Variables in the high-performance anion-exchange chromatography of proteins
Identifieur interne : 002F67 ( Istex/Curation ); précédent : 002F66; suivant : 002F68Variables in the high-performance anion-exchange chromatography of proteins
Auteurs : George Vanecek [États-Unis] ; Fred E. Regnier [États-Unis]Source :
- Analytical Biochemistry [ 0003-2697 ] ; 1980.
Abstract
The effect of mobile phase velocity, separation time, support pore diameter, column length, and temperature on resolution and loading capacity of a new commercially available high-performance anion-exchange support, SynChropak AX-300, has been examined. This material is a macroporous spherical silica of 10 μm particle size with a bonded polymeric amine layer. It was found that the heterogeneity of ovalbumin samples, combined with bovine serum albumin, make them useful probes in evaluation of anion-exchange supports. In the columns of 4.1 mm i.d., the highest resolutions of proteins were achieved at a flow rate of 0.25 ml/min. Up to 10 mg of protein per injection could be applied on a 4.1 × 250 mm AX-300 column with good resolution. Columns of 50 mm length had one-tenth the protein load capacity of a 250-mm column, retaining approximately 75% of the resolution.
Url:
DOI: 10.1016/0003-2697(80)90658-2
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