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Structure of egg yolk very low density lipoprotein. Polydispersity of the very low density lipoprotein and the role of lipovitellenin in the structure

Identifieur interne : 002C08 ( Istex/Corpus ); précédent : 002C07; suivant : 002C09

Structure of egg yolk very low density lipoprotein. Polydispersity of the very low density lipoprotein and the role of lipovitellenin in the structure

Auteurs : Robert John Evans ; Doris H. Bauer ; Selma L. Bandemer ; Simin B. Vaghefi ; Cal J. Flegal

Source :

RBID : ISTEX:46E6157F93AF1CF86C5C74B596D01C82F4FAB6CA

Abstract

Egg yolk very low density lipoprotein contained on the average 75% of lipid which could be extracted by ether and 25% of a residual lipoprotein, the classical lipovitellenin. The ether-extracted lipid was composed of 75% triglycerides, 7% sterols, 2% mono- and diglycerides, and 16% phospholipids. Lipovitellenin contained 48% lipid composed of 87% phospholipids, 11% triglycerides, and 2% sterols. The protein vitellenin was composed for the most part of units of 74,000 and 270,000 daltons molecular weight.Egg yolk very low density lipoprotein is polydisperse. Preparative ultracentrifugation separated it into six fractions of different average molecular size, and gel chromatography separated it into five. The fractions of larger molecular size contained more lipid and triglyceride than did the fractions of smaller molecular size. The proteins of the fractions appeared to be similar.Egg yolk very low density lipoproteins appear to be a series of molecules composed of cores of lipid of varying sizes with each core surrounded by a layer of lipovitellenin, which is composed principally of glycoprotein and phospholipid.

Url:
DOI: 10.1016/0003-9861(73)90001-5

Links to Exploration step

ISTEX:46E6157F93AF1CF86C5C74B596D01C82F4FAB6CA

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<div type="abstract" xml:lang="en">Egg yolk very low density lipoprotein contained on the average 75% of lipid which could be extracted by ether and 25% of a residual lipoprotein, the classical lipovitellenin. The ether-extracted lipid was composed of 75% triglycerides, 7% sterols, 2% mono- and diglycerides, and 16% phospholipids. Lipovitellenin contained 48% lipid composed of 87% phospholipids, 11% triglycerides, and 2% sterols. The protein vitellenin was composed for the most part of units of 74,000 and 270,000 daltons molecular weight.Egg yolk very low density lipoprotein is polydisperse. Preparative ultracentrifugation separated it into six fractions of different average molecular size, and gel chromatography separated it into five. The fractions of larger molecular size contained more lipid and triglyceride than did the fractions of smaller molecular size. The proteins of the fractions appeared to be similar.Egg yolk very low density lipoproteins appear to be a series of molecules composed of cores of lipid of varying sizes with each core surrounded by a layer of lipovitellenin, which is composed principally of glycoprotein and phospholipid.</div>
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<dateIssued encoding="w3cdtf">1972</dateIssued>
<dateCaptured encoding="w3cdtf">1972-08-07</dateCaptured>
<copyrightDate encoding="w3cdtf">1973</copyrightDate>
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<languageTerm type="code" authority="iso639-2b">eng</languageTerm>
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<abstract lang="en">Egg yolk very low density lipoprotein contained on the average 75% of lipid which could be extracted by ether and 25% of a residual lipoprotein, the classical lipovitellenin. The ether-extracted lipid was composed of 75% triglycerides, 7% sterols, 2% mono- and diglycerides, and 16% phospholipids. Lipovitellenin contained 48% lipid composed of 87% phospholipids, 11% triglycerides, and 2% sterols. The protein vitellenin was composed for the most part of units of 74,000 and 270,000 daltons molecular weight.Egg yolk very low density lipoprotein is polydisperse. Preparative ultracentrifugation separated it into six fractions of different average molecular size, and gel chromatography separated it into five. The fractions of larger molecular size contained more lipid and triglyceride than did the fractions of smaller molecular size. The proteins of the fractions appeared to be similar.Egg yolk very low density lipoproteins appear to be a series of molecules composed of cores of lipid of varying sizes with each core surrounded by a layer of lipovitellenin, which is composed principally of glycoprotein and phospholipid.</abstract>
<note>Michigan Agricultural Experiment Station Journal Article No. 6032. This investigation was supported in part by Public Health Service Research Grant AM 10214 from the National Institute of Arthritis and Metabolic Diseases.</note>
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<topic>Lipids</topic>
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<originInfo>
<dateIssued encoding="w3cdtf">197302</dateIssued>
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<identifier type="ISSN">0003-9861</identifier>
<identifier type="PII">S0003-9861(00)X0280-9</identifier>
<part>
<date>197302</date>
<detail type="volume">
<number>154</number>
<caption>vol.</caption>
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<detail type="issue">
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<caption>no.</caption>
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<end>727</end>
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<end>500</end>
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<identifier type="DOI">10.1016/0003-9861(73)90001-5</identifier>
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