New Real-Time PCR Assay Using Locked Nucleic Acid Probes To Assess Prevalence of ParC Mutations in Fluoroquinolone-Susceptible Streptococcus pneumoniae Isolates from France
Identifieur interne : 000148 ( Ncbi/Curation ); précédent : 000147; suivant : 000149New Real-Time PCR Assay Using Locked Nucleic Acid Probes To Assess Prevalence of ParC Mutations in Fluoroquinolone-Susceptible Streptococcus pneumoniae Isolates from France
Auteurs : Jean-Winoc Decousser ; Imen Methlouthi ; Patrick Pina ; Anne Collignon ; Pierre AllouchSource :
- Antimicrobial Agents and Chemotherapy [ 0066-4804 ] ; 2006.
Abstract
A real-time PCR assay with locked nucleic acid probes was developed to screen mutations at codons 79 and 83 of the
Url:
DOI: 10.1128/AAC.50.4.1594-1598.2006
PubMed: 16569894
PubMed Central: 1426917
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PMC:1426917Le document en format XML
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Isolates from France</title>
<author><name sortKey="Decousser, Jean Winoc" sort="Decousser, Jean Winoc" uniqKey="Decousser J" first="Jean-Winoc" last="Decousser">Jean-Winoc Decousser</name>
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<author><name sortKey="Collignon, Anne" sort="Collignon, Anne" uniqKey="Collignon A" first="Anne" last="Collignon">Anne Collignon</name>
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<author><name sortKey="Allouch, Pierre" sort="Allouch, Pierre" uniqKey="Allouch P" first="Pierre" last="Allouch">Pierre Allouch</name>
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<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">New Real-Time PCR Assay Using Locked Nucleic Acid Probes To Assess Prevalence of ParC Mutations in Fluoroquinolone-Susceptible <italic>Streptococcus pneumoniae</italic>
Isolates from France</title>
<author><name sortKey="Decousser, Jean Winoc" sort="Decousser, Jean Winoc" uniqKey="Decousser J" first="Jean-Winoc" last="Decousser">Jean-Winoc Decousser</name>
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<author><name sortKey="Pina, Patrick" sort="Pina, Patrick" uniqKey="Pina P" first="Patrick" last="Pina">Patrick Pina</name>
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<author><name sortKey="Collignon, Anne" sort="Collignon, Anne" uniqKey="Collignon A" first="Anne" last="Collignon">Anne Collignon</name>
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<author><name sortKey="Allouch, Pierre" sort="Allouch, Pierre" uniqKey="Allouch P" first="Pierre" last="Allouch">Pierre Allouch</name>
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<series><title level="j">Antimicrobial Agents and Chemotherapy</title>
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<front><div type="abstract" xml:lang="en"><p>A real-time PCR assay with locked nucleic acid probes was developed to screen mutations at codons 79 and 83 of the <italic>Streptococcus pneumoniae parC</italic>
gene. Only silent mutations were detected among 236 French invasive fluoroquinolone-susceptible strains. This test could be useful for some high-risk patients or in national surveys.</p>
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</front>
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