Microencapsulation of viable hepatocytes in HEMA-MMA microcapsules: a preliminary study
Identifieur interne : 004270 ( Istex/Curation ); précédent : 004269; suivant : 004271Microencapsulation of viable hepatocytes in HEMA-MMA microcapsules: a preliminary study
Auteurs : G. D. M. Wells ; M. M. Fisher [Canada] ; M. V. SeftonSource :
- Biomaterials [ 0142-9612 ] ; 1992.
Abstract
Viable rat hepatocytes were encapsulated in a HEMA-MMA copolymer (80% HEMA). Encapsulated hepatocytes continued to produce urea (a measure of viability) for approximately 2 wk although urea production rates fell steadily over the course of in vitro culture in a pattern similar to those of control hepatocytes in conventional culture. Urea production was slightly higher in 0.01 m Tris buffered glycerol precipitated capsules, relative to phosphate buffered saline precipitated capsules. Hepatocytes were not viable in 0.001 m Tris buffered glycerol precipitated capsules which had a dense wall without the macroporosity seen in the walls of the other capsules. More work is needed to show that HEMA-MMA encapsulated hepatocytes retain some of the differentiated functions of hepatocytes.
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DOI: 10.1016/0142-9612(93)90181-Z
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G. D. M. Wells<affiliation><mods:affiliation>Department of Chemical Engineering and Applied Chemistry and Centre for Biomaterials Canada</mods:affiliation>
<wicri:noCountry code="no comma">Department of Chemical Engineering and Applied Chemistry and Centre for Biomaterials Canada</wicri:noCountry>
</affiliation>
<affiliation><mods:affiliation>Correspondence to Dr M.V. Sefton.</mods:affiliation>
<wicri:noCountry code="no comma">Correspondence to Dr M.V. Sefton.</wicri:noCountry>
</affiliation>
<affiliation><mods:affiliation>Department of Chemical Engineering and Applied Chemistry and Centre for Biomaterials Canada</mods:affiliation>
<wicri:noCountry code="no comma">Department of Chemical Engineering and Applied Chemistry and Centre for Biomaterials Canada</wicri:noCountry>
</affiliation>
<affiliation><mods:affiliation>Department of Chemical Engineering and Applied Chemistry and Centre for Biomaterials Canada</mods:affiliation>
<wicri:noCountry code="no comma">Department of Chemical Engineering and Applied Chemistry and Centre for Biomaterials Canada</wicri:noCountry>
</affiliation>
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<front><div type="abstract" xml:lang="en">Viable rat hepatocytes were encapsulated in a HEMA-MMA copolymer (80% HEMA). Encapsulated hepatocytes continued to produce urea (a measure of viability) for approximately 2 wk although urea production rates fell steadily over the course of in vitro culture in a pattern similar to those of control hepatocytes in conventional culture. Urea production was slightly higher in 0.01 m Tris buffered glycerol precipitated capsules, relative to phosphate buffered saline precipitated capsules. Hepatocytes were not viable in 0.001 m Tris buffered glycerol precipitated capsules which had a dense wall without the macroporosity seen in the walls of the other capsules. More work is needed to show that HEMA-MMA encapsulated hepatocytes retain some of the differentiated functions of hepatocytes.</div>
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