Neuro-Muscular Differentiation of Adult Porcine Skin Derived Stem Cell-Like Cells
Identifieur interne : 000176 ( Pmc/Curation ); précédent : 000175; suivant : 000177Neuro-Muscular Differentiation of Adult Porcine Skin Derived Stem Cell-Like Cells
Auteurs : Dominik Lermen [Allemagne] ; Erwin Gorjup [Allemagne] ; Paul W. Dyce [Canada] ; Hagen Von Briesen [Allemagne] ; Paul Müller [Allemagne]Source :
- PLoS ONE [ 1932-6203 ] ; 2010.
Abstract
Due to the genetic relationship to humans, porcine stem cells are a very important model system to investigate cell differentiation, associated cell signaling pathways, and cell fate. Porcine skin derived stem cells have been isolated from mid-gestation porcine fetus recently. To our knowledge, stem cells from the skin of the adult porcine organism have not been isolated until now. Hence, to our knowledge, we here describe the isolation, expansion, characterization and differentiation of multipotent porcine skin derived stem cell-like cells (pSSCs) from the adult porcine organism for the first time.
pSSCs had a spindle shaped morphology similar to mesenchymal stem cells (MSCs). They could be maintained proliferatively active in vitro for more than 120 days and were able to form colonies from single cells. pSSCs expressed Sox2 and Oct3/4, both transcription factors essential to the pluripotent and self-renewing phenotypes of embryonic stem cells, which recently gained attention due to their function in inducing pluripotent stem cells. Furthermore, the expression of the progenitor marker nestin, the somatic stem cell markers Bcrp1/ABCG2, Bmi1, and Stat3 was detected by reverse transcriptase-polymerase chain reaction (RT-PCR) in undifferentiated pSSCs. Flow cytometry revealed the expression of the MSC related proteins CD9, CD29, CD44 and CD105, but not CD90. After neuronal differentiation cells with a characteristic morphology of neuronal and smooth muscle-like cells were present in the cultures. Subsequent immunochemistry and flow cytometry revealed the down-regulation of nestin and the up-regulation of the neuron specific protein beta-III-tubulin and the astrocyte marker GFAP. Also, alpha-SMA expressing cells increased during differentiation suggesting the neuro-muscular differentiation of these skin derived cells. pSSCs could also be induced to differentiate into adipocyte-like cells when cultured under specific conditions.
Adult porcine skin harbors a population of stem cell-like cells (pSSCs) that can be isolated via enzymatic digestion. These pSSCs show characteristic features of MSCs originated in other tissues and express the embryonic stem cell marker Oct3/4, Sox2, and Stat3. Furthermore, pSSCs have the potential to differentiate into cells from two different germ lines, the ectoderm (neurons, astrocytes) and the mesoderm (smooth muscle cells, adipocytes).
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DOI: 10.1371/journal.pone.0008968
PubMed: 20126464
PubMed Central: 2813294
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Neuro-Muscular Differentiation of Adult Porcine Skin Derived Stem Cell-Like Cells</title><author><name sortKey="Lermen, Dominik" sort="Lermen, Dominik" uniqKey="Lermen D" first="Dominik" last="Lermen">Dominik Lermen</name><affiliation wicri:level="1"><nlm:aff id="aff1"><addr-line>Department of Biogeography, Trier University, Trier, Germany</addr-line></nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Department of Biogeography, Trier University, Trier</wicri:regionArea></affiliation></author><author><name sortKey="Gorjup, Erwin" sort="Gorjup, Erwin" uniqKey="Gorjup E" first="Erwin" last="Gorjup">Erwin Gorjup</name><affiliation wicri:level="1"><nlm:aff id="aff2"><addr-line>Department of Cell Biology and Applied Virology, Fraunhofer Institute for Biomedical Engineering, St. Ingbert, Germany</addr-line></nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Department of Cell Biology and Applied Virology, Fraunhofer Institute for Biomedical Engineering, St. Ingbert</wicri:regionArea></affiliation></author><author><name sortKey="Dyce, Paul W" sort="Dyce, Paul W" uniqKey="Dyce P" first="Paul W." last="Dyce">Paul W. Dyce</name><affiliation wicri:level="1"><nlm:aff id="aff3"><addr-line>Department of Animal and Poultry Science, University of Guelph, Guelph, Ontario, Canada</addr-line></nlm:aff><country xml:lang="fr">Canada</country><wicri:regionArea>Department of Animal and Poultry Science, University of Guelph, Guelph, Ontario</wicri:regionArea></affiliation></author><author><name sortKey="Von Briesen, Hagen" sort="Von Briesen, Hagen" uniqKey="Von Briesen H" first="Hagen" last="Von Briesen">Hagen Von Briesen</name><affiliation wicri:level="1"><nlm:aff id="aff2"><addr-line>Department of Cell Biology and Applied Virology, Fraunhofer Institute for Biomedical Engineering, St. Ingbert, Germany</addr-line></nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Department of Cell Biology and Applied Virology, Fraunhofer Institute for Biomedical Engineering, St. Ingbert</wicri:regionArea></affiliation></author><author><name sortKey="Muller, Paul" sort="Muller, Paul" uniqKey="Muller P" first="Paul" last="Müller">Paul Müller</name><affiliation wicri:level="1"><nlm:aff id="aff1"><addr-line>Department of Biogeography, Trier University, Trier, Germany</addr-line></nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Department of Biogeography, Trier University, Trier</wicri:regionArea></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">20126464</idno><idno type="pmc">2813294</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2813294</idno><idno type="RBID">PMC:2813294</idno><idno type="doi">10.1371/journal.pone.0008968</idno><date when="2010">2010</date><idno type="wicri:Area/Pmc/Corpus">000176</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000176</idno><idno type="wicri:Area/Pmc/Curation">000176</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000176</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Neuro-Muscular Differentiation of Adult Porcine Skin Derived Stem Cell-Like Cells</title><author><name sortKey="Lermen, Dominik" sort="Lermen, Dominik" uniqKey="Lermen D" first="Dominik" last="Lermen">Dominik Lermen</name><affiliation wicri:level="1"><nlm:aff id="aff1"><addr-line>Department of Biogeography, Trier University, Trier, Germany</addr-line></nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Department of Biogeography, Trier University, Trier</wicri:regionArea></affiliation></author><author><name sortKey="Gorjup, Erwin" sort="Gorjup, Erwin" uniqKey="Gorjup E" first="Erwin" last="Gorjup">Erwin Gorjup</name><affiliation wicri:level="1"><nlm:aff id="aff2"><addr-line>Department of Cell Biology and Applied Virology, Fraunhofer Institute for Biomedical Engineering, St. Ingbert, Germany</addr-line></nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Department of Cell Biology and Applied Virology, Fraunhofer Institute for Biomedical Engineering, St. Ingbert</wicri:regionArea></affiliation></author><author><name sortKey="Dyce, Paul W" sort="Dyce, Paul W" uniqKey="Dyce P" first="Paul W." last="Dyce">Paul W. Dyce</name><affiliation wicri:level="1"><nlm:aff id="aff3"><addr-line>Department of Animal and Poultry Science, University of Guelph, Guelph, Ontario, Canada</addr-line></nlm:aff><country xml:lang="fr">Canada</country><wicri:regionArea>Department of Animal and Poultry Science, University of Guelph, Guelph, Ontario</wicri:regionArea></affiliation></author><author><name sortKey="Von Briesen, Hagen" sort="Von Briesen, Hagen" uniqKey="Von Briesen H" first="Hagen" last="Von Briesen">Hagen Von Briesen</name><affiliation wicri:level="1"><nlm:aff id="aff2"><addr-line>Department of Cell Biology and Applied Virology, Fraunhofer Institute for Biomedical Engineering, St. Ingbert, Germany</addr-line></nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Department of Cell Biology and Applied Virology, Fraunhofer Institute for Biomedical Engineering, St. Ingbert</wicri:regionArea></affiliation></author><author><name sortKey="Muller, Paul" sort="Muller, Paul" uniqKey="Muller P" first="Paul" last="Müller">Paul Müller</name><affiliation wicri:level="1"><nlm:aff id="aff1"><addr-line>Department of Biogeography, Trier University, Trier, Germany</addr-line></nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Department of Biogeography, Trier University, Trier</wicri:regionArea></affiliation></author></analytic><series><title level="j">PLoS ONE</title><idno type="eISSN">1932-6203</idno><imprint><date when="2010">2010</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><sec><title>Background</title><p>Due to the genetic relationship to humans, porcine stem cells are a very important model system to investigate cell differentiation, associated cell signaling pathways, and cell fate. Porcine skin derived stem cells have been isolated from mid-gestation porcine fetus recently. To our knowledge, stem cells from the skin of the adult porcine organism have not been isolated until now. Hence, to our knowledge, we here describe the isolation, expansion, characterization and differentiation of multipotent porcine skin derived stem cell-like cells (pSSCs) from the adult porcine organism for the first time.</p></sec><sec><title>Methodology/Principal Findings</title><p>pSSCs had a spindle shaped morphology similar to mesenchymal stem cells (MSCs). They could be maintained proliferatively active in vitro for more than 120 days and were able to form colonies from single cells. pSSCs expressed Sox2 and Oct3/4, both transcription factors essential to the pluripotent and self-renewing phenotypes of embryonic stem cells, which recently gained attention due to their function in inducing pluripotent stem cells. Furthermore, the expression of the progenitor marker nestin, the somatic stem cell markers Bcrp1/ABCG2, Bmi1, and Stat3 was detected by reverse transcriptase-polymerase chain reaction (RT-PCR) in undifferentiated pSSCs. Flow cytometry revealed the expression of the MSC related proteins CD9, CD29, CD44 and CD105, but not CD90. After neuronal differentiation cells with a characteristic morphology of neuronal and smooth muscle-like cells were present in the cultures. Subsequent immunochemistry and flow cytometry revealed the down-regulation of nestin and the up-regulation of the neuron specific protein beta-III-tubulin and the astrocyte marker GFAP. Also, alpha-SMA expressing cells increased during differentiation suggesting the neuro-muscular differentiation of these skin derived cells. pSSCs could also be induced to differentiate into adipocyte-like cells when cultured under specific conditions.</p></sec><sec><title>Conclusions/Significance</title><p>Adult porcine skin harbors a population of stem cell-like cells (pSSCs) that can be isolated via enzymatic digestion. These pSSCs show characteristic features of MSCs originated in other tissues and express the embryonic stem cell marker Oct3/4, Sox2, and Stat3. 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<front><journal-meta><journal-id journal-id-type="nlm-ta">PLoS One</journal-id><journal-id journal-id-type="publisher-id">plos</journal-id><journal-id journal-id-type="pmc">plosone</journal-id><journal-title-group><journal-title>PLoS ONE</journal-title></journal-title-group><issn pub-type="epub">1932-6203</issn><publisher><publisher-name>Public Library of Science</publisher-name><publisher-loc>San Francisco, USA</publisher-loc></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">20126464</article-id><article-id pub-id-type="pmc">2813294</article-id><article-id pub-id-type="publisher-id">09-PONE-RA-14774</article-id><article-id pub-id-type="doi">10.1371/journal.pone.0008968</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group><subj-group subj-group-type="Discipline"><subject>Cell Biology</subject><subject>Dermatology</subject><subject>Developmental Biology/Cell Differentiation</subject><subject>Developmental Biology/Stem Cells</subject><subject>Ecology/Conservation and Restoration Ecology</subject></subj-group></article-categories><title-group><article-title>Neuro-Muscular Differentiation of Adult Porcine Skin Derived Stem Cell-Like Cells</article-title><alt-title alt-title-type="running-head">Stem Cells from Porcine Skin</alt-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Lermen</surname><given-names>Dominik</given-names></name><xref ref-type="aff" rid="aff1"><sup>1</sup></xref><xref ref-type="corresp" rid="cor1"><sup>*</sup></xref></contrib><contrib contrib-type="author"><name><surname>Gorjup</surname><given-names>Erwin</given-names></name><xref ref-type="aff" rid="aff2"><sup>2</sup></xref></contrib><contrib contrib-type="author"><name><surname>Dyce</surname><given-names>Paul W.</given-names></name><xref ref-type="aff" rid="aff3"><sup>3</sup></xref></contrib><contrib contrib-type="author"><name><surname>von Briesen</surname><given-names>Hagen</given-names></name><xref ref-type="aff" rid="aff2"><sup>2</sup></xref></contrib><contrib contrib-type="author"><name><surname>Müller</surname><given-names>Paul</given-names></name><xref ref-type="aff" rid="aff1"><sup>1</sup></xref></contrib></contrib-group><aff id="aff1"><label>1</label><addr-line>Department of Biogeography, Trier University, Trier, Germany</addr-line></aff><aff id="aff2"><label>2</label><addr-line>Department of Cell Biology and Applied Virology, Fraunhofer Institute for Biomedical Engineering, St. Ingbert, Germany</addr-line></aff><aff id="aff3"><label>3</label><addr-line>Department of Animal and Poultry Science, University of Guelph, Guelph, Ontario, Canada</addr-line></aff><contrib-group><contrib contrib-type="editor"><name><surname>Aziz</surname><given-names>Syed A.</given-names></name><role>Editor</role><xref ref-type="aff" rid="edit1"></xref></contrib></contrib-group><aff id="edit1">Health Canada, Canada</aff><author-notes><corresp id="cor1">* E-mail: <email>lermen@uni-trier.de</email></corresp><fn fn-type="con"><p>Conceived and designed the experiments: DL EG PD HvB PM. Performed the experiments: DL EG. Analyzed the data: DL EG PD HvB PM. Contributed reagents/materials/analysis tools: HvB PM. Wrote the paper: DL. Final approval of the manuscript: DL HvB PM.</p></fn></author-notes><pub-date pub-type="collection"><year>2010</year></pub-date><pub-date pub-type="epub"><day>29</day><month>1</month><year>2010</year></pub-date><volume>5</volume><issue>1</issue><elocation-id>e8968</elocation-id><history><date date-type="received"><day>9</day><month>12</month><year>2009</year></date><date date-type="accepted"><day>13</day><month>1</month><year>2010</year></date></history><permissions><copyright-statement>Lermen et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.</copyright-statement></permissions><abstract><sec><title>Background</title><p>Due to the genetic relationship to humans, porcine stem cells are a very important model system to investigate cell differentiation, associated cell signaling pathways, and cell fate. Porcine skin derived stem cells have been isolated from mid-gestation porcine fetus recently. To our knowledge, stem cells from the skin of the adult porcine organism have not been isolated until now. Hence, to our knowledge, we here describe the isolation, expansion, characterization and differentiation of multipotent porcine skin derived stem cell-like cells (pSSCs) from the adult porcine organism for the first time.</p></sec><sec><title>Methodology/Principal Findings</title><p>pSSCs had a spindle shaped morphology similar to mesenchymal stem cells (MSCs). They could be maintained proliferatively active in vitro for more than 120 days and were able to form colonies from single cells. pSSCs expressed Sox2 and Oct3/4, both transcription factors essential to the pluripotent and self-renewing phenotypes of embryonic stem cells, which recently gained attention due to their function in inducing pluripotent stem cells. Furthermore, the expression of the progenitor marker nestin, the somatic stem cell markers Bcrp1/ABCG2, Bmi1, and Stat3 was detected by reverse transcriptase-polymerase chain reaction (RT-PCR) in undifferentiated pSSCs. Flow cytometry revealed the expression of the MSC related proteins CD9, CD29, CD44 and CD105, but not CD90. After neuronal differentiation cells with a characteristic morphology of neuronal and smooth muscle-like cells were present in the cultures. Subsequent immunochemistry and flow cytometry revealed the down-regulation of nestin and the up-regulation of the neuron specific protein beta-III-tubulin and the astrocyte marker GFAP. Also, alpha-SMA expressing cells increased during differentiation suggesting the neuro-muscular differentiation of these skin derived cells. pSSCs could also be induced to differentiate into adipocyte-like cells when cultured under specific conditions.</p></sec><sec><title>Conclusions/Significance</title><p>Adult porcine skin harbors a population of stem cell-like cells (pSSCs) that can be isolated via enzymatic digestion. These pSSCs show characteristic features of MSCs originated in other tissues and express the embryonic stem cell marker Oct3/4, Sox2, and Stat3. Furthermore, pSSCs have the potential to differentiate into cells from two different germ lines, the ectoderm (neurons, astrocytes) and the mesoderm (smooth muscle cells, adipocytes).</p></sec></abstract><counts><page-count count="9"></page-count></counts></article-meta></front></pmc></record>Pour manipuler ce document sous Unix (Dilib)
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