoprotein function</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Shiio</surname><given-names>Yuzuru</given-names></name></contrib><contrib contrib-type="author"><name><surname>Donohoe</surname><given-names>Sam</given-names></name><xref ref-type="aff" rid="N0x8a24a38.0x98be200">1</xref></contrib><contrib contrib-type="author"><name><surname>Yi</surname><given-names>Eugene C.</given-names></name><xref ref-type="aff" rid="N0x8a24a38.0x98be200">1</xref></contrib><contrib contrib-type="author"><name><surname>Goodlett</surname><given-names>David R.</given-names></name><xref ref-type="aff" rid="N0x8a24a38.0x98be200">1</xref></contrib><contrib contrib-type="author"><name><surname>Aebersold</surname><given-names>Ruedi</given-names></name><xref ref-type="aff" rid="N0x8a24a38.0x98be200">1</xref><xref ref-type="aff" rid="N0x8a24a38.0x98be200">2</xref></contrib><contrib contrib-type="author"><name><surname>Eisenman</surname><given-names>Robert N.</given-names></name><xref ref-type="aff" rid="N0x8a24a38.0x98be200">2</xref></contrib></contrib-group><aff id="N0x8a24a38.0x98be200">Division of Basic Sciences, Fred Hutchinson Cancer Research Center, 1100 Fairview Avenue North, Seattle, WA 98109-1024 and<label>1</label>Institute for Systems Biology, Seattle, WA 98103, USA<label>2</label>Corresponding authors e-mail:<email>eisenman@fhcrc.org</email> or<email>raebersold@systemsbiology.org</email></aff><pub-date pub-type="ppub"><day>1</day><month>10</month><year>2002</year></pub-date><volume>21</volume><issue>19</issue><fpage>5088</fpage><lpage>5096</lpage><ext-link ext-link-type="uri" xlink:href="http://www.emboj.oupjournals.org/content/vol21/issue19/"></ext-link><history><date date-type="received"><day>17</day><month>5</month><year>2002</year></date><date date-type="rev-recd"><day>31</day><month>7</month><year>2002</year></date><date date-type="accepted"><day>14</day><month>8</month><year>2002</year></date></history><copyright-statement>Copyright © 2002 European Molecular Biology Organization</copyright-statement><copyright-year>2002</copyright-year><abstract><p>This study applies a new quantitative proteomics technology to the analysis of the function of the Myc oncoprotein in mammalian cells. Employing isotope-coded affinity tag (ICAT<sup>TM</sup>) reagent labeling and tandem mass spectrometry, the global pattern of protein expression in rat <italic>myc</italic>-null cells was compared with that of <italic>myc</italic>-plus cells (<italic>myc</italic>-null cells in which <italic>myc</italic> has been introduced) to generate a differential protein expression catalog. Expression differences among many functionally related proteins were identified, including reduction of proteases, induction of protein synthesis pathways and upregulation of anabolic enzymes in <italic>myc</italic>-plus cells, which are predicted to lead to increased cell mass (cell growth). In addition, reduction in the levels of adhesion molecules, actin network proteins and Rho pathway proteins were observed in <italic>myc</italic>-plus cells, leading to reduced focal adhesions and actin stress fibers as well as altered morphology. These effects are dependent on the highly conserved Myc Box II region. Our results reveal a novel cytoskeletal function for Myc and indicate the feasibility of quantitative whole-proteome analysis in mammalian cells.</p></abstract><kwd-group><kwd>cell growth/cytoskeleton/ICAT™ reagent/Myc oncoprotein/proteomics</kwd></kwd-group></article-meta></front></pmc></record>Pour manipuler ce document sous Unix (Dilib)
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