Dietary docosahexaenoic acid-enriched glycerophospholipids exert cardioprotective effects in ouabain-treated rats via physiological and metabolic changes.
Identifieur interne : 000055 ( PubMed/Corpus ); précédent : 000054; suivant : 000056Dietary docosahexaenoic acid-enriched glycerophospholipids exert cardioprotective effects in ouabain-treated rats via physiological and metabolic changes.
Auteurs : Monique Bernard ; Jean-Michel Maixent ; Alain Gerbi ; Carole Lan ; Patrick Jean Cozzone ; Gérard Pieroni ; Martine Armand ; Thierry Charles CosteSource :
- Food & function [ 2042-650X ] ; 2016.
Abstract
Docosahexaenoic acid (DHA) might prevent heart failure or optimise drug treatments by improving cardiac contraction. We investigated whether DHA-enriched avian glycerophospholipids (GPL-DHA) exert cardioprotection in ouabain-treated rats after 4 weeks of dietary supplementation with 10, 35 or 60 mg DHA per kg body weight versus none (DHA10, DHA35, DHA60 and control groups, respectively). The contractile responsiveness to different doses of ouabain (10(-7) to 10(-4) M), ouabain intoxication (at 3 × 10(-4) M), and relative variations in cardiac energy metabolism were determined using (31)P NMR in isolated perfused rat hearts. The fatty acid composition of cardiac membranes was analysed by gas chromatography. DHA accretion in the heart was dose-dependent (+8%, +30% and +45% for DHA10, DHA35 and DHA60, respectively). The cardiac phosphocreatine content significantly increased at the baseline in DHA35 (+45%) and DHA60 groups (+85%), and at the different doses of ouabain in the DHA60 group (+73% to 98%). The maximum positive inotropy achieved at 10(-4) M ouabain was significantly increased in all DHA groups versus control (+150%, +122.5% and +135% for DHA10, DHA35 and DHA60, respectively), and ouabain intoxication was delayed. The increase in myocardial phosphocreatine content and the improved efficacy of ouabain on myocardial contraction without toxicity suggest the potential of GPL-DHA as a dietary supplement or ingredient for functional food, and possibly as a co-treatment with digitalis drugs in humans.
DOI: 10.1039/c5fo01300c
PubMed: 26662260
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Dietary docosahexaenoic acid-enriched glycerophospholipids exert cardioprotective effects in ouabain-treated rats via physiological and metabolic changes.</title><author><name sortKey="Bernard, Monique" sort="Bernard, Monique" uniqKey="Bernard M" first="Monique" last="Bernard">Monique Bernard</name><affiliation><nlm:affiliation>Aix-Marseille Université, CNRS, CRMBM UMR 7339, F-13385 Marseille, France. monique.bernard@univ-amu.fr.</nlm:affiliation></affiliation></author><author><name sortKey="Maixent, Jean Michel" sort="Maixent, Jean Michel" uniqKey="Maixent J" first="Jean-Michel" last="Maixent">Jean-Michel Maixent</name><affiliation><nlm:affiliation>EB2M-Protée, Université du Sud Toulon-Var, F-83957 La Garde, France.</nlm:affiliation></affiliation></author><author><name sortKey="Gerbi, Alain" sort="Gerbi, Alain" uniqKey="Gerbi A" first="Alain" last="Gerbi">Alain Gerbi</name><affiliation><nlm:affiliation>RDVC Produits Santé, F-76600 Le Havre, France.</nlm:affiliation></affiliation></author><author><name sortKey="Lan, Carole" sort="Lan, Carole" uniqKey="Lan C" first="Carole" last="Lan">Carole Lan</name><affiliation><nlm:affiliation>Aix-Marseille Université, CNRS, CRMBM UMR 7339, F-13385 Marseille, France. monique.bernard@univ-amu.fr.</nlm:affiliation></affiliation></author><author><name sortKey="Cozzone, Patrick Jean" sort="Cozzone, Patrick Jean" uniqKey="Cozzone P" first="Patrick Jean" last="Cozzone">Patrick Jean Cozzone</name><affiliation><nlm:affiliation>Aix-Marseille Université, CNRS, CRMBM UMR 7339, F-13385 Marseille, France. monique.bernard@univ-amu.fr.</nlm:affiliation></affiliation></author><author><name sortKey="Pieroni, Gerard" sort="Pieroni, Gerard" uniqKey="Pieroni G" first="Gérard" last="Pieroni">Gérard Pieroni</name><affiliation><nlm:affiliation>Application Santé des Lipides, ASL, Bioparc de Vichy, F-03270 Hauterive, France.</nlm:affiliation></affiliation></author><author><name sortKey="Armand, Martine" sort="Armand, Martine" uniqKey="Armand M" first="Martine" last="Armand">Martine Armand</name><affiliation><nlm:affiliation>Aix-Marseille Université, CNRS, CRMBM UMR 7339, F-13385 Marseille, France. monique.bernard@univ-amu.fr.</nlm:affiliation></affiliation></author><author><name sortKey="Coste, Thierry Charles" sort="Coste, Thierry Charles" uniqKey="Coste T" first="Thierry Charles" last="Coste">Thierry Charles Coste</name><affiliation><nlm:affiliation>Novastell, F-27150 Etrépagny, France.</nlm:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2016">2016</date><idno type="RBID">pubmed:26662260</idno><idno type="pmid">26662260</idno><idno type="doi">10.1039/c5fo01300c</idno><idno type="wicri:Area/PubMed/Corpus">000055</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Dietary docosahexaenoic acid-enriched glycerophospholipids exert cardioprotective effects in ouabain-treated rats via physiological and metabolic changes.</title><author><name sortKey="Bernard, Monique" sort="Bernard, Monique" uniqKey="Bernard M" first="Monique" last="Bernard">Monique Bernard</name><affiliation><nlm:affiliation>Aix-Marseille Université, CNRS, CRMBM UMR 7339, F-13385 Marseille, France. monique.bernard@univ-amu.fr.</nlm:affiliation></affiliation></author><author><name sortKey="Maixent, Jean Michel" sort="Maixent, Jean Michel" uniqKey="Maixent J" first="Jean-Michel" last="Maixent">Jean-Michel Maixent</name><affiliation><nlm:affiliation>EB2M-Protée, Université du Sud Toulon-Var, F-83957 La Garde, France.</nlm:affiliation></affiliation></author><author><name sortKey="Gerbi, Alain" sort="Gerbi, Alain" uniqKey="Gerbi A" first="Alain" last="Gerbi">Alain Gerbi</name><affiliation><nlm:affiliation>RDVC Produits Santé, F-76600 Le Havre, France.</nlm:affiliation></affiliation></author><author><name sortKey="Lan, Carole" sort="Lan, Carole" uniqKey="Lan C" first="Carole" last="Lan">Carole Lan</name><affiliation><nlm:affiliation>Aix-Marseille Université, CNRS, CRMBM UMR 7339, F-13385 Marseille, France. monique.bernard@univ-amu.fr.</nlm:affiliation></affiliation></author><author><name sortKey="Cozzone, Patrick Jean" sort="Cozzone, Patrick Jean" uniqKey="Cozzone P" first="Patrick Jean" last="Cozzone">Patrick Jean Cozzone</name><affiliation><nlm:affiliation>Aix-Marseille Université, CNRS, CRMBM UMR 7339, F-13385 Marseille, France. monique.bernard@univ-amu.fr.</nlm:affiliation></affiliation></author><author><name sortKey="Pieroni, Gerard" sort="Pieroni, Gerard" uniqKey="Pieroni G" first="Gérard" last="Pieroni">Gérard Pieroni</name><affiliation><nlm:affiliation>Application Santé des Lipides, ASL, Bioparc de Vichy, F-03270 Hauterive, France.</nlm:affiliation></affiliation></author><author><name sortKey="Armand, Martine" sort="Armand, Martine" uniqKey="Armand M" first="Martine" last="Armand">Martine Armand</name><affiliation><nlm:affiliation>Aix-Marseille Université, CNRS, CRMBM UMR 7339, F-13385 Marseille, France. monique.bernard@univ-amu.fr.</nlm:affiliation></affiliation></author><author><name sortKey="Coste, Thierry Charles" sort="Coste, Thierry Charles" uniqKey="Coste T" first="Thierry Charles" last="Coste">Thierry Charles Coste</name><affiliation><nlm:affiliation>Novastell, F-27150 Etrépagny, France.</nlm:affiliation></affiliation></author></analytic><series><title level="j">Food & function</title><idno type="eISSN">2042-650X</idno><imprint><date when="2016" type="published">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Docosahexaenoic acid (DHA) might prevent heart failure or optimise drug treatments by improving cardiac contraction. We investigated whether DHA-enriched avian glycerophospholipids (GPL-DHA) exert cardioprotection in ouabain-treated rats after 4 weeks of dietary supplementation with 10, 35 or 60 mg DHA per kg body weight versus none (DHA10, DHA35, DHA60 and control groups, respectively). The contractile responsiveness to different doses of ouabain (10(-7) to 10(-4) M), ouabain intoxication (at 3 × 10(-4) M), and relative variations in cardiac energy metabolism were determined using (31)P NMR in isolated perfused rat hearts. The fatty acid composition of cardiac membranes was analysed by gas chromatography. DHA accretion in the heart was dose-dependent (+8%, +30% and +45% for DHA10, DHA35 and DHA60, respectively). The cardiac phosphocreatine content significantly increased at the baseline in DHA35 (+45%) and DHA60 groups (+85%), and at the different doses of ouabain in the DHA60 group (+73% to 98%). The maximum positive inotropy achieved at 10(-4) M ouabain was significantly increased in all DHA groups versus control (+150%, +122.5% and +135% for DHA10, DHA35 and DHA60, respectively), and ouabain intoxication was delayed. The increase in myocardial phosphocreatine content and the improved efficacy of ouabain on myocardial contraction without toxicity suggest the potential of GPL-DHA as a dietary supplement or ingredient for functional food, and possibly as a co-treatment with digitalis drugs in humans.</div></front></TEI><pubmed><MedlineCitation Status="In-Process" Owner="NLM"><PMID Version="1">26662260</PMID><DateCreated><Year>2016</Year><Month>2</Month><Day>18</Day></DateCreated><DateRevised><Year>2016</Year><Month>2</Month><Day>18</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Electronic">2042-650X</ISSN><JournalIssue CitedMedium="Internet"><Volume>7</Volume><Issue>2</Issue><PubDate><Year>2016</Year><Month>Feb</Month></PubDate></JournalIssue><Title>Food & function</Title><ISOAbbreviation>Food Funct</ISOAbbreviation></Journal><ArticleTitle>Dietary docosahexaenoic acid-enriched glycerophospholipids exert cardioprotective effects in ouabain-treated rats via physiological and metabolic changes.</ArticleTitle><Pagination><MedlinePgn>798-804</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1039/c5fo01300c</ELocationID><Abstract><AbstractText>Docosahexaenoic acid (DHA) might prevent heart failure or optimise drug treatments by improving cardiac contraction. We investigated whether DHA-enriched avian glycerophospholipids (GPL-DHA) exert cardioprotection in ouabain-treated rats after 4 weeks of dietary supplementation with 10, 35 or 60 mg DHA per kg body weight versus none (DHA10, DHA35, DHA60 and control groups, respectively). The contractile responsiveness to different doses of ouabain (10(-7) to 10(-4) M), ouabain intoxication (at 3 × 10(-4) M), and relative variations in cardiac energy metabolism were determined using (31)P NMR in isolated perfused rat hearts. The fatty acid composition of cardiac membranes was analysed by gas chromatography. DHA accretion in the heart was dose-dependent (+8%, +30% and +45% for DHA10, DHA35 and DHA60, respectively). The cardiac phosphocreatine content significantly increased at the baseline in DHA35 (+45%) and DHA60 groups (+85%), and at the different doses of ouabain in the DHA60 group (+73% to 98%). The maximum positive inotropy achieved at 10(-4) M ouabain was significantly increased in all DHA groups versus control (+150%, +122.5% and +135% for DHA10, DHA35 and DHA60, respectively), and ouabain intoxication was delayed. The increase in myocardial phosphocreatine content and the improved efficacy of ouabain on myocardial contraction without toxicity suggest the potential of GPL-DHA as a dietary supplement or ingredient for functional food, and possibly as a co-treatment with digitalis drugs in humans.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Bernard</LastName><ForeName>Monique</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Aix-Marseille Université, CNRS, CRMBM UMR 7339, F-13385 Marseille, France. monique.bernard@univ-amu.fr.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Maixent</LastName><ForeName>Jean-Michel</ForeName><Initials>JM</Initials><AffiliationInfo><Affiliation>EB2M-Protée, Université du Sud Toulon-Var, F-83957 La Garde, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Gerbi</LastName><ForeName>Alain</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>RDVC Produits Santé, F-76600 Le Havre, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Lan</LastName><ForeName>Carole</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>Aix-Marseille Université, CNRS, CRMBM UMR 7339, F-13385 Marseille, France. monique.bernard@univ-amu.fr.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Cozzone</LastName><ForeName>Patrick Jean</ForeName><Initials>PJ</Initials><AffiliationInfo><Affiliation>Aix-Marseille Université, CNRS, CRMBM UMR 7339, F-13385 Marseille, France. monique.bernard@univ-amu.fr.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Pieroni</LastName><ForeName>Gérard</ForeName><Initials>G</Initials><AffiliationInfo><Affiliation>Application Santé des Lipides, ASL, Bioparc de Vichy, F-03270 Hauterive, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Armand</LastName><ForeName>Martine</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Aix-Marseille Université, CNRS, CRMBM UMR 7339, F-13385 Marseille, France. monique.bernard@univ-amu.fr.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Coste</LastName><ForeName>Thierry Charles</ForeName><Initials>TC</Initials><AffiliationInfo><Affiliation>Novastell, F-27150 Etrépagny, France.</Affiliation></AffiliationInfo></Author></AuthorList><Language>ENG</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Food Funct</MedlineTA><NlmUniqueID>101549033</NlmUniqueID><ISSNLinking>2042-6496</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2015</Year><Month>12</Month><Day>15</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2015</Year><Month>12</Month><Day>15</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2015</Year><Month>12</Month><Day>15</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">26662260</ArticleId><ArticleId IdType="doi">10.1039/c5fo01300c</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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