Evidence of genotoxicity related to high PAH content of sediments in the upper part of the Seine estuary (Normandy, France).
Identifieur interne : 000290 ( PubMed/Checkpoint ); précédent : 000289; suivant : 000291Evidence of genotoxicity related to high PAH content of sediments in the upper part of the Seine estuary (Normandy, France).
Auteurs : J. Cachot [France] ; O. Geffard ; S. Augagneur ; S. Lacroix ; K. Le Menach ; L. Peluhet ; J. Couteau ; X. Denier ; M H Devier ; D. Pottier ; H. BudzinskiSource :
- Aquatic toxicology (Amsterdam, Netherlands) [ 0166-445X ] ; 2006.
Descripteurs français
- Wicri :
- geographic : France.
English descriptors
- KwdEn :
- Animals, Crassostrea (drug effects), Crassostrea (genetics), Embryo, Nonmammalian (drug effects), France, Geologic Sediments (analysis), Hydrocarbons, Chlorinated (analysis), Inhibitory Concentration 50, Metals, Heavy (analysis), Mutagenicity Tests (methods), Polycyclic Hydrocarbons, Aromatic (analysis), Polycyclic Hydrocarbons, Aromatic (toxicity), Rivers, Water Pollutants, Chemical (toxicity).
- MESH :
- chemical , analysis : Hydrocarbons, Chlorinated, Metals, Heavy, Polycyclic Hydrocarbons, Aromatic.
- chemical , toxicity : Polycyclic Hydrocarbons, Aromatic, Water Pollutants, Chemical.
- geographic : France.
- analysis : Geologic Sediments.
- drug effects : Crassostrea, Embryo, Nonmammalian.
- genetics : Crassostrea.
- methods : Mutagenicity Tests.
- Animals, Inhibitory Concentration 50, Rivers.
Abstract
In order to characterize the genotoxicity in the Seine estuary and Seine bay, chemical and toxicological analyses were performed on 17 sediments collected in June 2001 and June 2003. Many potent mutagenic and/or carcinogenic compounds - including polycyclic aromatic hydrocarbons (PAH), polychlorinated biphenyls (PCB) and metals - were detected. Those compounds were found to be at relatively high concentrations in the upper part of the Seine estuary but were barely detectable at sites outside the plume from the Seine. The levels of pollution did not vary significantly between the two sampling periods, except that PAH concentrations in sediments collected at Oissel and Le Havre showed a marked increase in June 2003. The toxicity of organic extracts from sediments was evaluated by both embryotoxicity and in vitro genotoxicity (SOS Chromotest) assays. Organic extracts from sediments taken from the Seine estuary appeared significantly more embryotoxic than those from the Seine bay. In addition, the sediment extracts from the upper part of the Seine estuary exhibited higher genotoxicity than those from the lower part, and no genotoxicity was reported for sediments from the Seine bay. The genotoxic activity was detected only after adding an S9 microsomal fraction, suggesting the preponderant involvement of pro-genotoxic organic compounds. In addition, SOS Chromotest responses obtained with purified organic fractions revealed that PAH and, to a lesser extent, unknown polar organic compounds were probably responsible for this genotoxicity. Altogether, these results suggest that sediments from the upper Seine estuary are genotoxic and embryotoxic, and therefore, could be potentially hazardous for species living or feeding in the area.
DOI: 10.1016/j.aquatox.2006.06.014
PubMed: 16887205
Affiliations:
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Jerome.Cachot@univ-lehavre.fr</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Laboratory of Ecotoxicology (LEMA), UPRES EA-3222, IFRMP 23, University of Le Havre, 25 rue Philippe Lebon, B.P. 540, 76058 Le Havre Cedex</wicri:regionArea><placeName><region type="region" nuts="2">Région Normandie</region><region type="old region" nuts="2">Haute-Normandie</region><settlement type="city">Le Havre</settlement></placeName></affiliation></author><author><name sortKey="Geffard, O" sort="Geffard, O" uniqKey="Geffard O" first="O" last="Geffard">O. Geffard</name></author><author><name sortKey="Augagneur, S" sort="Augagneur, S" uniqKey="Augagneur S" first="S" last="Augagneur">S. Augagneur</name></author><author><name sortKey="Lacroix, S" sort="Lacroix, S" uniqKey="Lacroix S" first="S" last="Lacroix">S. Lacroix</name></author><author><name sortKey="Le Menach, K" sort="Le Menach, K" uniqKey="Le Menach K" first="K" last="Le Menach">K. 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Jerome.Cachot@univ-lehavre.fr</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Laboratory of Ecotoxicology (LEMA), UPRES EA-3222, IFRMP 23, University of Le Havre, 25 rue Philippe Lebon, B.P. 540, 76058 Le Havre Cedex</wicri:regionArea><placeName><region type="region" nuts="2">Région Normandie</region><region type="old region" nuts="2">Haute-Normandie</region><settlement type="city">Le Havre</settlement></placeName></affiliation></author><author><name sortKey="Geffard, O" sort="Geffard, O" uniqKey="Geffard O" first="O" last="Geffard">O. Geffard</name></author><author><name sortKey="Augagneur, S" sort="Augagneur, S" uniqKey="Augagneur S" first="S" last="Augagneur">S. Augagneur</name></author><author><name sortKey="Lacroix, S" sort="Lacroix, S" uniqKey="Lacroix S" first="S" last="Lacroix">S. Lacroix</name></author><author><name sortKey="Le Menach, K" sort="Le Menach, K" uniqKey="Le Menach K" first="K" last="Le Menach">K. Le Menach</name></author><author><name sortKey="Peluhet, L" sort="Peluhet, L" uniqKey="Peluhet L" first="L" last="Peluhet">L. Peluhet</name></author><author><name sortKey="Couteau, J" sort="Couteau, J" uniqKey="Couteau J" first="J" last="Couteau">J. Couteau</name></author><author><name sortKey="Denier, X" sort="Denier, X" uniqKey="Denier X" first="X" last="Denier">X. Denier</name></author><author><name sortKey="Devier, M H" sort="Devier, M H" uniqKey="Devier M" first="M H" last="Devier">M H Devier</name></author><author><name sortKey="Pottier, D" sort="Pottier, D" uniqKey="Pottier D" first="D" last="Pottier">D. Pottier</name></author><author><name sortKey="Budzinski, H" sort="Budzinski, H" uniqKey="Budzinski H" first="H" last="Budzinski">H. Budzinski</name></author></analytic><series><title level="j">Aquatic toxicology (Amsterdam, Netherlands)</title><idno type="ISSN">0166-445X</idno><imprint><date when="2006" type="published">2006</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Crassostrea (drug effects)</term><term>Crassostrea (genetics)</term><term>Embryo, Nonmammalian (drug effects)</term><term>France</term><term>Geologic Sediments (analysis)</term><term>Hydrocarbons, Chlorinated (analysis)</term><term>Inhibitory Concentration 50</term><term>Metals, Heavy (analysis)</term><term>Mutagenicity Tests (methods)</term><term>Polycyclic Hydrocarbons, Aromatic (analysis)</term><term>Polycyclic Hydrocarbons, Aromatic (toxicity)</term><term>Rivers</term><term>Water Pollutants, Chemical (toxicity)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Hydrocarbons, Chlorinated</term><term>Metals, Heavy</term><term>Polycyclic Hydrocarbons, Aromatic</term></keywords><keywords scheme="MESH" type="chemical" qualifier="toxicity" xml:lang="en"><term>Polycyclic Hydrocarbons, Aromatic</term><term>Water Pollutants, Chemical</term></keywords><keywords scheme="MESH" type="geographic" xml:lang="en"><term>France</term></keywords><keywords scheme="MESH" qualifier="analysis" xml:lang="en"><term>Geologic Sediments</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Crassostrea</term><term>Embryo, Nonmammalian</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Crassostrea</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Mutagenicity Tests</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Inhibitory Concentration 50</term><term>Rivers</term></keywords><keywords scheme="Wicri" type="geographic" xml:lang="fr"><term>France</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">In order to characterize the genotoxicity in the Seine estuary and Seine bay, chemical and toxicological analyses were performed on 17 sediments collected in June 2001 and June 2003. Many potent mutagenic and/or carcinogenic compounds - including polycyclic aromatic hydrocarbons (PAH), polychlorinated biphenyls (PCB) and metals - were detected. Those compounds were found to be at relatively high concentrations in the upper part of the Seine estuary but were barely detectable at sites outside the plume from the Seine. The levels of pollution did not vary significantly between the two sampling periods, except that PAH concentrations in sediments collected at Oissel and Le Havre showed a marked increase in June 2003. The toxicity of organic extracts from sediments was evaluated by both embryotoxicity and in vitro genotoxicity (SOS Chromotest) assays. Organic extracts from sediments taken from the Seine estuary appeared significantly more embryotoxic than those from the Seine bay. In addition, the sediment extracts from the upper part of the Seine estuary exhibited higher genotoxicity than those from the lower part, and no genotoxicity was reported for sediments from the Seine bay. The genotoxic activity was detected only after adding an S9 microsomal fraction, suggesting the preponderant involvement of pro-genotoxic organic compounds. In addition, SOS Chromotest responses obtained with purified organic fractions revealed that PAH and, to a lesser extent, unknown polar organic compounds were probably responsible for this genotoxicity. Altogether, these results suggest that sediments from the upper Seine estuary are genotoxic and embryotoxic, and therefore, could be potentially hazardous for species living or feeding in the area.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">16887205</PMID><DateCreated><Year>2006</Year><Month>8</Month><Day>28</Day></DateCreated><DateCompleted><Year>2007</Year><Month>11</Month><Day>28</Day></DateCompleted><DateRevised><Year>2006</Year><Month>8</Month><Day>28</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Print">0166-445X</ISSN><JournalIssue CitedMedium="Print"><Volume>79</Volume><Issue>3</Issue><PubDate><Year>2006</Year><Month>Sep</Month><Day>12</Day></PubDate></JournalIssue><Title>Aquatic toxicology (Amsterdam, Netherlands)</Title><ISOAbbreviation>Aquat. Toxicol.</ISOAbbreviation></Journal><ArticleTitle>Evidence of genotoxicity related to high PAH content of sediments in the upper part of the Seine estuary (Normandy, France).</ArticleTitle><Pagination><MedlinePgn>257-67</MedlinePgn></Pagination><Abstract><AbstractText>In order to characterize the genotoxicity in the Seine estuary and Seine bay, chemical and toxicological analyses were performed on 17 sediments collected in June 2001 and June 2003. Many potent mutagenic and/or carcinogenic compounds - including polycyclic aromatic hydrocarbons (PAH), polychlorinated biphenyls (PCB) and metals - were detected. Those compounds were found to be at relatively high concentrations in the upper part of the Seine estuary but were barely detectable at sites outside the plume from the Seine. The levels of pollution did not vary significantly between the two sampling periods, except that PAH concentrations in sediments collected at Oissel and Le Havre showed a marked increase in June 2003. The toxicity of organic extracts from sediments was evaluated by both embryotoxicity and in vitro genotoxicity (SOS Chromotest) assays. Organic extracts from sediments taken from the Seine estuary appeared significantly more embryotoxic than those from the Seine bay. In addition, the sediment extracts from the upper part of the Seine estuary exhibited higher genotoxicity than those from the lower part, and no genotoxicity was reported for sediments from the Seine bay. The genotoxic activity was detected only after adding an S9 microsomal fraction, suggesting the preponderant involvement of pro-genotoxic organic compounds. In addition, SOS Chromotest responses obtained with purified organic fractions revealed that PAH and, to a lesser extent, unknown polar organic compounds were probably responsible for this genotoxicity. Altogether, these results suggest that sediments from the upper Seine estuary are genotoxic and embryotoxic, and therefore, could be potentially hazardous for species living or feeding in the area.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Cachot</LastName><ForeName>J</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Laboratory of Ecotoxicology (LEMA), UPRES EA-3222, IFRMP 23, University of Le Havre, 25 rue Philippe Lebon, B.P. 540, 76058 Le Havre Cedex, France. Jerome.Cachot@univ-lehavre.fr</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Geffard</LastName><ForeName>O</ForeName><Initials>O</Initials></Author><Author ValidYN="Y"><LastName>Augagneur</LastName><ForeName>S</ForeName><Initials>S</Initials></Author><Author ValidYN="Y"><LastName>Lacroix</LastName><ForeName>S</ForeName><Initials>S</Initials></Author><Author ValidYN="Y"><LastName>Le Menach</LastName><ForeName>K</ForeName><Initials>K</Initials></Author><Author ValidYN="Y"><LastName>Peluhet</LastName><ForeName>L</ForeName><Initials>L</Initials></Author><Author ValidYN="Y"><LastName>Couteau</LastName><ForeName>J</ForeName><Initials>J</Initials></Author><Author ValidYN="Y"><LastName>Denier</LastName><ForeName>X</ForeName><Initials>X</Initials></Author><Author ValidYN="Y"><LastName>Devier</LastName><ForeName>M H</ForeName><Initials>MH</Initials></Author><Author ValidYN="Y"><LastName>Pottier</LastName><ForeName>D</ForeName><Initials>D</Initials></Author><Author ValidYN="Y"><LastName>Budzinski</LastName><ForeName>H</ForeName><Initials>H</Initials></Author></AuthorList><Language>ENG</Language><PublicationTypeList><PublicationType UI="D003160">Comparative Study</PublicationType><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2006</Year><Month>Aug</Month><Day>02</Day></ArticleDate></Article><MedlineJournalInfo><Country>Netherlands</Country><MedlineTA>Aquat Toxicol</MedlineTA><NlmUniqueID>8500246</NlmUniqueID><ISSNLinking>0166-445X</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D006843">Hydrocarbons, Chlorinated</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D019216">Metals, Heavy</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D011084">Polycyclic Hydrocarbons, Aromatic</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D014874">Water Pollutants, Chemical</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D049896" MajorTopicYN="N">Crassostrea</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="Y">drug effects</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004625" MajorTopicYN="N">Embryo, Nonmammalian</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005602" MajorTopicYN="N" Type="Geographic">France</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D019015" MajorTopicYN="N">Geologic Sediments</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="Y">analysis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006843" MajorTopicYN="N">Hydrocarbons, Chlorinated</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="N">analysis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D020128" MajorTopicYN="N">Inhibitory Concentration 50</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D019216" MajorTopicYN="N">Metals, Heavy</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="N">analysis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D009152" MajorTopicYN="N">Mutagenicity Tests</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="N">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011084" MajorTopicYN="N">Polycyclic Hydrocarbons, Aromatic</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="N">analysis</QualifierName><QualifierName UI="Q000633" MajorTopicYN="Y">toxicity</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D045483" MajorTopicYN="N">Rivers</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014874" MajorTopicYN="N">Water Pollutants, Chemical</DescriptorName><QualifierName UI="Q000633" MajorTopicYN="Y">toxicity</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2006</Year><Month>3</Month><Day>10</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2006</Year><Month>6</Month><Day>19</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2006</Year><Month>6</Month><Day>20</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2006</Year><Month>8</Month><Day>5</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2007</Year><Month>12</Month><Day>6</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate 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Augagneur</name><name sortKey="Budzinski, H" sort="Budzinski, H" uniqKey="Budzinski H" first="H" last="Budzinski">H. Budzinski</name><name sortKey="Couteau, J" sort="Couteau, J" uniqKey="Couteau J" first="J" last="Couteau">J. Couteau</name><name sortKey="Denier, X" sort="Denier, X" uniqKey="Denier X" first="X" last="Denier">X. Denier</name><name sortKey="Devier, M H" sort="Devier, M H" uniqKey="Devier M" first="M H" last="Devier">M H Devier</name><name sortKey="Geffard, O" sort="Geffard, O" uniqKey="Geffard O" first="O" last="Geffard">O. Geffard</name><name sortKey="Lacroix, S" sort="Lacroix, S" uniqKey="Lacroix S" first="S" last="Lacroix">S. Lacroix</name><name sortKey="Le Menach, K" sort="Le Menach, K" uniqKey="Le Menach K" first="K" last="Le Menach">K. Le Menach</name><name sortKey="Peluhet, L" sort="Peluhet, L" uniqKey="Peluhet L" first="L" last="Peluhet">L. Peluhet</name><name sortKey="Pottier, D" sort="Pottier, D" uniqKey="Pottier D" first="D" last="Pottier">D. Pottier</name></noCountry><country name="France"><region name="Région Normandie"><name sortKey="Cachot, J" sort="Cachot, J" uniqKey="Cachot J" first="J" last="Cachot">J. Cachot</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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