Modulatory effect of piperine on benzo[ a ]pyrene cytotoxicity and DNA adduct formation in V-79 lung fibroblast cells
Identifieur interne : 000825 ( Istex/Corpus ); précédent : 000824; suivant : 000826Modulatory effect of piperine on benzo[ a ]pyrene cytotoxicity and DNA adduct formation in V-79 lung fibroblast cells
Auteurs : C.-Y. Chu ; J.-P. Chang ; C.-J. WangSource :
- Food and Chemical Toxicology [ 0278-6915 ] ; 1993.
Abstract
Piperine, a major component of black pepper and long peppers, has been reported previously to have an effect on the activation and deactivation of some exogenous substances. In the present study, piperine was found to promote DNA damage and cytotoxicity induced by benzo[a]pyrene (B[a]P) in cultured V-79 lung fibroblast cells. The V-79 cells were treated with a non-toxic dose of piperine (1–20 μm) plus 10 μm B[a]P, or pretreated with piperine for 30 min or 2 hr prior to the administration of 10 μm B[a]P. B[a]P cytotoxicity was potentiated significantly by piperine under each experimental condition. The relative plating efficiency (RPE) was 71% when V-79 cells were exposed to 10 μm B[a]P alone. When the culture was exposed to B[a]P plus piperine or pretreated with piperine for 30 min prior to the administration of B[a]P, the RPE values were 63 and 44% (P < 0.001), respectively. Pretreatment with piperine for 2 hr had no significant effect (P > 0.05). Furthermore, the lowest activities (P < 0.05) of glutathione S-transferase (GST) and uridine diphosphate glucuronyl transferase (UDP-GTase) of piperine-treated V-79 cells occurred 30 min to 1 hr after the piperine pretreatment. Pretreatment of V-79 cells with piperine also caused an increase in the covalent binding of B[a]P-diol-epoxide to DNA, 2.3 times greater than that of the V-79 cells without piperine treatment. These results suggest that the promotion by piperine of B[a]P-induced cytotoxicity in V-79 lung fibroblast cells is due to mechanisms that decrease the activities of GST and UDP-GTase and increase the formation of a B[a]P DNA adduct.
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DOI: 10.1016/0278-6915(94)90076-0
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<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title>Modulatory effect of piperine on benzo[ a ]pyrene cytotoxicity and DNA adduct formation in V-79 lung fibroblast cells</title><author><name sortKey="Chu, C Y" sort="Chu, C Y" uniqKey="Chu C" first="C.-Y." last="Chu">C.-Y. Chu</name><affiliation><mods:affiliation>Institute of Biochemistry, Chung-Shan Medical and Dental College, Taichung, Taiwan, Republic of China</mods:affiliation></affiliation></author><author><name sortKey="Chang, J P" sort="Chang, J P" uniqKey="Chang J" first="J.-P." last="Chang">J.-P. Chang</name><affiliation><mods:affiliation>Institute of Biochemistry, Chung-Shan Medical and Dental College, Taichung, Taiwan, Republic of China</mods:affiliation></affiliation></author><author><name sortKey="Wang, C J" sort="Wang, C J" uniqKey="Wang C" first="C.-J." last="Wang">C.-J. Wang</name><affiliation><mods:affiliation>Institute of Biochemistry, Chung-Shan Medical and Dental College, Taichung, Taiwan, Republic of China</mods:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:F2F03C2F0FB0B83D684718B01F69B08195459AD1</idno><date when="1994" year="1994">1994</date><idno type="doi">10.1016/0278-6915(94)90076-0</idno><idno type="url">https://api.istex.fr/document/F2F03C2F0FB0B83D684718B01F69B08195459AD1/fulltext/pdf</idno><idno type="wicri:Area/Istex/Corpus">000825</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a">Modulatory effect of piperine on benzo[ a ]pyrene cytotoxicity and DNA adduct formation in V-79 lung fibroblast cells</title><author><name sortKey="Chu, C Y" sort="Chu, C Y" uniqKey="Chu C" first="C.-Y." last="Chu">C.-Y. Chu</name><affiliation><mods:affiliation>Institute of Biochemistry, Chung-Shan Medical and Dental College, Taichung, Taiwan, Republic of China</mods:affiliation></affiliation></author><author><name sortKey="Chang, J P" sort="Chang, J P" uniqKey="Chang J" first="J.-P." last="Chang">J.-P. Chang</name><affiliation><mods:affiliation>Institute of Biochemistry, Chung-Shan Medical and Dental College, Taichung, Taiwan, Republic of China</mods:affiliation></affiliation></author><author><name sortKey="Wang, C J" sort="Wang, C J" uniqKey="Wang C" first="C.-J." last="Wang">C.-J. Wang</name><affiliation><mods:affiliation>Institute of Biochemistry, Chung-Shan Medical and Dental College, Taichung, Taiwan, Republic of China</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">Food and Chemical Toxicology</title><title level="j" type="abbrev">FCT</title><idno type="ISSN">0278-6915</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1993">1993</date><biblScope unit="volume">32</biblScope><biblScope unit="issue">4</biblScope><biblScope unit="page" from="373">373</biblScope><biblScope unit="page" to="377">377</biblScope></imprint><idno type="ISSN">0278-6915</idno></series><idno type="istex">F2F03C2F0FB0B83D684718B01F69B08195459AD1</idno><idno type="DOI">10.1016/0278-6915(94)90076-0</idno><idno type="PII">0278-6915(94)90076-0</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0278-6915</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Piperine, a major component of black pepper and long peppers, has been reported previously to have an effect on the activation and deactivation of some exogenous substances. In the present study, piperine was found to promote DNA damage and cytotoxicity induced by benzo[a]pyrene (B[a]P) in cultured V-79 lung fibroblast cells. The V-79 cells were treated with a non-toxic dose of piperine (1–20 μm) plus 10 μm B[a]P, or pretreated with piperine for 30 min or 2 hr prior to the administration of 10 μm B[a]P. B[a]P cytotoxicity was potentiated significantly by piperine under each experimental condition. The relative plating efficiency (RPE) was 71% when V-79 cells were exposed to 10 μm B[a]P alone. When the culture was exposed to B[a]P plus piperine or pretreated with piperine for 30 min prior to the administration of B[a]P, the RPE values were 63 and 44% (P < 0.001), respectively. Pretreatment with piperine for 2 hr had no significant effect (P > 0.05). Furthermore, the lowest activities (P < 0.05) of glutathione S-transferase (GST) and uridine diphosphate glucuronyl transferase (UDP-GTase) of piperine-treated V-79 cells occurred 30 min to 1 hr after the piperine pretreatment. Pretreatment of V-79 cells with piperine also caused an increase in the covalent binding of B[a]P-diol-epoxide to DNA, 2.3 times greater than that of the V-79 cells without piperine treatment. These results suggest that the promotion by piperine of B[a]P-induced cytotoxicity in V-79 lung fibroblast cells is due to mechanisms that decrease the activities of GST and UDP-GTase and increase the formation of a B[a]P DNA adduct.</div></front></TEI><istex><corpusName>elsevier</corpusName><author><json:item><name>C.-Y. Chu</name><affiliations><json:string>Institute of Biochemistry, Chung-Shan Medical and Dental College, Taichung, Taiwan, Republic of China</json:string></affiliations></json:item><json:item><name>J.-P. Chang</name><affiliations><json:string>Institute of Biochemistry, Chung-Shan Medical and Dental College, Taichung, Taiwan, Republic of China</json:string></affiliations></json:item><json:item><name>C.-J. Wang</name><affiliations><json:string>Institute of Biochemistry, Chung-Shan Medical and Dental College, Taichung, Taiwan, Republic of China</json:string></affiliations></json:item></author><subject><json:item><lang><json:string>eng</json:string></lang><value>B[a]P = benzo[a]pyrene</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>BPDE = 7,8-diol-9,10-epoxide-benzopyrene</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>BSA = bovine serum albumin</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>CDNB = 1-chloro-2,4-dinitrobenzene</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>CIP = chloroform/isoamyl alcohol/phenol</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>DMSO = dimethyl sulfoxide</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>GSH = reduced glutathione</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>GST = glutathione S-transferase</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>PAH = polycyclic aromatic hydrocarbon</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>PNP = p-nitrophenol</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>PNPG = p-nitrophenol glucuronate</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>PSN = penicillin-streptomycin mixtures</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>RPE = relative plating efficiency</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>UDPGA = uridine diphosphate glucuronic acid</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>UDP-GTase = uridine diphosphate glucuronyl transferase</value></json:item></subject><language><json:string>eng</json:string></language><abstract>Piperine, a major component of black pepper and long peppers, has been reported previously to have an effect on the activation and deactivation of some exogenous substances. In the present study, piperine was found to promote DNA damage and cytotoxicity induced by benzo[a]pyrene (B[a]P) in cultured V-79 lung fibroblast cells. The V-79 cells were treated with a non-toxic dose of piperine (1–20 μm) plus 10 μm B[a]P, or pretreated with piperine for 30 min or 2 hr prior to the administration of 10 μm B[a]P. B[a]P cytotoxicity was potentiated significantly by piperine under each experimental condition. The relative plating efficiency (RPE) was 71% when V-79 cells were exposed to 10 μm B[a]P alone. When the culture was exposed to B[a]P plus piperine or pretreated with piperine for 30 min prior to the administration of B[a]P, the RPE values were 63 and 44% (P > 0.001), respectively. Pretreatment with piperine for 2 hr had no significant effect (P > 0.05). Furthermore, the lowest activities (P > 0.05) of glutathione S-transferase (GST) and uridine diphosphate glucuronyl transferase (UDP-GTase) of piperine-treated V-79 cells occurred 30 min to 1 hr after the piperine pretreatment. Pretreatment of V-79 cells with piperine also caused an increase in the covalent binding of B[a]P-diol-epoxide to DNA, 2.3 times greater than that of the V-79 cells without piperine treatment. These results suggest that the promotion by piperine of B[a]P-induced cytotoxicity in V-79 lung fibroblast cells is due to mechanisms that decrease the activities of GST and UDP-GTase and increase the formation of a B[a]P DNA adduct.</abstract><qualityIndicators><score>6.885</score><pdfVersion>1.2</pdfVersion><pdfPageSize>533 x 764 pts</pdfPageSize><refBibsNative>true</refBibsNative><keywordCount>15</keywordCount><abstractCharCount>1617</abstractCharCount><pdfWordCount>3885</pdfWordCount><pdfCharCount>23627</pdfCharCount><pdfPageCount>5</pdfPageCount><abstractWordCount>257</abstractWordCount></qualityIndicators><title>Modulatory effect of piperine on benzo[ a ]pyrene cytotoxicity and DNA adduct formation in V-79 lung fibroblast cells</title><pii><json:string>0278-6915(94)90076-0</json:string></pii><genre><json:string>research-article</json:string></genre><serie><pages><last>1019</last><first>1015</first></pages><genre></genre><language><json:string>unknown</json:string></language><title>The measurement of p-nitrophenyl glucuronide</title></serie><host><volume>32</volume><pii><json:string>S0278-6915(00)X0192-5</json:string></pii><pages><last>377</last><first>373</first></pages><issn><json:string>0278-6915</json:string></issn><issue>4</issue><genre><json:string>Journal</json:string></genre><language><json:string>unknown</json:string></language><title>Food and Chemical Toxicology</title><publicationDate>1994</publicationDate></host><categories><wos><json:string>TOXICOLOGY</json:string><json:string>TRANSPLANTATION</json:string><json:string>FOOD SCIENCE & TECHNOLOGY</json:string></wos></categories><publicationDate>1993</publicationDate><copyrightDate>1994</copyrightDate><doi><json:string>10.1016/0278-6915(94)90076-0</json:string></doi><id>F2F03C2F0FB0B83D684718B01F69B08195459AD1</id><fulltext><json:item><original>true</original><mimetype>application/pdf</mimetype><extension>pdf</extension><uri>https://api.istex.fr/document/F2F03C2F0FB0B83D684718B01F69B08195459AD1/fulltext/pdf</uri></json:item><json:item><original>true</original><mimetype>text/plain</mimetype><extension>txt</extension><uri>https://api.istex.fr/document/F2F03C2F0FB0B83D684718B01F69B08195459AD1/fulltext/txt</uri></json:item><json:item><original>false</original><mimetype>application/zip</mimetype><extension>zip</extension><uri>https://api.istex.fr/document/F2F03C2F0FB0B83D684718B01F69B08195459AD1/fulltext/zip</uri></json:item><istex:fulltextTEI uri="https://api.istex.fr/document/F2F03C2F0FB0B83D684718B01F69B08195459AD1/fulltext/tei"><teiHeader><fileDesc><titleStmt><title level="a">Modulatory effect of piperine on benzo[ a ]pyrene cytotoxicity and DNA adduct formation in V-79 lung fibroblast cells</title></titleStmt><publicationStmt><authority>ISTEX</authority><publisher>ELSEVIER</publisher><availability><p>ELSEVIER</p></availability><date>1994</date></publicationStmt><sourceDesc><biblStruct type="inbook"><analytic><title level="a">Modulatory effect of piperine on benzo[ a ]pyrene cytotoxicity and DNA adduct formation in V-79 lung fibroblast cells</title><author><persName><forename type="first">C.-Y.</forename><surname>Chu</surname></persName><affiliation>To whom correspondence should be addressed at: Institute of Biochemistry, Chung-Shan Medical and Dental College, No. 113, Section 2, Ta-Chang Street, Taichung 402, Taiwan, Republic of China.</affiliation><affiliation>Institute of Biochemistry, Chung-Shan Medical and Dental College, Taichung, Taiwan, Republic of China</affiliation></author><author><persName><forename type="first">J.-P.</forename><surname>Chang</surname></persName><affiliation>Institute of Biochemistry, Chung-Shan Medical and Dental College, Taichung, Taiwan, Republic of China</affiliation></author><author><persName><forename type="first">C.-J.</forename><surname>Wang</surname></persName><affiliation>Institute of Biochemistry, Chung-Shan Medical and Dental College, Taichung, Taiwan, Republic of China</affiliation></author></analytic><monogr><title level="j">Food and Chemical Toxicology</title><title level="j" type="abbrev">FCT</title><idno type="pISSN">0278-6915</idno><idno type="PII">S0278-6915(00)X0192-5</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1993"></date><biblScope unit="volume">32</biblScope><biblScope unit="issue">4</biblScope><biblScope unit="page" from="373">373</biblScope><biblScope unit="page" to="377">377</biblScope></imprint></monogr><idno type="istex">F2F03C2F0FB0B83D684718B01F69B08195459AD1</idno><idno type="DOI">10.1016/0278-6915(94)90076-0</idno><idno type="PII">0278-6915(94)90076-0</idno></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>1994</date></creation><langUsage><language ident="en">en</language></langUsage><abstract xml:lang="en"><p>Piperine, a major component of black pepper and long peppers, has been reported previously to have an effect on the activation and deactivation of some exogenous substances. In the present study, piperine was found to promote DNA damage and cytotoxicity induced by benzo[a]pyrene (B[a]P) in cultured V-79 lung fibroblast cells. The V-79 cells were treated with a non-toxic dose of piperine (1–20 μm) plus 10 μm B[a]P, or pretreated with piperine for 30 min or 2 hr prior to the administration of 10 μm B[a]P. B[a]P cytotoxicity was potentiated significantly by piperine under each experimental condition. The relative plating efficiency (RPE) was 71% when V-79 cells were exposed to 10 μm B[a]P alone. When the culture was exposed to B[a]P plus piperine or pretreated with piperine for 30 min prior to the administration of B[a]P, the RPE values were 63 and 44% (P < 0.001), respectively. Pretreatment with piperine for 2 hr had no significant effect (P > 0.05). Furthermore, the lowest activities (P < 0.05) of glutathione S-transferase (GST) and uridine diphosphate glucuronyl transferase (UDP-GTase) of piperine-treated V-79 cells occurred 30 min to 1 hr after the piperine pretreatment. Pretreatment of V-79 cells with piperine also caused an increase in the covalent binding of B[a]P-diol-epoxide to DNA, 2.3 times greater than that of the V-79 cells without piperine treatment. These results suggest that the promotion by piperine of B[a]P-induced cytotoxicity in V-79 lung fibroblast cells is due to mechanisms that decrease the activities of GST and UDP-GTase and increase the formation of a B[a]P DNA adduct.</p></abstract><textClass><keywords scheme="keyword"><list><head>Abbreviations</head><item><term>B[a]P = benzo[a]pyrene</term></item><item><term>BPDE = 7,8-diol-9,10-epoxide-benzopyrene</term></item><item><term>BSA = bovine serum albumin</term></item><item><term>CDNB = 1-chloro-2,4-dinitrobenzene</term></item><item><term>CIP = chloroform/isoamyl alcohol/phenol</term></item><item><term>DMSO = dimethyl sulfoxide</term></item><item><term>GSH = reduced glutathione</term></item><item><term>GST = glutathione S-transferase</term></item><item><term>PAH = polycyclic aromatic hydrocarbon</term></item><item><term>PNP = p-nitrophenol</term></item><item><term>PNPG = p-nitrophenol glucuronate</term></item><item><term>PSN = penicillin-streptomycin mixtures</term></item><item><term>RPE = relative plating efficiency</term></item><item><term>UDPGA = uridine diphosphate glucuronic acid</term></item><item><term>UDP-GTase = uridine diphosphate glucuronyl transferase</term></item></list></keywords></textClass></profileDesc><revisionDesc><change when="1993-09-14">Registration</change><change when="1993">Published</change></revisionDesc></teiHeader></istex:fulltextTEI></fulltext><metadata><istex:metadataXml wicri:clean="Elsevier, elements deleted: tail"><istex:xmlDeclaration>version="1.0" encoding="utf-8"</istex:xmlDeclaration><istex:docType PUBLIC="-//ES//DTD journal article DTD version 4.5.2//EN//XML" URI="art452.dtd" name="istex:docType"></istex:docType><istex:document><converted-article version="4.5.2" docsubtype="fla"><item-info><jid>FCT</jid><aid>94900760</aid><ce:pii>0278-6915(94)90076-0</ce:pii><ce:doi>10.1016/0278-6915(94)90076-0</ce:doi><ce:copyright type="unknown" year="1994"></ce:copyright></item-info><head><ce:title>Modulatory effect of piperine on benzo[<ce:italic>a</ce:italic>]pyrene cytotoxicity and DNA adduct formation in V-79 lung fibroblast cells</ce:title><ce:author-group><ce:author><ce:given-name>C.-Y.</ce:given-name><ce:surname>Chu</ce:surname><ce:cross-ref refid="COR1"><ce:sup>∗</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>J.-P.</ce:given-name><ce:surname>Chang</ce:surname></ce:author><ce:author><ce:given-name>C.-J.</ce:given-name><ce:surname>Wang</ce:surname></ce:author><ce:affiliation><ce:textfn>Institute of Biochemistry, Chung-Shan Medical and Dental College, Taichung, Taiwan, Republic of China</ce:textfn></ce:affiliation><ce:correspondence id="COR1"><ce:label>∗</ce:label><ce:text>To whom correspondence should be addressed at: Institute of Biochemistry, Chung-Shan Medical and Dental College, No. 113, Section 2, Ta-Chang Street, Taichung 402, Taiwan, Republic of China.</ce:text></ce:correspondence></ce:author-group><ce:date-accepted day="14" month="9" year="1993"></ce:date-accepted><ce:abstract><ce:section-title>Abstract</ce:section-title><ce:abstract-sec><ce:simple-para>Piperine, a major component of black pepper and long peppers, has been reported previously to have an effect on the activation and deactivation of some exogenous substances. In the present study, piperine was found to promote DNA damage and cytotoxicity induced by benzo[<ce:italic>a</ce:italic>]pyrene (B[<ce:italic>a</ce:italic>]P) in cultured V-79 lung fibroblast cells. The V-79 cells were treated with a non-toxic dose of piperine (1–20 μ<ce:small-caps>m</ce:small-caps>) plus 10 μ<ce:small-caps>m</ce:small-caps> B[<ce:italic>a</ce:italic>]P, or pretreated with piperine for 30 min or 2 hr prior to the administration of 10 μ<ce:small-caps>m</ce:small-caps> B[<ce:italic>a</ce:italic>]P. B[<ce:italic>a</ce:italic>]P cytotoxicity was potentiated significantly by piperine under each experimental condition. The relative plating efficiency (RPE) was 71% when V-79 cells were exposed to 10 μ<ce:small-caps>m</ce:small-caps> B[<ce:italic>a</ce:italic>]P alone. When the culture was exposed to B[<ce:italic>a</ce:italic>]P plus piperine or pretreated with piperine for 30 min prior to the administration of B[<ce:italic>a</ce:italic>]P, the RPE values were 63 and 44% (<ce:italic>P</ce:italic> < 0.001), respectively. Pretreatment with piperine for 2 hr had no significant effect (<ce:italic>P</ce:italic> > 0.05). Furthermore, the lowest activities (<ce:italic>P</ce:italic> < 0.05) of glutathione <ce:italic>S</ce:italic>-transferase (GST) and uridine diphosphate glucuronyl transferase (UDP-GTase) of piperine-treated V-79 cells occurred 30 min to 1 hr after the piperine pretreatment. Pretreatment of V-79 cells with piperine also caused an increase in the covalent binding of B[<ce:italic>a</ce:italic>]P-diol-epoxide to DNA, 2.3 times greater than that of the V-79 cells without piperine treatment. These results suggest that the promotion by piperine of B[<ce:italic>a</ce:italic>]P-induced cytotoxicity in V-79 lung fibroblast cells is due to mechanisms that decrease the activities of GST and UDP-GTase and increase the formation of a B[<ce:italic>a</ce:italic>]P DNA adduct.</ce:simple-para></ce:abstract-sec></ce:abstract><ce:keywords class="abr"><ce:section-title>Abbreviations</ce:section-title><ce:keyword><ce:text>B[<ce:italic>a</ce:italic>]P = benzo[<ce:italic>a</ce:italic>]pyrene</ce:text></ce:keyword><ce:keyword><ce:text>BPDE = 7,8-diol-9,10-epoxide-benzopyrene</ce:text></ce:keyword><ce:keyword><ce:text>BSA = bovine serum albumin</ce:text></ce:keyword><ce:keyword><ce:text>CDNB = 1-chloro-2,4-dinitrobenzene</ce:text></ce:keyword><ce:keyword><ce:text>CIP = chloroform/isoamyl alcohol/phenol</ce:text></ce:keyword><ce:keyword><ce:text>DMSO = dimethyl sulfoxide</ce:text></ce:keyword><ce:keyword><ce:text>GSH = reduced glutathione</ce:text></ce:keyword><ce:keyword><ce:text>GST = glutathione <ce:italic>S</ce:italic>-transferase</ce:text></ce:keyword><ce:keyword><ce:text>PAH = polycyclic aromatic hydrocarbon</ce:text></ce:keyword><ce:keyword><ce:text>PNP = <ce:italic>p</ce:italic>-nitrophenol</ce:text></ce:keyword><ce:keyword><ce:text>PNPG = <ce:italic>p</ce:italic>-nitrophenol glucuronate</ce:text></ce:keyword><ce:keyword><ce:text>PSN = penicillin-streptomycin mixtures</ce:text></ce:keyword><ce:keyword><ce:text>RPE = relative plating efficiency</ce:text></ce:keyword><ce:keyword><ce:text>UDPGA = uridine diphosphate glucuronic acid</ce:text></ce:keyword><ce:keyword><ce:text>UDP-GTase = uridine diphosphate glucuronyl transferase</ce:text></ce:keyword></ce:keywords></head></converted-article></istex:document></istex:metadataXml><mods version="3.6"><titleInfo><title>Modulatory effect of piperine on benzo[ a ]pyrene cytotoxicity and DNA adduct formation in V-79 lung fibroblast cells</title></titleInfo><titleInfo type="alternative" contentType="CDATA"><title>Modulatory effect of piperine on benzo[</title></titleInfo><name type="personal"><namePart type="given">C.-Y.</namePart><namePart type="family">Chu</namePart><affiliation>Institute of Biochemistry, Chung-Shan Medical and Dental College, Taichung, Taiwan, Republic of China</affiliation><description>To whom correspondence should be addressed at: Institute of Biochemistry, Chung-Shan Medical and Dental College, No. 113, Section 2, Ta-Chang Street, Taichung 402, Taiwan, Republic of China.</description><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">J.-P.</namePart><namePart type="family">Chang</namePart><affiliation>Institute of Biochemistry, Chung-Shan Medical and Dental College, Taichung, Taiwan, Republic of China</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">C.-J.</namePart><namePart type="family">Wang</namePart><affiliation>Institute of Biochemistry, Chung-Shan Medical and Dental College, Taichung, Taiwan, Republic of China</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="Full-length article"></genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">1993</dateIssued><dateValid encoding="w3cdtf">1993-09-14</dateValid><copyrightDate encoding="w3cdtf">1994</copyrightDate></originInfo><language><languageTerm type="code" authority="iso639-2b">eng</languageTerm><languageTerm type="code" authority="rfc3066">en</languageTerm></language><physicalDescription><internetMediaType>text/html</internetMediaType></physicalDescription><abstract lang="en">Piperine, a major component of black pepper and long peppers, has been reported previously to have an effect on the activation and deactivation of some exogenous substances. In the present study, piperine was found to promote DNA damage and cytotoxicity induced by benzo[a]pyrene (B[a]P) in cultured V-79 lung fibroblast cells. The V-79 cells were treated with a non-toxic dose of piperine (1–20 μm) plus 10 μm B[a]P, or pretreated with piperine for 30 min or 2 hr prior to the administration of 10 μm B[a]P. B[a]P cytotoxicity was potentiated significantly by piperine under each experimental condition. The relative plating efficiency (RPE) was 71% when V-79 cells were exposed to 10 μm B[a]P alone. When the culture was exposed to B[a]P plus piperine or pretreated with piperine for 30 min prior to the administration of B[a]P, the RPE values were 63 and 44% (P < 0.001), respectively. Pretreatment with piperine for 2 hr had no significant effect (P > 0.05). Furthermore, the lowest activities (P < 0.05) of glutathione S-transferase (GST) and uridine diphosphate glucuronyl transferase (UDP-GTase) of piperine-treated V-79 cells occurred 30 min to 1 hr after the piperine pretreatment. Pretreatment of V-79 cells with piperine also caused an increase in the covalent binding of B[a]P-diol-epoxide to DNA, 2.3 times greater than that of the V-79 cells without piperine treatment. These results suggest that the promotion by piperine of B[a]P-induced cytotoxicity in V-79 lung fibroblast cells is due to mechanisms that decrease the activities of GST and UDP-GTase and increase the formation of a B[a]P DNA adduct.</abstract><subject><genre>Abbreviations</genre><topic>B[a]P = benzo[a]pyrene</topic><topic>BPDE = 7,8-diol-9,10-epoxide-benzopyrene</topic><topic>BSA = bovine serum albumin</topic><topic>CDNB = 1-chloro-2,4-dinitrobenzene</topic><topic>CIP = chloroform/isoamyl alcohol/phenol</topic><topic>DMSO = dimethyl sulfoxide</topic><topic>GSH = reduced glutathione</topic><topic>GST = glutathione S-transferase</topic><topic>PAH = polycyclic aromatic hydrocarbon</topic><topic>PNP = p-nitrophenol</topic><topic>PNPG = p-nitrophenol glucuronate</topic><topic>PSN = penicillin-streptomycin mixtures</topic><topic>RPE = relative plating efficiency</topic><topic>UDPGA = uridine diphosphate glucuronic acid</topic><topic>UDP-GTase = uridine diphosphate glucuronyl transferase</topic></subject><relatedItem type="host"><titleInfo><title>Food and Chemical Toxicology</title></titleInfo><titleInfo type="abbreviated"><title>FCT</title></titleInfo><genre type="Journal">journal</genre><originInfo><dateIssued encoding="w3cdtf">199404</dateIssued></originInfo><identifier type="ISSN">0278-6915</identifier><identifier type="PII">S0278-6915(00)X0192-5</identifier><part><date>199404</date><detail type="volume"><number>32</number><caption>vol.</caption></detail><detail type="issue"><number>4</number><caption>no.</caption></detail><extent unit="issue pages"><start>297</start><end>395</end></extent><extent unit="pages"><start>373</start><end>377</end></extent></part></relatedItem><identifier type="istex">F2F03C2F0FB0B83D684718B01F69B08195459AD1</identifier><identifier type="DOI">10.1016/0278-6915(94)90076-0</identifier><identifier type="PII">0278-6915(94)90076-0</identifier><recordInfo><recordContentSource>ELSEVIER</recordContentSource></recordInfo></mods></metadata><enrichments><istex:catWosTEI uri="https://api.istex.fr/document/F2F03C2F0FB0B83D684718B01F69B08195459AD1/enrichments/catWos"><teiHeader><profileDesc><textClass><classCode scheme="WOS">TOXICOLOGY</classCode><classCode scheme="WOS">TRANSPLANTATION</classCode><classCode scheme="WOS">FOOD SCIENCE & TECHNOLOGY</classCode></textClass></profileDesc></teiHeader></istex:catWosTEI></enrichments></istex></record>Pour manipuler ce document sous Unix (Dilib)
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