Microdosage de l'isoniazide et de l'acetylisoniazide plasmatiques par chromatographie liquide à haute performance
Identifieur interne : 000209 ( Istex/Curation ); précédent : 000208; suivant : 000210Microdosage de l'isoniazide et de l'acetylisoniazide plasmatiques par chromatographie liquide à haute performance
Auteurs : C. Lacroix [France] ; G. Laine [France] ; J. P. Goulle [France] ; J. Nouveau [France]Source :
- Journal of Chromatography B: Biomedical Sciences and Applications [ 0378-4347 ] ; 1984.
Abstract
Abstract: Determination of isoniazid and acetylisoniazid in plasma or serum by high-performance liquid chromatographyA method for the analysis of iosoniazid and acetylisoniazid in plasma or serum has been developed. This report describes a simple, quick and economically optimized reversed-phase high-performance liquid chromatographic assay using a small sample size without solvent extraction step. Isoniazid was condensed with cinnamic aldehyde after trichloroacetic acid deproteinisation. The method correlated well with a fluorimetric procedure. Precision, sensibility and accuracy were good. Common antituberculous drugs did not interfere.
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DOI: 10.1016/S0378-4347(00)84080-3
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<front><div type="abstract" xml:lang="en">Abstract: Determination of isoniazid and acetylisoniazid in plasma or serum by high-performance liquid chromatographyA method for the analysis of iosoniazid and acetylisoniazid in plasma or serum has been developed. This report describes a simple, quick and economically optimized reversed-phase high-performance liquid chromatographic assay using a small sample size without solvent extraction step. Isoniazid was condensed with cinnamic aldehyde after trichloroacetic acid deproteinisation. The method correlated well with a fluorimetric procedure. Precision, sensibility and accuracy were good. Common antituberculous drugs did not interfere.</div>
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