Microencapsulation of viable hepatocytes in HEMA-MMA microcapsules: a preliminary study
Identifieur interne : 004270 ( Istex/Curation ); précédent : 004269; suivant : 004271Microencapsulation of viable hepatocytes in HEMA-MMA microcapsules: a preliminary study
Auteurs : G. D. M. Wells ; M. M. Fisher [Canada] ; M. V. SeftonSource :
- Biomaterials [ 0142-9612 ] ; 1992.
Abstract
Viable rat hepatocytes were encapsulated in a HEMA-MMA copolymer (80% HEMA). Encapsulated hepatocytes continued to produce urea (a measure of viability) for approximately 2 wk although urea production rates fell steadily over the course of in vitro culture in a pattern similar to those of control hepatocytes in conventional culture. Urea production was slightly higher in 0.01 m Tris buffered glycerol precipitated capsules, relative to phosphate buffered saline precipitated capsules. Hepatocytes were not viable in 0.001 m Tris buffered glycerol precipitated capsules which had a dense wall without the macroporosity seen in the walls of the other capsules. More work is needed to show that HEMA-MMA encapsulated hepatocytes retain some of the differentiated functions of hepatocytes.
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DOI: 10.1016/0142-9612(93)90181-Z
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G. D. M. Wells<affiliation><mods:affiliation>Department of Chemical Engineering and Applied Chemistry and Centre for Biomaterials Canada</mods:affiliation><wicri:noCountry code="no comma">Department of Chemical Engineering and Applied Chemistry and Centre for Biomaterials Canada</wicri:noCountry></affiliation><affiliation><mods:affiliation>Correspondence to Dr M.V. Sefton.</mods:affiliation><wicri:noCountry code="no comma">Correspondence to Dr M.V. Sefton.</wicri:noCountry></affiliation><affiliation><mods:affiliation>Department of Chemical Engineering and Applied Chemistry and Centre for Biomaterials Canada</mods:affiliation><wicri:noCountry code="no comma">Department of Chemical Engineering and Applied Chemistry and Centre for Biomaterials Canada</wicri:noCountry></affiliation><affiliation><mods:affiliation>Department of Chemical Engineering and Applied Chemistry and Centre for Biomaterials Canada</mods:affiliation><wicri:noCountry code="no comma">Department of Chemical Engineering and Applied Chemistry and Centre for Biomaterials Canada</wicri:noCountry></affiliation>Le document en format XML
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Fisher</name><affiliation wicri:level="1"><mods:affiliation>Sunnybrook Hospital, University of Toronto, Toronto, Ontario, M5S 1A4, Canada</mods:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Sunnybrook Hospital, University of Toronto, Toronto, Ontario, M5S 1A4</wicri:regionArea></affiliation></author><author><name sortKey="Sefton, M V" sort="Sefton, M V" uniqKey="Sefton M" first="M. V." last="Sefton">M. V. Sefton</name><affiliation><mods:affiliation>Correspondence to Dr M.V. Sefton.</mods:affiliation><wicri:noCountry code="no comma">Correspondence to Dr M.V. Sefton.</wicri:noCountry></affiliation><affiliation><mods:affiliation>Department of Chemical Engineering and Applied Chemistry and Centre for Biomaterials Canada</mods:affiliation><wicri:noCountry code="no comma">Department of Chemical Engineering and Applied Chemistry and Centre for Biomaterials Canada</wicri:noCountry></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:0F98DF921F1D782AC813E2632522FF1A3CB0E315</idno><date when="1993" year="1993">1993</date><idno type="doi">10.1016/0142-9612(93)90181-Z</idno><idno type="url">https://api.istex.fr/document/0F98DF921F1D782AC813E2632522FF1A3CB0E315/fulltext/pdf</idno><idno type="wicri:Area/Istex/Corpus">004270</idno><idno type="wicri:Area/Istex/Curation">004270</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a">Microencapsulation of viable hepatocytes in HEMA-MMA microcapsules: a preliminary study</title><author><name sortKey="Wells, G D M" sort="Wells, G D M" uniqKey="Wells G" first="G. D. M." last="Wells">G. D. M. Wells</name><affiliation><mods:affiliation>Department of Chemical Engineering and Applied Chemistry and Centre for Biomaterials Canada</mods:affiliation><wicri:noCountry code="no comma">Department of Chemical Engineering and Applied Chemistry and Centre for Biomaterials Canada</wicri:noCountry></affiliation></author><author><name sortKey="Fisher, M M" sort="Fisher, M M" uniqKey="Fisher M" first="M. M." last="Fisher">M. M. Fisher</name><affiliation wicri:level="1"><mods:affiliation>Sunnybrook Hospital, University of Toronto, Toronto, Ontario, M5S 1A4, Canada</mods:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Sunnybrook Hospital, University of Toronto, Toronto, Ontario, M5S 1A4</wicri:regionArea></affiliation></author><author><name sortKey="Sefton, M V" sort="Sefton, M V" uniqKey="Sefton M" first="M. V." last="Sefton">M. V. Sefton</name><affiliation><mods:affiliation>Correspondence to Dr M.V. Sefton.</mods:affiliation><wicri:noCountry code="no comma">Correspondence to Dr M.V. Sefton.</wicri:noCountry></affiliation><affiliation><mods:affiliation>Department of Chemical Engineering and Applied Chemistry and Centre for Biomaterials Canada</mods:affiliation><wicri:noCountry code="no comma">Department of Chemical Engineering and Applied Chemistry and Centre for Biomaterials Canada</wicri:noCountry></affiliation></author></analytic><monogr></monogr><series><title level="j">Biomaterials</title><title level="j" type="abbrev">JBMT</title><idno type="ISSN">0142-9612</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1992">1992</date><biblScope unit="volume">14</biblScope><biblScope unit="issue">8</biblScope><biblScope unit="page" from="615">615</biblScope><biblScope unit="page" to="620">620</biblScope></imprint><idno type="ISSN">0142-9612</idno></series><idno type="istex">0F98DF921F1D782AC813E2632522FF1A3CB0E315</idno><idno type="DOI">10.1016/0142-9612(93)90181-Z</idno><idno type="PII">0142-9612(93)90181-Z</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0142-9612</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Viable rat hepatocytes were encapsulated in a HEMA-MMA copolymer (80% HEMA). Encapsulated hepatocytes continued to produce urea (a measure of viability) for approximately 2 wk although urea production rates fell steadily over the course of in vitro culture in a pattern similar to those of control hepatocytes in conventional culture. Urea production was slightly higher in 0.01 m Tris buffered glycerol precipitated capsules, relative to phosphate buffered saline precipitated capsules. Hepatocytes were not viable in 0.001 m Tris buffered glycerol precipitated capsules which had a dense wall without the macroporosity seen in the walls of the other capsules. More work is needed to show that HEMA-MMA encapsulated hepatocytes retain some of the differentiated functions of hepatocytes.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
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