Expression of RAS-like family members, c-jun and c-myc mRNA levels in neoplastic hemocytes of soft-shell clams Mya arenaria using microsphere-based 8-plex branched DNA assay.
Identifieur interne : 000190 ( PubMed/Corpus ); précédent : 000189; suivant : 000191Expression of RAS-like family members, c-jun and c-myc mRNA levels in neoplastic hemocytes of soft-shell clams Mya arenaria using microsphere-based 8-plex branched DNA assay.
Auteurs : A. Siah ; P. Mckenna ; J M Danger ; G. Johnson ; F C J. BertheSource :
- Results in immunology ; 2012.
Abstract
The molecular mechanisms by which disseminated neoplasia (DN) is developed in soft shell clams Mya arenaria remain largely unknown. This study aims at quantifying Rho-like GTPase, RAS-Rho, RAS-related C3 botulinum (RAS C3), c-jun as well as c-myc transcript levels in clams sampled at North River (Charlottetown, Prince Edward Island, Canada). The transcripts were quantified using multiplex gene analysis (Quantigene(®) 2 Plex, Affymetrix) in 3 groups of clams: (1) Group C (healthy clams considered as control) with a low percentage of tetraploid hemocytes (<10%); (2) Group D (disease in development): individuals presenting a percentage of tetraploid cells ranging between 10% and 50%; (3) Group E (established disease): clams with a high percentage of tetraploid hemocytes (>50%). Data showed a down-regulation of Rho-like GTPase, Rho-like subfamily, RAS C3, c-jun and an up-regulation of c-myc gene expression. It is believed that a deregulation of the expression of these genes could partly unravel the molecular mechanisms involved in the development of DN in soft shell clams Mya arenaria. Further investigations should be pursued to determine the role of these gene products in clams' hemocytes.
DOI: 10.1016/j.rinim.2012.03.003
PubMed: 24371570
Links to Exploration step
pubmed:24371570Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Expression of RAS-like family members, c-jun and c-myc mRNA levels in neoplastic hemocytes of soft-shell clams Mya arenaria using microsphere-based 8-plex branched DNA assay.</title><author><name sortKey="Siah, A" sort="Siah, A" uniqKey="Siah A" first="A" last="Siah">A. Siah</name><affiliation><nlm:affiliation>British Columbia Centre for Aquatic Health Sciences, 871A Island Highway, Campbell River, BC, Canada V9W 2C2 ; Department of Pathology & Microbiology, Atlantic Veterinary College, University of Prince Edward Island, 220 University Avenue, Charlottetown, PEI, Canada C1A 4P3.</nlm:affiliation></affiliation></author><author><name sortKey="Mckenna, P" sort="Mckenna, P" uniqKey="Mckenna P" first="P" last="Mckenna">P. Mckenna</name><affiliation><nlm:affiliation>Department of Pathology & Microbiology, Atlantic Veterinary College, University of Prince Edward Island, 220 University Avenue, Charlottetown, PEI, Canada C1A 4P3.</nlm:affiliation></affiliation></author><author><name sortKey="Danger, J M" sort="Danger, J M" uniqKey="Danger J" first="J M" last="Danger">J M Danger</name><affiliation><nlm:affiliation>Laboratory of Ecotoxicology, University of Le Havre, 25 rue P. Lebon, BP 540, 76058 Le Havre Cedex, France.</nlm:affiliation></affiliation></author><author><name sortKey="Johnson, G" sort="Johnson, G" uniqKey="Johnson G" first="G" last="Johnson">G. Johnson</name><affiliation><nlm:affiliation>Department of Pathology & Microbiology, Atlantic Veterinary College, University of Prince Edward Island, 220 University Avenue, Charlottetown, PEI, Canada C1A 4P3.</nlm:affiliation></affiliation></author><author><name sortKey="Berthe, F C J" sort="Berthe, F C J" uniqKey="Berthe F" first="F C J" last="Berthe">F C J. Berthe</name><affiliation><nlm:affiliation>Animal Health and Welfare Unit, European Food Safety Authority (EFSA), Largo N, Palli 5IA, I-43100 Parma, Italy.</nlm:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2012">2012</date><idno type="RBID">pubmed:24371570</idno><idno type="pmid">24371570</idno><idno type="doi">10.1016/j.rinim.2012.03.003</idno><idno type="wicri:Area/PubMed/Corpus">000190</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Expression of RAS-like family members, c-jun and c-myc mRNA levels in neoplastic hemocytes of soft-shell clams Mya arenaria using microsphere-based 8-plex branched DNA assay.</title><author><name sortKey="Siah, A" sort="Siah, A" uniqKey="Siah A" first="A" last="Siah">A. Siah</name><affiliation><nlm:affiliation>British Columbia Centre for Aquatic Health Sciences, 871A Island Highway, Campbell River, BC, Canada V9W 2C2 ; Department of Pathology & Microbiology, Atlantic Veterinary College, University of Prince Edward Island, 220 University Avenue, Charlottetown, PEI, Canada C1A 4P3.</nlm:affiliation></affiliation></author><author><name sortKey="Mckenna, P" sort="Mckenna, P" uniqKey="Mckenna P" first="P" last="Mckenna">P. Mckenna</name><affiliation><nlm:affiliation>Department of Pathology & Microbiology, Atlantic Veterinary College, University of Prince Edward Island, 220 University Avenue, Charlottetown, PEI, Canada C1A 4P3.</nlm:affiliation></affiliation></author><author><name sortKey="Danger, J M" sort="Danger, J M" uniqKey="Danger J" first="J M" last="Danger">J M Danger</name><affiliation><nlm:affiliation>Laboratory of Ecotoxicology, University of Le Havre, 25 rue P. Lebon, BP 540, 76058 Le Havre Cedex, France.</nlm:affiliation></affiliation></author><author><name sortKey="Johnson, G" sort="Johnson, G" uniqKey="Johnson G" first="G" last="Johnson">G. Johnson</name><affiliation><nlm:affiliation>Department of Pathology & Microbiology, Atlantic Veterinary College, University of Prince Edward Island, 220 University Avenue, Charlottetown, PEI, Canada C1A 4P3.</nlm:affiliation></affiliation></author><author><name sortKey="Berthe, F C J" sort="Berthe, F C J" uniqKey="Berthe F" first="F C J" last="Berthe">F C J. Berthe</name><affiliation><nlm:affiliation>Animal Health and Welfare Unit, European Food Safety Authority (EFSA), Largo N, Palli 5IA, I-43100 Parma, Italy.</nlm:affiliation></affiliation></author></analytic><series><title level="j">Results in immunology</title><imprint><date when="2012" type="published">2012</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The molecular mechanisms by which disseminated neoplasia (DN) is developed in soft shell clams Mya arenaria remain largely unknown. This study aims at quantifying Rho-like GTPase, RAS-Rho, RAS-related C3 botulinum (RAS C3), c-jun as well as c-myc transcript levels in clams sampled at North River (Charlottetown, Prince Edward Island, Canada). The transcripts were quantified using multiplex gene analysis (Quantigene(®) 2 Plex, Affymetrix) in 3 groups of clams: (1) Group C (healthy clams considered as control) with a low percentage of tetraploid hemocytes (<10%); (2) Group D (disease in development): individuals presenting a percentage of tetraploid cells ranging between 10% and 50%; (3) Group E (established disease): clams with a high percentage of tetraploid hemocytes (>50%). Data showed a down-regulation of Rho-like GTPase, Rho-like subfamily, RAS C3, c-jun and an up-regulation of c-myc gene expression. It is believed that a deregulation of the expression of these genes could partly unravel the molecular mechanisms involved in the development of DN in soft shell clams Mya arenaria. Further investigations should be pursued to determine the role of these gene products in clams' hemocytes.</div></front></TEI><pubmed><MedlineCitation Status="PubMed-not-MEDLINE" Owner="NLM"><PMID Version="1">24371570</PMID><DateCreated><Year>2013</Year><Month>12</Month><Day>27</Day></DateCreated><DateCompleted><Year>2013</Year><Month>12</Month><Day>27</Day></DateCompleted><DateRevised><Year>2014</Year><Month>1</Month><Day>24</Day></DateRevised><Article PubModel="Electronic-eCollection"><Journal><JournalIssue CitedMedium="Print"><Volume>2</Volume><PubDate><Year>2012</Year></PubDate></JournalIssue><Title>Results in immunology</Title><ISOAbbreviation>Results Immunol</ISOAbbreviation></Journal><ArticleTitle>Expression of RAS-like family members, c-jun and c-myc mRNA levels in neoplastic hemocytes of soft-shell clams Mya arenaria using microsphere-based 8-plex branched DNA assay.</ArticleTitle><Pagination><MedlinePgn>83-7</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.rinim.2012.03.003</ELocationID><Abstract><AbstractText>The molecular mechanisms by which disseminated neoplasia (DN) is developed in soft shell clams Mya arenaria remain largely unknown. This study aims at quantifying Rho-like GTPase, RAS-Rho, RAS-related C3 botulinum (RAS C3), c-jun as well as c-myc transcript levels in clams sampled at North River (Charlottetown, Prince Edward Island, Canada). The transcripts were quantified using multiplex gene analysis (Quantigene(®) 2 Plex, Affymetrix) in 3 groups of clams: (1) Group C (healthy clams considered as control) with a low percentage of tetraploid hemocytes (<10%); (2) Group D (disease in development): individuals presenting a percentage of tetraploid cells ranging between 10% and 50%; (3) Group E (established disease): clams with a high percentage of tetraploid hemocytes (>50%). Data showed a down-regulation of Rho-like GTPase, Rho-like subfamily, RAS C3, c-jun and an up-regulation of c-myc gene expression. It is believed that a deregulation of the expression of these genes could partly unravel the molecular mechanisms involved in the development of DN in soft shell clams Mya arenaria. Further investigations should be pursued to determine the role of these gene products in clams' hemocytes.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Siah</LastName><ForeName>A</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>British Columbia Centre for Aquatic Health Sciences, 871A Island Highway, Campbell River, BC, Canada V9W 2C2 ; Department of Pathology & Microbiology, Atlantic Veterinary College, University of Prince Edward Island, 220 University Avenue, Charlottetown, PEI, Canada C1A 4P3.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>McKenna</LastName><ForeName>P</ForeName><Initials>P</Initials><AffiliationInfo><Affiliation>Department of Pathology & Microbiology, Atlantic Veterinary College, University of Prince Edward Island, 220 University Avenue, Charlottetown, PEI, Canada C1A 4P3.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Danger</LastName><ForeName>J M</ForeName><Initials>JM</Initials><AffiliationInfo><Affiliation>Laboratory of Ecotoxicology, University of Le Havre, 25 rue P. Lebon, BP 540, 76058 Le Havre Cedex, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Johnson</LastName><ForeName>G</ForeName><Initials>G</Initials><AffiliationInfo><Affiliation>Department of Pathology & Microbiology, Atlantic Veterinary College, University of Prince Edward Island, 220 University Avenue, Charlottetown, PEI, Canada C1A 4P3.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Berthe</LastName><ForeName>F C J</ForeName><Initials>FC</Initials><AffiliationInfo><Affiliation>Animal Health and Welfare Unit, European Food Safety Authority (EFSA), Largo N, Palli 5IA, I-43100 Parma, Italy.</Affiliation></AffiliationInfo></Author></AuthorList><Language>ENG</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2012</Year><Month>Apr</Month><Day>07</Day></ArticleDate></Article><MedlineJournalInfo><Country>Netherlands</Country><MedlineTA>Results Immunol</MedlineTA><NlmUniqueID>101568318</NlmUniqueID><ISSNLinking>2211-2839</ISSNLinking></MedlineJournalInfo><OtherID Source="NLM">PMC3862338</OtherID><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Hemocytes</Keyword><Keyword MajorTopicYN="N">Multiplex</Keyword><Keyword MajorTopicYN="N">RAS</Keyword><Keyword MajorTopicYN="N">Tetraploidy</Keyword><Keyword MajorTopicYN="N">c-jun</Keyword><Keyword MajorTopicYN="N">c-myc</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2012</Year><Month>2</Month><Day>16</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2012</Year><Month>3</Month><Day>20</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2012</Year><Month>3</Month><Day>29</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2013</Year><Month>12</Month><Day>28</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2012</Year><Month>1</Month><Day>1</Day><Hour>0</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2012</Year><Month>1</Month><Day>1</Day><Hour>0</Hour><Minute>1</Minute></PubMedPubDate></History><PublicationStatus>epublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">24371570</ArticleId><ArticleId IdType="doi">10.1016/j.rinim.2012.03.003</ArticleId><ArticleId IdType="pii">S2211-2839(12)00010-X</ArticleId><ArticleId IdType="pmc">PMC3862338</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/France/explor/LeHavreV1/Data/PubMed/Corpus
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000190 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/PubMed/Corpus/biblio.hfd -nk 000190 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/France
|area= LeHavreV1
|flux= PubMed
|étape= Corpus
|type= RBID
|clé= pubmed:24371570
|texte= Expression of RAS-like family members, c-jun and c-myc mRNA levels in neoplastic hemocytes of soft-shell clams Mya arenaria using microsphere-based 8-plex branched DNA assay.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/PubMed/Corpus/RBID.i -Sk "pubmed:24371570" \
| HfdSelect -Kh $EXPLOR_AREA/Data/PubMed/Corpus/biblio.hfd \
| NlmPubMed2Wicri -a LeHavreV1
| This area was generated with Dilib version V0.6.25. |  |