Characterization of toxic effects of sediment-associated organic pollutants using the lambda transgenic medaka.
Identifieur interne : 000269 ( PubMed/Checkpoint ); précédent : 000268; suivant : 000270Characterization of toxic effects of sediment-associated organic pollutants using the lambda transgenic medaka.
Auteurs : Jerome Cachot [France] ; Mac Law ; Didier Pottier ; Laurent Peluhet ; Michelle Norris ; Helene Budzinski ; Richard WinnSource :
- Environmental science & technology [ 0013-936X ] ; 2007.
English descriptors
- KwdEn :
- Animals, Animals, Genetically Modified, Benzo(a)pyrene (chemistry), DNA Mutational Analysis, Environmental Monitoring (methods), Fishes, Gene Expression Regulation, Developmental, Geologic Sediments (analysis), Homozygote, Liver (metabolism), Mutation, Organic Chemicals (chemistry), Oryzias (genetics), Polychlorinated Biphenyls (chemistry), Time Factors, Water Pollutants (analysis), Water Pollutants (chemistry).
- MESH :
- chemical , analysis : Water Pollutants.
- chemical , chemistry : Benzo(a)pyrene, Organic Chemicals, Polychlorinated Biphenyls, Water Pollutants.
- analysis : Geologic Sediments.
- genetics : Oryzias.
- metabolism : Liver.
- methods : Environmental Monitoring.
- Animals, Animals, Genetically Modified, DNA Mutational Analysis, Fishes, Gene Expression Regulation, Developmental, Homozygote, Mutation, Time Factors.
Abstract
A novel sediment-contact assay using embryos of the transgenic medaka was developed to fully characterize the toxic effects induced by exposure to a mixture of organic pollutants in sediments. Embryos of the lambda transgenic medaka were exposed for 10 days to a clean reference sediment spiked with either the solvent alone, benzo[a]pyrene (B[a]P), or three concentrations (0.3x, 1x, and 2x) of an organic extract (OE) of sediments from the Seine estuary. The 1 x OE-spiked sediment contained concentrations of polycyclic aromatic hydrocarbons (PAHs) and polychlorinated biphenyls similar to those in field-collected sediment. Exposure to this sediment, but not to the B[a]P-spiked sediment, significantly increased embryo-larval mortality and prevalence of spinal deformities. Mutant frequency at the cII mutation target gene in the liver of 10-week-old medaka was significantly increased following exposure to either B[a]P or the three doses of OE. The predominant OE-induced liver mutations were G:C to T:A transversions, consistent with PAHs being the major contributors to the mutation induction. Liver and gonadal tumors were observed in 35-week-old medaka exposed to either B[a]P (1/25) or to the 1 x OE (1/24). The benefits of medaka as a fish model for toxicological assessment and the benefits of the cII mutation assay for mutation detection combine to provide comprehensive assessment of a wide range of genotoxic and nongenotoxic effects of aquatic pollutants.
PubMed: 18075095
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Characterization of toxic effects of sediment-associated organic pollutants using the lambda transgenic medaka.</title><author><name sortKey="Cachot, Jerome" sort="Cachot, Jerome" uniqKey="Cachot J" first="Jerome" last="Cachot">Jerome Cachot</name><affiliation wicri:level="3"><nlm:affiliation>University of Le Havre, LEMA EA-3222, 25 rue Philippe Lebon, B.P. 540, 76058 Le Havre, France. j.cachot@ism.u-bordeaux1.fr</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>University of Le Havre, LEMA EA-3222, 25 rue Philippe Lebon, B.P. 540, 76058 Le Havre</wicri:regionArea><placeName><region type="region" nuts="2">Région Normandie</region><region type="old region" nuts="2">Haute-Normandie</region><settlement type="city">Le Havre</settlement></placeName></affiliation></author><author><name sortKey="Law, Mac" sort="Law, Mac" uniqKey="Law M" first="Mac" last="Law">Mac Law</name></author><author><name sortKey="Pottier, Didier" sort="Pottier, Didier" uniqKey="Pottier D" first="Didier" last="Pottier">Didier Pottier</name></author><author><name sortKey="Peluhet, Laurent" sort="Peluhet, Laurent" uniqKey="Peluhet L" first="Laurent" last="Peluhet">Laurent Peluhet</name></author><author><name sortKey="Norris, Michelle" sort="Norris, Michelle" uniqKey="Norris M" first="Michelle" last="Norris">Michelle Norris</name></author><author><name sortKey="Budzinski, Helene" sort="Budzinski, Helene" uniqKey="Budzinski H" first="Helene" last="Budzinski">Helene Budzinski</name></author><author><name sortKey="Winn, Richard" sort="Winn, Richard" uniqKey="Winn R" first="Richard" last="Winn">Richard Winn</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2007">2007</date><idno type="RBID">pubmed:18075095</idno><idno type="pmid">18075095</idno><idno type="wicri:Area/PubMed/Corpus">000269</idno><idno type="wicri:Area/PubMed/Curation">000269</idno><idno 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last="Law">Mac Law</name></author><author><name sortKey="Pottier, Didier" sort="Pottier, Didier" uniqKey="Pottier D" first="Didier" last="Pottier">Didier Pottier</name></author><author><name sortKey="Peluhet, Laurent" sort="Peluhet, Laurent" uniqKey="Peluhet L" first="Laurent" last="Peluhet">Laurent Peluhet</name></author><author><name sortKey="Norris, Michelle" sort="Norris, Michelle" uniqKey="Norris M" first="Michelle" last="Norris">Michelle Norris</name></author><author><name sortKey="Budzinski, Helene" sort="Budzinski, Helene" uniqKey="Budzinski H" first="Helene" last="Budzinski">Helene Budzinski</name></author><author><name sortKey="Winn, Richard" sort="Winn, Richard" uniqKey="Winn R" first="Richard" last="Winn">Richard Winn</name></author></analytic><series><title level="j">Environmental science & technology</title><idno type="ISSN">0013-936X</idno><imprint><date when="2007" type="published">2007</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Animals, Genetically Modified</term><term>Benzo(a)pyrene (chemistry)</term><term>DNA Mutational Analysis</term><term>Environmental Monitoring (methods)</term><term>Fishes</term><term>Gene Expression Regulation, Developmental</term><term>Geologic Sediments (analysis)</term><term>Homozygote</term><term>Liver (metabolism)</term><term>Mutation</term><term>Organic Chemicals (chemistry)</term><term>Oryzias (genetics)</term><term>Polychlorinated Biphenyls (chemistry)</term><term>Time Factors</term><term>Water Pollutants (analysis)</term><term>Water Pollutants (chemistry)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Water Pollutants</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Benzo(a)pyrene</term><term>Organic Chemicals</term><term>Polychlorinated Biphenyls</term><term>Water Pollutants</term></keywords><keywords scheme="MESH" qualifier="analysis" xml:lang="en"><term>Geologic Sediments</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Oryzias</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Liver</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Environmental Monitoring</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Animals, Genetically Modified</term><term>DNA Mutational Analysis</term><term>Fishes</term><term>Gene Expression Regulation, Developmental</term><term>Homozygote</term><term>Mutation</term><term>Time Factors</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">A novel sediment-contact assay using embryos of the transgenic medaka was developed to fully characterize the toxic effects induced by exposure to a mixture of organic pollutants in sediments. Embryos of the lambda transgenic medaka were exposed for 10 days to a clean reference sediment spiked with either the solvent alone, benzo[a]pyrene (B[a]P), or three concentrations (0.3x, 1x, and 2x) of an organic extract (OE) of sediments from the Seine estuary. The 1 x OE-spiked sediment contained concentrations of polycyclic aromatic hydrocarbons (PAHs) and polychlorinated biphenyls similar to those in field-collected sediment. Exposure to this sediment, but not to the B[a]P-spiked sediment, significantly increased embryo-larval mortality and prevalence of spinal deformities. Mutant frequency at the cII mutation target gene in the liver of 10-week-old medaka was significantly increased following exposure to either B[a]P or the three doses of OE. The predominant OE-induced liver mutations were G:C to T:A transversions, consistent with PAHs being the major contributors to the mutation induction. Liver and gonadal tumors were observed in 35-week-old medaka exposed to either B[a]P (1/25) or to the 1 x OE (1/24). The benefits of medaka as a fish model for toxicological assessment and the benefits of the cII mutation assay for mutation detection combine to provide comprehensive assessment of a wide range of genotoxic and nongenotoxic effects of aquatic pollutants.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">18075095</PMID><DateCreated><Year>2007</Year><Month>12</Month><Day>13</Day></DateCreated><DateCompleted><Year>2008</Year><Month>02</Month><Day>11</Day></DateCompleted><DateRevised><Year>2013</Year><Month>11</Month><Day>21</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0013-936X</ISSN><JournalIssue CitedMedium="Print"><Volume>41</Volume><Issue>22</Issue><PubDate><Year>2007</Year><Month>Nov</Month><Day>15</Day></PubDate></JournalIssue><Title>Environmental science & technology</Title><ISOAbbreviation>Environ. Sci. Technol.</ISOAbbreviation></Journal><ArticleTitle>Characterization of toxic effects of sediment-associated organic pollutants using the lambda transgenic medaka.</ArticleTitle><Pagination><MedlinePgn>7830-6</MedlinePgn></Pagination><Abstract><AbstractText>A novel sediment-contact assay using embryos of the transgenic medaka was developed to fully characterize the toxic effects induced by exposure to a mixture of organic pollutants in sediments. Embryos of the lambda transgenic medaka were exposed for 10 days to a clean reference sediment spiked with either the solvent alone, benzo[a]pyrene (B[a]P), or three concentrations (0.3x, 1x, and 2x) of an organic extract (OE) of sediments from the Seine estuary. The 1 x OE-spiked sediment contained concentrations of polycyclic aromatic hydrocarbons (PAHs) and polychlorinated biphenyls similar to those in field-collected sediment. Exposure to this sediment, but not to the B[a]P-spiked sediment, significantly increased embryo-larval mortality and prevalence of spinal deformities. Mutant frequency at the cII mutation target gene in the liver of 10-week-old medaka was significantly increased following exposure to either B[a]P or the three doses of OE. The predominant OE-induced liver mutations were G:C to T:A transversions, consistent with PAHs being the major contributors to the mutation induction. Liver and gonadal tumors were observed in 35-week-old medaka exposed to either B[a]P (1/25) or to the 1 x OE (1/24). The benefits of medaka as a fish model for toxicological assessment and the benefits of the cII mutation assay for mutation detection combine to provide comprehensive assessment of a wide range of genotoxic and nongenotoxic effects of aquatic pollutants.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Cachot</LastName><ForeName>Jerome</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>University of Le Havre, LEMA EA-3222, 25 rue Philippe Lebon, B.P. 540, 76058 Le Havre, France. j.cachot@ism.u-bordeaux1.fr</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Law</LastName><ForeName>Mac</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>Pottier</LastName><ForeName>Didier</ForeName><Initials>D</Initials></Author><Author ValidYN="Y"><LastName>Peluhet</LastName><ForeName>Laurent</ForeName><Initials>L</Initials></Author><Author ValidYN="Y"><LastName>Norris</LastName><ForeName>Michelle</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>Budzinski</LastName><ForeName>Helene</ForeName><Initials>H</Initials></Author><Author ValidYN="Y"><LastName>Winn</LastName><ForeName>Richard</ForeName><Initials>R</Initials></Author></AuthorList><Language>ENG</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Environ Sci Technol</MedlineTA><NlmUniqueID>0213155</NlmUniqueID><ISSNLinking>0013-936X</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D009930">Organic Chemicals</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D014873">Water Pollutants</NameOfSubstance></Chemical><Chemical><RegistryNumber>3417WMA06D</RegistryNumber><NameOfSubstance UI="D001564">Benzo(a)pyrene</NameOfSubstance></Chemical><Chemical><RegistryNumber>DFC2HB4I0K</RegistryNumber><NameOfSubstance UI="D011078">Polychlorinated Biphenyls</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D030801" MajorTopicYN="N">Animals, Genetically Modified</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D001564" MajorTopicYN="N">Benzo(a)pyrene</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004252" MajorTopicYN="N">DNA Mutational Analysis</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D004784" MajorTopicYN="N">Environmental Monitoring</DescriptorName><QualifierName UI="Q000379" 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MajorTopicYN="Y">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D009990" MajorTopicYN="N">Oryzias</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011078" MajorTopicYN="N">Polychlorinated Biphenyls</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013997" MajorTopicYN="N">Time Factors</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014873" MajorTopicYN="N">Water Pollutants</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="N">analysis</QualifierName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2007</Year><Month>12</Month><Day>14</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2008</Year><Month>2</Month><Day>12</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2007</Year><Month>12</Month><Day>14</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">18075095</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>France</li></country><region><li>Haute-Normandie</li><li>Région Normandie</li></region><settlement><li>Le Havre</li></settlement></list><tree><noCountry><name sortKey="Budzinski, Helene" sort="Budzinski, Helene" uniqKey="Budzinski H" first="Helene" last="Budzinski">Helene Budzinski</name><name sortKey="Law, Mac" sort="Law, Mac" uniqKey="Law M" first="Mac" last="Law">Mac Law</name><name sortKey="Norris, Michelle" sort="Norris, Michelle" uniqKey="Norris M" first="Michelle" last="Norris">Michelle Norris</name><name sortKey="Peluhet, Laurent" sort="Peluhet, Laurent" uniqKey="Peluhet L" first="Laurent" last="Peluhet">Laurent Peluhet</name><name sortKey="Pottier, Didier" sort="Pottier, Didier" uniqKey="Pottier D" first="Didier" last="Pottier">Didier Pottier</name><name sortKey="Winn, Richard" sort="Winn, Richard" uniqKey="Winn R" first="Richard" last="Winn">Richard Winn</name></noCountry><country name="France"><region name="Région Normandie"><name sortKey="Cachot, Jerome" sort="Cachot, Jerome" uniqKey="Cachot J" first="Jerome" last="Cachot">Jerome Cachot</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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