Determination of proteins by amperometric titration with 12-phosphotungstic acid at rotating gold electrodes
Identifieur interne : 000D27 ( Istex/Corpus ); précédent : 000D26; suivant : 000D28Determination of proteins by amperometric titration with 12-phosphotungstic acid at rotating gold electrodes
Auteurs : Stephen R. Betso ; Peter W. CarrSource :
- Analytica Chimica Acta [ 0003-2670 ] ; 1973.
Abstract
Amperometric titration of various protein fractions and human serum with 12-phosphotungstic acid at the rotating gold electrode is described. The titrant is electroreducible and its concentration may be monitored by poising an amperometric indicator electrode at a potential of -0.25 V vs. SCE. In 1.0 M hydrochloric acid, well defined titration curves of proteins may be obtained in the presence of airsaturated solutions. The relative standard deviation of replicate samples is 0.2–0.3% in the range 2–4 g of protein per 1 in the sample solution. The technique is principally applicable to homogeneous mixtures of proteins.
Url:
DOI: 10.1016/0003-2670(74)80019-X
Links to Exploration step
ISTEX:52C943B61503F54CCE63D28D68A60A5EB1A6AE20Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Determination of proteins by amperometric titration with 12-phosphotungstic acid at rotating gold electrodes</title><author><name sortKey="Betso, Stephen R" sort="Betso, Stephen R" uniqKey="Betso S" first="Stephen R." last="Betso">Stephen R. Betso</name><affiliation><mods:affiliation>Department of Chemistry, University of Georgia, Athens, Ga. 30602 U.S.A.</mods:affiliation></affiliation></author><author><name sortKey="Carr, Peter W" sort="Carr, Peter W" uniqKey="Carr P" first="Peter W." last="Carr">Peter W. Carr</name><affiliation><mods:affiliation>Department of Chemistry, University of Georgia, Athens, Ga. 30602 U.S.A.</mods:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:52C943B61503F54CCE63D28D68A60A5EB1A6AE20</idno><date when="1974" year="1974">1974</date><idno type="doi">10.1016/0003-2670(74)80019-X</idno><idno type="url">https://api.istex.fr/document/52C943B61503F54CCE63D28D68A60A5EB1A6AE20/fulltext/pdf</idno><idno type="wicri:Area/Istex/Corpus">000D27</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Determination of proteins by amperometric titration with 12-phosphotungstic acid at rotating gold electrodes</title><author><name sortKey="Betso, Stephen R" sort="Betso, Stephen R" uniqKey="Betso S" first="Stephen R." last="Betso">Stephen R. Betso</name><affiliation><mods:affiliation>Department of Chemistry, University of Georgia, Athens, Ga. 30602 U.S.A.</mods:affiliation></affiliation></author><author><name sortKey="Carr, Peter W" sort="Carr, Peter W" uniqKey="Carr P" first="Peter W." last="Carr">Peter W. Carr</name><affiliation><mods:affiliation>Department of Chemistry, University of Georgia, Athens, Ga. 30602 U.S.A.</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">Analytica Chimica Acta</title><title level="j" type="abbrev">ACA</title><idno type="ISSN">0003-2670</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1973">1973</date><biblScope unit="volume">69</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="161">161</biblScope><biblScope unit="page" to="172">172</biblScope></imprint><idno type="ISSN">0003-2670</idno></series><idno type="istex">52C943B61503F54CCE63D28D68A60A5EB1A6AE20</idno><idno type="DOI">10.1016/0003-2670(74)80019-X</idno><idno type="PII">0003-2670(74)80019-X</idno><idno type="ArticleID">7480019X</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0003-2670</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Amperometric titration of various protein fractions and human serum with 12-phosphotungstic acid at the rotating gold electrode is described. The titrant is electroreducible and its concentration may be monitored by poising an amperometric indicator electrode at a potential of -0.25 V vs. SCE. In 1.0 M hydrochloric acid, well defined titration curves of proteins may be obtained in the presence of airsaturated solutions. The relative standard deviation of replicate samples is 0.2–0.3% in the range 2–4 g of protein per 1 in the sample solution. The technique is principally applicable to homogeneous mixtures of proteins.</div></front></TEI><istex><corpusName>elsevier</corpusName><author><json:item><name>Stephen R. Betso</name><affiliations><json:string>Department of Chemistry, University of Georgia, Athens, Ga. 30602 U.S.A.</json:string></affiliations></json:item><json:item><name>Peter W. Carr</name><affiliations><json:string>Department of Chemistry, University of Georgia, Athens, Ga. 30602 U.S.A.</json:string></affiliations></json:item></author><articleId><json:string>7480019X</json:string></articleId><language><json:string>eng</json:string></language><qualityIndicators><score>4.925</score><pdfVersion>1.2</pdfVersion><pdfPageSize>576 x 720 pts</pdfPageSize><refBibsNative>true</refBibsNative><keywordCount>0</keywordCount><abstractCharCount>625</abstractCharCount><pdfWordCount>3785</pdfWordCount><pdfCharCount>26347</pdfCharCount><pdfPageCount>12</pdfPageCount><abstractWordCount>95</abstractWordCount></qualityIndicators><title>Determination of proteins by amperometric titration with 12-phosphotungstic acid at rotating gold electrodes</title><pii><json:string>0003-2670(74)80019-X</json:string></pii><genre><json:string>research-article</json:string></genre><serie><genre></genre><language><json:string>unknown</json:string></language><title>Chapter 5</title></serie><host><volume>69</volume><pii><json:string>S0003-2670(00)X0613-7</json:string></pii><pages><last>172</last><first>161</first></pages><issn><json:string>0003-2670</json:string></issn><issue>1</issue><genre><json:string>Journal</json:string></genre><language><json:string>unknown</json:string></language><title>Analytica Chimica Acta</title><publicationDate>1974</publicationDate></host><publicationDate>1973</publicationDate><copyrightDate>1974</copyrightDate><doi><json:string>10.1016/0003-2670(74)80019-X</json:string></doi><id>52C943B61503F54CCE63D28D68A60A5EB1A6AE20</id><fulltext><json:item><original>true</original><mimetype>application/pdf</mimetype><extension>pdf</extension><uri>https://api.istex.fr/document/52C943B61503F54CCE63D28D68A60A5EB1A6AE20/fulltext/pdf</uri></json:item><json:item><original>true</original><mimetype>text/plain</mimetype><extension>txt</extension><uri>https://api.istex.fr/document/52C943B61503F54CCE63D28D68A60A5EB1A6AE20/fulltext/txt</uri></json:item><json:item><original>false</original><mimetype>application/zip</mimetype><extension>zip</extension><uri>https://api.istex.fr/document/52C943B61503F54CCE63D28D68A60A5EB1A6AE20/fulltext/zip</uri></json:item><istex:fulltextTEI uri="https://api.istex.fr/document/52C943B61503F54CCE63D28D68A60A5EB1A6AE20/fulltext/tei"><teiHeader><fileDesc><titleStmt><title level="a" type="main" xml:lang="en">Determination of proteins by amperometric titration with 12-phosphotungstic acid at rotating gold electrodes</title></titleStmt><publicationStmt><authority>ISTEX</authority><publisher>ELSEVIER</publisher><availability><p>ELSEVIER</p></availability><date>1974</date></publicationStmt><sourceDesc><biblStruct type="inbook"><analytic><title level="a" type="main" xml:lang="en">Determination of proteins by amperometric titration with 12-phosphotungstic acid at rotating gold electrodes</title><author><persName><forename type="first">Stephen R.</forename><surname>Betso</surname></persName><affiliation>Department of Chemistry, University of Georgia, Athens, Ga. 30602 U.S.A.</affiliation></author><author><persName><forename type="first">Peter W.</forename><surname>Carr</surname></persName><affiliation>Department of Chemistry, University of Georgia, Athens, Ga. 30602 U.S.A.</affiliation></author></analytic><monogr><title level="j">Analytica Chimica Acta</title><title level="j" type="abbrev">ACA</title><idno type="pISSN">0003-2670</idno><idno type="PII">S0003-2670(00)X0613-7</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1973"></date><biblScope unit="volume">69</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="161">161</biblScope><biblScope unit="page" to="172">172</biblScope></imprint></monogr><idno type="istex">52C943B61503F54CCE63D28D68A60A5EB1A6AE20</idno><idno type="DOI">10.1016/0003-2670(74)80019-X</idno><idno type="PII">0003-2670(74)80019-X</idno><idno type="ArticleID">7480019X</idno></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>1974</date></creation><langUsage><language ident="en">en</language></langUsage><abstract xml:lang="en"><p>Amperometric titration of various protein fractions and human serum with 12-phosphotungstic acid at the rotating gold electrode is described. The titrant is electroreducible and its concentration may be monitored by poising an amperometric indicator electrode at a potential of -0.25 V vs. SCE. In 1.0 M hydrochloric acid, well defined titration curves of proteins may be obtained in the presence of airsaturated solutions. The relative standard deviation of replicate samples is 0.2–0.3% in the range 2–4 g of protein per 1 in the sample solution. The technique is principally applicable to homogeneous mixtures of proteins.</p></abstract><abstract xml:lang="fr"><p>Un titrage ampérométrique avec électrode d'or tournante est décrit pour le dosage de diverses fractions de protéïne et de sérum humains, au moyen d'acide 12-phosphotungstique. Le titrant est électroréductible; sa concentration peut être contrôlée en maintenant l'électrode ampérométrique indicatrice à un potentiel de -0.25 V vs. ECS. En milieu acide chlorhydrique 1.0 M, des courbes de titrage bien définies de protéïnes peuvent être obtenues, en présence de solutions saturées d'air. La déviation standard relative est de 0.2–0.3% pour des concentrations de l'ordre de 2–4 g de protéïne par litre. Cette technique est principalement applicable à des mélanges homogènes de protéïnes.</p></abstract><abstract xml:lang="de"><p>Es wird die amperometrische Titration von verschiedenen Proteinfraktionen und menschlichem Serum mit 12-Phosphorwolframsäure an der rotierenden Goldelektrode beschrieben. Das Reagenz ist elektrochemisch reduzierbar, und dessen Konzentration kann kontrolliert werden, indem eine amperometrische Indikatorelektrode auf einem Potential von -0.25 V gegen ges. Kalomeleleklrode gehalten wird. In 1.0 M Salzsäure können gut ausgebildete Titrationskurven von Proteinen in Gegenwart luftgesättigter Lösungen erhalten werden. Die relative Standardabweichung im Bereich 2–4 g Protein pro 1 Probelösung ist 0.2–0.3%. Das Verfahren ist prinzipiell auf homogene Gemische von Proteinen anwendbar.</p></abstract></profileDesc><revisionDesc><change when="1973-05-03">Received</change><change when="1973">Published</change></revisionDesc></teiHeader></istex:fulltextTEI></fulltext><metadata><istex:metadataXml wicri:clean="Elsevier, elements deleted: tail"><istex:xmlDeclaration>version="1.0" encoding="UTF-8"</istex:xmlDeclaration><istex:docType PUBLIC="-//ES//DTD journal article DTD version 4.5.2//EN//XML" URI="art452.dtd" name="istex:docType"></istex:docType><istex:document><converted-article version="4.5.2" docsubtype="fla" xml:lang="en"><item-info><jid>ACA</jid><aid>7480019X</aid><ce:pii>0003-2670(74)80019-X</ce:pii><ce:doi>10.1016/0003-2670(74)80019-X</ce:doi><ce:copyright type="unknown" year="1974"></ce:copyright></item-info><head><ce:title>Determination of proteins by amperometric titration with 12-phosphotungstic acid at rotating gold electrodes</ce:title><ce:author-group><ce:author><ce:given-name>Stephen R.</ce:given-name><ce:surname>Betso</ce:surname></ce:author><ce:author><ce:given-name>Peter W.</ce:given-name><ce:surname>Carr</ce:surname></ce:author><ce:affiliation><ce:textfn>Department of Chemistry, University of Georgia, Athens, Ga. 30602 U.S.A.</ce:textfn></ce:affiliation></ce:author-group><ce:date-received day="3" month="5" year="1973"></ce:date-received><ce:abstract class="author"><ce:section-title>Abstract</ce:section-title><ce:abstract-sec><ce:simple-para view="all" id="simple-para.0010">Amperometric titration of various protein fractions and human serum with 12-phosphotungstic acid at the rotating gold electrode is described. The titrant is electroreducible and its concentration may be monitored by poising an amperometric indicator electrode at a potential of -0.25 V <ce:italic>vs.</ce:italic> SCE. In 1.0 <ce:italic>M</ce:italic> hydrochloric acid, well defined titration curves of proteins may be obtained in the presence of airsaturated solutions. The relative standard deviation of replicate samples is 0.2–0.3% in the range 2–4 g of protein per 1 in the sample solution. The technique is principally applicable to homogeneous mixtures of proteins.</ce:simple-para></ce:abstract-sec></ce:abstract><ce:abstract xml:lang="fr" class="author"><ce:section-title>Résumé</ce:section-title><ce:abstract-sec><ce:simple-para view="all" id="simple-para.0015">Un titrage ampérométrique avec électrode d'or tournante est décrit pour le dosage de diverses fractions de protéïne et de sérum humains, au moyen d'acide 12-phosphotungstique. Le titrant est électroréductible; sa concentration peut être contrôlée en maintenant l'électrode ampérométrique indicatrice à un potentiel de -0.25 V <ce:italic>vs.</ce:italic> ECS. En milieu acide chlorhydrique 1.0 <ce:italic>M</ce:italic>, des courbes de titrage bien définies de protéïnes peuvent être obtenues, en présence de solutions saturées d'air. La déviation standard relative est de 0.2–0.3% pour des concentrations de l'ordre de 2–4 g de protéïne par litre. Cette technique est principalement applicable à des mélanges homogènes de protéïnes.</ce:simple-para></ce:abstract-sec></ce:abstract><ce:abstract xml:lang="de" class="author"><ce:section-title>Zusammenfassung</ce:section-title><ce:abstract-sec><ce:simple-para view="all" id="simple-para.0020">Es wird die amperometrische Titration von verschiedenen Proteinfraktionen und menschlichem Serum mit 12-Phosphorwolframsäure an der rotierenden Goldelektrode beschrieben. Das Reagenz ist elektrochemisch reduzierbar, und dessen Konzentration kann kontrolliert werden, indem eine amperometrische Indikatorelektrode auf einem Potential von -0.25 V gegen ges. Kalomeleleklrode gehalten wird. In 1.0 <ce:italic>M</ce:italic> Salzsäure können gut ausgebildete Titrationskurven von Proteinen in Gegenwart luftgesättigter Lösungen erhalten werden. Die relative Standardabweichung im Bereich 2–4 g Protein pro 1 Probelösung ist 0.2–0.3%. Das Verfahren ist prinzipiell auf homogene Gemische von Proteinen anwendbar.</ce:simple-para></ce:abstract-sec></ce:abstract></head></converted-article></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Determination of proteins by amperometric titration with 12-phosphotungstic acid at rotating gold electrodes</title></titleInfo><titleInfo type="alternative" lang="en" contentType="CDATA"><title>Determination of proteins by amperometric titration with 12-phosphotungstic acid at rotating gold electrodes</title></titleInfo><name type="personal"><namePart type="given">Stephen R.</namePart><namePart type="family">Betso</namePart><affiliation>Department of Chemistry, University of Georgia, Athens, Ga. 30602 U.S.A.</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Peter W.</namePart><namePart type="family">Carr</namePart><affiliation>Department of Chemistry, University of Georgia, Athens, Ga. 30602 U.S.A.</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="Full-length article"></genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">1973</dateIssued><dateCaptured encoding="w3cdtf">1973-05-03</dateCaptured><copyrightDate encoding="w3cdtf">1974</copyrightDate></originInfo><language><languageTerm type="code" authority="iso639-2b">eng</languageTerm><languageTerm type="code" authority="rfc3066">en</languageTerm></language><physicalDescription><internetMediaType>text/html</internetMediaType></physicalDescription><abstract lang="en">Amperometric titration of various protein fractions and human serum with 12-phosphotungstic acid at the rotating gold electrode is described. The titrant is electroreducible and its concentration may be monitored by poising an amperometric indicator electrode at a potential of -0.25 V vs. SCE. In 1.0 M hydrochloric acid, well defined titration curves of proteins may be obtained in the presence of airsaturated solutions. The relative standard deviation of replicate samples is 0.2–0.3% in the range 2–4 g of protein per 1 in the sample solution. The technique is principally applicable to homogeneous mixtures of proteins.</abstract><abstract lang="fr">Un titrage ampérométrique avec électrode d'or tournante est décrit pour le dosage de diverses fractions de protéïne et de sérum humains, au moyen d'acide 12-phosphotungstique. Le titrant est électroréductible; sa concentration peut être contrôlée en maintenant l'électrode ampérométrique indicatrice à un potentiel de -0.25 V vs. ECS. En milieu acide chlorhydrique 1.0 M, des courbes de titrage bien définies de protéïnes peuvent être obtenues, en présence de solutions saturées d'air. La déviation standard relative est de 0.2–0.3% pour des concentrations de l'ordre de 2–4 g de protéïne par litre. Cette technique est principalement applicable à des mélanges homogènes de protéïnes.</abstract><abstract lang="de">Es wird die amperometrische Titration von verschiedenen Proteinfraktionen und menschlichem Serum mit 12-Phosphorwolframsäure an der rotierenden Goldelektrode beschrieben. Das Reagenz ist elektrochemisch reduzierbar, und dessen Konzentration kann kontrolliert werden, indem eine amperometrische Indikatorelektrode auf einem Potential von -0.25 V gegen ges. Kalomeleleklrode gehalten wird. In 1.0 M Salzsäure können gut ausgebildete Titrationskurven von Proteinen in Gegenwart luftgesättigter Lösungen erhalten werden. Die relative Standardabweichung im Bereich 2–4 g Protein pro 1 Probelösung ist 0.2–0.3%. Das Verfahren ist prinzipiell auf homogene Gemische von Proteinen anwendbar.</abstract><relatedItem type="host"><titleInfo><title>Analytica Chimica Acta</title></titleInfo><titleInfo type="abbreviated"><title>ACA</title></titleInfo><genre type="Journal">journal</genre><originInfo><dateIssued encoding="w3cdtf">197403</dateIssued></originInfo><identifier type="ISSN">0003-2670</identifier><identifier type="PII">S0003-2670(00)X0613-7</identifier><part><date>197403</date><detail type="volume"><number>69</number><caption>vol.</caption></detail><detail type="issue"><number>1</number><caption>no.</caption></detail><extent unit="issue pages"><start>1</start><end>251</end></extent><extent unit="pages"><start>161</start><end>172</end></extent></part></relatedItem><identifier type="istex">52C943B61503F54CCE63D28D68A60A5EB1A6AE20</identifier><identifier type="DOI">10.1016/0003-2670(74)80019-X</identifier><identifier type="PII">0003-2670(74)80019-X</identifier><identifier type="ArticleID">7480019X</identifier><recordInfo><recordContentSource>ELSEVIER</recordContentSource></recordInfo></mods></metadata><enrichments><istex:refBibTEI uri="https://api.istex.fr/document/52C943B61503F54CCE63D28D68A60A5EB1A6AE20/enrichments/refBib"><teiHeader></teiHeader><text><front></front><body></body><back><listBibl><biblStruct><monogr><author><persName><forename type="first">P</forename><forename type="middle">E J</forename><surname>Howe</surname></persName></author><author><persName><surname>Bid</surname></persName></author><imprint><date type="published" when="1921"></date></imprint></monogr></biblStruct><biblStruct><analytic><author><persName><forename type="first">F</forename><surname>Kachmcr 183</surname></persName></author><author><persName><forename type="first">J</forename><surname>Io</surname></persName></author><author><persName><forename type="middle">J</forename><surname>Kuta</surname></persName></author><author><persName><forename type="first">J</forename><surname>Wcbcr</surname></persName></author><author><persName><surname>Koutccky</surname></persName></author></analytic><monogr><title level="m">press. 5 J. H. Kcnncdy. J. Awr. C/ton. Sec.. 82 (1960) 2701. 6 W. Q. Wolfson Couru~~ru.. 25 ( IYGci) 2376. I1 S</title><editor>N. W. Tietz Snundcrs. PhiI:&Iphia. 1970. Chnptcr 5. p. 177. 3 L. Silverman. 8. Schrcincr and D. Glick. J. Cc//. Viol.. 40 761. 4 E. B. Smith and P. W. Car-r. Ard. Chcrn. R. Bctso, M. H. Klappcr and L. B. Anderson, J. Armr. C/rcr~</editor><editor>. Sot</editor><meeting><address>New York</address></meeting><imprint><publisher>McGraw-Hill</publisher><date type="published" when="1955"></date><biblScope unit="page" from="94" to="77"></biblScope></imprint></monogr><note>and. C. Cohn. Artwr. J. C/h. P~~rho/.. I8 ( 1948) 723. 7 R. J. Henry. 81Y7. . I2 C. Tunford. S. Swanson and W. Shore. J. n,ucr. Clrcru. Sot. I3. C. Tnnford. ~tktrrt. Prorci!i Chw.</note></biblStruct></listBibl></back></text></istex:refBibTEI></enrichments></istex></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Informatique/explor/ScrumV1/Data/Istex/Corpus
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000D27 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Istex/Corpus/biblio.hfd -nk 000D27 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Informatique
|area= ScrumV1
|flux= Istex
|étape= Corpus
|type= RBID
|clé= ISTEX:52C943B61503F54CCE63D28D68A60A5EB1A6AE20
|texte= Determination of proteins by amperometric titration with 12-phosphotungstic acid at rotating gold electrodes
}}
| This area was generated with Dilib version V0.6.39. |  |