Evaluation of the genotoxicity of river sediments from industrialized and unaffected areas using a battery of short‐term bioassays
Identifieur interne : 000E15 ( Main/Exploration ); précédent : 000E14; suivant : 000E16Evaluation of the genotoxicity of river sediments from industrialized and unaffected areas using a battery of short‐term bioassays
Auteurs : Assia Aouadene [France] ; Carole Di Giorgio [France] ; Luc Sarrazin [France] ; Xavier Moreau [France] ; Laetitia De Jong [France] ; Fabrice Garcia [France] ; Alain Thiery [France] ; Alain Botta [France] ; Michel De Méo [France]Source :
- Environmental and Molecular Mutagenesis [ 0893-6692 ] ; 2008-05.
English descriptors
- KwdEn :
Abstract
The present investigation evaluated the capacity of the Salmonella mutagenicity test, the comet assay, and the micronucleus assay to detect and characterize the genotoxic profile of river sediments. Three stations were selected on an urban river (Bouches du Rhône, France) exposed to various sources of industrial and urban pollution (StA, StB, and StC) and one station on its tributary (StD). One station in a nonurban river was included (REF). The concentrations of 16 polycyclic aromatic hydrocarbons (PAHs) were determined by HPLC, and the genotoxicity of the sediments was monitored by the Salmonella mutagenicity test (TA98 + S9, YG1041 ± S9), the comet assay, and the micronucleus assay on CHO cells. Chemical analysis showed that the total PAH concentrations ranged from 23 μg kg−1 dw (REF) to 1285 μg kg−1 dw (StD). All the sediments were mutagenic in the Salmonella mutagenicity test. The mutagenicity was probably induced by the presence of nitroarenes (StA, StB, StC, and StD) and aromatic amines (REF) as deduced from the mutagenicity profiles of strains YG1041 ± S9 and TA98 + S9. The comet assay revealed direct DNA lesions in REF, StA, and StB sediments and metabolization‐dependent DNA damage in StC and StD. The micronucleus assay showed an absence of clastogenicity for StA ± S9 and StC‐S9, and a significant clastogenicity ± S9 for the three other stations. The genotoxicity ranking determined by the comet assay + S9 matched the ranking of total and carcinogenic PAH concentrations, and this assay was found to be the most sensitive. Environ. Mol. Mutagen., 2008. © 2008 Wiley‐Liss, Inc.
Url:
DOI: 10.1002/em.20384
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: 001413
- to stream Istex, to step Curation: 001413
- to stream Istex, to step Checkpoint: 000387
- to stream Main, to step Merge: 000E26
- to stream Main, to step Curation: 000E15
Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Evaluation of the genotoxicity of river sediments from industrialized and unaffected areas using a battery of short‐term bioassays</title><author><name sortKey="Aouadene, Assia" sort="Aouadene, Assia" uniqKey="Aouadene A" first="Assia" last="Aouadene">Assia Aouadene</name></author><author><name sortKey="Di Giorgio, Carole" sort="Di Giorgio, Carole" uniqKey="Di Giorgio C" first="Carole" last="Di Giorgio">Carole Di Giorgio</name></author><author><name sortKey="Sarrazin, Luc" sort="Sarrazin, Luc" uniqKey="Sarrazin L" first="Luc" last="Sarrazin">Luc Sarrazin</name></author><author><name sortKey="Moreau, Xavier" sort="Moreau, Xavier" uniqKey="Moreau X" first="Xavier" last="Moreau">Xavier Moreau</name></author><author><name sortKey="De Jong, Laetitia" sort="De Jong, Laetitia" uniqKey="De Jong L" first="Laetitia" last="De Jong">Laetitia De Jong</name></author><author><name sortKey="Garcia, Fabrice" sort="Garcia, Fabrice" uniqKey="Garcia F" first="Fabrice" last="Garcia">Fabrice Garcia</name></author><author><name sortKey="Thiery, Alain" sort="Thiery, Alain" uniqKey="Thiery A" first="Alain" last="Thiery">Alain Thiery</name></author><author><name sortKey="Botta, Alain" sort="Botta, Alain" uniqKey="Botta A" first="Alain" last="Botta">Alain Botta</name></author><author><name sortKey="De Meo, Michel" sort="De Meo, Michel" uniqKey="De Meo M" first="Michel" last="De Méo">Michel De Méo</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:0A7349E858A1896C845739DD768E1F034CA0D4D1</idno><date when="2008" year="2008">2008</date><idno type="doi">10.1002/em.20384</idno><idno type="url">https://api.istex.fr/document/0A7349E858A1896C845739DD768E1F034CA0D4D1/fulltext/pdf</idno><idno type="wicri:Area/Istex/Corpus">001413</idno><idno type="wicri:Area/Istex/Curation">001413</idno><idno type="wicri:Area/Istex/Checkpoint">000387</idno><idno type="wicri:doubleKey">0893-6692:2008:Aouadene A:evaluation:of:the</idno><idno type="wicri:Area/Main/Merge">000E26</idno><idno type="wicri:Area/Main/Curation">000E15</idno><idno type="wicri:Area/Main/Exploration">000E15</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Evaluation of the genotoxicity of river sediments from industrialized and unaffected areas using a battery of short‐term bioassays</title><author><name sortKey="Aouadene, Assia" sort="Aouadene, Assia" uniqKey="Aouadene A" first="Assia" last="Aouadene">Assia Aouadene</name><affiliation wicri:level="4"><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire de Biogénotoxicologie et Mutagenèse Environnementale, Université de la Méditerranée, Facultés de Médecine et Pharmacie, Marseille</wicri:regionArea><placeName><settlement type="city">Marseille</settlement></placeName><orgName type="university">Université de la Méditerranée</orgName><placeName><settlement type="city">Marseille</settlement><region type="region" nuts="2">Provence-Alpes-Côte d'Azur</region></placeName></affiliation></author><author><name sortKey="Di Giorgio, Carole" sort="Di Giorgio, Carole" uniqKey="Di Giorgio C" first="Carole" last="Di Giorgio">Carole Di Giorgio</name><affiliation wicri:level="4"><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire de Biogénotoxicologie et Mutagenèse Environnementale, Université de la Méditerranée, Facultés de Médecine et Pharmacie, Marseille</wicri:regionArea><placeName><settlement type="city">Marseille</settlement></placeName><orgName type="university">Université de la Méditerranée</orgName><placeName><settlement type="city">Marseille</settlement><region type="region" nuts="2">Provence-Alpes-Côte d'Azur</region></placeName></affiliation></author><author><name sortKey="Sarrazin, Luc" sort="Sarrazin, Luc" uniqKey="Sarrazin L" first="Luc" last="Sarrazin">Luc Sarrazin</name><affiliation wicri:level="4"><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire d'Hydrologie et de Molysmologie Aquatique, Université de la Méditerranée, Faculté de Pharmacie, Marseille</wicri:regionArea><placeName><settlement type="city">Marseille</settlement></placeName><orgName type="university">Université de la Méditerranée</orgName><placeName><settlement type="city">Marseille</settlement><region type="region" nuts="2">Provence-Alpes-Côte d'Azur</region></placeName></affiliation></author><author><name sortKey="Moreau, Xavier" sort="Moreau, Xavier" uniqKey="Moreau X" first="Xavier" last="Moreau">Xavier Moreau</name><affiliation wicri:level="4"><country xml:lang="fr">France</country><wicri:regionArea>UMR‐Centre National de la Recherche Scientifique 6116 IMEP, Case 17, Université de Provence, Marseille</wicri:regionArea><placeName><settlement type="city">Marseille</settlement></placeName><orgName type="university">Université de Provence</orgName><placeName><settlement type="city">Marseille</settlement><region type="region" nuts="2">Provence-Alpes-Côte d'Azur</region></placeName></affiliation></author><author><name sortKey="De Jong, Laetitia" sort="De Jong, Laetitia" uniqKey="De Jong L" first="Laetitia" last="De Jong">Laetitia De Jong</name><affiliation wicri:level="4"><country xml:lang="fr">France</country><wicri:regionArea>UMR‐Centre National de la Recherche Scientifique 6116 IMEP, Case 17, Université de Provence, Marseille</wicri:regionArea><placeName><settlement type="city">Marseille</settlement></placeName><orgName type="university">Université de Provence</orgName><placeName><settlement type="city">Marseille</settlement><region type="region" nuts="2">Provence-Alpes-Côte d'Azur</region></placeName></affiliation></author><author><name sortKey="Garcia, Fabrice" sort="Garcia, Fabrice" uniqKey="Garcia F" first="Fabrice" last="Garcia">Fabrice Garcia</name><affiliation wicri:level="4"><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire d'Hydrologie et de Molysmologie Aquatique, Université de la Méditerranée, Faculté de Pharmacie, Marseille</wicri:regionArea><placeName><settlement type="city">Marseille</settlement></placeName><orgName type="university">Université de la Méditerranée</orgName><placeName><settlement type="city">Marseille</settlement><region type="region" nuts="2">Provence-Alpes-Côte d'Azur</region></placeName></affiliation></author><author><name sortKey="Thiery, Alain" sort="Thiery, Alain" uniqKey="Thiery A" first="Alain" last="Thiery">Alain Thiery</name><affiliation wicri:level="4"><country xml:lang="fr">France</country><wicri:regionArea>UMR‐Centre National de la Recherche Scientifique 6116 IMEP, Case 17, Université de Provence, Marseille</wicri:regionArea><placeName><settlement type="city">Marseille</settlement></placeName><orgName type="university">Université de Provence</orgName><placeName><settlement type="city">Marseille</settlement><region type="region" nuts="2">Provence-Alpes-Côte d'Azur</region></placeName></affiliation></author><author><name sortKey="Botta, Alain" sort="Botta, Alain" uniqKey="Botta A" first="Alain" last="Botta">Alain Botta</name><affiliation wicri:level="4"><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire de Biogénotoxicologie et Mutagenèse Environnementale, Université de la Méditerranée, Facultés de Médecine et Pharmacie, Marseille</wicri:regionArea><placeName><settlement type="city">Marseille</settlement></placeName><orgName type="university">Université de la Méditerranée</orgName><placeName><settlement type="city">Marseille</settlement><region type="region" nuts="2">Provence-Alpes-Côte d'Azur</region></placeName></affiliation></author><author><name sortKey="De Meo, Michel" sort="De Meo, Michel" uniqKey="De Meo M" first="Michel" last="De Méo">Michel De Méo</name><affiliation wicri:level="4"><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire de Biogénotoxicologie et Mutagenèse Environnementale, Université de la Méditerranée, Facultés de Médecine et Pharmacie, Marseille</wicri:regionArea><placeName><settlement type="city">Marseille</settlement></placeName><orgName type="university">Université de la Méditerranée</orgName><placeName><settlement type="city">Marseille</settlement><region type="region" nuts="2">Provence-Alpes-Côte d'Azur</region></placeName></affiliation></author></analytic><monogr></monogr><series><title level="j">Environmental and Molecular Mutagenesis</title><title level="j" type="abbrev">Environ. Mol. Mutagen.</title><idno type="ISSN">0893-6692</idno><idno type="eISSN">1098-2280</idno><imprint><publisher>Wiley Subscription Services, Inc., A Wiley Company</publisher><pubPlace>Hoboken</pubPlace><date type="published" when="2008-05">2008-05</date><biblScope unit="volume">49</biblScope><biblScope unit="issue">4</biblScope><biblScope unit="page" from="283">283</biblScope><biblScope unit="page" to="299">299</biblScope></imprint><idno type="ISSN">0893-6692</idno></series><idno type="istex">0A7349E858A1896C845739DD768E1F034CA0D4D1</idno><idno type="DOI">10.1002/em.20384</idno><idno type="ArticleID">EM20384</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0893-6692</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>PAHs</term><term>Salmonella mutagenicity test</term><term>comet assay</term><term>genotoxicity</term><term>micronucleus assay</term><term>pollution</term><term>river</term><term>sediment</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The present investigation evaluated the capacity of the Salmonella mutagenicity test, the comet assay, and the micronucleus assay to detect and characterize the genotoxic profile of river sediments. Three stations were selected on an urban river (Bouches du Rhône, France) exposed to various sources of industrial and urban pollution (StA, StB, and StC) and one station on its tributary (StD). One station in a nonurban river was included (REF). The concentrations of 16 polycyclic aromatic hydrocarbons (PAHs) were determined by HPLC, and the genotoxicity of the sediments was monitored by the Salmonella mutagenicity test (TA98 + S9, YG1041 ± S9), the comet assay, and the micronucleus assay on CHO cells. Chemical analysis showed that the total PAH concentrations ranged from 23 μg kg−1 dw (REF) to 1285 μg kg−1 dw (StD). All the sediments were mutagenic in the Salmonella mutagenicity test. The mutagenicity was probably induced by the presence of nitroarenes (StA, StB, StC, and StD) and aromatic amines (REF) as deduced from the mutagenicity profiles of strains YG1041 ± S9 and TA98 + S9. The comet assay revealed direct DNA lesions in REF, StA, and StB sediments and metabolization‐dependent DNA damage in StC and StD. The micronucleus assay showed an absence of clastogenicity for StA ± S9 and StC‐S9, and a significant clastogenicity ± S9 for the three other stations. The genotoxicity ranking determined by the comet assay + S9 matched the ranking of total and carcinogenic PAH concentrations, and this assay was found to be the most sensitive. Environ. Mol. Mutagen., 2008. © 2008 Wiley‐Liss, Inc.</div></front></TEI><affiliations><list><country><li>France</li></country><region><li>Provence-Alpes-Côte d'Azur</li></region><settlement><li>Marseille</li></settlement><orgName><li>Université de Provence</li><li>Université de la Méditerranée</li></orgName></list><tree><country name="France"><noRegion><name sortKey="Aouadene, Assia" sort="Aouadene, Assia" uniqKey="Aouadene A" first="Assia" last="Aouadene">Assia Aouadene</name></noRegion><name sortKey="Botta, Alain" sort="Botta, Alain" uniqKey="Botta A" first="Alain" last="Botta">Alain Botta</name><name sortKey="De Jong, Laetitia" sort="De Jong, Laetitia" uniqKey="De Jong L" first="Laetitia" last="De Jong">Laetitia De Jong</name><name sortKey="De Meo, Michel" sort="De Meo, Michel" uniqKey="De Meo M" first="Michel" last="De Méo">Michel De Méo</name><name sortKey="Di Giorgio, Carole" sort="Di Giorgio, Carole" uniqKey="Di Giorgio C" first="Carole" last="Di Giorgio">Carole Di Giorgio</name><name sortKey="Garcia, Fabrice" sort="Garcia, Fabrice" uniqKey="Garcia F" first="Fabrice" last="Garcia">Fabrice Garcia</name><name sortKey="Moreau, Xavier" sort="Moreau, Xavier" uniqKey="Moreau X" first="Xavier" last="Moreau">Xavier Moreau</name><name sortKey="Sarrazin, Luc" sort="Sarrazin, Luc" uniqKey="Sarrazin L" first="Luc" last="Sarrazin">Luc Sarrazin</name><name sortKey="Thiery, Alain" sort="Thiery, Alain" uniqKey="Thiery A" first="Alain" last="Thiery">Alain Thiery</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/France/explor/LeHavreV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000E15 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000E15 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/France
|area= LeHavreV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= ISTEX:0A7349E858A1896C845739DD768E1F034CA0D4D1
|texte= Evaluation of the genotoxicity of river sediments from industrialized and unaffected areas using a battery of short‐term bioassays
}}
| This area was generated with Dilib version V0.6.25. |  |