CR07*IMMUNOHISTOCHEMISTRY FOR LOSS OF EXPRESSION OF MISMATCH REPAIR GENE PROTEINS IN YOUNG PATIENTS WITH COLORECTAL CANCER: THE AUCKLAND EXPERIENCE
Identifieur interne : 001174 ( Istex/Corpus ); précédent : 001173; suivant : 001175CR07*IMMUNOHISTOCHEMISTRY FOR LOSS OF EXPRESSION OF MISMATCH REPAIR GENE PROTEINS IN YOUNG PATIENTS WITH COLORECTAL CANCER: THE AUCKLAND EXPERIENCE
Auteurs : D. M. Wright ; S. Parry ; J. Arnold ; I. Bissett ; M. Hulme Oir ; B. ParrySource :
- ANZ Journal of Surgery [ 1445-1433 ] ; 2009-05.
Abstract
Recently, in Australasia, a plea to screen all young patients with colorectal cancer (CRC) for Lynch syndrome has been advanced1. Since 2001 the three public hospitals in Auckland have had the policy of undertaking immunohistochemical (IHC) testing on the tumours of patients aged 50 years and under to detect loss of expression (LOE) of protein products for the mismatch repair (MMR) genes MLH1, MSH2, MSH6 and PMS2. Purpose: To determine (1) the completeness of patient capture, (2) the incidence of LOE by IHC of the 4 gene proteins, and (3) to correlate IHC results with those of subsequent genetic testing for the corresponding germline mutations. Methodology: Retrospective review of the prospectively gathered clinical, pathological, and genetic records of all patients diagnosed with CRC aged 50 years and below between January 2001 and December 2007. Results: 244 patients aged 50 years or below with CRC were diagnosed in this period. 212 (86.9%) patients’ tumours underwent IHC, 147 of which had all 4 gene products studied. 30 (14.2%) tumours had LOE for one or more MMR proteins. 25/30 patients were offered genetic testing of whom 3 declined. So far 8 out of 16 patients tested have a confirmed germline mutation. Only 3/8 patients report a family history of CRC. Conclusion: Tumour IHC reveals LOE for one of the four MMR gene proteins in approximately 14% of young patients developing CRC and in half subsequent genetic testing will identify Lynch syndrome. A policy of mandatory testing is confirmed but audit of performance and appropriate referral is required.
Url:
DOI: 10.1111/j.1445-2197.2009.04915_7.x
Links to Exploration step
ISTEX:C61AF7AD23BF14689F1B4649130DCC991B2F94A3Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">CR07*IMMUNOHISTOCHEMISTRY FOR LOSS OF EXPRESSION OF MISMATCH REPAIR GENE PROTEINS IN YOUNG PATIENTS WITH COLORECTAL CANCER: THE AUCKLAND EXPERIENCE</title><author><name sortKey="Wright, D M" sort="Wright, D M" uniqKey="Wright D" first="D. M." last="Wright">D. M. Wright</name><affiliation><mods:affiliation>Auckland City Hospital, Auckland, New Zealand</mods:affiliation></affiliation></author><author><name sortKey="Parry, S" sort="Parry, S" uniqKey="Parry S" first="S." last="Parry">S. Parry</name><affiliation><mods:affiliation>Auckland City Hospital, Auckland, New Zealand</mods:affiliation></affiliation></author><author><name sortKey="Arnold, J" sort="Arnold, J" uniqKey="Arnold J" first="J." last="Arnold">J. Arnold</name><affiliation><mods:affiliation>Auckland City Hospital, Auckland, New Zealand</mods:affiliation></affiliation></author><author><name sortKey="Bissett, I" sort="Bissett, I" uniqKey="Bissett I" first="I." last="Bissett">I. Bissett</name><affiliation><mods:affiliation>Auckland City Hospital, Auckland, New Zealand</mods:affiliation></affiliation></author><author><name sortKey="Hulme Oir, M" sort="Hulme Oir, M" uniqKey="Hulme Oir M" first="M." last="Hulme Oir">M. Hulme Oir</name><affiliation><mods:affiliation>Auckland City Hospital, Auckland, New Zealand</mods:affiliation></affiliation></author><author><name sortKey="Parry, B" sort="Parry, B" uniqKey="Parry B" first="B." last="Parry">B. Parry</name><affiliation><mods:affiliation>Auckland City Hospital, Auckland, New Zealand</mods:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:C61AF7AD23BF14689F1B4649130DCC991B2F94A3</idno><date when="2009" year="2009">2009</date><idno type="doi">10.1111/j.1445-2197.2009.04915_7.x</idno><idno type="url">https://api.istex.fr/document/C61AF7AD23BF14689F1B4649130DCC991B2F94A3/fulltext/pdf</idno><idno type="wicri:Area/Istex/Corpus">001174</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">CR07*IMMUNOHISTOCHEMISTRY FOR LOSS OF EXPRESSION OF MISMATCH REPAIR GENE PROTEINS IN YOUNG PATIENTS WITH COLORECTAL CANCER: THE AUCKLAND EXPERIENCE</title><author><name sortKey="Wright, D M" sort="Wright, D M" uniqKey="Wright D" first="D. M." last="Wright">D. M. Wright</name><affiliation><mods:affiliation>Auckland City Hospital, Auckland, New Zealand</mods:affiliation></affiliation></author><author><name sortKey="Parry, S" sort="Parry, S" uniqKey="Parry S" first="S." last="Parry">S. Parry</name><affiliation><mods:affiliation>Auckland City Hospital, Auckland, New Zealand</mods:affiliation></affiliation></author><author><name sortKey="Arnold, J" sort="Arnold, J" uniqKey="Arnold J" first="J." last="Arnold">J. Arnold</name><affiliation><mods:affiliation>Auckland City Hospital, Auckland, New Zealand</mods:affiliation></affiliation></author><author><name sortKey="Bissett, I" sort="Bissett, I" uniqKey="Bissett I" first="I." last="Bissett">I. Bissett</name><affiliation><mods:affiliation>Auckland City Hospital, Auckland, New Zealand</mods:affiliation></affiliation></author><author><name sortKey="Hulme Oir, M" sort="Hulme Oir, M" uniqKey="Hulme Oir M" first="M." last="Hulme Oir">M. Hulme Oir</name><affiliation><mods:affiliation>Auckland City Hospital, Auckland, New Zealand</mods:affiliation></affiliation></author><author><name sortKey="Parry, B" sort="Parry, B" uniqKey="Parry B" first="B." last="Parry">B. Parry</name><affiliation><mods:affiliation>Auckland City Hospital, Auckland, New Zealand</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">ANZ Journal of Surgery</title><idno type="ISSN">1445-1433</idno><idno type="eISSN">1445-2197</idno><imprint><publisher>Blackwell Publishing Asia</publisher><pubPlace>Melbourne, Australia</pubPlace><date type="published" when="2009-05">2009-05</date><biblScope unit="volume">79</biblScope><biblScope unit="supplement">s1</biblScope><biblScope unit="page" from="A10">A10</biblScope><biblScope unit="page" to="A10">A10</biblScope></imprint><idno type="ISSN">1445-1433</idno></series><idno type="istex">C61AF7AD23BF14689F1B4649130DCC991B2F94A3</idno><idno type="DOI">10.1111/j.1445-2197.2009.04915_7.x</idno><idno type="ArticleID">ANS4915_7</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">1445-1433</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Recently, in Australasia, a plea to screen all young patients with colorectal cancer (CRC) for Lynch syndrome has been advanced1. Since 2001 the three public hospitals in Auckland have had the policy of undertaking immunohistochemical (IHC) testing on the tumours of patients aged 50 years and under to detect loss of expression (LOE) of protein products for the mismatch repair (MMR) genes MLH1, MSH2, MSH6 and PMS2. Purpose: To determine (1) the completeness of patient capture, (2) the incidence of LOE by IHC of the 4 gene proteins, and (3) to correlate IHC results with those of subsequent genetic testing for the corresponding germline mutations. Methodology: Retrospective review of the prospectively gathered clinical, pathological, and genetic records of all patients diagnosed with CRC aged 50 years and below between January 2001 and December 2007. Results: 244 patients aged 50 years or below with CRC were diagnosed in this period. 212 (86.9%) patients’ tumours underwent IHC, 147 of which had all 4 gene products studied. 30 (14.2%) tumours had LOE for one or more MMR proteins. 25/30 patients were offered genetic testing of whom 3 declined. So far 8 out of 16 patients tested have a confirmed germline mutation. Only 3/8 patients report a family history of CRC. Conclusion: Tumour IHC reveals LOE for one of the four MMR gene proteins in approximately 14% of young patients developing CRC and in half subsequent genetic testing will identify Lynch syndrome. A policy of mandatory testing is confirmed but audit of performance and appropriate referral is required.</div></front></TEI><istex><corpusName>wiley</corpusName><author><json:item><name>D. M. Wright</name><affiliations><json:string>Auckland City Hospital, Auckland, New Zealand</json:string></affiliations></json:item><json:item><name>S. Parry</name><affiliations><json:string>Auckland City Hospital, Auckland, New Zealand</json:string></affiliations></json:item><json:item><name>J. Arnold</name><affiliations><json:string>Auckland City Hospital, Auckland, New Zealand</json:string></affiliations></json:item><json:item><name>I. Bissett</name><affiliations><json:string>Auckland City Hospital, Auckland, New Zealand</json:string></affiliations></json:item><json:item><name>M. Hulme‐Moir</name><affiliations><json:string>Auckland City Hospital, Auckland, New Zealand</json:string></affiliations></json:item><json:item><name>B. Parry</name><affiliations><json:string>Auckland City Hospital, Auckland, New Zealand</json:string></affiliations></json:item></author><articleId><json:string>ANS4915_7</json:string></articleId><language><json:string>eng</json:string></language><abstract>Recently, in Australasia, a plea to screen all young patients with colorectal cancer (CRC) for Lynch syndrome has been advanced1. Since 2001 the three public hospitals in Auckland have had the policy of undertaking immunohistochemical (IHC) testing on the tumours of patients aged 50 years and under to detect loss of expression (LOE) of protein products for the mismatch repair (MMR) genes MLH1, MSH2, MSH6 and PMS2. Purpose: To determine (1) the completeness of patient capture, (2) the incidence of LOE by IHC of the 4 gene proteins, and (3) to correlate IHC results with those of subsequent genetic testing for the corresponding germline mutations. Methodology: Retrospective review of the prospectively gathered clinical, pathological, and genetic records of all patients diagnosed with CRC aged 50 years and below between January 2001 and December 2007. Results: 244 patients aged 50 years or below with CRC were diagnosed in this period. 212 (86.9%) patients’ tumours underwent IHC, 147 of which had all 4 gene products studied. 30 (14.2%) tumours had LOE for one or more MMR proteins. 25/30 patients were offered genetic testing of whom 3 declined. So far 8 out of 16 patients tested have a confirmed germline mutation. Only 3/8 patients report a family history of CRC. Conclusion: Tumour IHC reveals LOE for one of the four MMR gene proteins in approximately 14% of young patients developing CRC and in half subsequent genetic testing will identify Lynch syndrome. A policy of mandatory testing is confirmed but audit of performance and appropriate referral is required.</abstract><qualityIndicators><score>8.5</score><pdfVersion>1.4</pdfVersion><pdfPageSize>595.276 x 779.528 pts</pdfPageSize><refBibsNative>true</refBibsNative><keywordCount>0</keywordCount><abstractCharCount>1587</abstractCharCount><pdfWordCount>8993</pdfWordCount><pdfCharCount>55889</pdfCharCount><pdfPageCount>9</pdfPageCount><abstractWordCount>251</abstractWordCount></qualityIndicators><title>CR07*IMMUNOHISTOCHEMISTRY FOR LOSS OF EXPRESSION OF MISMATCH REPAIR GENE PROTEINS IN YOUNG PATIENTS WITH COLORECTAL CANCER: THE AUCKLAND EXPERIENCE</title><genre.original><json:string>abstract</json:string></genre.original><genre><json:string>abstract</json:string></genre><host><volume>79</volume><publisherId><json:string>ANS</json:string></publisherId><pages><total>1</total><last>A10</last><first>A10</first></pages><issn><json:string>1445-1433</json:string></issn><genre><json:string>journal</json:string></genre><language><json:string>unknown</json:string></language><eissn><json:string>1445-2197</json:string></eissn><title>ANZ Journal of Surgery</title><doi><json:string>10.1111/(ISSN)1445-2197</json:string></doi></host><publicationDate>2009</publicationDate><copyrightDate>2009</copyrightDate><doi><json:string>10.1111/j.1445-2197.2009.04915_7.x</json:string></doi><id>C61AF7AD23BF14689F1B4649130DCC991B2F94A3</id><fulltext><json:item><original>true</original><mimetype>application/pdf</mimetype><extension>pdf</extension><uri>https://api.istex.fr/document/C61AF7AD23BF14689F1B4649130DCC991B2F94A3/fulltext/pdf</uri></json:item><json:item><original>false</original><mimetype>application/zip</mimetype><extension>zip</extension><uri>https://api.istex.fr/document/C61AF7AD23BF14689F1B4649130DCC991B2F94A3/fulltext/zip</uri></json:item><istex:fulltextTEI uri="https://api.istex.fr/document/C61AF7AD23BF14689F1B4649130DCC991B2F94A3/fulltext/tei"><teiHeader><fileDesc><titleStmt><title level="a" type="main" xml:lang="en">CR07*IMMUNOHISTOCHEMISTRY FOR LOSS OF EXPRESSION OF MISMATCH REPAIR GENE PROTEINS IN YOUNG PATIENTS WITH COLORECTAL CANCER: THE AUCKLAND EXPERIENCE</title></titleStmt><publicationStmt><authority>ISTEX</authority><publisher>Blackwell Publishing Asia</publisher><pubPlace>Melbourne, Australia</pubPlace><availability><p>WILEY</p></availability><date>2009</date></publicationStmt><sourceDesc><biblStruct type="inbook"><analytic><title level="a" type="main" xml:lang="en">CR07*IMMUNOHISTOCHEMISTRY FOR LOSS OF EXPRESSION OF MISMATCH REPAIR GENE PROTEINS IN YOUNG PATIENTS WITH COLORECTAL CANCER: THE AUCKLAND EXPERIENCE</title><author><persName><forename type="first">D. M.</forename><surname>Wright</surname></persName><affiliation>Auckland City Hospital, Auckland, New Zealand</affiliation></author><author><persName><forename type="first">S.</forename><surname>Parry</surname></persName><affiliation>Auckland City Hospital, Auckland, New Zealand</affiliation></author><author><persName><forename type="first">J.</forename><surname>Arnold</surname></persName><affiliation>Auckland City Hospital, Auckland, New Zealand</affiliation></author><author><persName><forename type="first">I.</forename><surname>Bissett</surname></persName><affiliation>Auckland City Hospital, Auckland, New Zealand</affiliation></author><author><persName><forename type="first">M.</forename><surname>Hulme‐Moir</surname></persName><affiliation>Auckland City Hospital, Auckland, New Zealand</affiliation></author><author><persName><forename type="first">B.</forename><surname>Parry</surname></persName><affiliation>Auckland City Hospital, Auckland, New Zealand</affiliation></author></analytic><monogr><title level="j">ANZ Journal of Surgery</title><idno type="pISSN">1445-1433</idno><idno type="eISSN">1445-2197</idno><idno type="DOI">10.1111/(ISSN)1445-2197</idno><imprint><publisher>Blackwell Publishing Asia</publisher><pubPlace>Melbourne, Australia</pubPlace><date type="published" when="2009-05"></date><biblScope unit="volume">79</biblScope><biblScope unit="supplement">s1</biblScope><biblScope unit="page" from="A10">A10</biblScope><biblScope unit="page" to="A10">A10</biblScope></imprint></monogr><idno type="istex">C61AF7AD23BF14689F1B4649130DCC991B2F94A3</idno><idno type="DOI">10.1111/j.1445-2197.2009.04915_7.x</idno><idno type="ArticleID">ANS4915_7</idno></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>2009</date></creation><langUsage><language ident="en">en</language></langUsage><abstract xml:lang="en"><p>Recently, in Australasia, a plea to screen all young patients with colorectal cancer (CRC) for Lynch syndrome has been advanced1. Since 2001 the three public hospitals in Auckland have had the policy of undertaking immunohistochemical (IHC) testing on the tumours of patients aged 50 years and under to detect loss of expression (LOE) of protein products for the mismatch repair (MMR) genes MLH1, MSH2, MSH6 and PMS2. Purpose: To determine (1) the completeness of patient capture, (2) the incidence of LOE by IHC of the 4 gene proteins, and (3) to correlate IHC results with those of subsequent genetic testing for the corresponding germline mutations. Methodology: Retrospective review of the prospectively gathered clinical, pathological, and genetic records of all patients diagnosed with CRC aged 50 years and below between January 2001 and December 2007. Results: 244 patients aged 50 years or below with CRC were diagnosed in this period. 212 (86.9%) patients’ tumours underwent IHC, 147 of which had all 4 gene products studied. 30 (14.2%) tumours had LOE for one or more MMR proteins. 25/30 patients were offered genetic testing of whom 3 declined. So far 8 out of 16 patients tested have a confirmed germline mutation. Only 3/8 patients report a family history of CRC. Conclusion: Tumour IHC reveals LOE for one of the four MMR gene proteins in approximately 14% of young patients developing CRC and in half subsequent genetic testing will identify Lynch syndrome. A policy of mandatory testing is confirmed but audit of performance and appropriate referral is required.</p></abstract></profileDesc><revisionDesc><change when="2009-05">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><original>false</original><mimetype>text/plain</mimetype><extension>txt</extension><uri>https://api.istex.fr/document/C61AF7AD23BF14689F1B4649130DCC991B2F94A3/fulltext/txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Wiley, elements deleted: body"><istex:xmlDeclaration>version="1.0" encoding="UTF-8" standalone="yes"</istex:xmlDeclaration><istex:document><component version="2.0" type="serialArticle" xml:lang="en"><header><publicationMeta level="product"><publisherInfo><publisherName>Blackwell Publishing Asia</publisherName><publisherLoc>Melbourne, Australia</publisherLoc></publisherInfo><doi origin="wiley" registered="yes">10.1111/(ISSN)1445-2197</doi><issn type="print">1445-1433</issn><issn type="electronic">1445-2197</issn><idGroup><id type="product" value="ANS"></id><id type="publisherDivision" value="ST"></id></idGroup><titleGroup><title type="main" sort="ANZ JOURNAL SURGERY">ANZ Journal of Surgery</title></titleGroup></publicationMeta><publicationMeta level="part" position="05000"><doi origin="wiley">10.1111/ans.2009.79.issue-s1</doi><titleGroup><title type="specialIssueTitle">Abstracts of the Royal Australasian College of Surgeons Annual Scientific Congress, 6–9 May 2009, Brisbane, Queensland, Australia</title></titleGroup><numberingGroup><numbering type="journalVolume" number="79">79</numbering><numbering type="supplement">s1</numbering></numberingGroup><coverDate startDate="2009-05">May 2009</coverDate></publicationMeta><publicationMeta level="unit" type="abstract" position="038" status="forIssue"><doi origin="wiley">10.1111/j.1445-2197.2009.04915_7.x</doi><idGroup><id type="unit" value="ANS4915_7"></id></idGroup><countGroup><count type="pageTotal" number="1"></count></countGroup><titleGroup><title type="tocHeading1">Royal Australasian College of Surgeons Annual Scientific Congress, 6–9 May 2009, Brisbane, Queensland, Australia. Conference Abstracts
Colorectal Surgery Program Abstracts</title></titleGroup><copyright>Journal compilation © 2009 The Royal Australasian College of Surgeons</copyright><eventGroup><event type="firstOnline" date="2009-04-27"></event><event type="publishedOnlineFinalForm" date="2009-04-27"></event><event type="xmlConverted" agent="Converter:BPG_TO_WML3G version:2.3.2 mode:FullText source:HeaderRef result:HeaderRef" date="2010-03-13"></event><event type="xmlConverted" agent="Converter:WILEY_ML3G_TO_WILEY_ML3GV2 version:3.8.8" date="2014-01-04"></event><event type="xmlConverted" agent="Converter:WML3G_To_WML3G version:4.1.7 mode:FullText,remove_FC" date="2014-10-14"></event></eventGroup><numberingGroup><numbering type="pageFirst">A10</numbering><numbering type="pageLast">A10</numbering></numberingGroup><linkGroup><link type="toTypesetVersion" href="file:ANS.ANS4915_7.pdf"></link></linkGroup></publicationMeta><contentMeta><countGroup><count type="figureTotal" number="0"></count><count type="tableTotal" number="0"></count><count type="formulaTotal" number="0"></count><count type="referenceTotal" number="0"></count><count type="wordTotal" number="5149"></count><count type="linksPubMed" number="0"></count><count type="linksCrossRef" number="0"></count></countGroup><titleGroup><title type="main">CR07
*IMMUNOHISTOCHEMISTRY FOR LOSS OF EXPRESSION OF MISMATCH REPAIR GENE PROTEINS IN YOUNG PATIENTS WITH COLORECTAL CANCER: THE AUCKLAND EXPERIENCE</title><title type="shortAuthors"><i>RACS Annual Scientific Congress</i>, 2009</title><title type="short"><i>ANZ J. Surg.</i> 2009; 79 (Suppl. 1)</title></titleGroup><creators><creator creatorRole="author" xml:id="cr1" affiliationRef="#aff-1-1"><personName><givenNames>D. M.</givenNames><familyName>Wright</familyName></personName></creator><creator creatorRole="author" xml:id="cr2" affiliationRef="#aff-1-1"><personName><givenNames>S.</givenNames><familyName>Parry</familyName></personName></creator><creator creatorRole="author" xml:id="cr3" affiliationRef="#aff-1-1"><personName><givenNames>J.</givenNames><familyName>Arnold</familyName></personName></creator><creator creatorRole="author" xml:id="cr4" affiliationRef="#aff-1-1"><personName><givenNames>I.</givenNames><familyName>Bissett</familyName></personName></creator><creator creatorRole="author" xml:id="cr5" affiliationRef="#aff-1-1"><personName><givenNames>M.</givenNames><familyName>Hulme‐Moir</familyName></personName></creator><creator creatorRole="author" xml:id="cr6" affiliationRef="#aff-1-1"><personName><givenNames>B.</givenNames><familyName>Parry</familyName></personName></creator></creators><affiliationGroup><affiliation xml:id="aff-1-1" countryCode="NZ"><unparsedAffiliation>Auckland City Hospital, Auckland, New Zealand</unparsedAffiliation></affiliation></affiliationGroup><abstractGroup><abstract type="main" xml:lang="en"><p>Recently, in Australasia, a plea to screen all young patients with colorectal cancer (CRC) for Lynch syndrome has been advanced<link href="#b1"><sup>1</sup></link>.</p><p>Since 2001 the three public hospitals in Auckland have had the policy of undertaking immunohistochemical (IHC) testing on the tumours of patients aged 50 years and under to detect loss of expression (LOE) of protein products for the mismatch repair (MMR) genes MLH1, MSH2, MSH6 and PMS2.</p><p><b>Purpose: </b> To determine (1) the completeness of patient capture, (2) the incidence of LOE by IHC of the 4 gene proteins, and (3) to correlate IHC results with those of subsequent genetic testing for the corresponding germline mutations.</p><p><b>Methodology: </b> Retrospective review of the prospectively gathered clinical, pathological, and genetic records of all patients diagnosed with CRC aged 50 years and below between January 2001 and December 2007.</p><p><b>Results: </b> 244 patients aged 50 years or below with CRC were diagnosed in this period. 212 (86.9%) patients’ tumours underwent IHC, 147 of which had all 4 gene products studied. 30 (14.2%) tumours had LOE for one or more MMR proteins. 25/30 patients were offered genetic testing of whom 3 declined. So far 8 out of 16 patients tested have a confirmed germline mutation. Only 3/8 patients report a family history of CRC.</p><p><b>Conclusion: </b> Tumour IHC reveals LOE for one of the four MMR gene proteins in approximately 14% of young patients developing CRC and in half subsequent genetic testing will identify Lynch syndrome. A policy of mandatory testing is confirmed but audit of performance and appropriate referral is required.</p></abstract></abstractGroup></contentMeta></header></component></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>CR07*IMMUNOHISTOCHEMISTRY FOR LOSS OF EXPRESSION OF MISMATCH REPAIR GENE PROTEINS IN YOUNG PATIENTS WITH COLORECTAL CANCER: THE AUCKLAND EXPERIENCE</title></titleInfo><titleInfo type="abbreviated"><title>ANZ J. Surg. 2009; 79 (Suppl. 1)</title></titleInfo><titleInfo type="alternative" contentType="CDATA" lang="en"><title>CR07
*IMMUNOHISTOCHEMISTRY FOR LOSS OF EXPRESSION OF MISMATCH REPAIR GENE PROTEINS IN YOUNG PATIENTS WITH COLORECTAL CANCER: THE AUCKLAND EXPERIENCE</title></titleInfo><name type="personal"><namePart type="given">D. M.</namePart><namePart type="family">Wright</namePart><affiliation>Auckland City Hospital, Auckland, New Zealand</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">S.</namePart><namePart type="family">Parry</namePart><affiliation>Auckland City Hospital, Auckland, New Zealand</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">J.</namePart><namePart type="family">Arnold</namePart><affiliation>Auckland City Hospital, Auckland, New Zealand</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">I.</namePart><namePart type="family">Bissett</namePart><affiliation>Auckland City Hospital, Auckland, New Zealand</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">M.</namePart><namePart type="family">Hulme‐Moir</namePart><affiliation>Auckland City Hospital, Auckland, New Zealand</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">B.</namePart><namePart type="family">Parry</namePart><affiliation>Auckland City Hospital, Auckland, New Zealand</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="abstract" displayLabel="abstract"></genre><originInfo><publisher>Blackwell Publishing Asia</publisher><place><placeTerm type="text">Melbourne, Australia</placeTerm></place><dateIssued encoding="w3cdtf">2009-05</dateIssued><copyrightDate encoding="w3cdtf">2009</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><physicalDescription><internetMediaType>text/html</internetMediaType><extent unit="words">5149</extent></physicalDescription><abstract lang="en">Recently, in Australasia, a plea to screen all young patients with colorectal cancer (CRC) for Lynch syndrome has been advanced1. Since 2001 the three public hospitals in Auckland have had the policy of undertaking immunohistochemical (IHC) testing on the tumours of patients aged 50 years and under to detect loss of expression (LOE) of protein products for the mismatch repair (MMR) genes MLH1, MSH2, MSH6 and PMS2. Purpose: To determine (1) the completeness of patient capture, (2) the incidence of LOE by IHC of the 4 gene proteins, and (3) to correlate IHC results with those of subsequent genetic testing for the corresponding germline mutations. Methodology: Retrospective review of the prospectively gathered clinical, pathological, and genetic records of all patients diagnosed with CRC aged 50 years and below between January 2001 and December 2007. Results: 244 patients aged 50 years or below with CRC were diagnosed in this period. 212 (86.9%) patients’ tumours underwent IHC, 147 of which had all 4 gene products studied. 30 (14.2%) tumours had LOE for one or more MMR proteins. 25/30 patients were offered genetic testing of whom 3 declined. So far 8 out of 16 patients tested have a confirmed germline mutation. Only 3/8 patients report a family history of CRC. Conclusion: Tumour IHC reveals LOE for one of the four MMR gene proteins in approximately 14% of young patients developing CRC and in half subsequent genetic testing will identify Lynch syndrome. A policy of mandatory testing is confirmed but audit of performance and appropriate referral is required.</abstract><relatedItem type="host"><titleInfo><title>ANZ Journal of Surgery</title></titleInfo><genre type="journal">journal</genre><identifier type="ISSN">1445-1433</identifier><identifier type="eISSN">1445-2197</identifier><identifier type="DOI">10.1111/(ISSN)1445-2197</identifier><identifier type="PublisherID">ANS</identifier><part><date>2009</date><detail type="title"><title>Abstracts of the Royal Australasian College of Surgeons Annual Scientific Congress, 6–9 May 2009, Brisbane, Queensland, Australia</title></detail><detail type="volume"><caption>vol.</caption><number>79</number></detail><detail type="supplement"><caption>Suppl. no.</caption><number>s1</number></detail><extent unit="pages"><start>A10</start><end>A10</end><total>1</total></extent></part></relatedItem><identifier type="istex">C61AF7AD23BF14689F1B4649130DCC991B2F94A3</identifier><identifier type="DOI">10.1111/j.1445-2197.2009.04915_7.x</identifier><identifier type="ArticleID">ANS4915_7</identifier><accessCondition type="use and reproduction" contentType="copyright">Journal compilation © 2009 The Royal Australasian College of Surgeons</accessCondition><recordInfo><recordContentSource>WILEY</recordContentSource><recordOrigin>Blackwell Publishing Asia</recordOrigin></recordInfo></mods></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Ticri/CIDE/explor/OcrV1/Data/Istex/Corpus
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001174 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Istex/Corpus/biblio.hfd -nk 001174 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Ticri/CIDE
|area= OcrV1
|flux= Istex
|étape= Corpus
|type= RBID
|clé= ISTEX:C61AF7AD23BF14689F1B4649130DCC991B2F94A3
|texte= CR07*IMMUNOHISTOCHEMISTRY FOR LOSS OF EXPRESSION OF MISMATCH REPAIR GENE PROTEINS IN YOUNG PATIENTS WITH COLORECTAL CANCER: THE AUCKLAND EXPERIENCE
}}
| This area was generated with Dilib version V0.6.32. |  |