Cis‐acting sequences and trans‐acting factors required for constitutive expression of a microinjected HSP70 gene after the midblastula transition of Xenopus laevis embryogenesis
Identifieur interne : 003C21 ( Istex/Corpus ); précédent : 003C20; suivant : 003C22Cis‐acting sequences and trans‐acting factors required for constitutive expression of a microinjected HSP70 gene after the midblastula transition of Xenopus laevis embryogenesis
Auteurs : Nick Ovsenek ; Gregg T. Williams ; Richard I. Morimoto ; HeikkilaSource :
- Developmental Genetics [ 0192-253X ] ; 1990.
English descriptors
Abstract
Microinjected human HSP70 promoter‐chloramphenicol acetyl transferase (CAT) chimeric genes are constitutively expressed immediately after the midblastula transition of Xenopus embryogenesis. Analysis of a series of 5′‐deletion mutants in the HSP70 promoter revealed that sequences within 74 bases of the transcriptional start site were sufficient for strong basal activity. We investigated the role of specific sequences in the basal promoter by injecting HSP70‐CAT vectors containing linker‐scanner mutations in the basal elements (CCAAT, purine‐rich element, GC‐element, ATF/AP1, and 1ATA). Our data reveal that deletion of any of these cis‐acting elements in the basal promoter prevents expression after the midblastula stage of development. Furthermore, we have identified specific binding activities in embryonic nuclear extracts that complex with basal promoter elements (CCAAT, ATF, and GC) of the heterologous HSP70 promoter. These trans‐acting factors are detectable in nuclear extracts of early blastula embryos, and their respective binding activity increases dramatically after the midblastula transition. The expression of the human HSP70 gene after the midblastula transition of Xenopus embryogenesis requires an array of cisacting elements, which interact with specific Xenopus transcription factors.
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DOI: 10.1002/dvg.1020110111
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Williams</name><affiliation><mods:affiliation>Department of Biochemistry, Molecular Biology and Cell Biology, Northwestern University, Evanston, Illinois</mods:affiliation></affiliation></author><author><name sortKey="Morimoto, Richard I" sort="Morimoto, Richard I" uniqKey="Morimoto R" first="Richard I." last="Morimoto">Richard I. Morimoto</name><affiliation><mods:affiliation>Department of Biochemistry, Molecular Biology and Cell Biology, Northwestern University, Evanston, Illinois</mods:affiliation></affiliation></author><author><name sortKey="Heikkila" sort="Heikkila" uniqKey="Heikkila" last="Heikkila">Heikkila</name><affiliation><mods:affiliation>Department of Biology, University of Waterloo, Waterloo, Ontario, Canada</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">Developmental Genetics</title><title level="j" type="abbrev">Dev. Genet.</title><idno type="ISSN">0192-253X</idno><idno type="eISSN">1520-6408</idno><imprint><publisher>Wiley Subscription Services, Inc., A Wiley Company</publisher><pubPlace>Hoboken</pubPlace><date type="published" when="1990">1990</date><biblScope unit="volume">11</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="97">97</biblScope><biblScope unit="page" to="109">109</biblScope></imprint><idno type="ISSN">0192-253X</idno></series><idno type="istex">890932891C9951B34AD4599B111720FED2113C3D</idno><idno type="DOI">10.1002/dvg.1020110111</idno><idno type="ArticleID">DVG1020110111</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0192-253X</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>HSP70‐CAT</term><term>Heat shock promoters</term><term>linker‐scanner mutations</term><term>microinjection</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Microinjected human HSP70 promoter‐chloramphenicol acetyl transferase (CAT) chimeric genes are constitutively expressed immediately after the midblastula transition of Xenopus embryogenesis. Analysis of a series of 5′‐deletion mutants in the HSP70 promoter revealed that sequences within 74 bases of the transcriptional start site were sufficient for strong basal activity. We investigated the role of specific sequences in the basal promoter by injecting HSP70‐CAT vectors containing linker‐scanner mutations in the basal elements (CCAAT, purine‐rich element, GC‐element, ATF/AP1, and 1ATA). Our data reveal that deletion of any of these cis‐acting elements in the basal promoter prevents expression after the midblastula stage of development. Furthermore, we have identified specific binding activities in embryonic nuclear extracts that complex with basal promoter elements (CCAAT, ATF, and GC) of the heterologous HSP70 promoter. These trans‐acting factors are detectable in nuclear extracts of early blastula embryos, and their respective binding activity increases dramatically after the midblastula transition. The expression of the human HSP70 gene after the midblastula transition of Xenopus embryogenesis requires an array of cisacting elements, which interact with specific Xenopus transcription factors.</div></front></TEI><istex><corpusName>wiley</corpusName><author><json:item><name>Nick Ovsenek</name><affiliations><json:string>Department of Biology, University of Waterloo, Waterloo, Ontario, Canada</json:string></affiliations></json:item><json:item><name>Gregg T. Williams</name><affiliations><json:string>Department of Biochemistry, Molecular Biology and Cell Biology, Northwestern University, Evanston, Illinois</json:string></affiliations></json:item><json:item><name>Richard I. Morimoto</name><affiliations><json:string>Department of Biochemistry, Molecular Biology and Cell Biology, Northwestern University, Evanston, Illinois</json:string></affiliations></json:item><json:item><name>Dr. Heikkila</name><affiliations><json:string>Department of Biology, University of Waterloo, Waterloo, Ontario, Canada</json:string></affiliations></json:item></author><subject><json:item><lang><json:string>eng</json:string></lang><value>Heat shock promoters</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>HSP70‐CAT</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>microinjection</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>linker‐scanner mutations</value></json:item></subject><articleId><json:string>DVG1020110111</json:string></articleId><language><json:string>eng</json:string></language><abstract>Microinjected human HSP70 promoter‐chloramphenicol acetyl transferase (CAT) chimeric genes are constitutively expressed immediately after the midblastula transition of Xenopus embryogenesis. Analysis of a series of 5′‐deletion mutants in the HSP70 promoter revealed that sequences within 74 bases of the transcriptional start site were sufficient for strong basal activity. We investigated the role of specific sequences in the basal promoter by injecting HSP70‐CAT vectors containing linker‐scanner mutations in the basal elements (CCAAT, purine‐rich element, GC‐element, ATF/AP1, and 1ATA). Our data reveal that deletion of any of these cis‐acting elements in the basal promoter prevents expression after the midblastula stage of development. Furthermore, we have identified specific binding activities in embryonic nuclear extracts that complex with basal promoter elements (CCAAT, ATF, and GC) of the heterologous HSP70 promoter. These trans‐acting factors are detectable in nuclear extracts of early blastula embryos, and their respective binding activity increases dramatically after the midblastula transition. 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Genet.</title><idno type="pISSN">0192-253X</idno><idno type="eISSN">1520-6408</idno><idno type="DOI">10.1002/(ISSN)1520-6408</idno><imprint><publisher>Wiley Subscription Services, Inc., A Wiley Company</publisher><pubPlace>Hoboken</pubPlace><date type="published" when="1990"></date><biblScope unit="volume">11</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="97">97</biblScope><biblScope unit="page" to="109">109</biblScope></imprint></monogr><idno type="istex">890932891C9951B34AD4599B111720FED2113C3D</idno><idno type="DOI">10.1002/dvg.1020110111</idno><idno type="ArticleID">DVG1020110111</idno></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>1990</date></creation><langUsage><language ident="en">en</language></langUsage><abstract xml:lang="en"><p>Microinjected human HSP70 promoter‐chloramphenicol acetyl transferase (CAT) chimeric genes are constitutively expressed immediately after the midblastula transition of Xenopus embryogenesis. Analysis of a series of 5′‐deletion mutants in the HSP70 promoter revealed that sequences within 74 bases of the transcriptional start site were sufficient for strong basal activity. We investigated the role of specific sequences in the basal promoter by injecting HSP70‐CAT vectors containing linker‐scanner mutations in the basal elements (CCAAT, purine‐rich element, GC‐element, ATF/AP1, and 1ATA). Our data reveal that deletion of any of these cis‐acting elements in the basal promoter prevents expression after the midblastula stage of development. Furthermore, we have identified specific binding activities in embryonic nuclear extracts that complex with basal promoter elements (CCAAT, ATF, and GC) of the heterologous HSP70 promoter. These trans‐acting factors are detectable in nuclear extracts of early blastula embryos, and their respective binding activity increases dramatically after the midblastula transition. The expression of the human HSP70 gene after the midblastula transition of Xenopus embryogenesis requires an array of cisacting elements, which interact with specific Xenopus transcription factors.</p></abstract><textClass xml:lang="en"><keywords scheme="keyword"><list><head>Keywords</head><item><term>Heat shock promoters</term></item><item><term>HSP70‐CAT</term></item><item><term>microinjection</term></item><item><term>linker‐scanner mutations</term></item></list></keywords></textClass><textClass><keywords scheme="Journal Subject"><list><head>article category</head><item><term>Article</term></item></list></keywords></textClass></profileDesc><revisionDesc><change when="1989-11-03">Received</change><change when="1989-12-22">Registration</change><change when="1990">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><original>false</original><mimetype>text/plain</mimetype><extension>txt</extension><uri>https://api.istex.fr/document/890932891C9951B34AD4599B111720FED2113C3D/fulltext/txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Wiley, elements deleted: body"><istex:xmlDeclaration>version="1.0" encoding="UTF-8" standalone="yes"</istex:xmlDeclaration><istex:document><component version="2.0" type="serialArticle" xml:lang="en"><header><publicationMeta level="product"><publisherInfo><publisherName>Wiley Subscription Services, Inc., A Wiley Company</publisherName><publisherLoc>Hoboken</publisherLoc></publisherInfo><doi registered="yes">10.1002/(ISSN)1520-6408</doi><issn type="print">0192-253X</issn><issn type="electronic">1520-6408</issn><idGroup><id type="product" value="DVG"></id></idGroup><titleGroup><title type="main" xml:lang="en" sort="DEVELOPMENTAL GENETICS">Developmental Genetics</title><title type="short">Dev. 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Analysis of a series of 5′‐deletion mutants in the HSP70 promoter revealed that sequences within 74 bases of the transcriptional start site were sufficient for strong basal activity. We investigated the role of specific sequences in the basal promoter by injecting HSP70‐CAT vectors containing linker‐scanner mutations in the basal elements (CCAAT, purine‐rich element, GC‐element, ATF/AP1, and 1ATA). Our data reveal that deletion of any of these cis‐acting elements in the basal promoter prevents expression after the midblastula stage of development. Furthermore, we have identified specific binding activities in embryonic nuclear extracts that complex with basal promoter elements (CCAAT, ATF, and GC) of the heterologous HSP70 promoter. These trans‐acting factors are detectable in nuclear extracts of early blastula embryos, and their respective binding activity increases dramatically after the midblastula transition. The expression of the human HSP70 gene after the midblastula transition of <i>Xenopus</i> embryogenesis requires an array of cisacting elements, which interact with specific <i>Xenopus</i> transcription factors.</p></abstract></abstractGroup></contentMeta></header></component></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Cis‐acting sequences and trans‐acting factors required for constitutive expression of a microinjected HSP70 gene after the midblastula transition of Xenopus laevis embryogenesis</title></titleInfo><titleInfo type="abbreviated" lang="en"><title>HSP70 GENE EXPRESSION DURING EMBRYOGENESIS</title></titleInfo><titleInfo type="alternative" contentType="CDATA" lang="en"><title>Cis‐acting sequences and trans‐acting factors required for constitutive expression of a microinjected HSP70 gene after the midblastula transition of</title></titleInfo><name type="personal"><namePart type="given">Nick</namePart><namePart type="family">Ovsenek</namePart><affiliation>Department of Biology, University of Waterloo, Waterloo, Ontario, Canada</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Gregg T.</namePart><namePart type="family">Williams</namePart><affiliation>Department of Biochemistry, Molecular Biology and Cell Biology, Northwestern University, Evanston, Illinois</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Richard I.</namePart><namePart type="family">Morimoto</namePart><affiliation>Department of Biochemistry, Molecular Biology and Cell Biology, Northwestern University, Evanston, Illinois</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="termsOfAddress">Dr.</namePart><namePart type="family">Heikkila</namePart><affiliation>Department of Biology, University of Waterloo, Waterloo, Ontario, Canada</affiliation><description>Correspondence: Department of Biology, University of Waterloo, Waterloo, Ontario, N2L 3G1 Canada</description><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="article" displayLabel="article"></genre><originInfo><publisher>Wiley Subscription Services, Inc., A Wiley Company</publisher><place><placeTerm type="text">Hoboken</placeTerm></place><dateIssued encoding="w3cdtf">1990</dateIssued><dateCaptured encoding="w3cdtf">1989-11-03</dateCaptured><dateValid encoding="w3cdtf">1989-12-22</dateValid><copyrightDate encoding="w3cdtf">1990</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><physicalDescription><internetMediaType>text/html</internetMediaType><extent unit="figures">9</extent><extent unit="references">71</extent></physicalDescription><abstract lang="en">Microinjected human HSP70 promoter‐chloramphenicol acetyl transferase (CAT) chimeric genes are constitutively expressed immediately after the midblastula transition of Xenopus embryogenesis. Analysis of a series of 5′‐deletion mutants in the HSP70 promoter revealed that sequences within 74 bases of the transcriptional start site were sufficient for strong basal activity. We investigated the role of specific sequences in the basal promoter by injecting HSP70‐CAT vectors containing linker‐scanner mutations in the basal elements (CCAAT, purine‐rich element, GC‐element, ATF/AP1, and 1ATA). Our data reveal that deletion of any of these cis‐acting elements in the basal promoter prevents expression after the midblastula stage of development. Furthermore, we have identified specific binding activities in embryonic nuclear extracts that complex with basal promoter elements (CCAAT, ATF, and GC) of the heterologous HSP70 promoter. These trans‐acting factors are detectable in nuclear extracts of early blastula embryos, and their respective binding activity increases dramatically after the midblastula transition. The expression of the human HSP70 gene after the midblastula transition of Xenopus embryogenesis requires an array of cisacting elements, which interact with specific Xenopus transcription factors.</abstract><subject lang="en"><genre>Keywords</genre><topic>Heat shock promoters</topic><topic>HSP70‐CAT</topic><topic>microinjection</topic><topic>linker‐scanner mutations</topic></subject><relatedItem type="host"><titleInfo><title>Developmental Genetics</title></titleInfo><titleInfo type="abbreviated"><title>Dev. Genet.</title></titleInfo><genre type="Journal">journal</genre><subject><genre>article category</genre><topic>Article</topic></subject><identifier type="ISSN">0192-253X</identifier><identifier type="eISSN">1520-6408</identifier><identifier type="DOI">10.1002/(ISSN)1520-6408</identifier><identifier type="PublisherID">DVG</identifier><part><date>1990</date><detail type="volume"><caption>vol.</caption><number>11</number></detail><detail type="issue"><caption>no.</caption><number>1</number></detail><extent unit="pages"><start>97</start><end>109</end><total>13</total></extent></part></relatedItem><identifier type="istex">890932891C9951B34AD4599B111720FED2113C3D</identifier><identifier type="DOI">10.1002/dvg.1020110111</identifier><identifier type="ArticleID">DVG1020110111</identifier><accessCondition type="use and reproduction" contentType="copyright">Copyright © 1990 Wiley‐Liss, Inc.</accessCondition><recordInfo><recordContentSource>WILEY</recordContentSource><recordOrigin>Wiley Subscription Services, Inc., A Wiley Company</recordOrigin></recordInfo></mods></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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