Genetic and immunological characterisation of a multixenobiotic resistance system in the turbot (Scophthalmus maximus).
Identifieur interne : 000351 ( PubMed/Checkpoint ); précédent : 000350; suivant : 000352Genetic and immunological characterisation of a multixenobiotic resistance system in the turbot (Scophthalmus maximus).
Auteurs : Renaud Tutundjian [France] ; J. Cachot ; F. Leboulenger ; C. MinierSource :
- Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology [ 1096-4959 ] ; 2002.
English descriptors
- KwdEn :
- Amino Acid Sequence, Animals, Base Sequence, Cloning, Molecular, DNA, Complementary (genetics), Drug Resistance, Multiple (genetics), Drug Resistance, Multiple (immunology), Fish Proteins (analysis), Fish Proteins (chemistry), Fish Proteins (genetics), Flatfishes (genetics), Flatfishes (immunology), Gene Expression Profiling, Genes, MDR (genetics), Humans, Molecular Sequence Data, Molecular Weight, Organ Specificity, RNA, Messenger (genetics), RNA, Messenger (metabolism), Reverse Transcriptase Polymerase Chain Reaction, Sequence Analysis, DNA, Xenobiotics (pharmacology).
- MESH :
- chemical , analysis : Fish Proteins.
- chemical , chemistry : Fish Proteins.
- chemical , genetics : DNA, Complementary, Fish Proteins, RNA, Messenger.
- genetics : Drug Resistance, Multiple, Flatfishes, Genes, MDR.
- immunology : Drug Resistance, Multiple, Flatfishes.
- chemical , metabolism : RNA, Messenger.
- chemical , pharmacology : Xenobiotics.
- Amino Acid Sequence, Animals, Base Sequence, Cloning, Molecular, Gene Expression Profiling, Humans, Molecular Sequence Data, Molecular Weight, Organ Specificity, Reverse Transcriptase Polymerase Chain Reaction, Sequence Analysis, DNA.
Abstract
Pleiotropic resistance driven by transport proteins constitutes a very ubiquitous protection mechanism against natural or synthetic toxic compounds. The multidrug (MDR) or multixenobiotic (MXR) system has been identified in many different species, and may be used as a biomarker for pollution assessment. Here we report the existence of a gene encoding a MXR-related protein in a benthic fish species, the turbot Scophthalmus maximus, and its constitutive expression in several tissues. A 433bp cDNA fragment has been cloned by RT-PCR. The deduced amino-acid sequence shares close to 80% homology with class I or class II mammalian MDR proteins. This cDNA corresponds to a major mRNA of 5.6 kb and encodes a protein having an apparent molecular weight of 83 kDa. Constitutive expression levels assessed by semi-quantitative RT-PCR and Western blot, revealed that the kidney and the brain, and to a lesser extent, the heart, gills and intestine, are the organs which contain the highest amount of both MXR mRNAs or proteins. This tissue specific expression suggests a role for the identified mechanism in protection against endogenous or exogenous toxic compounds.
PubMed: 12031473
Affiliations:
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Cachot</name></author><author><name sortKey="Leboulenger, F" sort="Leboulenger, F" uniqKey="Leboulenger F" first="F" last="Leboulenger">F. Leboulenger</name></author><author><name sortKey="Minier, C" sort="Minier, C" uniqKey="Minier C" first="C" last="Minier">C. 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The multidrug (MDR) or multixenobiotic (MXR) system has been identified in many different species, and may be used as a biomarker for pollution assessment. Here we report the existence of a gene encoding a MXR-related protein in a benthic fish species, the turbot Scophthalmus maximus, and its constitutive expression in several tissues. A 433bp cDNA fragment has been cloned by RT-PCR. The deduced amino-acid sequence shares close to 80% homology with class I or class II mammalian MDR proteins. This cDNA corresponds to a major mRNA of 5.6 kb and encodes a protein having an apparent molecular weight of 83 kDa. Constitutive expression levels assessed by semi-quantitative RT-PCR and Western blot, revealed that the kidney and the brain, and to a lesser extent, the heart, gills and intestine, are the organs which contain the highest amount of both MXR mRNAs or proteins. This tissue specific expression suggests a role for the identified mechanism in protection against endogenous or exogenous toxic compounds.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">12031473</PMID><DateCreated><Year>2002</Year><Month>5</Month><Day>28</Day></DateCreated><DateCompleted><Year>2003</Year><Month>01</Month><Day>03</Day></DateCompleted><DateRevised><Year>2006</Year><Month>11</Month><Day>15</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">1096-4959</ISSN><JournalIssue CitedMedium="Print"><Volume>132</Volume><Issue>2</Issue><PubDate><Year>2002</Year><Month>Jun</Month></PubDate></JournalIssue><Title>Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology</Title><ISOAbbreviation>Comp. Biochem. Physiol. B, Biochem. Mol. 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This cDNA corresponds to a major mRNA of 5.6 kb and encodes a protein having an apparent molecular weight of 83 kDa. Constitutive expression levels assessed by semi-quantitative RT-PCR and Western blot, revealed that the kidney and the brain, and to a lesser extent, the heart, gills and intestine, are the organs which contain the highest amount of both MXR mRNAs or proteins. 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PubStatus="medline"><Year>2003</Year><Month>1</Month><Day>4</Day><Hour>4</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2002</Year><Month>5</Month><Day>29</Day><Hour>10</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">12031473</ArticleId><ArticleId IdType="pii">S1096495902000581</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>France</li></country><region><li>Haute-Normandie</li><li>Région Normandie</li></region><settlement><li>Le Havre</li></settlement><orgName><li>Université du Havre</li></orgName></list><tree><noCountry><name sortKey="Cachot, J" sort="Cachot, J" uniqKey="Cachot J" first="J" last="Cachot">J. Cachot</name><name sortKey="Leboulenger, F" sort="Leboulenger, F" uniqKey="Leboulenger F" first="F" last="Leboulenger">F. Leboulenger</name><name sortKey="Minier, C" sort="Minier, C" uniqKey="Minier C" first="C" last="Minier">C. Minier</name></noCountry><country name="France"><region name="Région Normandie"><name sortKey="Tutundjian, Renaud" sort="Tutundjian, Renaud" uniqKey="Tutundjian R" first="Renaud" last="Tutundjian">Renaud Tutundjian</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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