TP53 and FGFR3 Gene Mutation Assessment in Urine: Pilot Study for Bladder Cancer Diagnosis.
Identifieur interne : 000074 ( PubMed/Checkpoint ); précédent : 000073; suivant : 000075TP53 and FGFR3 Gene Mutation Assessment in Urine: Pilot Study for Bladder Cancer Diagnosis.
Auteurs : Nicolas Noel [France] ; Jerome Couteau [France] ; Geraldine Maillet [France] ; Françoise Gobet [France] ; Françoise D'Aloisio [France] ; Christophe Minier [France] ; Christian Pfister [France]Source :
- Anticancer research [ 1791-7530 ] ; 2015.
English descriptors
- KwdEn :
- DNA Mutational Analysis, Electrophoresis, Agar Gel, Humans, Mutation (genetics), Neoplasm Staging, Phenotype, Pilot Projects, Receptor, Fibroblast Growth Factor, Type 3 (genetics), Receptor, Fibroblast Growth Factor, Type 3 (urine), Tumor Suppressor Protein p53 (genetics), Tumor Suppressor Protein p53 (urine), Urinary Bladder Neoplasms (genetics), Urinary Bladder Neoplasms (pathology), Urinary Bladder Neoplasms (urine).
- MESH :
- chemical , genetics : Receptor, Fibroblast Growth Factor, Type 3, Tumor Suppressor Protein p53.
- genetics : Mutation, Urinary Bladder Neoplasms.
- pathology : Urinary Bladder Neoplasms.
- chemical , urine : Receptor, Fibroblast Growth Factor, Type 3, Tumor Suppressor Protein p53, Urinary Bladder Neoplasms.
- DNA Mutational Analysis, Electrophoresis, Agar Gel, Humans, Neoplasm Staging, Phenotype, Pilot Projects.
Abstract
To assess, in a prospective clinical research study, a new non-invasive and reliable test to accurately detect tumor protein 53 (TP53) and fibroblast growth factor receptor-3 (FGFR3) mutations in cells in urine.
PubMed: 26254388
Affiliations:
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type="city">Le Havre</settlement></placeName></affiliation></author><author><name sortKey="Maillet, Geraldine" sort="Maillet, Geraldine" uniqKey="Maillet G" first="Geraldine" last="Maillet">Geraldine Maillet</name><affiliation wicri:level="1"><nlm:affiliation>TOXEM Laboratory, Le Havre, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>TOXEM Laboratory, Le Havre</wicri:regionArea><placeName><settlement type="city">Le Havre</settlement></placeName></affiliation></author><author><name sortKey="Gobet, Francoise" sort="Gobet, Francoise" uniqKey="Gobet F" first="Françoise" last="Gobet">Françoise Gobet</name><affiliation wicri:level="1"><nlm:affiliation>Department of Pathology, Rouen University Hospital, Rouen, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Department of Pathology, Rouen University Hospital, Rouen</wicri:regionArea><placeName><settlement type="city">Rouen</settlement></placeName></affiliation></author><author><name sortKey="D Aloisio, Francoise" sort="D Aloisio, Francoise" uniqKey="D Aloisio F" first="Françoise" last="D'Aloisio">Françoise D'Aloisio</name><affiliation wicri:level="1"><nlm:affiliation>Clinical Investigation Center, Inserm 6204, Urological Cancerology, Rouen, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Clinical Investigation Center, Inserm 6204, Urological Cancerology, Rouen</wicri:regionArea><placeName><settlement type="city">Rouen</settlement></placeName></affiliation></author><author><name sortKey="Minier, Christophe" sort="Minier, Christophe" uniqKey="Minier C" first="Christophe" last="Minier">Christophe Minier</name><affiliation wicri:level="1"><nlm:affiliation>UMR-I 02 SEBIO, INERIS, BP540, Le Havre, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>UMR-I 02 SEBIO, INERIS, BP540, Le Havre</wicri:regionArea><placeName><settlement type="city">Le Havre</settlement></placeName></affiliation></author><author><name sortKey="Pfister, Christian" sort="Pfister, Christian" uniqKey="Pfister C" first="Christian" last="Pfister">Christian Pfister</name><affiliation wicri:level="1"><nlm:affiliation>Department of Urology, Rouen University Hospital, Rouen, France Clinical Investigation Center, Inserm 6204, Urological Cancerology, Rouen, France Christian.Pfister@chu-rouen.fr.</nlm:affiliation><country wicri:rule="url">France</country><wicri:regionArea>Department of Urology, Rouen University Hospital, Rouen, France Clinical Investigation Center, Inserm 6204, Urological Cancerology, Rouen</wicri:regionArea><placeName><settlement type="city">Rouen</settlement></placeName></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2015">2015</date><idno type="RBID">pubmed:26254388</idno><idno type="pmid">26254388</idno><idno type="wicri:Area/PubMed/Corpus">000068</idno><idno type="wicri:Area/PubMed/Curation">000068</idno><idno type="wicri:Area/PubMed/Checkpoint">000068</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">TP53 and FGFR3 Gene Mutation Assessment in Urine: Pilot Study for Bladder Cancer Diagnosis.</title><author><name sortKey="Noel, Nicolas" sort="Noel, Nicolas" uniqKey="Noel N" first="Nicolas" last="Noel">Nicolas Noel</name><affiliation wicri:level="1"><nlm:affiliation>Department of Urology, Rouen University Hospital, Rouen, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Department of Urology, Rouen University Hospital, Rouen</wicri:regionArea><placeName><settlement type="city">Rouen</settlement></placeName></affiliation></author><author><name sortKey="Couteau, Jerome" sort="Couteau, Jerome" uniqKey="Couteau J" first="Jerome" last="Couteau">Jerome Couteau</name><affiliation wicri:level="1"><nlm:affiliation>TOXEM Laboratory, Le Havre, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>TOXEM Laboratory, Le Havre</wicri:regionArea><placeName><settlement type="city">Le Havre</settlement></placeName></affiliation></author><author><name sortKey="Maillet, Geraldine" sort="Maillet, Geraldine" uniqKey="Maillet G" first="Geraldine" last="Maillet">Geraldine Maillet</name><affiliation wicri:level="1"><nlm:affiliation>TOXEM Laboratory, Le Havre, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>TOXEM Laboratory, Le Havre</wicri:regionArea><placeName><settlement type="city">Le Havre</settlement></placeName></affiliation></author><author><name sortKey="Gobet, Francoise" sort="Gobet, Francoise" uniqKey="Gobet F" first="Françoise" last="Gobet">Françoise Gobet</name><affiliation wicri:level="1"><nlm:affiliation>Department of Pathology, Rouen University Hospital, Rouen, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Department of Pathology, Rouen University Hospital, Rouen</wicri:regionArea><placeName><settlement type="city">Rouen</settlement></placeName></affiliation></author><author><name sortKey="D Aloisio, Francoise" sort="D Aloisio, Francoise" uniqKey="D Aloisio F" first="Françoise" last="D'Aloisio">Françoise D'Aloisio</name><affiliation wicri:level="1"><nlm:affiliation>Clinical Investigation Center, Inserm 6204, Urological Cancerology, Rouen, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Clinical Investigation Center, Inserm 6204, Urological Cancerology, Rouen</wicri:regionArea><placeName><settlement type="city">Rouen</settlement></placeName></affiliation></author><author><name sortKey="Minier, Christophe" sort="Minier, Christophe" uniqKey="Minier C" first="Christophe" last="Minier">Christophe Minier</name><affiliation wicri:level="1"><nlm:affiliation>UMR-I 02 SEBIO, INERIS, BP540, Le Havre, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>UMR-I 02 SEBIO, INERIS, BP540, Le Havre</wicri:regionArea><placeName><settlement type="city">Le Havre</settlement></placeName></affiliation></author><author><name sortKey="Pfister, Christian" sort="Pfister, Christian" uniqKey="Pfister C" first="Christian" last="Pfister">Christian Pfister</name><affiliation wicri:level="1"><nlm:affiliation>Department of Urology, Rouen University Hospital, Rouen, France Clinical Investigation Center, Inserm 6204, Urological Cancerology, Rouen, France Christian.Pfister@chu-rouen.fr.</nlm:affiliation><country wicri:rule="url">France</country><wicri:regionArea>Department of Urology, Rouen University Hospital, Rouen, France Clinical Investigation Center, Inserm 6204, Urological Cancerology, Rouen</wicri:regionArea><placeName><settlement type="city">Rouen</settlement></placeName></affiliation></author></analytic><series><title level="j">Anticancer research</title><idno type="eISSN">1791-7530</idno><imprint><date when="2015" type="published">2015</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>DNA Mutational Analysis</term><term>Electrophoresis, Agar Gel</term><term>Humans</term><term>Mutation (genetics)</term><term>Neoplasm Staging</term><term>Phenotype</term><term>Pilot Projects</term><term>Receptor, Fibroblast Growth Factor, Type 3 (genetics)</term><term>Receptor, Fibroblast Growth Factor, Type 3 (urine)</term><term>Tumor Suppressor Protein p53 (genetics)</term><term>Tumor Suppressor Protein p53 (urine)</term><term>Urinary Bladder Neoplasms (genetics)</term><term>Urinary Bladder Neoplasms (pathology)</term><term>Urinary Bladder Neoplasms (urine)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Receptor, Fibroblast Growth Factor, Type 3</term><term>Tumor Suppressor Protein p53</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Mutation</term><term>Urinary Bladder Neoplasms</term></keywords><keywords scheme="MESH" qualifier="pathology" xml:lang="en"><term>Urinary Bladder Neoplasms</term></keywords><keywords scheme="MESH" type="chemical" qualifier="urine" xml:lang="en"><term>Receptor, Fibroblast Growth Factor, Type 3</term><term>Tumor Suppressor Protein p53</term><term>Urinary Bladder Neoplasms</term></keywords><keywords scheme="MESH" xml:lang="en"><term>DNA Mutational Analysis</term><term>Electrophoresis, Agar Gel</term><term>Humans</term><term>Neoplasm Staging</term><term>Phenotype</term><term>Pilot Projects</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">To assess, in a prospective clinical research study, a new non-invasive and reliable test to accurately detect tumor protein 53 (TP53) and fibroblast growth factor receptor-3 (FGFR3) mutations in cells in urine.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">26254388</PMID><DateCreated><Year>2015</Year><Month>8</Month><Day>8</Day></DateCreated><DateCompleted><Year>2016</Year><Month>04</Month><Day>28</Day></DateCompleted><DateRevised><Year>2015</Year><Month>8</Month><Day>8</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Electronic">1791-7530</ISSN><JournalIssue CitedMedium="Internet"><Volume>35</Volume><Issue>9</Issue><PubDate><Year>2015</Year><Month>Sep</Month></PubDate></JournalIssue><Title>Anticancer research</Title><ISOAbbreviation>Anticancer Res.</ISOAbbreviation></Journal><ArticleTitle>TP53 and FGFR3 Gene Mutation Assessment in Urine: Pilot Study for Bladder Cancer Diagnosis.</ArticleTitle><Pagination><MedlinePgn>4915-21</MedlinePgn></Pagination><Abstract><AbstractText Label="AIM" NlmCategory="OBJECTIVE">To assess, in a prospective clinical research study, a new non-invasive and reliable test to accurately detect tumor protein 53 (TP53) and fibroblast growth factor receptor-3 (FGFR3) mutations in cells in urine.</AbstractText><AbstractText Label="MATERIALS AND METHODS" NlmCategory="METHODS">TP53 mutations were analyzed using the functional analysis of separated allele in yeast (FASAY) method, which allows functional analysis of the P53 protein, and FGFR3 mutations were assessed with the SNaPshot system, detecting the eight most frequent point-mutations of this gene. Chi-square test or Fisher's exact test were used to compare TP53 and FGFR3 mutations in the tumors according to tumor stage and grade.</AbstractText><AbstractText Label="RESULTS" NlmCategory="RESULTS">TP53 and FGFR3 mutations in bladder tumors increased and decreased respectively with increasing tumor stage and cellular grade (p<0.05 and p<0.001, respectively). A total of 103 tumor/urinary sediment couples were analyzed. TP53 or FGFR3 mutations were observed in 76 tumors. The sensitivity for the detection of this type of mutation in urine was 46%, the specificity was 81%, the positive predictive value was 94% and the negative predictive value was 37%.</AbstractText><AbstractText Label="CONCLUSION" NlmCategory="CONCLUSIONS">Our original data confirmed the feasibility of TP53 and FGFR3 mutation detection in urine sediment. These measurements, together with urine cytology, may increase tumor detection. The sensitivity of the TP53/FGFR3 phenotype test in the urine was less than 50% and was not able to replace standard cystoscopy in the diagnosis of bladder tumors.</AbstractText><CopyrightInformation>Copyright© 2015 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Noel</LastName><ForeName>Nicolas</ForeName><Initials>N</Initials><AffiliationInfo><Affiliation>Department of Urology, Rouen University Hospital, Rouen, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Couteau</LastName><ForeName>Jerome</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>TOXEM Laboratory, Le Havre, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Maillet</LastName><ForeName>Geraldine</ForeName><Initials>G</Initials><AffiliationInfo><Affiliation>TOXEM Laboratory, Le Havre, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Gobet</LastName><ForeName>Françoise</ForeName><Initials>F</Initials><AffiliationInfo><Affiliation>Department of Pathology, Rouen University Hospital, Rouen, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>D'Aloisio</LastName><ForeName>Françoise</ForeName><Initials>F</Initials><AffiliationInfo><Affiliation>Clinical Investigation Center, Inserm 6204, Urological Cancerology, Rouen, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Minier</LastName><ForeName>Christophe</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>UMR-I 02 SEBIO, INERIS, BP540, Le Havre, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Pfister</LastName><ForeName>Christian</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>Department of Urology, Rouen University Hospital, Rouen, France Clinical Investigation Center, Inserm 6204, Urological Cancerology, Rouen, France Christian.Pfister@chu-rouen.fr.</Affiliation></AffiliationInfo></Author></AuthorList><Language>ENG</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>Greece</Country><MedlineTA>Anticancer Res</MedlineTA><NlmUniqueID>8102988</NlmUniqueID><ISSNLinking>0250-7005</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D016159">Tumor Suppressor Protein p53</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 2.7.10.1</RegistryNumber><NameOfSubstance UI="D051498">Receptor, Fibroblast Growth Factor, Type 3</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D004252" MajorTopicYN="N">DNA Mutational Analysis</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D004587" MajorTopicYN="N">Electrophoresis, Agar Gel</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D009154" MajorTopicYN="N">Mutation</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D009367" MajorTopicYN="N">Neoplasm Staging</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010641" MajorTopicYN="N">Phenotype</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010865" MajorTopicYN="N">Pilot Projects</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D051498" MajorTopicYN="N">Receptor, Fibroblast Growth Factor, Type 3</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000652" MajorTopicYN="Y">urine</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D016159" MajorTopicYN="N">Tumor Suppressor Protein p53</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000652" MajorTopicYN="Y">urine</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D001749" MajorTopicYN="N">Urinary Bladder Neoplasms</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000473" MajorTopicYN="N">pathology</QualifierName><QualifierName UI="Q000652" MajorTopicYN="Y">urine</QualifierName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">FGFR3</Keyword><Keyword MajorTopicYN="N">TP53</Keyword><Keyword MajorTopicYN="N">bladder cancer</Keyword><Keyword MajorTopicYN="N">mutation phenotype</Keyword><Keyword MajorTopicYN="N">urine sediment</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2015</Year><Month>8</Month><Day>9</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2015</Year><Month>8</Month><Day>9</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2016</Year><Month>4</Month><Day>29</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">26254388</ArticleId><ArticleId IdType="pii">35/9/4915</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>France</li></country><settlement><li>Le Havre</li><li>Rouen</li></settlement></list><tree><country name="France"><noRegion><name sortKey="Noel, Nicolas" sort="Noel, Nicolas" uniqKey="Noel N" first="Nicolas" last="Noel">Nicolas Noel</name></noRegion><name sortKey="Couteau, Jerome" sort="Couteau, Jerome" uniqKey="Couteau J" first="Jerome" last="Couteau">Jerome Couteau</name><name sortKey="D Aloisio, Francoise" sort="D Aloisio, Francoise" uniqKey="D Aloisio F" first="Françoise" last="D'Aloisio">Françoise D'Aloisio</name><name sortKey="Gobet, Francoise" sort="Gobet, Francoise" uniqKey="Gobet F" first="Françoise" last="Gobet">Françoise Gobet</name><name sortKey="Maillet, Geraldine" sort="Maillet, Geraldine" uniqKey="Maillet G" first="Geraldine" last="Maillet">Geraldine Maillet</name><name sortKey="Minier, Christophe" sort="Minier, Christophe" uniqKey="Minier C" first="Christophe" last="Minier">Christophe Minier</name><name sortKey="Pfister, Christian" sort="Pfister, Christian" uniqKey="Pfister C" first="Christian" last="Pfister">Christian Pfister</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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