Sex‐specific alterations in NOS regulation of vascular function in aorta and mesenteric arteries from spontaneously hypertensive rats compared to Wistar Kyoto rats
Identifieur interne : 000088 ( Pmc/Corpus ); précédent : 000087; suivant : 000089Sex‐specific alterations in NOS regulation of vascular function in aorta and mesenteric arteries from spontaneously hypertensive rats compared to Wistar Kyoto rats
Auteurs : Analia S. Loria ; Krystal N. Brinson ; Brandon M. Fox ; Jennifer C. SullivanSource :
- Physiological Reports [ 2051-817X ] ; 2014.
Abstract
The present study tested the hypothesis that spontaneously hypertensive rats (SHR) have impaired nitric oxide synthase (NOS)‐mediated regulation of vascular function versus Wistar‐Kyoto rats (WKY). Aorta and small mesenteric arteries were studied from male and female SHR (M SHR and F SHR) and WKY (M WKY and F WKY). Phenylephrine (PE)‐induced vasoconstriction was greater in aorta of M SHR versus all others (
Url:
DOI: 10.14814/phy2.12125
PubMed: 25168874
PubMed Central: 4246578
Links to Exploration step
PMC:4246578Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Sex‐specific alterations in NOS regulation of vascular function in aorta and mesenteric arteries from spontaneously hypertensive rats compared to Wistar Kyoto rats</title>
<author><name sortKey="Loria, Analia S" sort="Loria, Analia S" uniqKey="Loria A" first="Analia S." last="Loria">Analia S. Loria</name>
<affiliation><nlm:aff>NONE</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Brinson, Krystal N" sort="Brinson, Krystal N" uniqKey="Brinson K" first="Krystal N." last="Brinson">Krystal N. Brinson</name>
<affiliation><nlm:aff>NONE</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Fox, Brandon M" sort="Fox, Brandon M" uniqKey="Fox B" first="Brandon M." last="Fox">Brandon M. Fox</name>
<affiliation><nlm:aff>NONE</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Sullivan, Jennifer C" sort="Sullivan, Jennifer C" uniqKey="Sullivan J" first="Jennifer C." last="Sullivan">Jennifer C. Sullivan</name>
<affiliation><nlm:aff>NONE</nlm:aff>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PMC</idno>
<idno type="pmid">25168874</idno>
<idno type="pmc">4246578</idno>
<idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4246578</idno>
<idno type="RBID">PMC:4246578</idno>
<idno type="doi">10.14814/phy2.12125</idno>
<date when="2014">2014</date>
<idno type="wicri:Area/Pmc/Corpus">000088</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000088</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Sex‐specific alterations in NOS regulation of vascular function in aorta and mesenteric arteries from spontaneously hypertensive rats compared to Wistar Kyoto rats</title>
<author><name sortKey="Loria, Analia S" sort="Loria, Analia S" uniqKey="Loria A" first="Analia S." last="Loria">Analia S. Loria</name>
<affiliation><nlm:aff>NONE</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Brinson, Krystal N" sort="Brinson, Krystal N" uniqKey="Brinson K" first="Krystal N." last="Brinson">Krystal N. Brinson</name>
<affiliation><nlm:aff>NONE</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Fox, Brandon M" sort="Fox, Brandon M" uniqKey="Fox B" first="Brandon M." last="Fox">Brandon M. Fox</name>
<affiliation><nlm:aff>NONE</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Sullivan, Jennifer C" sort="Sullivan, Jennifer C" uniqKey="Sullivan J" first="Jennifer C." last="Sullivan">Jennifer C. Sullivan</name>
<affiliation><nlm:aff>NONE</nlm:aff>
</affiliation>
</author>
</analytic>
<series><title level="j">Physiological Reports</title>
<idno type="eISSN">2051-817X</idno>
<imprint><date when="2014">2014</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass></textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en"><title>Abstract</title>
<p>The present study tested the hypothesis that spontaneously hypertensive rats (SHR) have impaired nitric oxide synthase (NOS)‐mediated regulation of vascular function versus Wistar‐Kyoto rats (WKY). Aorta and small mesenteric arteries were studied from male and female SHR (M SHR and F SHR) and WKY (M WKY and F WKY). Phenylephrine (PE)‐induced vasoconstriction was greater in aorta of M SHR versus all others (<italic>P</italic>
< 0.05); there were neither sex nor strain differences in PE contraction in mesenteric arteries. The NOS inhibitor l‐Nitro‐Arginine Methyl Ester (l‐NAME) increased PE‐induced vasoconstriction in all rats, although the increase was the least in male SHR (<italic>P</italic>
< 0.05), revealing a blunted vasoconstrictor buffering capacity of NOS. l‐NAME increased sensitivity to PE‐induced constriction only in mesenteric arteries of SHR, although, the maximal percent increase in contraction was comparable among groups. ACh‐induced relaxation was also less in aorta from M SHR versus all others (<italic>P</italic>
< 0.05). ACh relaxation was comparable among groups in mesenteric arteries, although SHR exhibited a greater NOS component to ACh‐induced relaxation than WKY. To gain mechanistic insight into sex and strain differences in vascular function, NOS activity and NOS3 protein expression were measured. Aortic NOS activity was comparable between groups and M SHR had greater NOS3 expression than M WKY. In contrast, although vascular function was largely maintained in mesenteric arteries of SHR, NOS activity was less in SHR versus WKY. In conclusion, M SHR exhibit a decrease in NOS regulation of vascular function compared to F SHR and WKY, although this is not mediated by decreases in NOS activity and/or expression.</p>
</div>
</front>
<back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Anishchenko, T G" uniqKey="Anishchenko T">T. G. Anishchenko</name>
</author>
<author><name sortKey="Igosheva, N B" uniqKey="Igosheva N">N. B. Igosheva</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Ballerio, R" uniqKey="Ballerio R">R. Ballerio</name>
</author>
<author><name sortKey="Gianazza, E" uniqKey="Gianazza E">E. Gianazza</name>
</author>
<author><name sortKey="Mussoni, L" uniqKey="Mussoni L">L. Mussoni</name>
</author>
<author><name sortKey="Miller, I" uniqKey="Miller I">I. Miller</name>
</author>
<author><name sortKey="Gelosa, P" uniqKey="Gelosa P">P. Gelosa</name>
</author>
<author><name sortKey="Guerrini, U" uniqKey="Guerrini U">U. Guerrini</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Baylis, C" uniqKey="Baylis C">C. Baylis</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Baylis, C" uniqKey="Baylis C">C. Baylis</name>
</author>
<author><name sortKey="Mitruka, B" uniqKey="Mitruka B">B. Mitruka</name>
</author>
<author><name sortKey="Deng, A" uniqKey="Deng A">A. Deng</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Brinson, K N" uniqKey="Brinson K">K. N. Brinson</name>
</author>
<author><name sortKey="Elmarakby, A A" uniqKey="Elmarakby A">A. A. Elmarakby</name>
</author>
<author><name sortKey="Tipton, A J" uniqKey="Tipton A">A. J. Tipton</name>
</author>
<author><name sortKey="Crislip, G R" uniqKey="Crislip G">G. R. Crislip</name>
</author>
<author><name sortKey="Yamamoto, T" uniqKey="Yamamoto T">T. Yamamoto</name>
</author>
<author><name sortKey="Baban, B" uniqKey="Baban B">B. Baban</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Chamiot Lerc, P" uniqKey="Chamiot Lerc P">P. Chamiot‐Clerc</name>
</author>
<author><name sortKey="Renaud, J F" uniqKey="Renaud J">J. F. Renaud</name>
</author>
<author><name sortKey="Safar, M E" uniqKey="Safar M">M. E. Safar</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Crews, J K" uniqKey="Crews J">J. K. Crews</name>
</author>
<author><name sortKey="Khalil, R A" uniqKey="Khalil R">R. A. Khalil</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Crews, J K" uniqKey="Crews J">J. K. Crews</name>
</author>
<author><name sortKey="Khalil, R A" uniqKey="Khalil R">R. A. Khalil</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Crews, J K" uniqKey="Crews J">J. K. Crews</name>
</author>
<author><name sortKey="Murphy, J G" uniqKey="Murphy J">J. G. Murphy</name>
</author>
<author><name sortKey="Khalil, R A" uniqKey="Khalil R">R. A. Khalil</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Dowell, F J" uniqKey="Dowell F">F. J. Dowell</name>
</author>
<author><name sortKey="Martin, W" uniqKey="Martin W">W. Martin</name>
</author>
<author><name sortKey="Dominiczak, A F" uniqKey="Dominiczak A">A. F. Dominiczak</name>
</author>
<author><name sortKey="Hamilton, C A" uniqKey="Hamilton C">C. A. Hamilton</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Forte, P" uniqKey="Forte P">P. Forte</name>
</author>
<author><name sortKey="Kneale, B J" uniqKey="Kneale B">B. J. Kneale</name>
</author>
<author><name sortKey="Milne, E" uniqKey="Milne E">E. Milne</name>
</author>
<author><name sortKey="Chowienczyk, P J" uniqKey="Chowienczyk P">P. J. Chowienczyk</name>
</author>
<author><name sortKey="Johnston, A" uniqKey="Johnston A">A. Johnston</name>
</author>
<author><name sortKey="Benjamin, N" uniqKey="Benjamin N">N. Benjamin</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Furchgott, R F" uniqKey="Furchgott R">R. F. Furchgott</name>
</author>
<author><name sortKey="Zawadzki, J V" uniqKey="Zawadzki J">J. V. Zawadzki</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Gamboa, A" uniqKey="Gamboa A">A. Gamboa</name>
</author>
<author><name sortKey="Shibao, C" uniqKey="Shibao C">C. Shibao</name>
</author>
<author><name sortKey="Diedrich, A" uniqKey="Diedrich A">A. Diedrich</name>
</author>
<author><name sortKey="Choi, L" uniqKey="Choi L">L. Choi</name>
</author>
<author><name sortKey="Pohar, B" uniqKey="Pohar B">B. Pohar</name>
</author>
<author><name sortKey="Jordan, J" uniqKey="Jordan J">J. Jordan</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Gerhard, M" uniqKey="Gerhard M">M. Gerhard</name>
</author>
<author><name sortKey="Ganz, P" uniqKey="Ganz P">P. Ganz</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Giachini, F R" uniqKey="Giachini F">F. R. Giachini</name>
</author>
<author><name sortKey="Lima, V V" uniqKey="Lima V">V. V. Lima</name>
</author>
<author><name sortKey="Filgueira, F P" uniqKey="Filgueira F">F. P. Filgueira</name>
</author>
<author><name sortKey="Dorrance, A M" uniqKey="Dorrance A">A. M. Dorrance</name>
</author>
<author><name sortKey="Carvalho, M H" uniqKey="Carvalho M">M. H. Carvalho</name>
</author>
<author><name sortKey="Fortes, Z B" uniqKey="Fortes Z">Z. B. Fortes</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Giles, T D" uniqKey="Giles T">T. D. Giles</name>
</author>
<author><name sortKey="Sander, G E" uniqKey="Sander G">G. E. Sander</name>
</author>
<author><name sortKey="Nossaman, B D" uniqKey="Nossaman B">B. D. Nossaman</name>
</author>
<author><name sortKey="Kadowitz, P J" uniqKey="Kadowitz P">P. J. Kadowitz</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Glushkovskaya Emyachkina, O V" uniqKey="Glushkovskaya Emyachkina O">O. V. Glushkovskaya‐Semyachkina</name>
</author>
<author><name sortKey="Anishchenko, T G" uniqKey="Anishchenko T">T. G. Anishchenko</name>
</author>
<author><name sortKey="Sindyakova, T A" uniqKey="Sindyakova T">T. A. Sindyakova</name>
</author>
<author><name sortKey="Leksina, O V" uniqKey="Leksina O">O. V. Leksina</name>
</author>
<author><name sortKey="Berdnikova, V A" uniqKey="Berdnikova V">V. A. Berdnikova</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Huang, A" uniqKey="Huang A">A. Huang</name>
</author>
<author><name sortKey="Sun, D" uniqKey="Sun D">D. Sun</name>
</author>
<author><name sortKey="Kaley, G" uniqKey="Kaley G">G. Kaley</name>
</author>
<author><name sortKey="Koller, A" uniqKey="Koller A">A. Koller</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Husken, B C" uniqKey="Husken B">B. C. Husken</name>
</author>
<author><name sortKey="Mertens, M J" uniqKey="Mertens M">M. J. Mertens</name>
</author>
<author><name sortKey="Pfaffendorf, M" uniqKey="Pfaffendorf M">M. Pfaffendorf</name>
</author>
<author><name sortKey="Van Zwieten, P A" uniqKey="Van Zwieten P">P. A. Van Zwieten</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Iliescu, R" uniqKey="Iliescu R">R. Iliescu</name>
</author>
<author><name sortKey="Yanes, L L" uniqKey="Yanes L">L. L. Yanes</name>
</author>
<author><name sortKey="Bell, W" uniqKey="Bell W">W. Bell</name>
</author>
<author><name sortKey="Dwyer, T" uniqKey="Dwyer T">T. Dwyer</name>
</author>
<author><name sortKey="Baltatu, O C" uniqKey="Baltatu O">O. C. Baltatu</name>
</author>
<author><name sortKey="Reckelhoff, J F" uniqKey="Reckelhoff J">J. F. Reckelhoff</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Iliescu, R" uniqKey="Iliescu R">R. Iliescu</name>
</author>
<author><name sortKey="Cucchiarelli, V E" uniqKey="Cucchiarelli V">V. E. Cucchiarelli</name>
</author>
<author><name sortKey="Yanes, L L" uniqKey="Yanes L">L. L. Yanes</name>
</author>
<author><name sortKey="Iles, J W" uniqKey="Iles J">J. W. Iles</name>
</author>
<author><name sortKey="Reckelhoff, J F" uniqKey="Reckelhoff J">J. F. Reckelhoff</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Kahonen, M" uniqKey="Kahonen M">M. Kahonen</name>
</author>
<author><name sortKey="Tolvanen, J P" uniqKey="Tolvanen J">J. P. Tolvanen</name>
</author>
<author><name sortKey="Sallinen, K" uniqKey="Sallinen K">K. Sallinen</name>
</author>
<author><name sortKey="Wu, " uniqKey="Wu ">. Wu</name>
</author>
<author><name sortKey="Porsti, I" uniqKey="Porsti I">I. Porsti</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Kang, K T" uniqKey="Kang K">K. T. Kang</name>
</author>
<author><name sortKey="Sullivan, J C" uniqKey="Sullivan J">J. C. Sullivan</name>
</author>
<author><name sortKey="Sasser, J M" uniqKey="Sasser J">J. M. Sasser</name>
</author>
<author><name sortKey="Imig, J D" uniqKey="Imig J">J. D. Imig</name>
</author>
<author><name sortKey="Pollock, J S" uniqKey="Pollock J">J. S. Pollock</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Kauser, K" uniqKey="Kauser K">K. Kauser</name>
</author>
<author><name sortKey="Rubanyi, G M" uniqKey="Rubanyi G">G. M. Rubanyi</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Kauser, K" uniqKey="Kauser K">K. Kauser</name>
</author>
<author><name sortKey="Rubanyi, G M" uniqKey="Rubanyi G">G. M. Rubanyi</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Kerr, S" uniqKey="Kerr S">S. Kerr</name>
</author>
<author><name sortKey="Brosnan, M J" uniqKey="Brosnan M">M. J. Brosnan</name>
</author>
<author><name sortKey="Mcintyre, M" uniqKey="Mcintyre M">M. McIntyre</name>
</author>
<author><name sortKey="Reid, J L" uniqKey="Reid J">J. L. Reid</name>
</author>
<author><name sortKey="Dominiczak, A F" uniqKey="Dominiczak A">A. F. Dominiczak</name>
</author>
<author><name sortKey="Hamilton, C A" uniqKey="Hamilton C">C. A. Hamilton</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Loria, A S" uniqKey="Loria A">A. S. Loria</name>
</author>
<author><name sortKey="Kang, K T" uniqKey="Kang K">K. T. Kang</name>
</author>
<author><name sortKey="Pollock, D M" uniqKey="Pollock D">D. M. Pollock</name>
</author>
<author><name sortKey="Pollock, J S" uniqKey="Pollock J">J. S. Pollock</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Makino, A" uniqKey="Makino A">A. Makino</name>
</author>
<author><name sortKey="Ohuchi, K" uniqKey="Ohuchi K">K. Ohuchi</name>
</author>
<author><name sortKey="Kamata, K" uniqKey="Kamata K">K. Kamata</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Maris, M E" uniqKey="Maris M">M. E. Maris</name>
</author>
<author><name sortKey="Melchert, R B" uniqKey="Melchert R">R. B. Melchert</name>
</author>
<author><name sortKey="Joseph, J" uniqKey="Joseph J">J. Joseph</name>
</author>
<author><name sortKey="Kennedy, R H" uniqKey="Kennedy R">R. H. Kennedy</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Matrougui, K" uniqKey="Matrougui K">K. Matrougui</name>
</author>
<author><name sortKey="Maclouf, J" uniqKey="Maclouf J">J. Maclouf</name>
</author>
<author><name sortKey="Levy, B I" uniqKey="Levy B">B. I. Levy</name>
</author>
<author><name sortKey="Henrion, D" uniqKey="Henrion D">D. Henrion</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Mcintyre, M" uniqKey="Mcintyre M">M. McIntyre</name>
</author>
<author><name sortKey="Hamilton, C A" uniqKey="Hamilton C">C. A. Hamilton</name>
</author>
<author><name sortKey="Rees, D D" uniqKey="Rees D">D. D. Rees</name>
</author>
<author><name sortKey="Reid, J L" uniqKey="Reid J">J. L. Reid</name>
</author>
<author><name sortKey="Dominiczak, A F" uniqKey="Dominiczak A">A. F. Dominiczak</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Miller, V M" uniqKey="Miller V">V. M. Miller</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Murphy, J G" uniqKey="Murphy J">J. G. Murphy</name>
</author>
<author><name sortKey="Khalil, R A" uniqKey="Khalil R">R. A. Khalil</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Nyborg, N C" uniqKey="Nyborg N">N. C. Nyborg</name>
</author>
<author><name sortKey="Bevan, J A" uniqKey="Bevan J">J. A. Bevan</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Orshal, J M" uniqKey="Orshal J">J. M. Orshal</name>
</author>
<author><name sortKey="Khalil, R A" uniqKey="Khalil R">R. A. Khalil</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Piech, A" uniqKey="Piech A">A. Piech</name>
</author>
<author><name sortKey="Dessy, C" uniqKey="Dessy C">C. Dessy</name>
</author>
<author><name sortKey="Havaux, X" uniqKey="Havaux X">X. Havaux</name>
</author>
<author><name sortKey="Feron, O" uniqKey="Feron O">O. Feron</name>
</author>
<author><name sortKey="Balligand, J L" uniqKey="Balligand J">J. L. Balligand</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Ribeiro, M O" uniqKey="Ribeiro M">M. O. Ribeiro</name>
</author>
<author><name sortKey="Antunes, E" uniqKey="Antunes E">E. Antunes</name>
</author>
<author><name sortKey="De Nucci, G" uniqKey="De Nucci G">G. de Nucci</name>
</author>
<author><name sortKey="Lovisolo, S M" uniqKey="Lovisolo S">S. M. Lovisolo</name>
</author>
<author><name sortKey="Zatz, R" uniqKey="Zatz R">R. Zatz</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Sader, M A" uniqKey="Sader M">M. A. Sader</name>
</author>
<author><name sortKey="Celermajer, D S" uniqKey="Celermajer D">D. S. Celermajer</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Sasser, J M" uniqKey="Sasser J">J. M. Sasser</name>
</author>
<author><name sortKey="Sullivan, J C" uniqKey="Sullivan J">J. C. Sullivan</name>
</author>
<author><name sortKey="Elmarakby, A A" uniqKey="Elmarakby A">A. A. Elmarakby</name>
</author>
<author><name sortKey="Kemp, B E" uniqKey="Kemp B">B. E. Kemp</name>
</author>
<author><name sortKey="Pollock, D M" uniqKey="Pollock D">D. M. Pollock</name>
</author>
<author><name sortKey="Pollock, J S" uniqKey="Pollock J">J. S. Pollock</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Silva Ntonialli, M M" uniqKey="Silva Ntonialli M">M. M. Silva‐Antonialli</name>
</author>
<author><name sortKey="Fortes, Z B" uniqKey="Fortes Z">Z. B. Fortes</name>
</author>
<author><name sortKey="Carvalho, M H" uniqKey="Carvalho M">M. H. Carvalho</name>
</author>
<author><name sortKey="Scivoletto, R" uniqKey="Scivoletto R">R. Scivoletto</name>
</author>
<author><name sortKey="Nigro, D" uniqKey="Nigro D">D. Nigro</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Skaug, E A" uniqKey="Skaug E">E. A. Skaug</name>
</author>
<author><name sortKey="Aspenes, S T" uniqKey="Aspenes S">S. T. Aspenes</name>
</author>
<author><name sortKey="Oldervoll, L" uniqKey="Oldervoll L">L. Oldervoll</name>
</author>
<author><name sortKey="Morkedal, B" uniqKey="Morkedal B">B. Morkedal</name>
</author>
<author><name sortKey="Vatten, L" uniqKey="Vatten L">L. Vatten</name>
</author>
<author><name sortKey="Wisloff, U" uniqKey="Wisloff U">U. Wisloff</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Sullivan, J C" uniqKey="Sullivan J">J. C. Sullivan</name>
</author>
<author><name sortKey="Pollock, D M" uniqKey="Pollock D">D. M. Pollock</name>
</author>
<author><name sortKey="Pollock, J S" uniqKey="Pollock J">J. S. Pollock</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Sullivan, J C" uniqKey="Sullivan J">J. C. Sullivan</name>
</author>
<author><name sortKey="Loomis, E D" uniqKey="Loomis E">E. D. Loomis</name>
</author>
<author><name sortKey="Collins, M" uniqKey="Collins M">M. Collins</name>
</author>
<author><name sortKey="Imig, J D" uniqKey="Imig J">J. D. Imig</name>
</author>
<author><name sortKey="Inscho, E W" uniqKey="Inscho E">E. W. Inscho</name>
</author>
<author><name sortKey="Pollock, J S" uniqKey="Pollock J">J. S. Pollock</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Sullivan, J C" uniqKey="Sullivan J">J. C. Sullivan</name>
</author>
<author><name sortKey="Semprun Rieto, L" uniqKey="Semprun Rieto L">L. Semprun‐Prieto</name>
</author>
<author><name sortKey="Boesen, E I" uniqKey="Boesen E">E. I. Boesen</name>
</author>
<author><name sortKey="Pollock, D M" uniqKey="Pollock D">D. M. Pollock</name>
</author>
<author><name sortKey="Pollock, J S" uniqKey="Pollock J">J. S. Pollock</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Sullivan, J C" uniqKey="Sullivan J">J. C. Sullivan</name>
</author>
<author><name sortKey="Pardieck, J L" uniqKey="Pardieck J">J. L. Pardieck</name>
</author>
<author><name sortKey="Doran, D" uniqKey="Doran D">D. Doran</name>
</author>
<author><name sortKey="Zhang, Y" uniqKey="Zhang Y">Y. Zhang</name>
</author>
<author><name sortKey="She, J X" uniqKey="She J">J. X. She</name>
</author>
<author><name sortKey="Pollock, J S" uniqKey="Pollock J">J. S. Pollock</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Sullivan, J C" uniqKey="Sullivan J">J. C. Sullivan</name>
</author>
<author><name sortKey="Pardieck, J L" uniqKey="Pardieck J">J. L. Pardieck</name>
</author>
<author><name sortKey="Hyndman, K A" uniqKey="Hyndman K">K. A. Hyndman</name>
</author>
<author><name sortKey="Pollock, J S" uniqKey="Pollock J">J. S. Pollock</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Sun, T" uniqKey="Sun T">T. Sun</name>
</author>
<author><name sortKey="Wang, J" uniqKey="Wang J">J. Wang</name>
</author>
<author><name sortKey="Huang, L H" uniqKey="Huang L">L. H. Huang</name>
</author>
<author><name sortKey="Cao, Y X" uniqKey="Cao Y">Y. X. Cao</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Takamura, Y" uniqKey="Takamura Y">Y. Takamura</name>
</author>
<author><name sortKey="Shimokawa, H" uniqKey="Shimokawa H">H. Shimokawa</name>
</author>
<author><name sortKey="Zhao, H" uniqKey="Zhao H">H. Zhao</name>
</author>
<author><name sortKey="Igarashi, H" uniqKey="Igarashi H">H. Igarashi</name>
</author>
<author><name sortKey="Egashira, K" uniqKey="Egashira K">K. Egashira</name>
</author>
<author><name sortKey="Takeshita, A" uniqKey="Takeshita A">A. Takeshita</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Tipton, A J" uniqKey="Tipton A">A. J. Tipton</name>
</author>
<author><name sortKey="Baban, B" uniqKey="Baban B">B. Baban</name>
</author>
<author><name sortKey="Sullivan, J C" uniqKey="Sullivan J">J. C. Sullivan</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Toot, J D" uniqKey="Toot J">J. D. Toot</name>
</author>
<author><name sortKey="Reho, J J" uniqKey="Reho J">J. J. Reho</name>
</author>
<author><name sortKey="Novak, J" uniqKey="Novak J">J. Novak</name>
</author>
<author><name sortKey="Dunphy, G" uniqKey="Dunphy G">G. Dunphy</name>
</author>
<author><name sortKey="Ely, D L" uniqKey="Ely D">D. L. Ely</name>
</author>
<author><name sortKey="Ramirez, R J" uniqKey="Ramirez R">R. J. Ramirez</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Tschudi, M R" uniqKey="Tschudi M">M. R. Tschudi</name>
</author>
<author><name sortKey="Mesaros, S" uniqKey="Mesaros S">S. Mesaros</name>
</author>
<author><name sortKey="Luscher, T F" uniqKey="Luscher T">T. F. Luscher</name>
</author>
<author><name sortKey="Malinski, T" uniqKey="Malinski T">T. Malinski</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Vaziri, N D" uniqKey="Vaziri N">N. D. Vaziri</name>
</author>
<author><name sortKey="Ni, Z" uniqKey="Ni Z">Z. Ni</name>
</author>
<author><name sortKey="Oveisi, F" uniqKey="Oveisi F">F. Oveisi</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Wiinberg, N" uniqKey="Wiinberg N">N. Wiinberg</name>
</author>
<author><name sortKey="Hoegholm, A" uniqKey="Hoegholm A">A. Hoegholm</name>
</author>
<author><name sortKey="Christensen, H R" uniqKey="Christensen H">H. R. Christensen</name>
</author>
<author><name sortKey="Bang, L E" uniqKey="Bang L">L. E. Bang</name>
</author>
<author><name sortKey="Mikkelsen, K L" uniqKey="Mikkelsen K">K. L. Mikkelsen</name>
</author>
<author><name sortKey="Nielsen, P E" uniqKey="Nielsen P">P. E. Nielsen</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Yanes, L L" uniqKey="Yanes L">L. L. Yanes</name>
</author>
<author><name sortKey="Romero, D G" uniqKey="Romero D">D. G. Romero</name>
</author>
<author><name sortKey="Cucchiarelli, V E" uniqKey="Cucchiarelli V">V. E. Cucchiarelli</name>
</author>
<author><name sortKey="Fortepiani, L A" uniqKey="Fortepiani L">L. A. Fortepiani</name>
</author>
<author><name sortKey="Gomez Anchez, C E" uniqKey="Gomez Anchez C">C. E. Gomez‐Sanchez</name>
</author>
<author><name sortKey="Santacruz, F" uniqKey="Santacruz F">F. Santacruz</name>
</author>
</analytic>
</biblStruct>
</listBibl>
</div1>
</back>
</TEI>
<pmc article-type="research-article"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Physiol Rep</journal-id>
<journal-id journal-id-type="iso-abbrev">Physiol Rep</journal-id>
<journal-id journal-id-type="hwp">physreports</journal-id>
<journal-id journal-id-type="publisher-id">phy2</journal-id>
<journal-title-group><journal-title>Physiological Reports</journal-title>
</journal-title-group>
<issn pub-type="epub">2051-817X</issn>
<publisher><publisher-name>Wiley Periodicals, Inc.</publisher-name>
</publisher>
</journal-meta>
<article-meta><article-id pub-id-type="pmid">25168874</article-id>
<article-id pub-id-type="pmc">4246578</article-id>
<article-id pub-id-type="publisher-id">phy212125</article-id>
<article-id pub-id-type="doi">10.14814/phy2.12125</article-id>
<article-categories><subj-group subj-group-type="heading"><subject>Original Research</subject>
</subj-group>
</article-categories>
<title-group><article-title>Sex‐specific alterations in NOS regulation of vascular function in aorta and mesenteric arteries from spontaneously hypertensive rats compared to Wistar Kyoto rats</article-title>
</title-group>
<contrib-group><contrib contrib-type="author"><name><surname>Loria</surname>
<given-names>Analia S.</given-names>
</name>
<xref ref-type="aff" rid="au1"></xref>
</contrib>
<contrib contrib-type="author"><name><surname>Brinson</surname>
<given-names>Krystal N.</given-names>
</name>
<xref ref-type="aff" rid="au2"></xref>
</contrib>
<contrib contrib-type="author"><name><surname>Fox</surname>
<given-names>Brandon M.</given-names>
</name>
<xref ref-type="aff" rid="au2"></xref>
</contrib>
<contrib contrib-type="author"><name><surname>Sullivan</surname>
<given-names>Jennifer C.</given-names>
</name>
<xref ref-type="aff" rid="au2"></xref>
</contrib>
<aff id="au1"><label>1</label>
<institution>Department of Pharmacology and Nutritional Sciences, University of Kentucky,</institution>
<addr-line>St. Lexington, Kentucky</addr-line>
</aff>
<aff id="au2"><label>2</label>
<institution>Department of Physiology, Georgia Regents University,</institution>
<addr-line>Augusta, Georgia</addr-line>
</aff>
</contrib-group>
<author-notes><corresp id="cor1"><bold>Correspondence</bold>
Analia S. Loria, 900 South Limestone St. C.T. Wethington Building, room 562, Lexington, KY 40536.Tel: 859‐218‐1414Fax: 859‐237‐3646E‐mail: <email>analia.loria@uky.edu</email>
</corresp>
</author-notes>
<pub-date pub-type="collection"><month>8</month>
<year>2014</year>
</pub-date>
<pub-date pub-type="epub"><day>28</day>
<month>8</month>
<year>2014</year>
</pub-date>
<volume>2</volume>
<issue>8</issue>
<elocation-id>e12125</elocation-id>
<history><date date-type="received"><day>15</day>
<month>4</month>
<year>2014</year>
</date>
<date date-type="rev-recd"><day>29</day>
<month>7</month>
<year>2014</year>
</date>
<date date-type="accepted"><day>30</day>
<month>7</month>
<year>2014</year>
</date>
</history>
<permissions><copyright-statement>© 2014 The Authors. <italic>Physiological Reports</italic>
published by Wiley Periodicals, Inc. on behalf of the American Physiological Society and The Physiological Society.
</copyright-statement>
<copyright-year>2014</copyright-year>
<license license-type="open-access" xlink:href="http://creativecommons.org/licenses/by/3.0/"><license-p>This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.</license-p>
</license>
</permissions>
<abstract><title>Abstract</title>
<p>The present study tested the hypothesis that spontaneously hypertensive rats (SHR) have impaired nitric oxide synthase (NOS)‐mediated regulation of vascular function versus Wistar‐Kyoto rats (WKY). Aorta and small mesenteric arteries were studied from male and female SHR (M SHR and F SHR) and WKY (M WKY and F WKY). Phenylephrine (PE)‐induced vasoconstriction was greater in aorta of M SHR versus all others (<italic>P</italic>
< 0.05); there were neither sex nor strain differences in PE contraction in mesenteric arteries. The NOS inhibitor l‐Nitro‐Arginine Methyl Ester (l‐NAME) increased PE‐induced vasoconstriction in all rats, although the increase was the least in male SHR (<italic>P</italic>
< 0.05), revealing a blunted vasoconstrictor buffering capacity of NOS. l‐NAME increased sensitivity to PE‐induced constriction only in mesenteric arteries of SHR, although, the maximal percent increase in contraction was comparable among groups. ACh‐induced relaxation was also less in aorta from M SHR versus all others (<italic>P</italic>
< 0.05). ACh relaxation was comparable among groups in mesenteric arteries, although SHR exhibited a greater NOS component to ACh‐induced relaxation than WKY. To gain mechanistic insight into sex and strain differences in vascular function, NOS activity and NOS3 protein expression were measured. Aortic NOS activity was comparable between groups and M SHR had greater NOS3 expression than M WKY. In contrast, although vascular function was largely maintained in mesenteric arteries of SHR, NOS activity was less in SHR versus WKY. In conclusion, M SHR exhibit a decrease in NOS regulation of vascular function compared to F SHR and WKY, although this is not mediated by decreases in NOS activity and/or expression.</p>
</abstract>
<abstract abstract-type="precis"><p>The present study tested the hypothesis that spontaneously hypertensive rats (SHR) have impaired nitric oxide synthase (NOS)‐mediated regulation of vascular function versus Wistar‐Kyoto rats (WKY). Aorta and small mesenteric arteries were studied from male and female SHR and WKY. Male SHR showed a decreased NOS regulation of vascular function compared to F SHR and WKY, although this was not mediated by decreases in NOS activity and/or expression.</p>
</abstract>
<kwd-group><kwd>NOS activity</kwd>
<kwd>NOS expression</kwd>
<kwd>phenylephrine</kwd>
<kwd>SHR</kwd>
</kwd-group>
</article-meta>
</front>
<body><sec sec-type="other"><title>Introduction</title>
<p>Men have a greater incidence and severity of cardiovascular disease compared to women until menopause (Gerhard and Ganz <xref rid="b14" ref-type="bibr">1995</xref>
; Wiinberg et al. <xref rid="b54" ref-type="bibr">1995</xref>
). A similar sex disparity in cardiovascular disease is seen in a number of experimental animal models of hypertension, including spontaneously hypertensive rats (SHR) (Kauser and Rubanyi <xref rid="b26" ref-type="bibr">1995</xref>
; Iliescu et al. <xref rid="b21" ref-type="bibr">2006</xref>
). SHR are a genetic model of hypertension and, similar to the human population, male SHR have a more rapid increase in blood pressure over time compared to female SHR. Although the molecular mechanisms responsible for sex differences in cardiovascular health and function are still unclear, the nitric oxide (NO)/NO synthase (NOS) pathway has been implicated in humans (Forte et al. <xref rid="b11" ref-type="bibr">1998</xref>
; Sader and Celermajer <xref rid="b39" ref-type="bibr">2002</xref>
) and rats (Baylis et al. <xref rid="b4" ref-type="bibr">1992</xref>
; Ribeiro et al. <xref rid="b38" ref-type="bibr">1992</xref>
; Kauser and Rubanyi <xref rid="b25" ref-type="bibr">1994</xref>
, Gamboa et al. <xref rid="b13" ref-type="bibr">2007</xref>
) and we recently published that female SHR are more dependent on NOS to maintain their blood pressure than males (Brinson et al. <xref rid="b5" ref-type="bibr">2013</xref>
).</p>
<p>NO is a key molecule regulating endothelial and vascular function (Forte et al. <xref rid="b11" ref-type="bibr">1998</xref>
; Vaziri et al. <xref rid="b53" ref-type="bibr">1998</xref>
). In particular, loss of NOS‐mediated control of vascular function contributes to both the development and progression of cardiovascular disease (Anishchenko and Igosheva <xref rid="b1" ref-type="bibr">1992</xref>
; McIntyre et al. <xref rid="b32" ref-type="bibr">1997</xref>
; Sader and Celermajer <xref rid="b39" ref-type="bibr">2002</xref>
; Orshal and Khalil <xref rid="b36" ref-type="bibr">2004</xref>
). It is also well established that there are sex differences in NO bioavailability, with females having greater levels of NO than males (Forte et al. <xref rid="b11" ref-type="bibr">1998</xref>
; Glushkovskaya‐Semyachkina et al. <xref rid="b17" ref-type="bibr">2006</xref>
; Sullivan et al. <xref rid="b47" ref-type="bibr">2010</xref>
) and numerous studies have demonstrated that greater NO in females contributes to maintaining cardiovascular function with age and disease relative to males (McIntyre et al. <xref rid="b32" ref-type="bibr">1997</xref>
; Miller <xref rid="b33" ref-type="bibr">2010</xref>
; Baylis <xref rid="b3" ref-type="bibr">2012</xref>
). However, whether there is differential NOS regulation of vasoconstriction and endothelial function in small and large arteries between males and females with normal blood pressure or hypertension has not been fully determined.</p>
<p>The impact of NOS on vascular function may be dependent, in part, on the size and physiological role of the artery. Decreases in NO bioavailability and NOS‐mediated vasorelaxation are commonly noted in conduit arteries from male animal models of hypertension, although we previously reported that NOS‐mediated vasorelaxation was maintained in small arteries from male hypertensive rats (Sasser et al. <xref rid="b40" ref-type="bibr">2004</xref>
; Kang et al. <xref rid="b24" ref-type="bibr">2007</xref>
). This finding is despite the fact that we and others have previously shown that endothelial NOS (NOS3; eNOS) protein expression is unchanged or even increased in the vasculature in hypertensive male animal models including SHR (Vaziri et al. <xref rid="b53" ref-type="bibr">1998</xref>
; Piech et al. <xref rid="b37" ref-type="bibr">2003</xref>
; Sun et al. <xref rid="b48" ref-type="bibr">2013</xref>
).</p>
<p>Therefore, the present study was designed to test the hypothesis that SHR have impaired NOS‐mediated regulation of vascular function (both constrictive and vasorelaxant) and reduced NOS activity and expression versus Wistar‐Kyoto rats (WKY). However, we further hypothesize that female SHR will maintain greater NOS‐mediated control of vascular function relative to males due to greater levels of NO in female SHR. Studies employed aorta, a conduit artery, and small mesenteric arteries and the relative impact of NOS on both vasoconstriction and vasodilation were measured in male and female SHR and WKY.</p>
</sec>
<sec sec-type="methods"><title>Methods</title>
<sec><title>Animals</title>
<p>Male and female SHR and WKY (Harlan Laboratories, Indianapolis, IN) were studied. All experiments were conducted in accordance with the National Institutes of Health Guide for the Care and Use of Laboratory Animals, European Union Directive 2010/63/EU for animal experiments and approved and monitored by the Georgia Regents University IACUC. Rats were housed in temperature and humidity‐controlled, light‐cycled quarters and maintained on a standard rodent diet (Teklad 8604, Madison, WI). At 12–13 weeks of age, rats were anesthetized with ketamine/xylazine (50 mg/kg and 6 mg/kg ip, respectively; Phoenix Pharmaceuticals, St. Joseph, MO) and thoracic aortas and third‐order mesenteric arteries were isolated.</p>
</sec>
<sec><title>Vascular reactivity</title>
<p>Thoracic aortas were cleaned of adherent adipose tissue, cut into concentric rings, and mounted on pins for wire myography (Danish Myo Technology A/S, Aarhus, Denmark) as previously described (Sullivan et al. <xref rid="b44" ref-type="bibr">2004</xref>
; Loria et al. <xref rid="b28" ref-type="bibr">2011</xref>
). Similarly, third‐order mesenteric arteries were isolated, cleaned of adherent adipose tissue, cut into segments, and mounted on wires for myography as previously described (Sullivan et al. <xref rid="b44" ref-type="bibr">2004</xref>
). Aortas preload tension was fixed to 28 mN, while mesenteric arteries preload tension was 4 mN. Vessel segments were equilibrated for 30 min before the viability of the vessel was determined by a robust vasoconstrictor response to 1 <italic>μ</italic>
mol/L phenylephrine (PE) followed by vasorelaxation to 10 <italic>μ</italic>
mol/L acetylcholine (ACh); only arteries that relaxed at least 80% of the maximal PE‐induced constriction were included in the study. Drugs were rinsed out and the vessels were allowed to reequilibrate for 30 min. Vasoconstriction was assessed by performing cumulative concentration–response (CCR) curves to the <italic>α</italic>
‐adrenergic agonist PE (1 × 10<sup>−9</sup>
–3 × 10<sup>−5</sup>
mol/L) in the absence or presence of the nonselective NOS inhibitor l‐Nitro‐Arginine Methyl Ester (l‐NAME, 15 min preincubation at 100 <italic>μ</italic>
mol/L, Sigma). The ability of NO to attenuate PE‐induced constriction, or the vasoconstrictor buffering capacity of NOS (Loria et al. <xref rid="b28" ref-type="bibr">2011</xref>
), was calculated as the delta of area under the curve (AUC, % increase in force × mol/L), determined by the difference in PE‐induced AUC ± l‐NAME. Vessel segments were then rinsed, allowed to reequilibrate and CCR curves were performed to KCl (8 × 10<sup>−3</sup>
to 100 × 10<sup>−3</sup>
mol/L) in the same artery segments. Vasoconstriction data are presented as percent increase in force from baseline tension. Endothelial‐dependent relaxation was also assessed in aortic and small mesenteric artery segments following preconstriction with 1 <italic>μ</italic>
mol/L or 2 <italic>μ</italic>
mol/L PE, respectively, followed by CCR curves to acetylcholine (ACh; 1 × 10<sup>−9</sup>
–1 × 10<sup>−5</sup>
mol/L) in the absence or presence of the l‐NAME (15 min preincubation at 100 <italic>μ</italic>
mol/L). Accordingly, delta AUC (% relaxation × mol/L) was determined by the difference in ACh‐induced AUC ± l‐NAME. There were no differences in the level of precontraction between the different rat groups. CCR curves were then performed to the endothelium‐independent vasodilator sodium nitroprusside (SNP; 100 pmol/L to 31.6 <italic>μ</italic>
mol/L) in the same artery segments. Vasorelaxation data are presented as percent relaxation from PE‐induced constriction. For all CCR, maximum response and sensitivity to the vasoactive agonists, pD<sub>2</sub>
(‐logEC50), were calculated.</p>
</sec>
<sec><title>NOS activity assay</title>
<p>Vascular tissue (whole aorta and mesenteric arterial beds) were isolated from additional rats and homogenized as previously described (Sullivan et al. <xref rid="b47" ref-type="bibr">2010</xref>
). Briefly, tissue was snap‐frozen and homogenized in cold homogenization buffer (20.0 mmol/L Tris·HCl pH 7.4, 137.0 mmol/L NaCl, 10% glycerol, and 1% NP40) containing protease inhibitors (1 mmol/L PMSF, 1 <italic>μ</italic>
mol/L pepstatin A, 2 <italic>μ</italic>
mol/L leupeptin, and 0.1% aprotinin). To determine total NOS enzymatic activity, tissue homogenates were incubated with [<sup>3</sup>
H]arginine (10 <italic>μ</italic>
mol/L final arginine, 71 Ci/mmol) in the presence of excess cofactors, as previously described, in a final volume of 50 <italic>μ</italic>
L for 30 min at room temperature (Sullivan et al. <xref rid="b47" ref-type="bibr">2010</xref>
; Loria et al. <xref rid="b28" ref-type="bibr">2011</xref>
). NOS activity was normalized to total protein concentration and expressed as pmoles citrulline/mg protein/30 min. Protein concentrations were determined by standard Bradford assay (Bio‐Rad, Hercules, CA) with the use of BSA as the standard.</p>
</sec>
<sec><title>Western blot analysis</title>
<p>Western blotting was performed as previously described using the same vessel homogenates that were used in the NOS activity measurement (Sullivan et al. <xref rid="b43" ref-type="bibr">2002</xref>
, <xref rid="b44" ref-type="bibr">2004</xref>
). Two‐color immunoblots were analyzed using polyclonal primary antibodies to NOS3 (1:500; BD Biosciences, San Jose, CA) and <italic>β</italic>
‐actin (A1978, 1:10,000; Sigma, St Louis, MO). After incubation with secondary antibody (IRDye 800‐conjugated affinity‐purified anti‐mouse IgG, 1:2,000 or AlexaFluor 680‐conjugated affinity‐purified anti‐rabbit IgG, 1:2,000), the blot was scanned and the intensity of specific bands were quantified using the Odyssey Infrared Imaging System (LI‐COR Biosciences, Lincoln, NE). Densitometric results were reported normalized to <italic>β</italic>
‐actin.</p>
</sec>
<sec><title>Data analysis</title>
<p>All values are expressed as mean ± SEM. Percent increase in force or relaxation in arteries, pD<sub>2</sub>
values, vasoconstrictor buffering capacity of NOS, and NOS activity in male and female SHR and WKY were compared using a Two‐way ANOVA with Bonferroni's post hoc performed using GraphPad Prism version 5.01 for Windows (GraphPad Software, San Diego, CA). NOS3 protein expression maximal response and pD<sub>2</sub>
values in control and l‐NAME‐treated vessels within each group were compared using a Student's <italic>t</italic>
‐test. For all comparisons, a value of <italic>P</italic>
< 0.05 was considered statistically significant.</p>
</sec>
</sec>
<sec sec-type="other"><title>Results</title>
<sec><title>Sex and strain differences in PE‐induced constriction and NOS capacity</title>
<p>Alpha‐adrenergic‐induced vasoconstriction was assessed in isolated aorta and mesenteric arteries from male and female SHR and WKY. PE‐induced vasoconstriction was not significantly different in aortic rings from male and female WKY (<xref ref-type="table" rid="tbl01">Table 1</xref>
and Fig. <xref ref-type="fig" rid="fig01">1</xref>
A). However, PE‐induced vasoconstriction was greater in aortic rings from male SHR compared with both female SHR and male and female WKY. PE‐induced constriction was comparable in female SHR and WKY (effect of sex = <italic>P</italic>
< 0.05; effect of strain = NS; interaction = <italic>P</italic>
< 0.05). Additional studies assessed the ability of basal NO levels to attenuate PE‐induced constriction in the aorta. Preincubation with l‐NAME increased maximal constriction to PE in all groups (Fig. <xref ref-type="fig" rid="fig01">1</xref>
B). The individual comparisons are depicted in panel 1C. Vasoconstrictor buffering capacity of NOS was significantly less in male SHR than in all other groups (effect of sex = NS; effect of strain = NS; interaction <italic>P</italic>
< 0.05, Fig. <xref ref-type="fig" rid="fig01">1</xref>
D).</p>
<table-wrap id="tbl01" position="float"><label>Table 1.</label>
<caption><p>Sensitivity and maximal responses to PE, ACh, SNP and KCl in absence or presence of a total nitric oxide synthase (NOS) inhibitor (l‐NAME).</p>
</caption>
<table frame="hsides" rules="groups"><thead><tr><th align="left" rowspan="2" colspan="1"></th>
<th align="center" colspan="2" rowspan="1">Male WKY</th>
<th align="center" colspan="2" rowspan="1">Male SHR</th>
<th align="center" colspan="2" rowspan="1">Female WKY</th>
<th align="center" colspan="2" rowspan="1">Females SHR</th>
</tr>
<tr><th align="center" rowspan="1" colspan="1">Aorta</th>
<th align="center" rowspan="1" colspan="1">Mesenteric</th>
<th align="center" rowspan="1" colspan="1">Aorta</th>
<th align="center" rowspan="1" colspan="1">Mesenteric</th>
<th align="center" rowspan="1" colspan="1">Aorta</th>
<th align="center" rowspan="1" colspan="1">Mesenteric</th>
<th align="center" rowspan="1" colspan="1">Aorta</th>
<th align="center" rowspan="1" colspan="1">Mesenteric</th>
</tr>
</thead>
<tbody><tr><td align="left" colspan="9" rowspan="1">(A)</td>
</tr>
<tr><td align="left" rowspan="1" colspan="1">Treatment</td>
<td align="center" rowspan="1" colspan="1">% Increase Force</td>
<td align="center" rowspan="1" colspan="1">% Increase Force</td>
<td align="center" rowspan="1" colspan="1">% Increase Force</td>
<td align="center" rowspan="1" colspan="1">% Increase Force</td>
<td align="center" rowspan="1" colspan="1">% Increase Force</td>
<td align="center" rowspan="1" colspan="1">% Increase Force</td>
<td align="center" rowspan="1" colspan="1">% Increase Force</td>
<td align="center" rowspan="1" colspan="1">% Increase Force</td>
</tr>
<tr><td align="left" rowspan="1" colspan="1">PE</td>
<td align="char" rowspan="1" colspan="1">71.8 ± 8.7</td>
<td align="char" rowspan="1" colspan="1">303.2 ± 43.5</td>
<td align="char" rowspan="1" colspan="1">95.1 ± 4.7*</td>
<td align="char" rowspan="1" colspan="1">304.1 ± 3.8</td>
<td align="char" rowspan="1" colspan="1">54.5 ± 9.8</td>
<td align="char" rowspan="1" colspan="1">289.5 ± 31.4</td>
<td align="char" rowspan="1" colspan="1">49.3 ± 9.2</td>
<td align="char" rowspan="1" colspan="1">331.5 ± 44.2</td>
</tr>
<tr><td align="left" rowspan="1" colspan="1">PE + l‐NAME</td>
<td align="char" rowspan="1" colspan="1">122.3 ± 7.0#</td>
<td align="char" rowspan="1" colspan="1">314.9 ± 43.5</td>
<td align="char" rowspan="1" colspan="1">107.9 ± 8.0#</td>
<td align="char" rowspan="1" colspan="1">363.2 ± 2.6</td>
<td align="char" rowspan="1" colspan="1">93.1 ± 6.8</td>
<td align="char" rowspan="1" colspan="1">291.8 ± 16.9</td>
<td align="char" rowspan="1" colspan="1">96.1 ± 4.5</td>
<td align="char" rowspan="1" colspan="1">354.1 ± 24.3</td>
</tr>
<tr><td align="left" rowspan="1" colspan="1">KCl</td>
<td align="char" rowspan="1" colspan="1">98.8 ± 1.0</td>
<td align="char" rowspan="1" colspan="1">132.8 ± 7.3</td>
<td align="char" rowspan="1" colspan="1">97.2 ± 4.2</td>
<td align="char" rowspan="1" colspan="1">288.4 ± 35.2*</td>
<td align="char" rowspan="1" colspan="1">94.6 ± 31.9</td>
<td align="char" rowspan="1" colspan="1">126.2 ± 10.7</td>
<td align="char" rowspan="1" colspan="1">99.2 ± 2.5</td>
<td align="char" rowspan="1" colspan="1">289.4 ± 22.5*</td>
</tr>
<tr><td align="left" rowspan="1" colspan="1">Treatment</td>
<td align="center" rowspan="1" colspan="1">% Relaxation</td>
<td align="center" rowspan="1" colspan="1">% Relaxation</td>
<td align="center" rowspan="1" colspan="1">% Relaxation</td>
<td align="center" rowspan="1" colspan="1">% Relaxation</td>
<td align="center" rowspan="1" colspan="1">% Relaxation</td>
<td align="center" rowspan="1" colspan="1">% Relaxation</td>
<td align="center" rowspan="1" colspan="1">% Relaxation</td>
<td align="center" rowspan="1" colspan="1">% Relaxation</td>
</tr>
<tr><td align="left" rowspan="1" colspan="1">ACh</td>
<td align="char" rowspan="1" colspan="1">70.2 ± 3.5</td>
<td align="char" rowspan="1" colspan="1">98.4 ± 1.5</td>
<td align="char" rowspan="1" colspan="1">56.3 ± 8.1 4<italic>ϕ</italic>
</td>
<td align="char" rowspan="1" colspan="1">98.6 ± 0.6</td>
<td align="char" rowspan="1" colspan="1">82.1 ± 2.2</td>
<td align="char" rowspan="1" colspan="1">95.5 ± 1.7</td>
<td align="char" rowspan="1" colspan="1">93.6 ± 3.67</td>
<td align="char" rowspan="1" colspan="1">100.1 ± 1.3</td>
</tr>
<tr><td align="left" rowspan="1" colspan="1">ACh + l‐NAME</td>
<td align="char" rowspan="1" colspan="1">19.2 ± 0.7*</td>
<td align="char" rowspan="1" colspan="1">87.1 ± 6.9</td>
<td align="char" rowspan="1" colspan="1">–14.2 ± 2.0</td>
<td align="char" rowspan="1" colspan="1">96.3 ± 10.3</td>
<td align="char" rowspan="1" colspan="1">–13.4 ± 4.4</td>
<td align="char" rowspan="1" colspan="1">79.9 ± 11.6</td>
<td align="char" rowspan="1" colspan="1">–14.7 ± 4.7</td>
<td align="char" rowspan="1" colspan="1">90.5 ± 2.9</td>
</tr>
<tr><td align="left" rowspan="1" colspan="1">SNP</td>
<td align="char" rowspan="1" colspan="1">93.0 ± 2.6</td>
<td align="char" rowspan="1" colspan="1">94.9 ± 3.4</td>
<td align="char" rowspan="1" colspan="1">96.3 ± 5.3</td>
<td align="char" rowspan="1" colspan="1">99.1 ± 0.84</td>
<td align="char" rowspan="1" colspan="1">89.3 ± 3.6</td>
<td align="char" rowspan="1" colspan="1">92.4 ± 1.1</td>
<td align="char" rowspan="1" colspan="1">90.4 ± 1.4</td>
<td align="char" rowspan="1" colspan="1">96.4 ± 1.7</td>
</tr>
<tr><td align="left" colspan="9" rowspan="1">(B)</td>
</tr>
<tr><td align="left" rowspan="1" colspan="1">Treatment</td>
<td align="center" rowspan="1" colspan="1">pD2, (−log EC50)</td>
<td align="center" rowspan="1" colspan="1">pD2, (−log EC50)</td>
<td align="center" rowspan="1" colspan="1">pD2, (−log EC50)</td>
<td align="center" rowspan="1" colspan="1">pD2, (−log EC50)</td>
<td align="center" rowspan="1" colspan="1">pD2, (−log EC50)</td>
<td align="center" rowspan="1" colspan="1">pD2, (−log EC50)</td>
<td align="center" rowspan="1" colspan="1">pD2, (−log EC50)</td>
<td align="center" rowspan="1" colspan="1">pD2, (−log EC50)</td>
</tr>
<tr><td align="left" rowspan="1" colspan="1">PE</td>
<td align="char" rowspan="1" colspan="1">10.39 ± 0.17</td>
<td align="char" rowspan="1" colspan="1">5.7 ± 0.1</td>
<td align="char" rowspan="1" colspan="1">9.93 ± 0.17</td>
<td align="char" rowspan="1" colspan="1">4.1 ± 0.2</td>
<td align="char" rowspan="1" colspan="1">9.98 ± 0.10</td>
<td align="char" rowspan="1" colspan="1">5.6 ± 0.1</td>
<td align="char" rowspan="1" colspan="1">9.77 ± 0.16</td>
<td align="char" rowspan="1" colspan="1">4.3 ± 0.1</td>
</tr>
<tr><td align="left" rowspan="1" colspan="1">PE + l‐NAME</td>
<td align="char" rowspan="1" colspan="1">10.54 ± 0.07</td>
<td align="char" rowspan="1" colspan="1">5.9 ± 0.2</td>
<td align="char" rowspan="1" colspan="1">10.25 ± 0.13</td>
<td align="char" rowspan="1" colspan="1">2.7 ± 0.1</td>
<td align="char" rowspan="1" colspan="1">10.41 ± 0.08</td>
<td align="char" rowspan="1" colspan="1">6.1 ± 0.1</td>
<td align="char" rowspan="1" colspan="1">10.27 ± 0.09</td>
<td align="char" rowspan="1" colspan="1">2.9 ± 0.1</td>
</tr>
<tr><td align="left" rowspan="1" colspan="1">KCl</td>
<td align="char" rowspan="1" colspan="1">1.74 ± 0.09#</td>
<td align="char" rowspan="1" colspan="1">1.7 ± 0.0</td>
<td align="char" rowspan="1" colspan="1">1.82 ± 0.12#</td>
<td align="char" rowspan="1" colspan="1">1.3 ± 0.7<italic>ϕ</italic>
</td>
<td align="char" rowspan="1" colspan="1">2.15 ± 0.12</td>
<td align="char" rowspan="1" colspan="1">1.7 ± 0.8</td>
<td align="char" rowspan="1" colspan="1">2.27 ± 0.20</td>
<td align="char" rowspan="1" colspan="1">1.4 ± 0.4<italic>ϕ</italic>
</td>
</tr>
<tr><td align="left" rowspan="1" colspan="1">ACh</td>
<td align="char" rowspan="1" colspan="1">7.25 ± 0.06</td>
<td align="char" rowspan="1" colspan="1">8.2 ± 0.3</td>
<td align="char" rowspan="1" colspan="1">7.75 ± 0.24</td>
<td align="char" rowspan="1" colspan="1">8.7 ± 0.5</td>
<td align="char" rowspan="1" colspan="1">7.09 ± 0.21</td>
<td align="char" rowspan="1" colspan="1">7.8 ± 0.2</td>
<td align="char" rowspan="1" colspan="1">7.27 ± 0.34</td>
<td align="char" rowspan="1" colspan="1">8.9 ± 0.3</td>
</tr>
<tr><td align="left" rowspan="1" colspan="1">ACh + l‐NAME</td>
<td align="char" rowspan="1" colspan="1">–</td>
<td align="char" rowspan="1" colspan="1">7.6 ± 0.2</td>
<td align="char" rowspan="1" colspan="1">–</td>
<td align="char" rowspan="1" colspan="1">6.9 ± 0.2<italic>ψ</italic>
</td>
<td align="char" rowspan="1" colspan="1">–</td>
<td align="char" rowspan="1" colspan="1">7.7 ± 0.2</td>
<td align="char" rowspan="1" colspan="1">–</td>
<td align="char" rowspan="1" colspan="1">7.1 ± 0.1<italic>ψ</italic>
</td>
</tr>
<tr><td align="left" rowspan="1" colspan="1">SNP</td>
<td align="char" rowspan="1" colspan="1">7.48 ± 0.06</td>
<td align="char" rowspan="1" colspan="1">7.4 ± 0.6</td>
<td align="char" rowspan="1" colspan="1">7.75 ± 0.12</td>
<td align="char" rowspan="1" colspan="1">7.7 ± 0.6</td>
<td align="char" rowspan="1" colspan="1">7.52 ± 0.16</td>
<td align="char" rowspan="1" colspan="1">7.8 ± 0.6</td>
<td align="char" rowspan="1" colspan="1">7.27 ± 0.09</td>
<td align="char" rowspan="1" colspan="1">7.9 ± 1.0</td>
</tr>
</tbody>
</table>
<table-wrap-foot><fn><p>l‐NAME, l‐Nitro‐Arginine Methyl Ester; SHR, spontaneously hypertensive rats; WKY, Wistar Kyoto rats.</p>
</fn>
<fn><p>*<italic>P</italic>
< 0.05 versus all other groups, <sup>#</sup>
Significant versus female rats, <italic><sup>ϕ</sup>
P</italic>
< 0.05 versus WKY rats, <sup><italic>ψ</italic>
</sup>
<italic>P</italic>
< 0.05 versus non‐treated rings.</p>
</fn>
</table-wrap-foot>
</table-wrap>
<fig id="fig01" position="float"><label>Figure 1.</label>
<caption><p>Vasoconstriction induced by PE in aorta from male and female Wistar‐Kyoto rats (WKY) and spontaneously hypertensive rats (SHR) in the absence (A) or presence (B) of l‐Nitro‐Arginine Methyl Ester (l‐NAME). Panel C illustrates the impact of l‐NAME on PE‐induced constriction within each group. Panel D illustrates the ability of NO to attenuate PE‐induced constriction, or the vasoconstrictor buffering capacity of nitric oxide synthase (NOS), which was calculated as the change in the area under the curve in the presence or absence of l‐NAME (% increase in force × mol/L). *indicates significant difference in maximal % increase in force versus all other groups, <italic>P</italic>
< 0.05. <sup>#</sup>
indicates significant differences in maximal % increase in force versus female rats, <italic>P</italic>
< 0.05. <sup><italic>ψ</italic>
</sup>
indicates significant differences in maximal increase in force versus nontreated control rings, <italic>P</italic>
< 0.05; <italic>N</italic>
= 5–7.</p>
</caption>
<graphic xlink:href="phy2-2-e12125-g1"></graphic>
</fig>
<p>In contrast, maximal constriction to PE was comparable between all groups in small mesenteric arteries (Fig. <xref ref-type="fig" rid="fig02">2</xref>
A). However, mesenteric arteries from SHR, regardless of sex, had greater sensitivity to PE‐induced constriction than mesenteric arteries from WKY (Fig. <xref ref-type="fig" rid="fig02">2</xref>
A and B; effect of sex = NS; effect of strain = <italic>P</italic>
< 0.05; interaction = NS). The individual comparisons are depicted in panel 2C. Vasoconstrictor buffering capacity of NOS in small mesenteric arteries was significantly greater in SHR than in WKY (effect of sex = NS; effect of strain = <italic>P</italic>
< 0.05; interaction = NS, Fig. <xref ref-type="fig" rid="fig02">2</xref>
D).</p>
<fig id="fig02" position="float"><label>Figure 2.</label>
<caption><p>Vasoconstriction induced by PE in small mesenteric arteries from male and female Wistar‐Kyoto rats (WKY) and spontaneously hypertensive rats (SHR) in the absence (A) or presence (B) of l‐Nitro‐Arginine Methyl Ester (l‐NAME). Panel C illustrates the impact of l‐NAME on PE‐induced constriction within each group. Panel D illustrates the ability of NO to attenuate PE‐induced constriction, or the vasoconstrictor buffering capacity of nitric oxide synthase (NOS), which was calculated as the change in the area under the curve in the presence or absence of l‐NAME (% increase in force × mol/L). <italic>ϕ</italic>
indicates significant differences in maximal % increase in force versus Wistar‐Kyoto rats (WKY), <italic>P</italic>
< 0.05. <sup><italic>ψ</italic>
</sup>
indicates significant differences in maximal increase in force versus nontreated control rings, <italic>P</italic>
< 0.05; <italic>N</italic>
= 5–6.</p>
</caption>
<graphic xlink:href="phy2-2-e12125-g2"></graphic>
</fig>
<p>Receptor‐independent vascular contraction was also assessed in response to increasing concentrations of KCl. Maximal contraction was comparable in aortic rings from all four groups (<xref ref-type="table" rid="tbl01">Table 1</xref>
and Fig. <xref ref-type="fig" rid="fig03">3</xref>
A), however, male rats showed greater sensitivity to KCl‐induced vasoconstriction than females regardless of strain. In addition, we found strain differences in KCl‐induced contraction in small mesenteric arteries (<xref ref-type="table" rid="tbl01">Table 1</xref>
and Fig. <xref ref-type="fig" rid="fig03">3</xref>
B); small mesenteric arteries from SHR displayed greater maximal KCl‐induced contraction and sensitivity compared to WKY regardless of sex.</p>
<fig id="fig03" position="float"><label>Figure 3.</label>
<caption><p>KCl‐induced constriction in aortic (A) and small mesenteric artery (B) rings from male and female Wistar‐Kyoto rats (WKY) and spontaneously hypertensive rats (SHR). <sup>#</sup>
indicates significant differences in maximal % increase in force versus female rats, <italic>P</italic>
< 0.05. <sup><italic>ϕ</italic>
</sup>
indicates significant differences in maximal % increase in force versus Wistar‐Kyoto rats (WKY), <italic>P</italic>
< 0.05. <italic>N</italic>
= 8–12.</p>
</caption>
<graphic xlink:href="phy2-2-e12125-g3"></graphic>
</fig>
</sec>
<sec><title>Relaxation</title>
<p>Endothelial‐dependent vasorelaxation was assessed by performing CCR curves to ACh in aortic rings and small mesenteric arteries from male and female SHR and WKY. Maximal relaxation to ACh was impaired in aorta from male SHR compared with other groups (effect of sex = <italic>P</italic>
< 0.05; effect of strain = NS; interaction = <italic>P</italic>
< 0.05, <xref ref-type="table" rid="tbl01">Table 1</xref>
, Fig. <xref ref-type="fig" rid="fig04">4</xref>
A). Sensitivity to ACh was comparable between aortic rings isolated from male and female SHR and WKY (<xref ref-type="table" rid="tbl01">Table 1</xref>
B). l‐NAME preincubation dramatically blunted ACh‐induced relaxation in male WKY and female rats regardless of strain, however, male WKY maintained a dilatory response to ACh in the presence of l‐NAME (Fig. <xref ref-type="fig" rid="fig04">4</xref>
B). The individual comparisons are depicted in panel 4C. Vasodilatory buffering capacity of NOS in small mesenteric arteries was significantly greater in female rats compared to male rats (effect of sex = <italic>P</italic>
< 0.05; effect of strain = <italic>P</italic>
< 0.05; interaction = NS, Fig. <xref ref-type="fig" rid="fig04">4</xref>
D).</p>
<fig id="fig04" position="float"><label>Figure 4.</label>
<caption><p>Vasorelaxation induced by ACh in aorta from male and female Wistar‐Kyoto rats (WKY) and spontaneously hypertensive rats (SHR) in the absence (A) or presence (B) of l‐Nitro‐Arginine Methyl Ester (l‐NAME). Panel C illustrates the impact of l‐NAME on ACh‐induced relaxation within each group. Panel D illustrates the ability of NO to attenuate ACh‐induced relaxation, or the contribution of NO to relaxation, which was calculated as the change in the area under the curve in the presence or absence of l‐NAME (% relaxation × mol/L). *indicates significant differences in maximal % increase in force versus all other groups, <italic>P</italic>
< 0.05. <italic><sup>ϕ</sup>
</italic>
indicates significant differences in maximal % increase in force versus WKY,<italic> P</italic>
< 0.05. <sup><italic>ψ</italic>
</sup>
indicates significant differences in maximal increase in force versus nontreated rings, <italic>P</italic>
< 0.05. <italic>N</italic>
= 5–7.</p>
</caption>
<graphic xlink:href="phy2-2-e12125-g4"></graphic>
</fig>
<p>Maximal relaxation to ACh in small mesenteric arteries was similar in all groups (<xref ref-type="table" rid="tbl01">Table 1</xref>
and Fig. <xref ref-type="fig" rid="fig05">5</xref>
A). l‐NAME preincubation attenuated ACh‐induced relaxation and sensitivity in mesenteric arteries from SHR compared to WKY (effect of sex = NS; effect of strain <italic>P</italic>
< 0.05; interaction = NS, Fig. <xref ref-type="fig" rid="fig05">5</xref>
B). The individual comparisons are depicted in panel 5C. Consequently, vasodilatory buffering capacity of NOS in small mesenteric arteries was significantly greater in SHR compared to WKY (effect of sex = NS; effect of strain = <italic>P</italic>
< 0.05; interaction = NS, Fig. <xref ref-type="fig" rid="fig05">5</xref>
D).</p>
<fig id="fig05" position="float"><label>Figure 5.</label>
<caption><p>Vasorelaxation induced by ACh in small mesenteric arteries from male and female Wistar‐Kyoto rats (WKY) and spontaneously hypertensive rats (SHR) in absence (A) or presence (B) of l‐Nitro‐Arginine Methyl Ester (l‐NAME). Panel C illustrates the impact of l‐NAME on ACh‐induced relaxation within each group. Panel D illustrates the ability of NO to attenuate ACh‐induced relaxation, or the contribution of NO to relaxation, which was calculated as the change in the area under the curve in the presence or absence of l‐NAME (% relaxation × mol/L). *indicates significant differences in maximal % increase in force versus all other groups, <italic>P</italic>
< 0.05. <sup><italic>ϕ</italic>
</sup>
indicates significant differences in maximal % increase in force versus WKY,<italic> P</italic>
< 0.05. <sup><italic>ψ</italic>
</sup>
indicates significant differences in maximal increase in force versus nontreated rings, <italic>P</italic>
< 0.05. <italic>N</italic>
= 5–7.</p>
</caption>
<graphic xlink:href="phy2-2-e12125-g5"></graphic>
</fig>
<p>To determine if sex and strain differences in response to ACh were related to vascular smooth muscle sensitivity to NO, endothelial‐independent vasorelaxation was also assessed in response to SNP. SNP‐induced relaxation was similar in aortic and small mesenteric rings from male and female SHR and WKY (<xref ref-type="table" rid="tbl01">Table 1</xref>
).</p>
</sec>
<sec><title>Total NOS activity and expression in vascular tissue</title>
<p>To begin to gain mechanistic insight into observed changes in vascular function, NOS enzymatic activity and NOS3 protein expression were assessed in aorta and mesenteric arteries of male and female SHR and WKY. Total NOS enzymatic activity was comparable in aorta from male and female SHR and WKY (Fig. <xref ref-type="fig" rid="fig06">6</xref>
A). Although NOS activity was comparable, male SHR had greater NOS3 protein expression than WKY; aortic NOS3 protein expression was similar in female SHR and WKY (Fig. <xref ref-type="fig" rid="fig06">6</xref>
B).</p>
<fig id="fig06" position="float"><label>Figure 6.</label>
<caption><p>Nitric oxide synthase (NOS) activity (A) and NOS3 protein expression (B) in isolated aorta from male and female Wistar‐Kyoto rats (WKY) and spontaneously hypertensive rats (SHR). The first lane in each representative Western blot indicates the molecular weight marker. *indicates increased protein expression versus male WKY, <italic>P</italic>
< 0.05. <italic>N</italic>
= 8–9.</p>
</caption>
<graphic xlink:href="phy2-2-e12125-g6"></graphic>
</fig>
<p>In contrast, NOS activity was significantly greater in small mesenteric arteries from WKY compared to SHR (Fig. <xref ref-type="fig" rid="fig07">7</xref>
A); NOS activity was comparable between the sexes in both strains. Consistent to what was observed in the aorta, male SHR tended to have greater NOS3 expression in small mesenteric arteries than male WKY and there were no significant difference in NOS3 expression in female SHR and WKY (Fig. <xref ref-type="fig" rid="fig07">7</xref>
B).</p>
<fig id="fig07" position="float"><label>Figure 7.</label>
<caption><p>Nitric oxide synthase (NOS) activity (A) and NOS3 protein expression (B) in the mesenteric arterial bed from male and female Wistar‐Kyoto rats (WKY) and spontaneously hypertensive rats (SHR). The first lane in each representative Western blot indicates the molecular weight marker. <sup><italic>ϕ</italic>
</sup>
indicates significant difference from M WKY,<italic> P</italic>
< 0.05. <italic>N</italic>
= 8–9.</p>
</caption>
<graphic xlink:href="phy2-2-e12125-g7"></graphic>
</fig>
</sec>
</sec>
<sec sec-type="other"><title>Discussion</title>
<p>Loss of vascular NO results in impaired vasodilation and enhanced vasoconstriction which have been suggested as a common pathway in the pathogenesis of cardiovascular disease (Giles et al. <xref rid="b16" ref-type="bibr">2012</xref>
). The current study examined the impact of both sex and blood pressure status on vascular function. At the age studied in the current manuscript, SHR have established hypertension and blood pressure is higher in the males than in the females (Sullivan et al. <xref rid="b45" ref-type="bibr">2007</xref>
; Tipton et al. <xref rid="b50" ref-type="bibr">2014</xref>
). In addition, we studied both a large conduit vessel, the aorta, and a small vessel to determine if the effects of sex and hypertension are dependent on artery type. The central findings of the current study are that male SHR exclusively exhibit altered aortic function and decreases in the NOS contribution to vascular tone, while in mesenteric arteries SHR of both sexes had enhanced vascular responses and increases in the NOS‐sensitive component. Based on the central role played by small arteries in the regulation of blood pressure, we hypothesize that SHR exhibit a compensatory increase in NOS in response to increases in blood pressure to help maintain small artery function. Additional studies are required to test this hypothesis since neither sex nor strain altered NOS enzymatic activity, and NOS3 protein expression was not increased in SHR compared to WKY. Regardless, there are sex and strain differences in aortic versus mesenteric arterial function and in the ability of NOS to modulate vascular function.</p>
<p>PE‐induced contraction and basal NOS‐mediated attenuation of contraction were comparable in female SHR and WKY, although impaired in male SHR relative to all other groups. Basal NO availability in male and female WKY and stroke‐prone SHR (SHRSP) has also been examined using l‐NAME in PE‐constricted aortic rings (McIntyre et al. <xref rid="b32" ref-type="bibr">1997</xref>
). In contrast to our findings, basal NO availability was reduced in aortas from SHRSP of both sexes compared to WKY, although females of both strains exhibited greater NO levels than males. Additional studies reported that aortic rings from male and female SHRSP exhibited greater PE‐induced constriction than rings from WKY and confirmed decreases in basal NO in both carotid arteries and aorta in SHRSP versus WKY (Dowell et al. <xref rid="b10" ref-type="bibr">1999</xref>
; Kerr et al. <xref rid="b27" ref-type="bibr">1999</xref>
). The discrepancy in these studies relative to ours may be related to SHR/SHRSP strain differences. Indeed, consistent with our study, aortic strips from male SHR exhibited greater increases in active stress than strips from female SHR and this was accompanied by greater increases in calcium influx (Crews and Khalil <xref rid="b7" ref-type="bibr">1999a</xref>
,<xref rid="b8" ref-type="bibr">b</xref>
). Aortic segments from male SHRSP also display greater contraction to calcium and increased activation of STIM1 and Orai1 relative to females, therefore, sex differences in calcium handling and signaling in aorta from female SHR may contribute to the maintenance of vascular function (Giachini et al. <xref rid="b15" ref-type="bibr">2012</xref>
) relative to males. Alternatively, 12‐week‐old male SHR have a higher blood pressure compared to females. Therefore, while the mechanism responsible for the alterations in aortic function in male SHR is unknown, higher blood pressure may also contribute.</p>
<p>Unlike aorta, small arteries play a physiologically key role in the regulation of blood flow, and ultimately contribute to blood pressure. Others have reported a greater PE‐induced contraction in small mesenteric arteries from SHR compared to WKY (Nyborg and Bevan <xref rid="b35" ref-type="bibr">1988</xref>
; Husken et al. <xref rid="b19" ref-type="bibr">1994</xref>
; Toot et al. <xref rid="b51" ref-type="bibr">2011</xref>
). In our study, we found an increased sensitivity to PE only in SHR, with similar maximal constriction in all groups. In addition, despite decreased basal NO in aorta from male SHR, SHR of both sexes exhibited an increase in NOS‐mediated attenuation of PE‐induced contraction with no effect in WKY, suggesting greater basal NO in small arteries from hypertensive animals to attenuate PE‐induced constriction. There were no sex differences in either strain in PE‐included constriction or the impact of l‐NAME, consistent with our previous publications in SHR and normotensive F344 Brown‐Norway rats (Sullivan et al. <xref rid="b44" ref-type="bibr">2004</xref>
, <xref rid="b46" ref-type="bibr">2009</xref>
).</p>
<p>To further determine if sex/strain differences in PE‐induced contraction were due to differences in the contractile machinery, we assessed KCl‐induced contraction as well. KCl‐induced maximal vasoconstriction in aortic rings was similar in male and female WKY and SHR; however, rings from male rats displayed greater KCl sensitivity than from females regardless of strain. Our data suggest that males have intrinsically increased contractile function compared to females. Consistent with our data, it has been reported that in freshly isolated cells from thoracic aorta that contraction triggered by calcium as well the basal cytosolic calcium levels are reduced in female rats regardless of the strain (Crews et al. <xref rid="b9" ref-type="bibr">1999</xref>
; Murphy and Khalil <xref rid="b34" ref-type="bibr">2000</xref>
). These data further suggest, however, that the sex/strain differences in PE‐induced constriction are not the result of innate differences in the contractile machinery, but rather reflect differences in <italic>α</italic>
‐adrenergic‐induced contraction. In contrast, SHR of both sexes exhibited greater maximal contraction to KCl compared to WKY. While maximal contraction to PE was not greater in SHR compared to WKY, there was an increased sensitivity, suggesting both alterations in calcium handling and overall contraction with the development of hypertension as well as sex and strain differences in <italic>α</italic>
‐adrenergic‐induced contraction.</p>
<p>Endothelial dysfunction is a key factor in the development of cardiovascular disease in men and women (Ballerio et al. <xref rid="b2" ref-type="bibr">2007</xref>
; Skaug et al. <xref rid="b42" ref-type="bibr">2013</xref>
) and endothelium‐dependent vascular relaxation to ACh is impaired in patients with hypertension and in various experimental models of hypertension (Ballerio et al. <xref rid="b2" ref-type="bibr">2007</xref>
; Gamboa et al. <xref rid="b13" ref-type="bibr">2007</xref>
). Consistent with male SHR exhibiting impaired aortic basal NO release, male SHR also exhibited an attenuation of ACh‐induced relaxation in the aorta. While only male SHR exhibited a decrease in maximal relaxation to ACh, aortic rings from males of both strains exhibited less NOS‐mediated relaxation compared to females. In SHR, this was due to a decrease in overall relaxation, while male WKY exhibited a degree of NOS‐independent relaxation not seen in the other groups. This was an interesting finding since ACh‐induced relaxation is almost exclusively dependent on NO (Furchgott and Zawadzki <xref rid="b12" ref-type="bibr">1980</xref>
; Kauser and Rubanyi <xref rid="b25" ref-type="bibr">1994</xref>
). Future studies will examine the mechanism responsible for the residual relaxation to ACh in the presence of l‐NAME in male WKY, yet regardless, males of both strains exhibited less agonist‐stimulated NO release. While this is consistent with numerous reports that females have greater NO levels than males (Kauser and Rubanyi <xref rid="b25" ref-type="bibr">1994</xref>
; Huang et al. <xref rid="b18" ref-type="bibr">1998</xref>
), it has previously been reported that aortic rings from SHR of both sexes have impaired ACh relaxation compared to WKY (Silva‐Antonialli et al. <xref rid="b41" ref-type="bibr">2000</xref>
). However, in that study, SHR were older and males and females were combined in a single group and not studied separately. Therefore, the decrease in function could have been “driven” by aortic segments from the males. In a separate study, aortic rings from male and female SHRSP exhibited greater sensitivity to ACh‐induced relaxation compared to WKY, although there were no apparent sex differences (Dowell et al. <xref rid="b10" ref-type="bibr">1999</xref>
). It is tempting to speculate that impaired ACh‐induced relaxation exclusively in male SHR contributes to greater increases in blood pressure or end‐organ damage compared to females. However, since the aorta is not an artery that regulates blood pressure, additional studies also included small mesenteric arteries.</p>
<p>Interestingly, ACh‐induced relaxation was comparable in all groups, although SHR exhibited greater NOS‐mediated attenuation of the dilatory response compared to WKY regardless of sex. l‐NAME had minimal impact on ACh‐induced relaxation in WKY. In small mesenteric arteries, ACh‐induced dilation is mediated by NO, dilatory prostaglandins, and EDHF (Matrougui et al. <xref rid="b31" ref-type="bibr">1997</xref>
; Takamura et al. <xref rid="b49" ref-type="bibr">1999</xref>
; Makino et al. <xref rid="b29" ref-type="bibr">2000</xref>
). Therefore, our data suggest a shift in the balance of these dilatory pathways to maintain relaxation with hypertension, such that the NOS‐mediating component is increased, potentially in response to increases in blood pressure to help limit further increases in pressure and vascular dysfunction. In contrast to our findings, relaxation to ACh in norepinephrine‐precontracted mesenteric rings was attenuated in both female and male SHR compared to WKY (Kauser and Rubanyi <xref rid="b26" ref-type="bibr">1995</xref>
), however, the lack of a sex difference in ACh‐induced relaxation is consistent with previous publications from our group (Sullivan et al. <xref rid="b44" ref-type="bibr">2004</xref>
, <xref rid="b46" ref-type="bibr">2009</xref>
). Others have shown that the relative role of NO in the endothelium‐dependent and independent relaxation was higher in female than in male SHR. However, the size of the mesenteric arteries studied was not indicated in this experiment therefore the results may be more relevant to our findings in the aorta (Kahonen et al. <xref rid="b23" ref-type="bibr">1998</xref>
).</p>
<p>To gain insight into the molecular mechanisms by which sex and strain impact vascular function, NOS activity and NOS3 expression were assessed. Despite both sex and strain differences in the relative contribution of NOS to impact vascular function, NOS enzymatic activity was comparable among all groups. Moreover, NOS3 protein expression was greater, not less, in aorta from male SHR compared to male WKY and SHR did not have greater NOS3 expression in the mesenteric arterial bed. Therefore, neither our measurements of NOS activity nor NOS3 expression explained the sex and strain differences in vascular function. It should be noted that NOS activity was measured via an enzymatic assay in the presence of excess amounts of all of the necessary cofactors and does not necessarily reflect in vivo NOS activity or NO production. In a separate study, direct in vitro measurements of NO in mesenteric arteries showed unchanged NO release, but significantly greater NO decomposition by reactive oxygen species (ROS) (Tschudi et al. <xref rid="b52" ref-type="bibr">1996</xref>
). Indeed, the attenuated basal NOS component in aorta from male SHR likely reflects differences in ROS production. NO bioavailability is determined not only by NOS activity and expression but also by scavenging of NO by superoxide (Kerr et al. <xref rid="b27" ref-type="bibr">1999</xref>
) and male SHR have greater levels of oxidative stress than female SHR (Yanes et al. <xref rid="b55" ref-type="bibr">2005</xref>
; Iliescu et al. <xref rid="b21" ref-type="bibr">2006</xref>
, <xref rid="b22" ref-type="bibr">2007</xref>
) and WKY (Kerr et al. <xref rid="b27" ref-type="bibr">1999</xref>
). Therefore, it is likely that greater levels of oxidative stress in SHR compared to WKY, particularly in males, contributes to the decrease in NOS buffering capacity in aortic tissue. The potential even exists that maintained NOS activity in SHR reflects uncoupled NOS activity, in aortic rings from male and female SHRSP treatment with l‐NAME decreases in superoxide levels (Kerr et al. <xref rid="b27" ref-type="bibr">1999</xref>
), directly implicating NOS as a source of ROS in the vasculature. It should also be noted that total NOS activity was measured, not isoform‐specific activity. In addition to NOS3, NOS1 in particular is also found in the vasculature and we have previously shown that NOS1 typically acts as a break to increases in contraction (Sullivan et al. <xref rid="b43" ref-type="bibr">2002</xref>
) in isolated mesenteric arteries. Therefore, there could be isoform‐specific changes in NOS activity, or NOS1 protein, which were not assessed in the current study.</p>
<p>It is tempting to speculate that in our study, the reduced NOS buffering capacity to PE constriction may contribute to differences in pulse pressure in a sex and strain‐dependent manner. Although not measured in the current study, it has previously been shown that male SHR show significantly higher pulse pressure than female SHR while pulse pressure in the normotensive WKY rats is comparable between males and females (Chamiot‐Clerc et al. <xref rid="b6" ref-type="bibr">2001</xref>
; Maris et al. <xref rid="b30" ref-type="bibr">2005</xref>
). Furthermore, SHR had greater pulse pressure than WKY rats when compared for each gender. This may reflect sex and strain differences in sympathetic nervous system activity, cardiac contractility, stroke volume, vasomotor tone, and/or vascular compliance.</p>
<p>In conclusion, we propose that SHR exhibit a functional increase in ACh‐induced NO production in small arteries to compensate for hypertension‐induced vascular damage. This study provides further evidence regarding sex differences in the mechanism regulating vascular tone in WKY and SHR.</p>
</sec>
<sec><title>Conflict of Interest</title>
<p>None declared.</p>
</sec>
</body>
<back><fn-group><fn fn-type="financial-disclosure"><p><bold>Funding Information</bold>
</p>
<p>This work was supported by funds from the NIH Heart, Blood and Lung Institute (HL093271 to JCS and HL HL111354 to ASL), Bethesda, Maryland.</p>
</fn>
</fn-group>
<ref-list><title>References</title>
<ref id="b1"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Anishchenko</surname>
<given-names>T. G.</given-names>
</name>
<name><surname>Igosheva</surname>
<given-names>N. B.</given-names>
</name>
</person-group>
<year>1992</year>
<article-title>Sex differences in response to stress in conscious and anesthesized rats during surgical stress</article-title>
. <source>Biull. Eksp. Biol. Med</source>
.;
<volume>113</volume>
:<fpage>26</fpage>
-<lpage>28</lpage>
.<pub-id pub-id-type="pmid">1391854</pub-id>
</mixed-citation>
</ref>
<ref id="b2"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Ballerio</surname>
<given-names>R.</given-names>
</name>
<name><surname>Gianazza</surname>
<given-names>E.</given-names>
</name>
<name><surname>Mussoni</surname>
<given-names>L.</given-names>
</name>
<name><surname>Miller</surname>
<given-names>I.</given-names>
</name>
<name><surname>Gelosa</surname>
<given-names>P.</given-names>
</name>
<name><surname>Guerrini</surname>
<given-names>U.</given-names>
</name>
</person-group>
<year>2007</year>
<article-title>Gender differences in endothelial function and inflammatory markers along the occurrence of pathological events in stroke‐prone rats</article-title>
. <source>Exp. Mol. Pathol</source>
.;
<volume>82</volume>
:<fpage>33</fpage>
-<lpage>41</lpage>
.<pub-id pub-id-type="pmid">17150211</pub-id>
</mixed-citation>
</ref>
<ref id="b3"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Baylis</surname>
<given-names>C.</given-names>
</name>
</person-group>
<year>2012</year>
<article-title>Sexual dimorphism: the aging kidney, involvement of nitric oxide deficiency, and angiotensin II overactivity</article-title>
. <source>J. Gerontol. A Biol. Sci. Med. Sci</source>
.;
<volume>67</volume>
:<fpage>1365</fpage>
-<lpage>1372</lpage>
.<pub-id pub-id-type="pmid">22960474</pub-id>
</mixed-citation>
</ref>
<ref id="b4"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Baylis</surname>
<given-names>C.</given-names>
</name>
<name><surname>Mitruka</surname>
<given-names>B.</given-names>
</name>
<name><surname>Deng</surname>
<given-names>A.</given-names>
</name>
</person-group>
<year>1992</year>
<article-title>Chronic blockade of nitric oxide synthesis in the rat produces systemic hypertension and glomerular damage</article-title>
. <source>J. Clin. Invest</source>
.;
<volume>90</volume>
:<fpage>278</fpage>
-<lpage>281</lpage>
.<pub-id pub-id-type="pmid">1634615</pub-id>
</mixed-citation>
</ref>
<ref id="b5"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Brinson</surname>
<given-names>K. N.</given-names>
</name>
<name><surname>Elmarakby</surname>
<given-names>A. A.</given-names>
</name>
<name><surname>Tipton</surname>
<given-names>A. J.</given-names>
</name>
<name><surname>Crislip</surname>
<given-names>G. R.</given-names>
</name>
<name><surname>Yamamoto</surname>
<given-names>T.</given-names>
</name>
<name><surname>Baban</surname>
<given-names>B.</given-names>
</name>
</person-group>
<year>2013</year>
<article-title>Female SHR have greater blood pressure sensitivity and renal T cell infiltration following chronic NOS inhibition than males</article-title>
. <source>Am. J. Physiol. Regul. Integr. Comp. Physiol</source>
.;
<volume>305</volume>
:<fpage>R701</fpage>
-<lpage>R710</lpage>
.<pub-id pub-id-type="pmid">23883679</pub-id>
</mixed-citation>
</ref>
<ref id="b6"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Chamiot‐Clerc</surname>
<given-names>P.</given-names>
</name>
<name><surname>Renaud</surname>
<given-names>J. F.</given-names>
</name>
<name><surname>Safar</surname>
<given-names>M. E.</given-names>
</name>
</person-group>
<year>2001</year>
<article-title>Pulse pressure, aortic reactivity, and endothelium dysfunction in old hypertensive rats</article-title>
. <source>Hypertension</source>
;
<volume>37</volume>
:<fpage>313</fpage>
-<lpage>321</lpage>
.<pub-id pub-id-type="pmid">11230291</pub-id>
</mixed-citation>
</ref>
<ref id="b7"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Crews</surname>
<given-names>J. K.</given-names>
</name>
<name><surname>Khalil</surname>
<given-names>R. A.</given-names>
</name>
</person-group>
<year>1999a</year>
<article-title>Antagonistic effects of 17 beta‐estradiol, progesterone, and testosterone on Ca<sup>2+</sup>
entry mechanisms of coronary vasoconstriction</article-title>
. <source>Arterioscler. Thromb. Vasc. Biol</source>
.;
<volume>19</volume>
:<fpage>1034</fpage>
-<lpage>1040</lpage>
.<pub-id pub-id-type="pmid">10195933</pub-id>
</mixed-citation>
</ref>
<ref id="b8"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Crews</surname>
<given-names>J. K.</given-names>
</name>
<name><surname>Khalil</surname>
<given-names>R. A.</given-names>
</name>
</person-group>
<year>1999b</year>
<article-title>Gender‐specific inhibition of Ca<sup>2+</sup>
entry mechanisms of arterial vasoconstriction by sex hormones</article-title>
. <source>Clin. Exp. Pharmacol. Physiol</source>
.;
<volume>26</volume>
:<fpage>707</fpage>
-<lpage>715</lpage>
.<pub-id pub-id-type="pmid">10499160</pub-id>
</mixed-citation>
</ref>
<ref id="b9"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Crews</surname>
<given-names>J. K.</given-names>
</name>
<name><surname>Murphy</surname>
<given-names>J. G.</given-names>
</name>
<name><surname>Khalil</surname>
<given-names>R. A.</given-names>
</name>
</person-group>
<year>1999</year>
<article-title>Gender differences in Ca(2+) entry mechanisms of vasoconstriction in Wistar‐Kyoto and spontaneously hypertensive rats</article-title>
. <source>Hypertension</source>
;
<volume>34</volume>
4 Pt 2:<fpage>931</fpage>
-<lpage>936</lpage>
.<pub-id pub-id-type="pmid">10523387</pub-id>
</mixed-citation>
</ref>
<ref id="b10"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Dowell</surname>
<given-names>F. J.</given-names>
</name>
<name><surname>Martin</surname>
<given-names>W.</given-names>
</name>
<name><surname>Dominiczak</surname>
<given-names>A. F.</given-names>
</name>
<name><surname>Hamilton</surname>
<given-names>C. A.</given-names>
</name>
</person-group>
<year>1999</year>
<article-title>Decreased basal despite enhanced agonist‐stimulated effects of nitric oxide in 12‐week‐old stroke‐prone spontaneously hypertensive rat</article-title>
. <source>Eur. J. Pharmacol</source>
.;
<volume>379</volume>
:<fpage>175</fpage>
-<lpage>182</lpage>
.<pub-id pub-id-type="pmid">10497904</pub-id>
</mixed-citation>
</ref>
<ref id="b11"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Forte</surname>
<given-names>P.</given-names>
</name>
<name><surname>Kneale</surname>
<given-names>B. J.</given-names>
</name>
<name><surname>Milne</surname>
<given-names>E.</given-names>
</name>
<name><surname>Chowienczyk</surname>
<given-names>P. J.</given-names>
</name>
<name><surname>Johnston</surname>
<given-names>A.</given-names>
</name>
<name><surname>Benjamin</surname>
<given-names>N.</given-names>
</name>
</person-group>
<year>1998</year>
<article-title>Evidence for a difference in nitric oxide biosynthesis between healthy women and men</article-title>
. <source>Hypertension</source>
;
<volume>32</volume>
:<fpage>730</fpage>
-<lpage>734</lpage>
.<pub-id pub-id-type="pmid">9774371</pub-id>
</mixed-citation>
</ref>
<ref id="b12"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Furchgott</surname>
<given-names>R. F.</given-names>
</name>
<name><surname>Zawadzki</surname>
<given-names>J. V.</given-names>
</name>
</person-group>
<year>1980</year>
<article-title>The obligatory role of endothelial cells in the relaxation of arterial smooth muscle by acetylcholine</article-title>
. <source>Nature</source>
;
<volume>288</volume>
:<fpage>373</fpage>
-<lpage>376</lpage>
.<pub-id pub-id-type="pmid">6253831</pub-id>
</mixed-citation>
</ref>
<ref id="b13"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Gamboa</surname>
<given-names>A.</given-names>
</name>
<name><surname>Shibao</surname>
<given-names>C.</given-names>
</name>
<name><surname>Diedrich</surname>
<given-names>A.</given-names>
</name>
<name><surname>Choi</surname>
<given-names>L.</given-names>
</name>
<name><surname>Pohar</surname>
<given-names>B.</given-names>
</name>
<name><surname>Jordan</surname>
<given-names>J.</given-names>
</name>
</person-group>
<year>2007</year>
<article-title>Contribution of endothelial nitric oxide to blood pressure in humans</article-title>
. <source>Hypertension</source>
;
<volume>49</volume>
:<fpage>170</fpage>
-<lpage>177</lpage>
.<pub-id pub-id-type="pmid">17130304</pub-id>
</mixed-citation>
</ref>
<ref id="b14"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Gerhard</surname>
<given-names>M.</given-names>
</name>
<name><surname>Ganz</surname>
<given-names>P.</given-names>
</name>
</person-group>
<year>1995</year>
<article-title>How do we explain the clinical benefits of estrogen? From bedside to bench</article-title>
. <source>Circulation</source>
;
<volume>92</volume>
:<fpage>5</fpage>
-<lpage>8</lpage>
.<pub-id pub-id-type="pmid">7788916</pub-id>
</mixed-citation>
</ref>
<ref id="b15"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Giachini</surname>
<given-names>F. R.</given-names>
</name>
<name><surname>Lima</surname>
<given-names>V. V.</given-names>
</name>
<name><surname>Filgueira</surname>
<given-names>F. P.</given-names>
</name>
<name><surname>Dorrance</surname>
<given-names>A. M.</given-names>
</name>
<name><surname>Carvalho</surname>
<given-names>M. H.</given-names>
</name>
<name><surname>Fortes</surname>
<given-names>Z. B.</given-names>
</name>
</person-group>
<year>2012</year>
<article-title>STIM1/Orai1 contributes to sex differences in vascular responses to calcium in spontaneously hypertensive rats</article-title>
. <source>Clin. Sci. (Lond.)</source>
;
<volume>122</volume>
:<fpage>215</fpage>
-<lpage>226</lpage>
.<pub-id pub-id-type="pmid">21966957</pub-id>
</mixed-citation>
</ref>
<ref id="b16"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Giles</surname>
<given-names>T. D.</given-names>
</name>
<name><surname>Sander</surname>
<given-names>G. E.</given-names>
</name>
<name><surname>Nossaman</surname>
<given-names>B. D.</given-names>
</name>
<name><surname>Kadowitz</surname>
<given-names>P. J.</given-names>
</name>
</person-group>
<year>2012</year>
<article-title>Impaired vasodilation in the pathogenesis of hypertension: focus on nitric oxide, endothelial‐derived hyperpolarizing factors, and prostaglandins</article-title>
. <source>J. Clin. Hypertens. (Greenwich)</source>
;
<volume>14</volume>
:<fpage>198</fpage>
-<lpage>205</lpage>
.<pub-id pub-id-type="pmid">22458740</pub-id>
</mixed-citation>
</ref>
<ref id="b17"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Glushkovskaya‐Semyachkina</surname>
<given-names>O. V.</given-names>
</name>
<name><surname>Anishchenko</surname>
<given-names>T. G.</given-names>
</name>
<name><surname>Sindyakova</surname>
<given-names>T. A.</given-names>
</name>
<name><surname>Leksina</surname>
<given-names>O. V.</given-names>
</name>
<name><surname>Berdnikova</surname>
<given-names>V. A.</given-names>
</name>
</person-group>
<year>2006</year>
<article-title>Sex‐related differences in nitric oxide content in healthy and hypertensive rats at rest and under stress conditions</article-title>
. <source>Bull. Exp. Biol. Med</source>
.;
<volume>142</volume>
:<fpage>9</fpage>
-<lpage>11</lpage>
.<pub-id pub-id-type="pmid">17369889</pub-id>
</mixed-citation>
</ref>
<ref id="b18"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Huang</surname>
<given-names>A.</given-names>
</name>
<name><surname>Sun</surname>
<given-names>D.</given-names>
</name>
<name><surname>Kaley</surname>
<given-names>G.</given-names>
</name>
<name><surname>Koller</surname>
<given-names>A.</given-names>
</name>
</person-group>
<year>1998</year>
<article-title>Estrogen preserves regulation of shear stress by nitric oxide in arterioles of female hypertensive rats</article-title>
. <source>Hypertension</source>
;
<volume>31</volume>
1 Pt 2:<fpage>309</fpage>
-<lpage>314</lpage>
.<pub-id pub-id-type="pmid">9453321</pub-id>
</mixed-citation>
</ref>
<ref id="b19"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Husken</surname>
<given-names>B. C.</given-names>
</name>
<name><surname>Mertens</surname>
<given-names>M. J.</given-names>
</name>
<name><surname>Pfaffendorf</surname>
<given-names>M.</given-names>
</name>
<name><surname>Van Zwieten</surname>
<given-names>P. A.</given-names>
</name>
</person-group>
<year>1994</year>
<article-title>The influence of coarctation hypertension on the pharmacodynamic behaviour of rat isolated conduit vessels</article-title>
. <source>Blood Press</source>
.;
<volume>3</volume>
:<fpage>255</fpage>
-<lpage>259</lpage>
.<pub-id pub-id-type="pmid">7994451</pub-id>
</mixed-citation>
</ref>
<ref id="b21"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Iliescu</surname>
<given-names>R.</given-names>
</name>
<name><surname>Yanes</surname>
<given-names>L. L.</given-names>
</name>
<name><surname>Bell</surname>
<given-names>W.</given-names>
</name>
<name><surname>Dwyer</surname>
<given-names>T.</given-names>
</name>
<name><surname>Baltatu</surname>
<given-names>O. C.</given-names>
</name>
<name><surname>Reckelhoff</surname>
<given-names>J. F.</given-names>
</name>
</person-group>
<year>2006</year>
<article-title>Role of the renal nerves in blood pressure in male and female SHR</article-title>
. <source>Am. J. Physiol. Regul. Integr. Comp. Physiol</source>
.;
<volume>290</volume>
:<fpage>R341</fpage>
-<lpage>R344</lpage>
.<pub-id pub-id-type="pmid">16166211</pub-id>
</mixed-citation>
</ref>
<ref id="b22"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Iliescu</surname>
<given-names>R.</given-names>
</name>
<name><surname>Cucchiarelli</surname>
<given-names>V. E.</given-names>
</name>
<name><surname>Yanes</surname>
<given-names>L. L.</given-names>
</name>
<name><surname>Iles</surname>
<given-names>J. W.</given-names>
</name>
<name><surname>Reckelhoff</surname>
<given-names>J. F.</given-names>
</name>
</person-group>
<year>2007</year>
<article-title>Impact of androgen‐induced oxidative stress on hypertension in male SHR</article-title>
. <source>Am. J. Physiol. Regul. Integr. Comp. Physiol</source>
.;
<volume>292</volume>
:<fpage>R731</fpage>
-<lpage>R735</lpage>
.<pub-id pub-id-type="pmid">16971373</pub-id>
</mixed-citation>
</ref>
<ref id="b23"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Kahonen</surname>
<given-names>M.</given-names>
</name>
<name><surname>Tolvanen</surname>
<given-names>J. P.</given-names>
</name>
<name><surname>Sallinen</surname>
<given-names>K.</given-names>
</name>
<name><surname>Wu</surname>
<given-names>.</given-names>
</name>
<name><surname>Porsti</surname>
<given-names>I.</given-names>
</name>
</person-group>
<year>1998</year>
<article-title>Influence of gender on control of arterial tone in experimental hypertension</article-title>
. <source>Am. J. Physiol</source>
.;
<volume>275</volume>
1 Pt 2:<fpage>H15</fpage>
-<lpage>H22</lpage>
.<pub-id pub-id-type="pmid">9688891</pub-id>
</mixed-citation>
</ref>
<ref id="b24"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Kang</surname>
<given-names>K. T.</given-names>
</name>
<name><surname>Sullivan</surname>
<given-names>J. C.</given-names>
</name>
<name><surname>Sasser</surname>
<given-names>J. M.</given-names>
</name>
<name><surname>Imig</surname>
<given-names>J. D.</given-names>
</name>
<name><surname>Pollock</surname>
<given-names>J. S.</given-names>
</name>
</person-group>
<year>2007</year>
<article-title>Novel nitric oxide synthase–dependent mechanism of vasorelaxation in small arteries from hypertensive rats</article-title>
. <source>Hypertension</source>
;
<volume>49</volume>
:<fpage>893</fpage>
-<lpage>901</lpage>
.<pub-id pub-id-type="pmid">17309950</pub-id>
</mixed-citation>
</ref>
<ref id="b25"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Kauser</surname>
<given-names>K.</given-names>
</name>
<name><surname>Rubanyi</surname>
<given-names>G. M.</given-names>
</name>
</person-group>
<year>1994</year>
<article-title>Gender difference in bioassayable endothelium‐derived nitric oxide from isolated rat aortae</article-title>
. <source>Am. J. Physiol</source>
.;
<volume>267</volume>
6 Pt 2:<fpage>H2311</fpage>
-<lpage>H2317</lpage>
.<pub-id pub-id-type="pmid">7810731</pub-id>
</mixed-citation>
</ref>
<ref id="b26"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Kauser</surname>
<given-names>K.</given-names>
</name>
<name><surname>Rubanyi</surname>
<given-names>G. M.</given-names>
</name>
</person-group>
<year>1995</year>
<article-title>Gender difference in endothelial dysfunction in the aorta of spontaneously hypertensive rats</article-title>
. <source>Hypertension</source>
;
<volume>25</volume>
4 Pt 1:<fpage>517</fpage>
-<lpage>523</lpage>
.<pub-id pub-id-type="pmid">7721392</pub-id>
</mixed-citation>
</ref>
<ref id="b27"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Kerr</surname>
<given-names>S.</given-names>
</name>
<name><surname>Brosnan</surname>
<given-names>M. J.</given-names>
</name>
<name><surname>McIntyre</surname>
<given-names>M.</given-names>
</name>
<name><surname>Reid</surname>
<given-names>J. L.</given-names>
</name>
<name><surname>Dominiczak</surname>
<given-names>A. F.</given-names>
</name>
<name><surname>Hamilton</surname>
<given-names>C. A.</given-names>
</name>
</person-group>
<year>1999</year>
<article-title>Superoxide anion production is increased in a model of genetic hypertension: role of the endothelium</article-title>
. <source>Hypertension</source>
;
<volume>33</volume>
:<fpage>1353</fpage>
-<lpage>1358</lpage>
.<pub-id pub-id-type="pmid">10373215</pub-id>
</mixed-citation>
</ref>
<ref id="b28"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Loria</surname>
<given-names>A. S.</given-names>
</name>
<name><surname>Kang</surname>
<given-names>K. T.</given-names>
</name>
<name><surname>Pollock</surname>
<given-names>D. M.</given-names>
</name>
<name><surname>Pollock</surname>
<given-names>J. S.</given-names>
</name>
</person-group>
<year>2011</year>
<article-title>Early life stress enhances angiotensin II‐mediated vasoconstriction by reduced endothelial nitric oxide buffering capacity</article-title>
. <source>Hypertension</source>
;
<volume>58</volume>
:<fpage>619</fpage>
-<lpage>626</lpage>
.<pub-id pub-id-type="pmid">21876076</pub-id>
</mixed-citation>
</ref>
<ref id="b29"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Makino</surname>
<given-names>A.</given-names>
</name>
<name><surname>Ohuchi</surname>
<given-names>K.</given-names>
</name>
<name><surname>Kamata</surname>
<given-names>K.</given-names>
</name>
</person-group>
<year>2000</year>
<article-title>Mechanisms underlying the attenuation of endothelium‐dependent vasodilatation in the mesenteric arterial bed of the streptozotocin‐induced diabetic rat</article-title>
. <source>Br. J. Pharmacol</source>
.;
<volume>130</volume>
:<fpage>549</fpage>
-<lpage>556</lpage>
.<pub-id pub-id-type="pmid">10821782</pub-id>
</mixed-citation>
</ref>
<ref id="b30"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Maris</surname>
<given-names>M. E.</given-names>
</name>
<name><surname>Melchert</surname>
<given-names>R. B.</given-names>
</name>
<name><surname>Joseph</surname>
<given-names>J.</given-names>
</name>
<name><surname>Kennedy</surname>
<given-names>R. H.</given-names>
</name>
</person-group>
<year>2005</year>
<article-title>Gender differences in blood pressure and heart rate in spontaneously hypertensive and Wistar‐Kyoto rats</article-title>
. <source>Clin. Exp. Pharmacol. Physiol</source>
.;
<volume>32</volume>
:<fpage>35</fpage>
-<lpage>39</lpage>
.<pub-id pub-id-type="pmid">15730432</pub-id>
</mixed-citation>
</ref>
<ref id="b31"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Matrougui</surname>
<given-names>K.</given-names>
</name>
<name><surname>Maclouf</surname>
<given-names>J.</given-names>
</name>
<name><surname>Levy</surname>
<given-names>B. I.</given-names>
</name>
<name><surname>Henrion</surname>
<given-names>D.</given-names>
</name>
</person-group>
<year>1997</year>
<article-title>Impaired nitric oxide‐ and prostaglandin‐mediated responses to flow in resistance arteries of hypertensive rats</article-title>
. <source>Hypertension</source>
;
<volume>30</volume>
:<fpage>942</fpage>
-<lpage>947</lpage>
.<pub-id pub-id-type="pmid">9336397</pub-id>
</mixed-citation>
</ref>
<ref id="b32"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>McIntyre</surname>
<given-names>M.</given-names>
</name>
<name><surname>Hamilton</surname>
<given-names>C. A.</given-names>
</name>
<name><surname>Rees</surname>
<given-names>D. D.</given-names>
</name>
<name><surname>Reid</surname>
<given-names>J. L.</given-names>
</name>
<name><surname>Dominiczak</surname>
<given-names>A. F.</given-names>
</name>
</person-group>
<year>1997</year>
<article-title>Sex differences in the abundance of endothelial nitric oxide in a model of genetic hypertension</article-title>
. <source>Hypertension</source>
;
<volume>30</volume>
:<fpage>1517</fpage>
-<lpage>1524</lpage>
.<pub-id pub-id-type="pmid">9403576</pub-id>
</mixed-citation>
</ref>
<ref id="b33"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Miller</surname>
<given-names>V. M.</given-names>
</name>
</person-group>
<year>2010</year>
<article-title>Sex‐based differences in vascular function</article-title>
. <source>Womens Health (Lond. Engl.)</source>
;
<volume>6</volume>
:<fpage>737</fpage>
-<lpage>752</lpage>
.<pub-id pub-id-type="pmid">20887171</pub-id>
</mixed-citation>
</ref>
<ref id="b34"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Murphy</surname>
<given-names>J. G.</given-names>
</name>
<name><surname>Khalil</surname>
<given-names>R. A.</given-names>
</name>
</person-group>
<year>2000</year>
<article-title>Gender‐specific reduction in contractility and [Ca(2+)](i) in vascular smooth muscle cells of female rat</article-title>
. <source>Am. J. Physiol. Cell Physiol</source>
.;
<volume>278</volume>
:<fpage>C834</fpage>
-<lpage>C844</lpage>
.<pub-id pub-id-type="pmid">10751331</pub-id>
</mixed-citation>
</ref>
<ref id="b35"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Nyborg</surname>
<given-names>N. C.</given-names>
</name>
<name><surname>Bevan</surname>
<given-names>J. A.</given-names>
</name>
</person-group>
<year>1988</year>
<article-title>Increased alpha‐adrenergic receptor affinity in resistance vessels from hypertensive rats</article-title>
. <source>Hypertension</source>
;
<volume>11</volume>
6 Pt 2:<fpage>635</fpage>
-<lpage>638</lpage>
.<pub-id pub-id-type="pmid">2839417</pub-id>
</mixed-citation>
</ref>
<ref id="b36"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Orshal</surname>
<given-names>J. M.</given-names>
</name>
<name><surname>Khalil</surname>
<given-names>R. A.</given-names>
</name>
</person-group>
<year>2004</year>
<article-title>Gender, sex hormones, and vascular tone</article-title>
. <source>Am. J. Physiol. Regul. Integr. Comp. Physiol</source>
.;
<volume>286</volume>
:<fpage>R233</fpage>
-<lpage>R249</lpage>
.<pub-id pub-id-type="pmid">14707008</pub-id>
</mixed-citation>
</ref>
<ref id="b37"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Piech</surname>
<given-names>A.</given-names>
</name>
<name><surname>Dessy</surname>
<given-names>C.</given-names>
</name>
<name><surname>Havaux</surname>
<given-names>X.</given-names>
</name>
<name><surname>Feron</surname>
<given-names>O.</given-names>
</name>
<name><surname>Balligand</surname>
<given-names>J. L.</given-names>
</name>
</person-group>
<year>2003</year>
<article-title>Differential regulation of nitric oxide synthases and their allosteric regulators in heart and vessels of hypertensive rats</article-title>
. <source>Cardiovasc. Res</source>
.;
<volume>57</volume>
:<fpage>456</fpage>
-<lpage>467</lpage>
.<pub-id pub-id-type="pmid">12566118</pub-id>
</mixed-citation>
</ref>
<ref id="b38"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Ribeiro</surname>
<given-names>M. O.</given-names>
</name>
<name><surname>Antunes</surname>
<given-names>E.</given-names>
</name>
<name><surname>de Nucci</surname>
<given-names>G.</given-names>
</name>
<name><surname>Lovisolo</surname>
<given-names>S. M.</given-names>
</name>
<name><surname>Zatz</surname>
<given-names>R.</given-names>
</name>
</person-group>
<year>1992</year>
<article-title>Chronic inhibition of nitric oxide synthesis. A new model of arterial hypertension</article-title>
. <source>Hypertension</source>
;
<volume>20</volume>
:<fpage>298</fpage>
-<lpage>303</lpage>
.<pub-id pub-id-type="pmid">1516948</pub-id>
</mixed-citation>
</ref>
<ref id="b39"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Sader</surname>
<given-names>M. A.</given-names>
</name>
<name><surname>Celermajer</surname>
<given-names>D. S.</given-names>
</name>
</person-group>
<year>2002</year>
<article-title>Endothelial function, vascular reactivity and gender differences in the cardiovascular system</article-title>
. <source>Cardiovasc. Res</source>
.;
<volume>53</volume>
:<fpage>597</fpage>
-<lpage>604</lpage>
.<pub-id pub-id-type="pmid">11861030</pub-id>
</mixed-citation>
</ref>
<ref id="b40"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Sasser</surname>
<given-names>J. M.</given-names>
</name>
<name><surname>Sullivan</surname>
<given-names>J. C.</given-names>
</name>
<name><surname>Elmarakby</surname>
<given-names>A. A.</given-names>
</name>
<name><surname>Kemp</surname>
<given-names>B. E.</given-names>
</name>
<name><surname>Pollock</surname>
<given-names>D. M.</given-names>
</name>
<name><surname>Pollock</surname>
<given-names>J. S.</given-names>
</name>
</person-group>
<year>2004</year>
<article-title>Reduced NOS3 phosphorylation mediates reduced NO/cGMP signaling in mesenteric arteries of deoxycorticosterone acetate‐salt hypertensive rats</article-title>
. <source>Hypertension</source>
;
<volume>43</volume>
:<fpage>1080</fpage>
-<lpage>1085</lpage>
.<pub-id pub-id-type="pmid">14993198</pub-id>
</mixed-citation>
</ref>
<ref id="b41"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Silva‐Antonialli</surname>
<given-names>M. M.</given-names>
</name>
<name><surname>Fortes</surname>
<given-names>Z. B.</given-names>
</name>
<name><surname>Carvalho</surname>
<given-names>M. H.</given-names>
</name>
<name><surname>Scivoletto</surname>
<given-names>R.</given-names>
</name>
<name><surname>Nigro</surname>
<given-names>D.</given-names>
</name>
</person-group>
<year>2000</year>
<article-title>Sexual dimorphism in the response of thoracic aorta from SHRs to losartan</article-title>
. <source>Gen. Pharmacol</source>
.;
<volume>34</volume>
:<fpage>329</fpage>
-<lpage>335</lpage>
.<pub-id pub-id-type="pmid">11368888</pub-id>
</mixed-citation>
</ref>
<ref id="b42"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Skaug</surname>
<given-names>E. A.</given-names>
</name>
<name><surname>Aspenes</surname>
<given-names>S. T.</given-names>
</name>
<name><surname>Oldervoll</surname>
<given-names>L.</given-names>
</name>
<name><surname>Morkedal</surname>
<given-names>B.</given-names>
</name>
<name><surname>Vatten</surname>
<given-names>L.</given-names>
</name>
<name><surname>Wisloff</surname>
<given-names>U.</given-names>
</name>
</person-group>
<year>2013</year>
<article-title>Age and gender differences of endothelial function in 4739 healthy adults: the HUNT3 Fitness Study</article-title>
. <source>Eur. J. Prev. Cardiol</source>
.;
<volume>20</volume>
:<fpage>531</fpage>
-<lpage>540</lpage>
.<pub-id pub-id-type="pmid">22456692</pub-id>
</mixed-citation>
</ref>
<ref id="b43"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Sullivan</surname>
<given-names>J. C.</given-names>
</name>
<name><surname>Pollock</surname>
<given-names>D. M.</given-names>
</name>
<name><surname>Pollock</surname>
<given-names>J. S.</given-names>
</name>
</person-group>
<year>2002</year>
<article-title>Altered nitric oxide synthase 3 distribution in mesenteric arteries of hypertensive rats</article-title>
. <source>Hypertension</source>
;
<volume>39</volume>
2 Pt 2:<fpage>597</fpage>
-<lpage>602</lpage>
.<pub-id pub-id-type="pmid">11882615</pub-id>
</mixed-citation>
</ref>
<ref id="b44"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Sullivan</surname>
<given-names>J. C.</given-names>
</name>
<name><surname>Loomis</surname>
<given-names>E. D.</given-names>
</name>
<name><surname>Collins</surname>
<given-names>M.</given-names>
</name>
<name><surname>Imig</surname>
<given-names>J. D.</given-names>
</name>
<name><surname>Inscho</surname>
<given-names>E. W.</given-names>
</name>
<name><surname>Pollock</surname>
<given-names>J. S.</given-names>
</name>
</person-group>
<year>2004</year>
<article-title>Age‐related alterations in NOS and oxidative stress in mesenteric arteries from male and female rats</article-title>
. <source>J. Appl. Physiol</source>
.;
<volume>97</volume>
:<fpage>1268</fpage>
-<lpage>1274</lpage>
.<pub-id pub-id-type="pmid">15169747</pub-id>
</mixed-citation>
</ref>
<ref id="b45"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Sullivan</surname>
<given-names>J. C.</given-names>
</name>
<name><surname>Semprun‐Prieto</surname>
<given-names>L.</given-names>
</name>
<name><surname>Boesen</surname>
<given-names>E. I.</given-names>
</name>
<name><surname>Pollock</surname>
<given-names>D. M.</given-names>
</name>
<name><surname>Pollock</surname>
<given-names>J. S.</given-names>
</name>
</person-group>
<year>2007</year>
<article-title>Sex and sex hormones influence the development of albuminuria and renal macrophage infiltration in spontaneously hypertensive rats</article-title>
. <source>Am. J. Physiol. Regul. Integr. Comp. Physiol</source>
.;
<volume>293</volume>
:<fpage>R1573</fpage>
-<lpage>R1579</lpage>
.<pub-id pub-id-type="pmid">17699561</pub-id>
</mixed-citation>
</ref>
<ref id="b46"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Sullivan</surname>
<given-names>J. C.</given-names>
</name>
<name><surname>Pardieck</surname>
<given-names>J. L.</given-names>
</name>
<name><surname>Doran</surname>
<given-names>D.</given-names>
</name>
<name><surname>Zhang</surname>
<given-names>Y.</given-names>
</name>
<name><surname>She</surname>
<given-names>J. X.</given-names>
</name>
<name><surname>Pollock</surname>
<given-names>J. S.</given-names>
</name>
</person-group>
<year>2009</year>
<article-title>Greater fractalkine expression in mesenteric arteries of female spontaneously hypertensive rats compared with males</article-title>
. <source>Am. J. Physiol. Heart. Circ. Physiol</source>
.;
<volume>296</volume>
:<fpage>H1080</fpage>
-<lpage>H1088</lpage>
.<pub-id pub-id-type="pmid">19201996</pub-id>
</mixed-citation>
</ref>
<ref id="b47"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Sullivan</surname>
<given-names>J. C.</given-names>
</name>
<name><surname>Pardieck</surname>
<given-names>J. L.</given-names>
</name>
<name><surname>Hyndman</surname>
<given-names>K. A.</given-names>
</name>
<name><surname>Pollock</surname>
<given-names>J. S.</given-names>
</name>
</person-group>
<year>2010</year>
<article-title>Renal NOS activity, expression, and localization in male and female spontaneously hypertensive rats</article-title>
. <source>Am. J. Physiol. Regul. Integr. Comp. Physiol</source>
.;
<volume>298</volume>
:<fpage>R61</fpage>
-<lpage>R69</lpage>
.<pub-id pub-id-type="pmid">19889864</pub-id>
</mixed-citation>
</ref>
<ref id="b48"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Sun</surname>
<given-names>T.</given-names>
</name>
<name><surname>Wang</surname>
<given-names>J.</given-names>
</name>
<name><surname>Huang</surname>
<given-names>L. H.</given-names>
</name>
<name><surname>Cao</surname>
<given-names>Y. X.</given-names>
</name>
</person-group>
<year>2013</year>
<article-title>Antihypertensive effect of formononetin through regulating the expressions of eNOS, 5‐HT2A/1B receptors and alpha1‐adrenoceptors in spontaneously rat arteries</article-title>
. <source>Eur. J. Pharmacol</source>
.;
<volume>699</volume>
:<fpage>241</fpage>
-<lpage>249</lpage>
.<pub-id pub-id-type="pmid">23123056</pub-id>
</mixed-citation>
</ref>
<ref id="b49"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Takamura</surname>
<given-names>Y.</given-names>
</name>
<name><surname>Shimokawa</surname>
<given-names>H.</given-names>
</name>
<name><surname>Zhao</surname>
<given-names>H.</given-names>
</name>
<name><surname>Igarashi</surname>
<given-names>H.</given-names>
</name>
<name><surname>Egashira</surname>
<given-names>K.</given-names>
</name>
<name><surname>Takeshita</surname>
<given-names>A.</given-names>
</name>
</person-group>
<year>1999</year>
<article-title>Important role of endothelium‐derived hyperpolarizing factor in shear stress–induced endothelium‐dependent relaxations in the rat mesenteric artery</article-title>
. <source>J. Cardiovasc. Pharmacol</source>
.;
<volume>34</volume>
:<fpage>381</fpage>
-<lpage>387</lpage>
.<pub-id pub-id-type="pmid">10470996</pub-id>
</mixed-citation>
</ref>
<ref id="b50"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Tipton</surname>
<given-names>A. J.</given-names>
</name>
<name><surname>Baban</surname>
<given-names>B.</given-names>
</name>
<name><surname>Sullivan</surname>
<given-names>J. C.</given-names>
</name>
</person-group>
<year>2014</year>
<article-title>Female spontaneously hypertensive rats have a compensatory increase in renal regulatory T cells in response to elevations in blood pressure</article-title>
. <source>Hypertension</source>
</mixed-citation>
</ref>
<ref id="b51"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Toot</surname>
<given-names>J. D.</given-names>
</name>
<name><surname>Reho</surname>
<given-names>J. J.</given-names>
</name>
<name><surname>Novak</surname>
<given-names>J.</given-names>
</name>
<name><surname>Dunphy</surname>
<given-names>G.</given-names>
</name>
<name><surname>Ely</surname>
<given-names>D. L.</given-names>
</name>
<name><surname>Ramirez</surname>
<given-names>R. J.</given-names>
</name>
</person-group>
<year>2011</year>
<article-title>Colony social stress differentially alters blood pressure and resistance‐sized mesenteric artery reactivity in SHR/y and WKY male rats</article-title>
. <source>Stress</source>
;
<volume>14</volume>
:<fpage>33</fpage>
-<lpage>41</lpage>
.<pub-id pub-id-type="pmid">20666653</pub-id>
</mixed-citation>
</ref>
<ref id="b52"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Tschudi</surname>
<given-names>M. R.</given-names>
</name>
<name><surname>Mesaros</surname>
<given-names>S.</given-names>
</name>
<name><surname>Luscher</surname>
<given-names>T. F.</given-names>
</name>
<name><surname>Malinski</surname>
<given-names>T.</given-names>
</name>
</person-group>
<year>1996</year>
<article-title>Direct in situ measurement of nitric oxide in mesenteric resistance arteries. Increased decomposition by superoxide in hypertension</article-title>
. <source>Hypertension</source>
;
<volume>27</volume>
:<fpage>32</fpage>
-<lpage>35</lpage>
.<pub-id pub-id-type="pmid">8591884</pub-id>
</mixed-citation>
</ref>
<ref id="b53"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Vaziri</surname>
<given-names>N. D.</given-names>
</name>
<name><surname>Ni</surname>
<given-names>Z.</given-names>
</name>
<name><surname>Oveisi</surname>
<given-names>F.</given-names>
</name>
</person-group>
<year>1998</year>
<article-title>Upregulation of renal and vascular nitric oxide synthase in young spontaneously hypertensive rats</article-title>
. <source>Hypertension</source>
;
<volume>31</volume>
:<fpage>1248</fpage>
-<lpage>1254</lpage>
.<pub-id pub-id-type="pmid">9622137</pub-id>
</mixed-citation>
</ref>
<ref id="b54"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Wiinberg</surname>
<given-names>N.</given-names>
</name>
<name><surname>Hoegholm</surname>
<given-names>A.</given-names>
</name>
<name><surname>Christensen</surname>
<given-names>H. R.</given-names>
</name>
<name><surname>Bang</surname>
<given-names>L. E.</given-names>
</name>
<name><surname>Mikkelsen</surname>
<given-names>K. L.</given-names>
</name>
<name><surname>Nielsen</surname>
<given-names>P. E.</given-names>
</name>
</person-group>
<year>1995</year>
<article-title>24‐h ambulatory blood pressure in 352 normal Danish subjects, related to age and gender</article-title>
. <source>Am. J. Hypertens</source>
.;
<volume>8</volume>
10 Pt 1:<fpage>978</fpage>
-<lpage>986</lpage>
.<pub-id pub-id-type="pmid">8845079</pub-id>
</mixed-citation>
</ref>
<ref id="b55"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Yanes</surname>
<given-names>L. L.</given-names>
</name>
<name><surname>Romero</surname>
<given-names>D. G.</given-names>
</name>
<name><surname>Cucchiarelli</surname>
<given-names>V. E.</given-names>
</name>
<name><surname>Fortepiani</surname>
<given-names>L. A.</given-names>
</name>
<name><surname>Gomez‐Sanchez</surname>
<given-names>C. E.</given-names>
</name>
<name><surname>Santacruz</surname>
<given-names>F.</given-names>
</name>
</person-group>
<year>2005</year>
<article-title>Role of endothelin in mediating postmenopausal hypertension in a rat model</article-title>
. <source>Am. J. Physiol. Regul. Integr. Comp. Physiol</source>
.;
<volume>288</volume>
:<fpage>R229</fpage>
-<lpage>R233</lpage>
.<pub-id pub-id-type="pmid">15319224</pub-id>
</mixed-citation>
</ref>
</ref-list>
</back>
</pmc>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Lorraine/explor/InforLorV4/Data/Pmc/Corpus
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000088 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Pmc/Corpus/biblio.hfd -nk 000088 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Wicri/Lorraine |area= InforLorV4 |flux= Pmc |étape= Corpus |type= RBID |clé= PMC:4246578 |texte= Sex‐specific alterations in NOS regulation of vascular function in aorta and mesenteric arteries from spontaneously hypertensive rats compared to Wistar Kyoto rats }}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Pmc/Corpus/RBID.i -Sk "pubmed:25168874" \ | HfdSelect -Kh $EXPLOR_AREA/Data/Pmc/Corpus/biblio.hfd \ | NlmPubMed2Wicri -a InforLorV4
![]() | This area was generated with Dilib version V0.6.33. | ![]() |