First evidence for a Vibrio strain pathogenic to Mytilus edulis altering hemocyte immune capacities.
Identifieur interne : 000047 ( PubMed/Corpus ); précédent : 000046; suivant : 000048First evidence for a Vibrio strain pathogenic to Mytilus edulis altering hemocyte immune capacities.
Auteurs : Yosra Ben Cheikh ; Marie-Agnès Travers ; Benjamin Morga ; Yoann Godfrin ; Damien Rioult ; Frank Le FollSource :
- Developmental and comparative immunology [ 1879-0089 ] ; 2016.
English descriptors
- KwdEn :
- Animals, Cell Movement, Hemocytes (immunology), Hemocytes (microbiology), Immune Evasion, Immunity, Cellular, Immunity, Innate, Immunomodulation, Mytilus edulis (immunology), Oxidative Stress, Phagocytosis, Species Specificity, Vibrio (immunology), Vibrio (pathogenicity), Vibrio Infections (immunology), Virulence.
- MESH :
- immunology : Hemocytes, Mytilus edulis, Vibrio, Vibrio Infections.
- microbiology : Hemocytes.
- pathogenicity : Vibrio.
- Animals, Cell Movement, Immune Evasion, Immunity, Cellular, Immunity, Innate, Immunomodulation, Oxidative Stress, Phagocytosis, Species Specificity, Virulence.
Abstract
Bacterial isolates were obtained from mortality events affecting Mytilus edulis and reported by professionals in 2010-2013 or from mussel microflora. Experimental infections allowed the selection of two isolates affiliated to Vibrio splendidus/Vibrio hemicentroti type strains: a virulent 10/068 1T1 (76.6% and 90% mortalities in 24 h and 96 h) and an innocuous 12/056 M24T1 (0% and 23.3% in 24 h and 96 h). These two strains were GFP-tagged and validated for their growth characteristics and virulence as genuine models for exposure. Then, host cellular immune responses to the microbial invaders were assessed. In the presence of the virulent strain, hemocyte motility was instantaneously enhanced but markedly slowed down after 2 h exposure. By contrast, hemocyte velocity increased in the presence of the innocuous 12/056 M24T1. At the same time interval, 10/068 1T1 invaded hemocytes and was more rapidly internalized than the innocuous strain. Extracellular products (ECPs) prepared from 10/068 1T1 cultures significantly inhibited phagocytic activity while 12/056 M24T1 ECPs had no effect. Furthermore, the pathogenic strain and its ECPs inhibited oxidative burst unlike 12/056 M24T1 strain/ECPs which enhanced ROS production. Taken together, our results suggest that the mussel pathogen 10/068 1T1 may escape immune response by altering hemocytes functions.
DOI: 10.1016/j.dci.2015.12.014
PubMed: 26719026
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Electronic address: frank.lefoll@univ-lehavre.fr.</nlm:affiliation></affiliation></author></analytic><series><title level="j">Developmental and comparative immunology</title><idno type="eISSN">1879-0089</idno><imprint><date when="2016" type="published">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Cell Movement</term><term>Hemocytes (immunology)</term><term>Hemocytes (microbiology)</term><term>Immune Evasion</term><term>Immunity, Cellular</term><term>Immunity, Innate</term><term>Immunomodulation</term><term>Mytilus edulis (immunology)</term><term>Oxidative Stress</term><term>Phagocytosis</term><term>Species Specificity</term><term>Vibrio (immunology)</term><term>Vibrio (pathogenicity)</term><term>Vibrio Infections (immunology)</term><term>Virulence</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Hemocytes</term><term>Mytilus edulis</term><term>Vibrio</term><term>Vibrio Infections</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Hemocytes</term></keywords><keywords scheme="MESH" qualifier="pathogenicity" xml:lang="en"><term>Vibrio</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Cell Movement</term><term>Immune Evasion</term><term>Immunity, Cellular</term><term>Immunity, Innate</term><term>Immunomodulation</term><term>Oxidative Stress</term><term>Phagocytosis</term><term>Species Specificity</term><term>Virulence</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Bacterial isolates were obtained from mortality events affecting Mytilus edulis and reported by professionals in 2010-2013 or from mussel microflora. Experimental infections allowed the selection of two isolates affiliated to Vibrio splendidus/Vibrio hemicentroti type strains: a virulent 10/068 1T1 (76.6% and 90% mortalities in 24 h and 96 h) and an innocuous 12/056 M24T1 (0% and 23.3% in 24 h and 96 h). These two strains were GFP-tagged and validated for their growth characteristics and virulence as genuine models for exposure. Then, host cellular immune responses to the microbial invaders were assessed. In the presence of the virulent strain, hemocyte motility was instantaneously enhanced but markedly slowed down after 2 h exposure. By contrast, hemocyte velocity increased in the presence of the innocuous 12/056 M24T1. At the same time interval, 10/068 1T1 invaded hemocytes and was more rapidly internalized than the innocuous strain. Extracellular products (ECPs) prepared from 10/068 1T1 cultures significantly inhibited phagocytic activity while 12/056 M24T1 ECPs had no effect. Furthermore, the pathogenic strain and its ECPs inhibited oxidative burst unlike 12/056 M24T1 strain/ECPs which enhanced ROS production. Taken together, our results suggest that the mussel pathogen 10/068 1T1 may escape immune response by altering hemocytes functions.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">26719026</PMID><DateCreated><Year>2016</Year><Month>1</Month><Day>31</Day></DateCreated><DateCompleted><Year>2016</Year><Month>10</Month><Day>21</Day></DateCompleted><DateRevised><Year>2016</Year><Month>10</Month><Day>22</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1879-0089</ISSN><JournalIssue CitedMedium="Internet"><Volume>57</Volume><PubDate><Year>2016</Year><Month>Apr</Month></PubDate></JournalIssue><Title>Developmental and comparative immunology</Title><ISOAbbreviation>Dev. Comp. Immunol.</ISOAbbreviation></Journal><ArticleTitle>First evidence for a Vibrio strain pathogenic to Mytilus edulis altering hemocyte immune capacities.</ArticleTitle><Pagination><MedlinePgn>107-19</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.dci.2015.12.014</ELocationID><ELocationID EIdType="pii" ValidYN="Y">S0145-305X(15)30097-5</ELocationID><Abstract><AbstractText>Bacterial isolates were obtained from mortality events affecting Mytilus edulis and reported by professionals in 2010-2013 or from mussel microflora. Experimental infections allowed the selection of two isolates affiliated to Vibrio splendidus/Vibrio hemicentroti type strains: a virulent 10/068 1T1 (76.6% and 90% mortalities in 24 h and 96 h) and an innocuous 12/056 M24T1 (0% and 23.3% in 24 h and 96 h). These two strains were GFP-tagged and validated for their growth characteristics and virulence as genuine models for exposure. Then, host cellular immune responses to the microbial invaders were assessed. In the presence of the virulent strain, hemocyte motility was instantaneously enhanced but markedly slowed down after 2 h exposure. By contrast, hemocyte velocity increased in the presence of the innocuous 12/056 M24T1. At the same time interval, 10/068 1T1 invaded hemocytes and was more rapidly internalized than the innocuous strain. Extracellular products (ECPs) prepared from 10/068 1T1 cultures significantly inhibited phagocytic activity while 12/056 M24T1 ECPs had no effect. Furthermore, the pathogenic strain and its ECPs inhibited oxidative burst unlike 12/056 M24T1 strain/ECPs which enhanced ROS production. Taken together, our results suggest that the mussel pathogen 10/068 1T1 may escape immune response by altering hemocytes functions.</AbstractText><CopyrightInformation>Copyright © 2015 Elsevier Ltd. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Ben Cheikh</LastName><ForeName>Yosra</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>Laboratory of Ecotoxicology- Aquatic Environments, UMR-I 02, SEBIO, University of Le Havre, F-76063, Le Havre Cedex, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Travers</LastName><ForeName>Marie-Agnès</ForeName><Initials>MA</Initials><AffiliationInfo><Affiliation>Ifremer, SG2M-LGPMM, Laboratoire de Génétique et Pathologie des Mollusques Marins, Avenue de Mus de Loup, 17390, La Tremblade, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Morga</LastName><ForeName>Benjamin</ForeName><Initials>B</Initials><AffiliationInfo><Affiliation>Ifremer, SG2M-LGPMM, Laboratoire de Génétique et Pathologie des Mollusques Marins, Avenue de Mus de Loup, 17390, La Tremblade, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Godfrin</LastName><ForeName>Yoann</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>Ifremer, SG2M-LGPMM, Laboratoire de Génétique et Pathologie des Mollusques Marins, Avenue de Mus de Loup, 17390, La Tremblade, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Rioult</LastName><ForeName>Damien</ForeName><Initials>D</Initials><AffiliationInfo><Affiliation>Laboratory of Ecotoxicology- Aquatic Environments, UMR-I 02, SEBIO, University of Reims Champagne Ardenne, Campus Moulin de la House, F-51100, Reims, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Le Foll</LastName><ForeName>Frank</ForeName><Initials>F</Initials><AffiliationInfo><Affiliation>Laboratory of Ecotoxicology- Aquatic Environments, UMR-I 02, SEBIO, University of Le Havre, F-76063, Le Havre Cedex, France. Electronic address: frank.lefoll@univ-lehavre.fr.</Affiliation></AffiliationInfo></Author></AuthorList><Language>ENG</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2015</Year><Month>Dec</Month><Day>21</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Dev Comp Immunol</MedlineTA><NlmUniqueID>7708205</NlmUniqueID><ISSNLinking>0145-305X</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002465" MajorTopicYN="N">Cell Movement</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006434" MajorTopicYN="N">Hemocytes</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D057131" MajorTopicYN="N">Immune Evasion</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D007111" MajorTopicYN="N">Immunity, Cellular</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D007113" MajorTopicYN="N">Immunity, Innate</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D056747" MajorTopicYN="N">Immunomodulation</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D049880" MajorTopicYN="N">Mytilus edulis</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018384" MajorTopicYN="N">Oxidative Stress</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010587" MajorTopicYN="N">Phagocytosis</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D013045" MajorTopicYN="N">Species Specificity</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014733" MajorTopicYN="N">Vibrio</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName><QualifierName UI="Q000472" MajorTopicYN="N">pathogenicity</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014735" MajorTopicYN="N">Vibrio Infections</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014774" MajorTopicYN="N">Virulence</DescriptorName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Bacterial infections</Keyword><Keyword MajorTopicYN="N">Cell-mediated immune response</Keyword><Keyword MajorTopicYN="N">Green fluorescent protein</Keyword><Keyword MajorTopicYN="N">Innate immunity</Keyword><Keyword MajorTopicYN="N">Molluscs</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2015</Year><Month>11</Month><Day>18</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2015</Year><Month>12</Month><Day>17</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2015</Year><Month>12</Month><Day>17</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2016</Year><Month>1</Month><Day>1</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2016</Year><Month>1</Month><Day>1</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2016</Year><Month>10</Month><Day>22</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">26719026</ArticleId><ArticleId IdType="pii">S0145-305X(15)30097-5</ArticleId><ArticleId IdType="doi">10.1016/j.dci.2015.12.014</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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