Seasonal variations in antioxidant defences in blue mussels Mytilus edulis collected from a polluted area: major contributions in gills of an inducible isoform of Cu/Zn-superoxide dismutase and of glutathione S-transferase.
Identifieur interne : 000114 ( Ncbi/Merge ); précédent : 000113; suivant : 000115Seasonal variations in antioxidant defences in blue mussels Mytilus edulis collected from a polluted area: major contributions in gills of an inducible isoform of Cu/Zn-superoxide dismutase and of glutathione S-transferase.
Auteurs : Hélène Manduzio [France] ; Tiphaine Monsinjon ; Camille Galap ; François Leboulenger ; Béatrice RocherSource :
- Aquatic toxicology (Amsterdam, Netherlands) [ 0166-445X ] ; 2004.
English descriptors
- KwdEn :
- Analysis of Variance, Animals, Antioxidants (metabolism), Bivalvia (enzymology), Environmental Monitoring (methods), Environmental Pollution, Gills (drug effects), Gills (enzymology), Glutathione Transferase (drug effects), Glutathione Transferase (metabolism), Isoenzymes (drug effects), Isoenzymes (metabolism), Oxidative Stress (physiology), Seasons, Superoxide Dismutase (drug effects), Superoxide Dismutase (metabolism).
- MESH :
- chemical , drug effects : Glutathione Transferase, Isoenzymes, Superoxide Dismutase.
- chemical , metabolism : Antioxidants, Glutathione Transferase, Isoenzymes, Superoxide Dismutase.
- drug effects : Gills.
- enzymology : Bivalvia, Gills.
- methods : Environmental Monitoring.
- physiology : Oxidative Stress.
- Analysis of Variance, Animals, Environmental Pollution, Seasons.
Abstract
In the present work, we investigated in the blue mussel (Mytilus edulis) the seasonal variations in the activity of several enzymes, which participate in the cellular defence system that is involved in the adaptive response of organisms to pollution. The activity levels of glutathione S-transferase, glutathione peroxidase, glutathione reductase and three isoforms of Cu/Zn-superoxide dismutase in gills and digestive glands of this bivalve species were used as biomarkers. Adult wild mussels were collected in Le Havre harbour (north-west coast of France) from four sites with different environmental conditions. Measurements of enzymatic activities were performed on tissue homogenates except for Cu/Zn-superoxide dismutase for which the activity of each isoform was detected on gel after isoelectric focusing. Seasonal variations in antioxidant enzyme activities were observed, characterized by low activity levels during winter, a period where oxidative stress is known to be high in bivalves. A clear-cut discrepancy between tissues was noted concerning inter-individual variability of data, which was low in gills but high in digestive gland, leading to the conclusion that gills could preferentially be used in biomonitoring studies dealing with oxidative stress in the blue mussel. As compared to animals from the reference site, mussels from the most polluted sites exhibited changes in the Cu/Zn-superoxide dismutase pattern characterized by an increase in the activity of the more acidic isoform without significant variation of the total activity of the enzyme. The most striking data were recorded in mussels collected at the outlet of a thermoelectric power plant. When compared to animals from the reference site, not only their gills showed a highly significant induction of the most acidic isoform of the Cu/Zn-superoxide dismutase (+340%, P < 0.001) but also high levels of glutathione S-transferase activity (+269%, P < 0.001). This study points out the usefulness of Cu/Zn-superoxide dismutase expression pattern as a biomarker of exposure to environmental stress rather than measurement of total activity of the enzyme, in field studies using Mytilus edulis. It also indicates the informative potential for glutathione S-transferase measurements in gills and underlines the advantages of selecting a battery of biomarkers for evaluating the impact of contamination on marine organisms.
DOI: 10.1016/j.aquatox.2004.07.003
PubMed: 15451609
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: 000319
- to stream PubMed, to step Curation: 000319
- to stream PubMed, to step Checkpoint: 000319
Links to Exploration step
pubmed:15451609Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Seasonal variations in antioxidant defences in blue mussels Mytilus edulis collected from a polluted area: major contributions in gills of an inducible isoform of Cu/Zn-superoxide dismutase and of glutathione S-transferase.</title><author><name sortKey="Manduzio, Helene" sort="Manduzio, Helene" uniqKey="Manduzio H" first="Hélène" last="Manduzio">Hélène Manduzio</name><affiliation wicri:level="3"><nlm:affiliation>Laboratory of Ecotoxicology (LEMA), UPRES-EA 3222, IFRMP 23, UFR of Sciences and Techniques, University of Le Havre, 25 rue Philippe Lebon-BP 540, 76058 Le Havre Cedex, France. helene.manduzio@univ-lehavre.fr</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Laboratory of Ecotoxicology (LEMA), UPRES-EA 3222, IFRMP 23, UFR of Sciences and Techniques, University of Le Havre, 25 rue Philippe Lebon-BP 540, 76058 Le Havre Cedex</wicri:regionArea><placeName><region type="region" nuts="2">Région Normandie</region><region type="old region" nuts="2">Haute-Normandie</region><settlement type="city">Le Havre</settlement></placeName></affiliation></author><author><name sortKey="Monsinjon, Tiphaine" sort="Monsinjon, Tiphaine" uniqKey="Monsinjon T" first="Tiphaine" last="Monsinjon">Tiphaine Monsinjon</name></author><author><name sortKey="Galap, Camille" sort="Galap, Camille" uniqKey="Galap C" first="Camille" last="Galap">Camille Galap</name></author><author><name sortKey="Leboulenger, Francois" sort="Leboulenger, Francois" uniqKey="Leboulenger F" first="François" last="Leboulenger">François Leboulenger</name></author><author><name sortKey="Rocher, Beatrice" sort="Rocher, Beatrice" uniqKey="Rocher B" first="Béatrice" last="Rocher">Béatrice Rocher</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2004">2004</date><idno type="RBID">pubmed:15451609</idno><idno type="pmid">15451609</idno><idno type="doi">10.1016/j.aquatox.2004.07.003</idno><idno type="wicri:Area/PubMed/Corpus">000319</idno><idno type="wicri:Area/PubMed/Curation">000319</idno><idno type="wicri:Area/PubMed/Checkpoint">000319</idno><idno type="wicri:Area/Ncbi/Merge">000114</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Seasonal variations in antioxidant defences in blue mussels Mytilus edulis collected from a polluted area: major contributions in gills of an inducible isoform of Cu/Zn-superoxide dismutase and of glutathione S-transferase.</title><author><name sortKey="Manduzio, Helene" sort="Manduzio, Helene" uniqKey="Manduzio H" first="Hélène" last="Manduzio">Hélène Manduzio</name><affiliation wicri:level="3"><nlm:affiliation>Laboratory of Ecotoxicology (LEMA), UPRES-EA 3222, IFRMP 23, UFR of Sciences and Techniques, University of Le Havre, 25 rue Philippe Lebon-BP 540, 76058 Le Havre Cedex, France. helene.manduzio@univ-lehavre.fr</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Laboratory of Ecotoxicology (LEMA), UPRES-EA 3222, IFRMP 23, UFR of Sciences and Techniques, University of Le Havre, 25 rue Philippe Lebon-BP 540, 76058 Le Havre Cedex</wicri:regionArea><placeName><region type="region" nuts="2">Région Normandie</region><region type="old region" nuts="2">Haute-Normandie</region><settlement type="city">Le Havre</settlement></placeName></affiliation></author><author><name sortKey="Monsinjon, Tiphaine" sort="Monsinjon, Tiphaine" uniqKey="Monsinjon T" first="Tiphaine" last="Monsinjon">Tiphaine Monsinjon</name></author><author><name sortKey="Galap, Camille" sort="Galap, Camille" uniqKey="Galap C" first="Camille" last="Galap">Camille Galap</name></author><author><name sortKey="Leboulenger, Francois" sort="Leboulenger, Francois" uniqKey="Leboulenger F" first="François" last="Leboulenger">François Leboulenger</name></author><author><name sortKey="Rocher, Beatrice" sort="Rocher, Beatrice" uniqKey="Rocher B" first="Béatrice" last="Rocher">Béatrice Rocher</name></author></analytic><series><title level="j">Aquatic toxicology (Amsterdam, Netherlands)</title><idno type="ISSN">0166-445X</idno><imprint><date when="2004" type="published">2004</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Analysis of Variance</term><term>Animals</term><term>Antioxidants (metabolism)</term><term>Bivalvia (enzymology)</term><term>Environmental Monitoring (methods)</term><term>Environmental Pollution</term><term>Gills (drug effects)</term><term>Gills (enzymology)</term><term>Glutathione Transferase (drug effects)</term><term>Glutathione Transferase (metabolism)</term><term>Isoenzymes (drug effects)</term><term>Isoenzymes (metabolism)</term><term>Oxidative Stress (physiology)</term><term>Seasons</term><term>Superoxide Dismutase (drug effects)</term><term>Superoxide Dismutase (metabolism)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="drug effects" xml:lang="en"><term>Glutathione Transferase</term><term>Isoenzymes</term><term>Superoxide Dismutase</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Antioxidants</term><term>Glutathione Transferase</term><term>Isoenzymes</term><term>Superoxide Dismutase</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Gills</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Bivalvia</term><term>Gills</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Environmental Monitoring</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Oxidative Stress</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Analysis of Variance</term><term>Animals</term><term>Environmental Pollution</term><term>Seasons</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">In the present work, we investigated in the blue mussel (Mytilus edulis) the seasonal variations in the activity of several enzymes, which participate in the cellular defence system that is involved in the adaptive response of organisms to pollution. The activity levels of glutathione S-transferase, glutathione peroxidase, glutathione reductase and three isoforms of Cu/Zn-superoxide dismutase in gills and digestive glands of this bivalve species were used as biomarkers. Adult wild mussels were collected in Le Havre harbour (north-west coast of France) from four sites with different environmental conditions. Measurements of enzymatic activities were performed on tissue homogenates except for Cu/Zn-superoxide dismutase for which the activity of each isoform was detected on gel after isoelectric focusing. Seasonal variations in antioxidant enzyme activities were observed, characterized by low activity levels during winter, a period where oxidative stress is known to be high in bivalves. A clear-cut discrepancy between tissues was noted concerning inter-individual variability of data, which was low in gills but high in digestive gland, leading to the conclusion that gills could preferentially be used in biomonitoring studies dealing with oxidative stress in the blue mussel. As compared to animals from the reference site, mussels from the most polluted sites exhibited changes in the Cu/Zn-superoxide dismutase pattern characterized by an increase in the activity of the more acidic isoform without significant variation of the total activity of the enzyme. The most striking data were recorded in mussels collected at the outlet of a thermoelectric power plant. When compared to animals from the reference site, not only their gills showed a highly significant induction of the most acidic isoform of the Cu/Zn-superoxide dismutase (+340%, P < 0.001) but also high levels of glutathione S-transferase activity (+269%, P < 0.001). This study points out the usefulness of Cu/Zn-superoxide dismutase expression pattern as a biomarker of exposure to environmental stress rather than measurement of total activity of the enzyme, in field studies using Mytilus edulis. It also indicates the informative potential for glutathione S-transferase measurements in gills and underlines the advantages of selecting a battery of biomarkers for evaluating the impact of contamination on marine organisms.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">15451609</PMID><DateCreated><Year>2004</Year><Month>9</Month><Day>28</Day></DateCreated><DateCompleted><Year>2004</Year><Month>12</Month><Day>21</Day></DateCompleted><DateRevised><Year>2006</Year><Month>11</Month><Day>15</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0166-445X</ISSN><JournalIssue CitedMedium="Print"><Volume>70</Volume><Issue>1</Issue><PubDate><Year>2004</Year><Month>Oct</Month><Day>18</Day></PubDate></JournalIssue><Title>Aquatic toxicology (Amsterdam, Netherlands)</Title><ISOAbbreviation>Aquat. Toxicol.</ISOAbbreviation></Journal><ArticleTitle>Seasonal variations in antioxidant defences in blue mussels Mytilus edulis collected from a polluted area: major contributions in gills of an inducible isoform of Cu/Zn-superoxide dismutase and of glutathione S-transferase.</ArticleTitle><Pagination><MedlinePgn>83-93</MedlinePgn></Pagination><Abstract><AbstractText>In the present work, we investigated in the blue mussel (Mytilus edulis) the seasonal variations in the activity of several enzymes, which participate in the cellular defence system that is involved in the adaptive response of organisms to pollution. The activity levels of glutathione S-transferase, glutathione peroxidase, glutathione reductase and three isoforms of Cu/Zn-superoxide dismutase in gills and digestive glands of this bivalve species were used as biomarkers. Adult wild mussels were collected in Le Havre harbour (north-west coast of France) from four sites with different environmental conditions. Measurements of enzymatic activities were performed on tissue homogenates except for Cu/Zn-superoxide dismutase for which the activity of each isoform was detected on gel after isoelectric focusing. Seasonal variations in antioxidant enzyme activities were observed, characterized by low activity levels during winter, a period where oxidative stress is known to be high in bivalves. A clear-cut discrepancy between tissues was noted concerning inter-individual variability of data, which was low in gills but high in digestive gland, leading to the conclusion that gills could preferentially be used in biomonitoring studies dealing with oxidative stress in the blue mussel. As compared to animals from the reference site, mussels from the most polluted sites exhibited changes in the Cu/Zn-superoxide dismutase pattern characterized by an increase in the activity of the more acidic isoform without significant variation of the total activity of the enzyme. The most striking data were recorded in mussels collected at the outlet of a thermoelectric power plant. When compared to animals from the reference site, not only their gills showed a highly significant induction of the most acidic isoform of the Cu/Zn-superoxide dismutase (+340%, P < 0.001) but also high levels of glutathione S-transferase activity (+269%, P < 0.001). This study points out the usefulness of Cu/Zn-superoxide dismutase expression pattern as a biomarker of exposure to environmental stress rather than measurement of total activity of the enzyme, in field studies using Mytilus edulis. It also indicates the informative potential for glutathione S-transferase measurements in gills and underlines the advantages of selecting a battery of biomarkers for evaluating the impact of contamination on marine organisms.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Manduzio</LastName><ForeName>Hélène</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>Laboratory of Ecotoxicology (LEMA), UPRES-EA 3222, IFRMP 23, UFR of Sciences and Techniques, University of Le Havre, 25 rue Philippe Lebon-BP 540, 76058 Le Havre Cedex, France. helene.manduzio@univ-lehavre.fr</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Monsinjon</LastName><ForeName>Tiphaine</ForeName><Initials>T</Initials></Author><Author ValidYN="Y"><LastName>Galap</LastName><ForeName>Camille</ForeName><Initials>C</Initials></Author><Author ValidYN="Y"><LastName>Leboulenger</LastName><ForeName>François</ForeName><Initials>F</Initials></Author><Author ValidYN="Y"><LastName>Rocher</LastName><ForeName>Béatrice</ForeName><Initials>B</Initials></Author></AuthorList><Language>ENG</Language><PublicationTypeList><PublicationType UI="D003160">Comparative Study</PublicationType><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>Netherlands</Country><MedlineTA>Aquat Toxicol</MedlineTA><NlmUniqueID>8500246</NlmUniqueID><ISSNLinking>0166-445X</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000975">Antioxidants</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D007527">Isoenzymes</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 1.15.1.1</RegistryNumber><NameOfSubstance UI="D013482">Superoxide Dismutase</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 2.5.1.18</RegistryNumber><NameOfSubstance UI="D005982">Glutathione Transferase</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000704" MajorTopicYN="N">Analysis of Variance</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000975" MajorTopicYN="N">Antioxidants</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D049872" MajorTopicYN="N">Bivalvia</DescriptorName><QualifierName UI="Q000201" MajorTopicYN="Y">enzymology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004784" MajorTopicYN="N">Environmental Monitoring</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="N">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004787" MajorTopicYN="N">Environmental Pollution</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005880" MajorTopicYN="N">Gills</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName><QualifierName UI="Q000201" MajorTopicYN="Y">enzymology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005982" MajorTopicYN="N">Glutathione Transferase</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D007527" MajorTopicYN="N">Isoenzymes</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018384" MajorTopicYN="N">Oxidative Stress</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012621" MajorTopicYN="Y">Seasons</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D013482" MajorTopicYN="N">Superoxide Dismutase</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2003</Year><Month>Sep</Month><Day>11</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2004</Year><Month>Jun</Month><Day>4</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2004</Year><Month>Jul</Month><Day>20</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2004</Year><Month>9</Month><Day>29</Day><Hour>5</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2004</Year><Month>12</Month><Day>22</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2004</Year><Month>9</Month><Day>29</Day><Hour>5</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">15451609</ArticleId><ArticleId IdType="doi">10.1016/j.aquatox.2004.07.003</ArticleId><ArticleId IdType="pii">S0166-445X(04)00226-7</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>France</li></country><region><li>Haute-Normandie</li><li>Région Normandie</li></region><settlement><li>Le Havre</li></settlement></list><tree><noCountry><name sortKey="Galap, Camille" sort="Galap, Camille" uniqKey="Galap C" first="Camille" last="Galap">Camille Galap</name><name sortKey="Leboulenger, Francois" sort="Leboulenger, Francois" uniqKey="Leboulenger F" first="François" last="Leboulenger">François Leboulenger</name><name sortKey="Monsinjon, Tiphaine" sort="Monsinjon, Tiphaine" uniqKey="Monsinjon T" first="Tiphaine" last="Monsinjon">Tiphaine Monsinjon</name><name sortKey="Rocher, Beatrice" sort="Rocher, Beatrice" uniqKey="Rocher B" first="Béatrice" last="Rocher">Béatrice Rocher</name></noCountry><country name="France"><region name="Région Normandie"><name sortKey="Manduzio, Helene" sort="Manduzio, Helene" uniqKey="Manduzio H" first="Hélène" last="Manduzio">Hélène Manduzio</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/France/explor/LeHavreV1/Data/Ncbi/Merge
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000114 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Ncbi/Merge/biblio.hfd -nk 000114 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/France
|area= LeHavreV1
|flux= Ncbi
|étape= Merge
|type= RBID
|clé= pubmed:15451609
|texte= Seasonal variations in antioxidant defences in blue mussels Mytilus edulis collected from a polluted area: major contributions in gills of an inducible isoform of Cu/Zn-superoxide dismutase and of glutathione S-transferase.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Ncbi/Merge/RBID.i -Sk "pubmed:15451609" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Ncbi/Merge/biblio.hfd \
| NlmPubMed2Wicri -a LeHavreV1
| This area was generated with Dilib version V0.6.25. |  |