Characterization of Methylophaga species by rRNA Gene Restriction Patterns (Ribotyping)
Identifieur interne : 001175 ( France/Analysis ); précédent : 001174; suivant : 001176Characterization of Methylophaga species by rRNA Gene Restriction Patterns (Ribotyping)
Auteurs : Monique Janvier [France] ; Patrick A. D. Grimont [France] ; Francine Grimont [France]Source :
- Systematic and Applied Microbiology [ 0723-2020 ] ; 1999.
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Abstract
Summary: A total of 24 strains of Methylophaga, marine methylotrophic bacteria, were characterized by rRNA gene restriction patterns (ribotypes), after cleavage of total DNA with EcoRI or HindIII restriction endonuclease. Manual ribotyping and the RiboPrinter® Microbial Characterization System were used. The two genomic DNA groups could be differentiated each by ribotyping. Our study showed that ribotyping could characterize Methylophaga species and identify unknown isolates. All results were confirmed by DNA/DNA hybridization. Methylophaga sulfidovorans species was confirmed to be a third species by ribotyping and DNA/DNA hybridization.
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DOI: 10.1016/S0723-2020(99)80045-9
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Manual ribotyping and the RiboPrinter® Microbial Characterization System were used. The two genomic DNA groups could be differentiated each by ribotyping. Our study showed that ribotyping could characterize Methylophaga species and identify unknown isolates. All results were confirmed by DNA/DNA hybridization. Methylophaga sulfidovorans species was confirmed to be a third species by ribotyping and DNA/DNA hybridization.</div></front></TEI><affiliations><list><country><li>France</li></country><settlement><li>Paris</li></settlement></list><tree><country name="France"><noRegion><name sortKey="Janvier, Monique" sort="Janvier, Monique" uniqKey="Janvier M" first="Monique" last="Janvier">Monique Janvier</name></noRegion><name sortKey="Grimont, Francine" sort="Grimont, Francine" uniqKey="Grimont F" first="Francine" last="Grimont">Francine Grimont</name><name sortKey="Grimont, Patrick A D" sort="Grimont, Patrick A D" uniqKey="Grimont P" first="Patrick A. D." last="Grimont">Patrick A. D. 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