Uptake and degradation of 125I-labelled high density lipoproteins in rat liver cells in vivo and in vitro
Identifieur interne : 003B68 ( Main/Exploration ); précédent : 003B67; suivant : 003B69Uptake and degradation of 125I-labelled high density lipoproteins in rat liver cells in vivo and in vitro
Auteurs : Leiv Ose [Norvège] ; Turid Ose [Norvège] ; Kaare R. Norum [Norvège] ; Trond Berg [Norvège]Source :
- Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism [ 0005-2760 ] ; 1979.
Abstract
1. 1. The uptake of 125I-labelled high density lipoproteins (HDL) in various organs of the rat was determined after an intravenous injection. The uptake of 125I-labelled polyvinylpyrrolidone in the same organs was determined in order to assess uptake by fluid endocytosis. The uptake/organ was highest for the liver. The adrenals showed the highest uptake/unit weight of the organs studied. The liver, the kidneys and the spleen showed comparable values for uptake/g of tissue. The uptake of 125I-labelled HDL exceeded by far that of 125I-labelled polyvinylpyrrolidone in the liver, the kidneys, the spleen and the adrenals, indicating that the uptake of 125I-labelled HDL was mediated by adsorptive endocytosis. 2. 2. The in vivo uptake of 125I-labelled HDL was determined in purified hepatocytes and non-parenchymal cells prepared by collagenase perfusion of livers from animals after intravenous injections of 125I-labelled HDL. When expressed per cell, the hepatocytes and the non-parenchymal liver cells took up about the same amount of 125I-labelled HDL. 3. 3. The in vitro uptake and degradation of 125I-labelled HDL in isolated rat hepatocytes was studied. The uptake at increasing concentrations of 125I-labelled HDL was saturable indicating uptake mediated through binding sites. 125I-labelled HDL were easily degraded by contaminating proteases from the perfusate. 4. 4. Subcellular fractionation by isopycnic centrifugation indicated that the accumulation of 125I-labelled HDL did not take place in the lysosomes, but rather on the plasma membrane and possibly in the endosomes (phagosomes). 5. 5. 125I-labelled HDL were internalized into the cells and degraded in the lysosomes. Leupeptin and chloroquine, inhibitors of the lysosomal function effectively inhibited the formation of 125I-labelled acid-soluble radioactivity by the cells. Chloroquine, but not the protease inhibitor leupeptin, reduced the hydrolysis of the cholesteryl ester moiety of HDL.
Url:
DOI: 10.1016/0005-2760(79)90248-0
Affiliations:
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Norum</name></author><author><name sortKey="Berg, Trond" sort="Berg, Trond" uniqKey="Berg T" first="Trond" last="Berg">Trond Berg</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:FDE907CA05675D68E34DDAD529E5B25D871FB804</idno><date when="1979" year="1979">1979</date><idno type="doi">10.1016/0005-2760(79)90248-0</idno><idno type="url">https://api.istex.fr/document/FDE907CA05675D68E34DDAD529E5B25D871FB804/fulltext/pdf</idno><idno type="wicri:Area/Istex/Corpus">001F04</idno><idno type="wicri:Area/Istex/Curation">001F04</idno><idno type="wicri:Area/Istex/Checkpoint">003902</idno><idno type="wicri:doubleKey">0005-2760:1979:Ose L:uptake:and:degradation</idno><idno type="wicri:Area/Main/Merge">003C12</idno><idno type="wicri:Area/Main/Curation">003B68</idno><idno type="wicri:Area/Main/Exploration">003B68</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a">Uptake and degradation of 125I-labelled high density lipoproteins in rat liver cells in vivo and in vitro</title><author><name sortKey="Ose, Leiv" sort="Ose, Leiv" uniqKey="Ose L" first="Leiv" last="Ose">Leiv Ose</name><affiliation wicri:level="1"><country xml:lang="fr">Norvège</country><wicri:regionArea>Institute for Nutrition Research, School of Medicine, University of Oslo, Blindern, Oslo</wicri:regionArea><wicri:noRegion>Oslo</wicri:noRegion></affiliation></author><author><name sortKey="Ose, Turid" sort="Ose, Turid" uniqKey="Ose T" first="Turid" last="Ose">Turid Ose</name><affiliation wicri:level="1"><country xml:lang="fr">Norvège</country><wicri:regionArea>Institute for Nutrition Research, School of Medicine, University of Oslo, Blindern, Oslo</wicri:regionArea><wicri:noRegion>Oslo</wicri:noRegion></affiliation></author><author><name sortKey="Norum, Kaare R" sort="Norum, Kaare R" uniqKey="Norum K" first="Kaare R." last="Norum">Kaare R. Norum</name><affiliation wicri:level="1"><country xml:lang="fr">Norvège</country><wicri:regionArea>Institute for Nutrition Research, School of Medicine, University of Oslo, Blindern, Oslo</wicri:regionArea><wicri:noRegion>Oslo</wicri:noRegion></affiliation></author><author><name sortKey="Berg, Trond" sort="Berg, Trond" uniqKey="Berg T" first="Trond" last="Berg">Trond Berg</name><affiliation wicri:level="1"><country xml:lang="fr">Norvège</country><wicri:regionArea>Institute for Nutrition Research, School of Medicine, University of Oslo, Blindern, Oslo</wicri:regionArea><wicri:noRegion>Oslo</wicri:noRegion></affiliation></author></analytic><monogr></monogr><series><title level="j">Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism</title><title level="j" type="abbrev">BBALIP</title><idno type="ISSN">0005-2760</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1979">1979</date><biblScope unit="volume">574</biblScope><biblScope unit="issue">3</biblScope><biblScope unit="page" from="521">521</biblScope><biblScope unit="page" to="536">536</biblScope></imprint><idno type="ISSN">0005-2760</idno></series><idno type="istex">FDE907CA05675D68E34DDAD529E5B25D871FB804</idno><idno type="DOI">10.1016/0005-2760(79)90248-0</idno><idno type="PII">0005-2760(79)90248-0</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0005-2760</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">1. 1. The uptake of 125I-labelled high density lipoproteins (HDL) in various organs of the rat was determined after an intravenous injection. The uptake of 125I-labelled polyvinylpyrrolidone in the same organs was determined in order to assess uptake by fluid endocytosis. The uptake/organ was highest for the liver. The adrenals showed the highest uptake/unit weight of the organs studied. The liver, the kidneys and the spleen showed comparable values for uptake/g of tissue. The uptake of 125I-labelled HDL exceeded by far that of 125I-labelled polyvinylpyrrolidone in the liver, the kidneys, the spleen and the adrenals, indicating that the uptake of 125I-labelled HDL was mediated by adsorptive endocytosis. 2. 2. The in vivo uptake of 125I-labelled HDL was determined in purified hepatocytes and non-parenchymal cells prepared by collagenase perfusion of livers from animals after intravenous injections of 125I-labelled HDL. When expressed per cell, the hepatocytes and the non-parenchymal liver cells took up about the same amount of 125I-labelled HDL. 3. 3. The in vitro uptake and degradation of 125I-labelled HDL in isolated rat hepatocytes was studied. The uptake at increasing concentrations of 125I-labelled HDL was saturable indicating uptake mediated through binding sites. 125I-labelled HDL were easily degraded by contaminating proteases from the perfusate. 4. 4. Subcellular fractionation by isopycnic centrifugation indicated that the accumulation of 125I-labelled HDL did not take place in the lysosomes, but rather on the plasma membrane and possibly in the endosomes (phagosomes). 5. 5. 125I-labelled HDL were internalized into the cells and degraded in the lysosomes. Leupeptin and chloroquine, inhibitors of the lysosomal function effectively inhibited the formation of 125I-labelled acid-soluble radioactivity by the cells. Chloroquine, but not the protease inhibitor leupeptin, reduced the hydrolysis of the cholesteryl ester moiety of HDL.</div></front></TEI><affiliations><list><country><li>Norvège</li></country></list><tree><country name="Norvège"><noRegion><name sortKey="Ose, Leiv" sort="Ose, Leiv" uniqKey="Ose L" first="Leiv" last="Ose">Leiv Ose</name></noRegion><name sortKey="Berg, Trond" sort="Berg, Trond" uniqKey="Berg T" first="Trond" last="Berg">Trond Berg</name><name sortKey="Norum, Kaare R" sort="Norum, Kaare R" uniqKey="Norum K" first="Kaare R." last="Norum">Kaare R. Norum</name><name sortKey="Ose, Turid" sort="Ose, Turid" uniqKey="Ose T" first="Turid" last="Ose">Turid Ose</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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