Determination of thiocyanate in plasma by ion chromatography and ultraviolet detection.
Identifieur interne : 000F31 ( Main/Merge ); précédent : 000F30; suivant : 000F32Determination of thiocyanate in plasma by ion chromatography and ultraviolet detection.
Auteurs : Elodie Saussereau [France] ; Jean-Pierre Goullé ; Christian LacroixSource :
- Journal of analytical toxicology [ 0146-4760 ] ; 2007.
English descriptors
- KwdEn :
- MESH :
- chemical , blood : Thiocyanates.
- methods : Chromatography, Ion Exchange, Spectrophotometry, Ultraviolet.
- Autopsy, Fires, Forensic Medicine, Humans.
Abstract
A specific and sensitive rapid high-performance liquid chromatographic method has been developed for determination of thiocyanate (SCN-) in plasma. This method is based on anion exchange chromatography after a simple ultrafiltration of plasma diluted in water. The detection of SCN- is carried out in ultraviolet (lambda = 210 nm). The proposed method is linear in the range 1-30 mg/L. Intra-assay and interassay accuracy and precision were maintained within the designated limit (< 20%). The total recovery of SCN- varied between 97 and 103.9%. The method described should be useful for clinical medicine. Moreover, this method was applied to the analysis of SCN- plasma in deceased subjects, within the context of fire, and could be of interest in forensic science as a useful additional measurement tool for cyanide determination in blood.
PubMed: 17725886
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pubmed:17725886Le document en format XML
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<author><name sortKey="Saussereau, Elodie" sort="Saussereau, Elodie" uniqKey="Saussereau E" first="Elodie" last="Saussereau">Elodie Saussereau</name>
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<author><name sortKey="Goulle, Jean Pierre" sort="Goulle, Jean Pierre" uniqKey="Goulle J" first="Jean-Pierre" last="Goullé">Jean-Pierre Goullé</name>
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<author><name sortKey="Lacroix, Christian" sort="Lacroix, Christian" uniqKey="Lacroix C" first="Christian" last="Lacroix">Christian Lacroix</name>
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<author><name sortKey="Goulle, Jean Pierre" sort="Goulle, Jean Pierre" uniqKey="Goulle J" first="Jean-Pierre" last="Goullé">Jean-Pierre Goullé</name>
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<series><title level="j">Journal of analytical toxicology</title>
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<imprint><date when="2007" type="published">2007</date>
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<term>Chromatography, Ion Exchange (methods)</term>
<term>Fires</term>
<term>Forensic Medicine</term>
<term>Humans</term>
<term>Spectrophotometry, Ultraviolet (methods)</term>
<term>Thiocyanates (blood)</term>
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<keywords scheme="MESH" type="chemical" qualifier="blood" xml:lang="en"><term>Thiocyanates</term>
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<keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Chromatography, Ion Exchange</term>
<term>Spectrophotometry, Ultraviolet</term>
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<keywords scheme="MESH" xml:lang="en"><term>Autopsy</term>
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<front><div type="abstract" xml:lang="en">A specific and sensitive rapid high-performance liquid chromatographic method has been developed for determination of thiocyanate (SCN-) in plasma. This method is based on anion exchange chromatography after a simple ultrafiltration of plasma diluted in water. The detection of SCN- is carried out in ultraviolet (lambda = 210 nm). The proposed method is linear in the range 1-30 mg/L. Intra-assay and interassay accuracy and precision were maintained within the designated limit (< 20%). The total recovery of SCN- varied between 97 and 103.9%. The method described should be useful for clinical medicine. Moreover, this method was applied to the analysis of SCN- plasma in deceased subjects, within the context of fire, and could be of interest in forensic science as a useful additional measurement tool for cyanide determination in blood.</div>
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