Different modalities of intercellular membrane exchanges mediate cell-to-cell p-glycoprotein transfers in MCF-7 breast cancer cells.
Identifieur interne : 000191 ( PubMed/Checkpoint ); précédent : 000190; suivant : 000192Different modalities of intercellular membrane exchanges mediate cell-to-cell p-glycoprotein transfers in MCF-7 breast cancer cells.
Auteurs : Jennifer Pasquier [France] ; Ludovic Galas ; Céline Boulangé-Lecomte ; Damien Rioult ; Florence Bultelle ; Pierre Magal ; Glenn Webb ; Frank Le FollSource :
- The Journal of biological chemistry [ 1083-351X ] ; 2012.
English descriptors
- KwdEn :
- Breast Neoplasms (genetics), Breast Neoplasms (metabolism), Breast Neoplasms (pathology), Cell Communication, Cell Line, Tumor, Cell-Derived Microparticles (genetics), Cell-Derived Microparticles (metabolism), Cell-Derived Microparticles (pathology), Drug Resistance, Neoplasm, Female, Humans, P-Glycoprotein (genetics), P-Glycoprotein (metabolism), Protein Transport (genetics).
- MESH :
- chemical , genetics : P-Glycoprotein.
- genetics : Breast Neoplasms, Cell-Derived Microparticles, Protein Transport.
- metabolism : Breast Neoplasms, Cell-Derived Microparticles, P-Glycoprotein.
- pathology : Breast Neoplasms, Cell-Derived Microparticles.
- Cell Communication, Cell Line, Tumor, Drug Resistance, Neoplasm, Female, Humans.
Abstract
Multi-drug resistance (MDR) is a phenomenon by which tumor cells exhibit resistance to a variety of chemically unrelated chemotherapeutic drugs. The classical form of multidrug resistance is connected to overexpression of membrane P-glycoprotein (P-gp), which acts as an energy dependent drug efflux pump. P-glycoprotein expression is known to be controlled by genetic and epigenetic mechanisms. Until now processes of P-gp gene up-regulation and resistant cell selection were considered sufficient to explain the emergence of MDR phenotype within a cell population. Recently, however, "non-genetic" acquisitions of MDR by cell-to-cell P-gp transfers have been pointed out. In the present study we show that intercellular transfers of functional P-gp occur by two different but complementary modalities through donor-recipient cells interactions in the absence of drug selection pressure. P-glycoprotein and drug efflux activity transfers were followed over 7 days by confocal microscopy and flow cytometry in drug-sensitive parental MCF-7 breast cancer cells co-cultured with P-gp overexpressing resistant variants. An early process of remote transfer was established based on the release and binding of P-gp-containing microparticles. Microparticle-mediated transfers were detected after only 4 h of incubation. We also identify an alternative mode of transfer by contact, consisting of cell-to-cell P-gp trafficking by tunneling nanotubes bridging neighboring cells. Our findings supply new mechanistic evidences for the extragenetic emergence of MDR in cancer cells and indicate that new treatment strategies designed to overcome MDR may include inhibition of both microparticles and Tunneling nanotube-mediated intercellular P-gp transfers.
DOI: 10.1074/jbc.M111.312157
PubMed: 22228759
Affiliations:
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sortKey="Boulange Lecomte, Celine" sort="Boulange Lecomte, Celine" uniqKey="Boulange Lecomte C" first="Céline" last="Boulangé-Lecomte">Céline Boulangé-Lecomte</name></author><author><name sortKey="Rioult, Damien" sort="Rioult, Damien" uniqKey="Rioult D" first="Damien" last="Rioult">Damien Rioult</name></author><author><name sortKey="Bultelle, Florence" sort="Bultelle, Florence" uniqKey="Bultelle F" first="Florence" last="Bultelle">Florence Bultelle</name></author><author><name sortKey="Magal, Pierre" sort="Magal, Pierre" uniqKey="Magal P" first="Pierre" last="Magal">Pierre Magal</name></author><author><name sortKey="Webb, Glenn" sort="Webb, Glenn" uniqKey="Webb G" first="Glenn" last="Webb">Glenn Webb</name></author><author><name sortKey="Le Foll, Frank" sort="Le Foll, Frank" uniqKey="Le Foll F" first="Frank" last="Le Foll">Frank Le Foll</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2012">2012</date><idno 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region" nuts="2">Haute-Normandie</region><settlement type="city">Le Havre</settlement></placeName></affiliation></author><author><name sortKey="Galas, Ludovic" sort="Galas, Ludovic" uniqKey="Galas L" first="Ludovic" last="Galas">Ludovic Galas</name></author><author><name sortKey="Boulange Lecomte, Celine" sort="Boulange Lecomte, Celine" uniqKey="Boulange Lecomte C" first="Céline" last="Boulangé-Lecomte">Céline Boulangé-Lecomte</name></author><author><name sortKey="Rioult, Damien" sort="Rioult, Damien" uniqKey="Rioult D" first="Damien" last="Rioult">Damien Rioult</name></author><author><name sortKey="Bultelle, Florence" sort="Bultelle, Florence" uniqKey="Bultelle F" first="Florence" last="Bultelle">Florence Bultelle</name></author><author><name sortKey="Magal, Pierre" sort="Magal, Pierre" uniqKey="Magal P" first="Pierre" last="Magal">Pierre Magal</name></author><author><name sortKey="Webb, Glenn" sort="Webb, Glenn" uniqKey="Webb G" first="Glenn" last="Webb">Glenn Webb</name></author><author><name sortKey="Le Foll, Frank" sort="Le Foll, Frank" uniqKey="Le Foll F" first="Frank" last="Le Foll">Frank Le Foll</name></author></analytic><series><title level="j">The Journal of biological chemistry</title><idno type="eISSN">1083-351X</idno><imprint><date when="2012" type="published">2012</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Breast Neoplasms (genetics)</term><term>Breast Neoplasms (metabolism)</term><term>Breast Neoplasms (pathology)</term><term>Cell Communication</term><term>Cell Line, Tumor</term><term>Cell-Derived Microparticles (genetics)</term><term>Cell-Derived Microparticles (metabolism)</term><term>Cell-Derived Microparticles (pathology)</term><term>Drug Resistance, Neoplasm</term><term>Female</term><term>Humans</term><term>P-Glycoprotein (genetics)</term><term>P-Glycoprotein (metabolism)</term><term>Protein Transport (genetics)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>P-Glycoprotein</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Breast Neoplasms</term><term>Cell-Derived Microparticles</term><term>Protein Transport</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Breast Neoplasms</term><term>Cell-Derived Microparticles</term><term>P-Glycoprotein</term></keywords><keywords scheme="MESH" qualifier="pathology" xml:lang="en"><term>Breast Neoplasms</term><term>Cell-Derived Microparticles</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Cell Communication</term><term>Cell Line, Tumor</term><term>Drug Resistance, Neoplasm</term><term>Female</term><term>Humans</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Multi-drug resistance (MDR) is a phenomenon by which tumor cells exhibit resistance to a variety of chemically unrelated chemotherapeutic drugs. The classical form of multidrug resistance is connected to overexpression of membrane P-glycoprotein (P-gp), which acts as an energy dependent drug efflux pump. P-glycoprotein expression is known to be controlled by genetic and epigenetic mechanisms. Until now processes of P-gp gene up-regulation and resistant cell selection were considered sufficient to explain the emergence of MDR phenotype within a cell population. Recently, however, "non-genetic" acquisitions of MDR by cell-to-cell P-gp transfers have been pointed out. In the present study we show that intercellular transfers of functional P-gp occur by two different but complementary modalities through donor-recipient cells interactions in the absence of drug selection pressure. P-glycoprotein and drug efflux activity transfers were followed over 7 days by confocal microscopy and flow cytometry in drug-sensitive parental MCF-7 breast cancer cells co-cultured with P-gp overexpressing resistant variants. An early process of remote transfer was established based on the release and binding of P-gp-containing microparticles. Microparticle-mediated transfers were detected after only 4 h of incubation. We also identify an alternative mode of transfer by contact, consisting of cell-to-cell P-gp trafficking by tunneling nanotubes bridging neighboring cells. Our findings supply new mechanistic evidences for the extragenetic emergence of MDR in cancer cells and indicate that new treatment strategies designed to overcome MDR may include inhibition of both microparticles and Tunneling nanotube-mediated intercellular P-gp transfers.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">22228759</PMID><DateCreated><Year>2012</Year><Month>3</Month><Day>5</Day></DateCreated><DateCompleted><Year>2012</Year><Month>04</Month><Day>24</Day></DateCompleted><DateRevised><Year>2015</Year><Month>1</Month><Day>28</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1083-351X</ISSN><JournalIssue CitedMedium="Internet"><Volume>287</Volume><Issue>10</Issue><PubDate><Year>2012</Year><Month>Mar</Month><Day>2</Day></PubDate></JournalIssue><Title>The Journal of biological chemistry</Title><ISOAbbreviation>J. Biol. Chem.</ISOAbbreviation></Journal><ArticleTitle>Different modalities of intercellular membrane exchanges mediate cell-to-cell p-glycoprotein transfers in MCF-7 breast cancer cells.</ArticleTitle><Pagination><MedlinePgn>7374-87</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1074/jbc.M111.312157</ELocationID><Abstract><AbstractText>Multi-drug resistance (MDR) is a phenomenon by which tumor cells exhibit resistance to a variety of chemically unrelated chemotherapeutic drugs. The classical form of multidrug resistance is connected to overexpression of membrane P-glycoprotein (P-gp), which acts as an energy dependent drug efflux pump. P-glycoprotein expression is known to be controlled by genetic and epigenetic mechanisms. Until now processes of P-gp gene up-regulation and resistant cell selection were considered sufficient to explain the emergence of MDR phenotype within a cell population. Recently, however, "non-genetic" acquisitions of MDR by cell-to-cell P-gp transfers have been pointed out. In the present study we show that intercellular transfers of functional P-gp occur by two different but complementary modalities through donor-recipient cells interactions in the absence of drug selection pressure. P-glycoprotein and drug efflux activity transfers were followed over 7 days by confocal microscopy and flow cytometry in drug-sensitive parental MCF-7 breast cancer cells co-cultured with P-gp overexpressing resistant variants. An early process of remote transfer was established based on the release and binding of P-gp-containing microparticles. Microparticle-mediated transfers were detected after only 4 h of incubation. We also identify an alternative mode of transfer by contact, consisting of cell-to-cell P-gp trafficking by tunneling nanotubes bridging neighboring cells. Our findings supply new mechanistic evidences for the extragenetic emergence of MDR in cancer cells and indicate that new treatment strategies designed to overcome MDR may include inhibition of both microparticles and Tunneling nanotube-mediated intercellular P-gp transfers.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Pasquier</LastName><ForeName>Jennifer</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Laboratory of Ecotoxicology, University of Le Havre, 76058 Le Havre, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Galas</LastName><ForeName>Ludovic</ForeName><Initials>L</Initials></Author><Author ValidYN="Y"><LastName>Boulangé-Lecomte</LastName><ForeName>Céline</ForeName><Initials>C</Initials></Author><Author ValidYN="Y"><LastName>Rioult</LastName><ForeName>Damien</ForeName><Initials>D</Initials></Author><Author ValidYN="Y"><LastName>Bultelle</LastName><ForeName>Florence</ForeName><Initials>F</Initials></Author><Author ValidYN="Y"><LastName>Magal</LastName><ForeName>Pierre</ForeName><Initials>P</Initials></Author><Author ValidYN="Y"><LastName>Webb</LastName><ForeName>Glenn</ForeName><Initials>G</Initials></Author><Author ValidYN="Y"><LastName>Le Foll</LastName><ForeName>Frank</ForeName><Initials>F</Initials></Author></AuthorList><Language>ENG</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2012</Year><Month>Jan</Month><Day>06</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>J Biol Chem</MedlineTA><NlmUniqueID>2985121R</NlmUniqueID><ISSNLinking>0021-9258</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D020168">P-Glycoprotein</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><CommentsCorrectionsList><CommentsCorrections RefType="Cites"><RefSource>J Biol Chem. 2001 May 4;276(18):14581-7</RefSource><PMID Version="1">11297522</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Eur J Pharm Sci. 2000 Nov;12(1):31-40</RefSource><PMID Version="1">11121731</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Can J Physiol Pharmacol. 2002 Nov;80(11):1054-63</RefSource><PMID Version="1">12489924</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Cancer Res. 2003 Aug 1;63(15):4331-7</RefSource><PMID Version="1">12907600</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Oncogene. 2003 Oct 20;22(47):7468-85</RefSource><PMID Version="1">14576852</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Science. 2004 Feb 13;303(5660):1007-10</RefSource><PMID Version="1">14963329</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Oncol Rep. 2004 May;11(5):1091-5</RefSource><PMID Version="1">15069552</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Biochim Biophys Acta. 1976 Nov 11;455(1):152-62</RefSource><PMID Version="1">990323</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Nature. 1985 Aug 29-Sep 4;316(6031):817-9</RefSource><PMID Version="1">2863759</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>N Engl J Med. 1987 May 28;316(22):1388-93</RefSource><PMID Version="1">3553950</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>J Natl Cancer Inst. 1989 Jan 18;81(2):116-24</RefSource><PMID Version="1">2562856</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Cancer Res. 1990 Apr 1;50(7):2191-7</RefSource><PMID Version="1">1969329</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Blood. 1994 May 1;83(9):2451-8</RefSource><PMID Version="1">7513198</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Hematol Oncol Clin North Am. 1995 Apr;9(2):251-73</RefSource><PMID Version="1">7642464</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Annu Rev Genet. 1995;29:607-49</RefSource><PMID Version="1">8825488</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Mol Cells. 1999 Jun 30;9(3):314-9</RefSource><PMID Version="1">10420992</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>J Biol Chem. 2005 Jan 28;280(4):2857-62</RefSource><PMID Version="1">15485807</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Proc Natl Acad Sci U S A. 2005 Feb 8;102(6):1933-8</RefSource><PMID Version="1">15671173</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Toxicol Appl Pharmacol. 2005 May 1;204(3):216-37</RefSource><PMID Version="1">15845415</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>J Chemother. 2005 Apr;17(2):215-23</RefSource><PMID Version="1">15920909</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Biochem Biophys Res Commun. 2005 Sep 9;334(4):1266-78</RefSource><PMID Version="1">16039989</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Mol Cancer Ther. 2005 Oct;4(10):1595-604</RefSource><PMID Version="1">16227410</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Oncogene. 2005 Dec 1;24(54):8061-75</RefSource><PMID Version="1">16091741</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Cancer Lett. 2006 Jan 8;231(1):87-93</RefSource><PMID Version="1">16356834</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>FEBS Lett. 2006 Sep 4;580(20):4953-8</RefSource><PMID Version="1">16930597</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Drug Resist Updat. 2007 Feb-Apr;10(1-2):59-67</RefSource><PMID Version="1">17350322</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Curr Protoc Cell Biol. 2006 Apr;Chapter 3:Unit 3.22</RefSource><PMID Version="1">18228490</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>J Clin Pharmacol. 2008 Mar;48(3):365-78</RefSource><PMID Version="1">18156365</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Curr Drug Metab. 2008 Feb;9(2):167-74</RefSource><PMID Version="1">18288958</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Histochem Cell Biol. 2008 May;129(5):539-50</RefSource><PMID Version="1">18386044</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Curr Opin Cell Biol. 2008 Aug;20(4):470-5</RefSource><PMID Version="1">18456488</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Mol Cancer Ther. 2008 Jul;7(7):2152-9</RefSource><PMID Version="1">18645025</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Xenobiotica. 2008 Jul;38(7-8):802-32</RefSource><PMID Version="1">18668431</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Proteomics. 2008 Oct;8(19):4083-99</RefSource><PMID Version="1">18780348</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Epigenetics. 2008 Sep;3(5):270-80</RefSource><PMID Version="1">19001875</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>PLoS One. 2008;3(12):e3894</RefSource><PMID Version="1">19079610</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Proteomics. 2009 May;9(10):2820-35</RefSource><PMID Version="1">19415654</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Nat Rev Immunol. 2009 Aug;9(8):581-93</RefSource><PMID Version="1">19498381</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Leukemia. 2009 Sep;23(9):1643-9</RefSource><PMID Version="1">19369960</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>J Neurosci. 2010 Jan 27;30(4):1417-25</RefSource><PMID Version="1">20107068</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>J Cereb Blood Flow Metab. 2010 Sep;30(9):1593-7</RefSource><PMID Version="1">20628400</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Biol Direct. 2011;6:5</RefSource><PMID Version="1">21269489</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Am J Physiol. 1999 Dec;277(6 Pt 2):F832-40</RefSource><PMID Version="1">10600929</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Nat Med. 2001 May;7(5):584-90</RefSource><PMID Version="1">11329060</PMID></CommentsCorrections></CommentsCorrectionsList><MeshHeadingList><MeshHeading><DescriptorName UI="D001943" MajorTopicYN="N">Breast Neoplasms</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName><QualifierName UI="Q000473" MajorTopicYN="N">pathology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002450" MajorTopicYN="Y">Cell Communication</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D045744" MajorTopicYN="N">Cell Line, Tumor</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D055252" MajorTopicYN="N">Cell-Derived Microparticles</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName><QualifierName UI="Q000473" MajorTopicYN="N">pathology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D019008" MajorTopicYN="Y">Drug Resistance, Neoplasm</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005260" MajorTopicYN="N">Female</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D020168" MajorTopicYN="N">P-Glycoprotein</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D021381" MajorTopicYN="N">Protein Transport</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading></MeshHeadingList><OtherID Source="NLM">PMC3293537</OtherID></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2012</Year><Month>1</Month><Day>10</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate 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first="Florence" last="Bultelle">Florence Bultelle</name><name sortKey="Galas, Ludovic" sort="Galas, Ludovic" uniqKey="Galas L" first="Ludovic" last="Galas">Ludovic Galas</name><name sortKey="Le Foll, Frank" sort="Le Foll, Frank" uniqKey="Le Foll F" first="Frank" last="Le Foll">Frank Le Foll</name><name sortKey="Magal, Pierre" sort="Magal, Pierre" uniqKey="Magal P" first="Pierre" last="Magal">Pierre Magal</name><name sortKey="Rioult, Damien" sort="Rioult, Damien" uniqKey="Rioult D" first="Damien" last="Rioult">Damien Rioult</name><name sortKey="Webb, Glenn" sort="Webb, Glenn" uniqKey="Webb G" first="Glenn" last="Webb">Glenn Webb</name></noCountry><country name="France"><region name="Région Normandie"><name sortKey="Pasquier, Jennifer" sort="Pasquier, Jennifer" uniqKey="Pasquier J" first="Jennifer" last="Pasquier">Jennifer Pasquier</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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