Differential protein expression in the estuarine copepod Eurytemora affinis after diuron and alkylphenol exposures.
Identifieur interne : 000028 ( PubMed/Corpus ); précédent : 000027; suivant : 000029Differential protein expression in the estuarine copepod Eurytemora affinis after diuron and alkylphenol exposures.
Auteurs : Céline Boulangé-Lecomte ; Béatrice Rocher ; Kévin Cailleaud ; Pascal Cosette ; Eléna Legrand ; David Devreker ; Hélène Budzinski ; Sami Souissi ; Joëlle Forget-LeraySource :
- Environmental toxicology and chemistry [ 1552-8618 ] ; 2016.
Abstract
Proteomics was used in the calanoid copepod Eurytemora affinis for screening of protein expression modifications induced by organic contaminants. The copepods were exposed in a continuous flow-through system for 86 h to environmentally relevant concentrations of contaminants representative of the pollution in the Seine Estuary (Haute-Normandie, France; diuron, 500 ng L(-1) ; alkylphenol mixture, 1000 ng L(-1) ). Proteome analysis of whole-body copepod extracts by 2-dimensional gel electrophoresis revealed that the contaminants induced modifications in protein expression, with the highest quantitative variations occurring after diuron exposure. Specifically, 88 and 41 proteins were differentially expressed after diuron and alkylphenol treatments, respectively. After mass spectrometry analysis, 51 (diuron exposure) and 15 (alkylphenol exposure) proteins were identified. The identified proteins were potentially related to energy metabolism, cell growth, nervous signal conductivity, excitotoxicity, oxidative stress response, and antioxidant defense. The data suggest a massive general disturbance of physiological functions of E. affinis after diuron exposure, whereas alkylphenols induced an alteration of a few targeted physiological functions. The protein expression signatures identified after contaminant exposure deserve further investigation in terms of the development of novel potential biomarkers for water quality assessment. Environ Toxicol Chem 2016;35:1860-1871. © 2015 SETAC.
DOI: 10.1002/etc.3343
PubMed: 26677818
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last="Souissi">Sami Souissi</name><affiliation><nlm:affiliation>UMR CNRS 8187 Laboratoire d'Océanologie et de Géosciences (LOG), Wimereux Marine Station, Lille 1 University, Wimereux, France.</nlm:affiliation></affiliation></author><author><name sortKey="Forget Leray, Joelle" sort="Forget Leray, Joelle" uniqKey="Forget Leray J" first="Joëlle" last="Forget-Leray">Joëlle Forget-Leray</name><affiliation><nlm:affiliation>UMR-I 02 Laboratoire Stress Environnementaux et BIOsurveillance des milieux aquatiques (SEBIO), FR CNRS 3730 SCALE, University of Le Havre, Normandy University, Le Havre, France.</nlm:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2016">2016</date><idno type="RBID">pubmed:26677818</idno><idno type="pmid">26677818</idno><idno type="doi">10.1002/etc.3343</idno><idno type="wicri:Area/PubMed/Corpus">000028</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Differential protein expression in the estuarine copepod Eurytemora affinis after diuron and alkylphenol exposures.</title><author><name sortKey="Boulange Lecomte, Celine" sort="Boulange Lecomte, Celine" uniqKey="Boulange Lecomte C" first="Céline" last="Boulangé-Lecomte">Céline Boulangé-Lecomte</name><affiliation><nlm:affiliation>UMR-I 02 Laboratoire Stress Environnementaux et BIOsurveillance des milieux aquatiques (SEBIO), FR CNRS 3730 SCALE, University of Le Havre, Normandy University, Le Havre, France.</nlm:affiliation></affiliation></author><author><name sortKey="Rocher, Beatrice" sort="Rocher, Beatrice" uniqKey="Rocher B" first="Béatrice" last="Rocher">Béatrice Rocher</name><affiliation><nlm:affiliation>UMR-I 02 Laboratoire Stress Environnementaux et BIOsurveillance des milieux aquatiques (SEBIO), FR CNRS 3730 SCALE, University of Le Havre, Normandy University, Le Havre, France.</nlm:affiliation></affiliation></author><author><name sortKey="Cailleaud, Kevin" sort="Cailleaud, Kevin" uniqKey="Cailleaud K" first="Kévin" last="Cailleaud">Kévin Cailleaud</name><affiliation><nlm:affiliation>UMR-I 02 Laboratoire Stress Environnementaux et BIOsurveillance des milieux aquatiques (SEBIO), FR CNRS 3730 SCALE, University of Le Havre, Normandy University, Le Havre, France.</nlm:affiliation></affiliation></author><author><name sortKey="Cosette, Pascal" sort="Cosette, Pascal" uniqKey="Cosette P" first="Pascal" last="Cosette">Pascal Cosette</name><affiliation><nlm:affiliation>UMR CNRS 6270, Laboratoire Polymères-Biopolymères-Surfaces (LPBS), University of Rouen, Normandy University, Mont-Saint-Aignan, France.</nlm:affiliation></affiliation></author><author><name sortKey="Legrand, Elena" sort="Legrand, Elena" uniqKey="Legrand E" first="Eléna" last="Legrand">Eléna Legrand</name><affiliation><nlm:affiliation>UMR-I 02 Laboratoire Stress Environnementaux et BIOsurveillance des milieux aquatiques (SEBIO), FR CNRS 3730 SCALE, University of Le Havre, Normandy University, Le Havre, France.</nlm:affiliation></affiliation></author><author><name sortKey="Devreker, David" sort="Devreker, David" uniqKey="Devreker D" first="David" last="Devreker">David Devreker</name><affiliation><nlm:affiliation>UMR CNRS 8187 Laboratoire d'Océanologie et de Géosciences (LOG), Wimereux Marine Station, Lille 1 University, Wimereux, France.</nlm:affiliation></affiliation></author><author><name sortKey="Budzinski, Helene" sort="Budzinski, Helene" uniqKey="Budzinski H" first="Hélène" last="Budzinski">Hélène Budzinski</name><affiliation><nlm:affiliation>UMR CNRS 5805, Environnements et Paléoenvironnements Océaniques et Continentaux (EPOC), Laboratoire de Physico- et Toxico-Chimie de l'environnement (LPTC), Bordeaux University, Talence, France.</nlm:affiliation></affiliation></author><author><name sortKey="Souissi, Sami" sort="Souissi, Sami" uniqKey="Souissi S" first="Sami" last="Souissi">Sami Souissi</name><affiliation><nlm:affiliation>UMR CNRS 8187 Laboratoire d'Océanologie et de Géosciences (LOG), Wimereux Marine Station, Lille 1 University, Wimereux, France.</nlm:affiliation></affiliation></author><author><name sortKey="Forget Leray, Joelle" sort="Forget Leray, Joelle" uniqKey="Forget Leray J" first="Joëlle" last="Forget-Leray">Joëlle Forget-Leray</name><affiliation><nlm:affiliation>UMR-I 02 Laboratoire Stress Environnementaux et BIOsurveillance des milieux aquatiques (SEBIO), FR CNRS 3730 SCALE, University of Le Havre, Normandy University, Le Havre, France.</nlm:affiliation></affiliation></author></analytic><series><title level="j">Environmental toxicology and chemistry</title><idno type="eISSN">1552-8618</idno><imprint><date when="2016" type="published">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Proteomics was used in the calanoid copepod Eurytemora affinis for screening of protein expression modifications induced by organic contaminants. The copepods were exposed in a continuous flow-through system for 86 h to environmentally relevant concentrations of contaminants representative of the pollution in the Seine Estuary (Haute-Normandie, France; diuron, 500 ng L(-1) ; alkylphenol mixture, 1000 ng L(-1) ). Proteome analysis of whole-body copepod extracts by 2-dimensional gel electrophoresis revealed that the contaminants induced modifications in protein expression, with the highest quantitative variations occurring after diuron exposure. Specifically, 88 and 41 proteins were differentially expressed after diuron and alkylphenol treatments, respectively. After mass spectrometry analysis, 51 (diuron exposure) and 15 (alkylphenol exposure) proteins were identified. The identified proteins were potentially related to energy metabolism, cell growth, nervous signal conductivity, excitotoxicity, oxidative stress response, and antioxidant defense. The data suggest a massive general disturbance of physiological functions of E. affinis after diuron exposure, whereas alkylphenols induced an alteration of a few targeted physiological functions. The protein expression signatures identified after contaminant exposure deserve further investigation in terms of the development of novel potential biomarkers for water quality assessment. Environ Toxicol Chem 2016;35:1860-1871. © 2015 SETAC.</div></front></TEI><pubmed><MedlineCitation Status="In-Data-Review" Owner="NLM"><PMID Version="1">26677818</PMID><DateCreated><Year>2016</Year><Month>6</Month><Day>23</Day></DateCreated><DateRevised><Year>2016</Year><Month>10</Month><Day>18</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1552-8618</ISSN><JournalIssue CitedMedium="Internet"><Volume>35</Volume><Issue>7</Issue><PubDate><Year>2016</Year><Month>Jul</Month></PubDate></JournalIssue><Title>Environmental toxicology and chemistry</Title><ISOAbbreviation>Environ. Toxicol. Chem.</ISOAbbreviation></Journal><ArticleTitle>Differential protein expression in the estuarine copepod Eurytemora affinis after diuron and alkylphenol exposures.</ArticleTitle><Pagination><MedlinePgn>1860-71</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1002/etc.3343</ELocationID><Abstract><AbstractText>Proteomics was used in the calanoid copepod Eurytemora affinis for screening of protein expression modifications induced by organic contaminants. The copepods were exposed in a continuous flow-through system for 86 h to environmentally relevant concentrations of contaminants representative of the pollution in the Seine Estuary (Haute-Normandie, France; diuron, 500 ng L(-1) ; alkylphenol mixture, 1000 ng L(-1) ). Proteome analysis of whole-body copepod extracts by 2-dimensional gel electrophoresis revealed that the contaminants induced modifications in protein expression, with the highest quantitative variations occurring after diuron exposure. Specifically, 88 and 41 proteins were differentially expressed after diuron and alkylphenol treatments, respectively. After mass spectrometry analysis, 51 (diuron exposure) and 15 (alkylphenol exposure) proteins were identified. The identified proteins were potentially related to energy metabolism, cell growth, nervous signal conductivity, excitotoxicity, oxidative stress response, and antioxidant defense. The data suggest a massive general disturbance of physiological functions of E. affinis after diuron exposure, whereas alkylphenols induced an alteration of a few targeted physiological functions. The protein expression signatures identified after contaminant exposure deserve further investigation in terms of the development of novel potential biomarkers for water quality assessment. Environ Toxicol Chem 2016;35:1860-1871. © 2015 SETAC.</AbstractText><CopyrightInformation>© 2015 SETAC.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Boulangé-Lecomte</LastName><ForeName>Céline</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>UMR-I 02 Laboratoire Stress Environnementaux et BIOsurveillance des milieux aquatiques (SEBIO), FR CNRS 3730 SCALE, University of Le Havre, Normandy University, Le Havre, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Rocher</LastName><ForeName>Béatrice</ForeName><Initials>B</Initials><AffiliationInfo><Affiliation>UMR-I 02 Laboratoire Stress Environnementaux et BIOsurveillance des milieux aquatiques (SEBIO), FR CNRS 3730 SCALE, University of Le Havre, Normandy University, Le Havre, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Cailleaud</LastName><ForeName>Kévin</ForeName><Initials>K</Initials><AffiliationInfo><Affiliation>UMR-I 02 Laboratoire Stress Environnementaux et BIOsurveillance des milieux aquatiques (SEBIO), FR CNRS 3730 SCALE, University of Le Havre, Normandy University, Le Havre, France.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>UMR CNRS 5805, Environnements et Paléoenvironnements Océaniques et Continentaux (EPOC), Laboratoire de Physico- et Toxico-Chimie de l'environnement (LPTC), Bordeaux University, Talence, France.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>UMR CNRS 8187 Laboratoire d'Océanologie et de Géosciences (LOG), Wimereux Marine Station, Lille 1 University, Wimereux, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Cosette</LastName><ForeName>Pascal</ForeName><Initials>P</Initials><AffiliationInfo><Affiliation>UMR CNRS 6270, Laboratoire Polymères-Biopolymères-Surfaces (LPBS), University of Rouen, Normandy University, Mont-Saint-Aignan, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Legrand</LastName><ForeName>Eléna</ForeName><Initials>E</Initials><AffiliationInfo><Affiliation>UMR-I 02 Laboratoire Stress Environnementaux et BIOsurveillance des milieux aquatiques (SEBIO), FR CNRS 3730 SCALE, University of Le Havre, Normandy University, Le Havre, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Devreker</LastName><ForeName>David</ForeName><Initials>D</Initials><AffiliationInfo><Affiliation>UMR CNRS 8187 Laboratoire d'Océanologie et de Géosciences (LOG), Wimereux Marine Station, Lille 1 University, Wimereux, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Budzinski</LastName><ForeName>Hélène</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>UMR CNRS 5805, Environnements et Paléoenvironnements Océaniques et Continentaux (EPOC), Laboratoire de Physico- et Toxico-Chimie de l'environnement (LPTC), Bordeaux University, Talence, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Souissi</LastName><ForeName>Sami</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>UMR CNRS 8187 Laboratoire d'Océanologie et de Géosciences (LOG), Wimereux Marine Station, Lille 1 University, Wimereux, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Forget-Leray</LastName><ForeName>Joëlle</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>UMR-I 02 Laboratoire Stress Environnementaux et BIOsurveillance des milieux aquatiques (SEBIO), FR CNRS 3730 SCALE, University of Le Havre, Normandy University, Le Havre, France.</Affiliation></AffiliationInfo></Author></AuthorList><Language>ENG</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2016</Year><Month>May</Month><Day>03</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Environ Toxicol Chem</MedlineTA><NlmUniqueID>8308958</NlmUniqueID><ISSNLinking>0730-7268</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">2D electrophoresis</Keyword><Keyword MajorTopicYN="N">Biomarkers</Keyword><Keyword MajorTopicYN="N">Calanoid</Keyword><Keyword MajorTopicYN="N">Estuary</Keyword><Keyword MajorTopicYN="N">Organic contaminants</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2015</Year><Month>8</Month><Day>24</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2015</Year><Month>10</Month><Day>6</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2015</Year><Month>12</Month><Day>13</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2015</Year><Month>12</Month><Day>19</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate 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