Regulation of volume-sensitive Cl- channels in multi-drug resistant MCF7 cells.
Identifieur interne : 000C90 ( France/Analysis ); précédent : 000C89; suivant : 000C91Regulation of volume-sensitive Cl- channels in multi-drug resistant MCF7 cells.
Auteurs : Matthieu Marin [France] ; Agnès Poret ; Géraldine Maillet ; François Leboulenger ; Frank Le FollSource :
- Biochemical and biophysical research communications [ 0006-291X ] ; 2005.
English descriptors
- KwdEn :
- MESH :
- chemical , metabolism : Chloride Channels, P-Glycoprotein.
- metabolism : Breast Neoplasms.
- pathology : Breast Neoplasms.
- Cell Line, Tumor, Cell Size, Drug Resistance, Multiple, Humans, Ion Channel Gating, Water-Electrolyte Balance.
Abstract
The P-glycoprotein (P-gp) is thought to be involved in the regulation of volume-sensitive chloride channels. In this study, the possible coupling between P-gp and swelling-activated chloride channels has been examined in MCF7 cells with sensitive (MDR-), resistant (MDR+), and reversed resistant (MDR(REV)) phenotypes. Western blot analysis showed that incubation of cells with doxorubicin induced P-gp expression in a reversible manner. Exposure of MDR+ cells to hypotonicity resulted in an inhibition of P-gp activity while hypotonic challenges induced swelling-activated chloride currents (I(Cl-swell)) in MDR-, MDR+, and MDR(REV) MCF7 cells. While verapamil inhibited I(Cl-swell) in all cell types, doxorubicin and vincristine rapidly and reversibly inhibited I(Cl-swell) uniquely in MDR+. Intracellular dialysis of MDR+ cells with C219 anti-P-gp antibody abolished the sensitivity of I(Cl-swell) to doxorubicin and led to a response pattern very close to that of MDR- cells. Taken together, these results strongly suggest that the P-glycoprotein regulates I(Cl-swell) in resistant MCF7.
DOI: 10.1016/j.bbrc.2005.07.010
PubMed: 16039989
Affiliations:
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pubmed:16039989Le document en format XML
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last="Poret">Agnès Poret</name></author><author><name sortKey="Maillet, Geraldine" sort="Maillet, Geraldine" uniqKey="Maillet G" first="Géraldine" last="Maillet">Géraldine Maillet</name></author><author><name sortKey="Leboulenger, Francois" sort="Leboulenger, Francois" uniqKey="Leboulenger F" first="François" last="Leboulenger">François Leboulenger</name></author><author><name sortKey="Le Foll, Frank" sort="Le Foll, Frank" uniqKey="Le Foll F" first="Frank" last="Le Foll">Frank Le Foll</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2005">2005</date><idno type="RBID">pubmed:16039989</idno><idno type="pmid">16039989</idno><idno type="doi">10.1016/j.bbrc.2005.07.010</idno><idno type="wicri:Area/PubMed/Corpus">000309</idno><idno type="wicri:Area/PubMed/Curation">000309</idno><idno type="wicri:Area/PubMed/Checkpoint">000309</idno><idno type="wicri:Area/Ncbi/Merge">000125</idno><idno type="wicri:Area/Ncbi/Curation">000125</idno><idno 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Lebon, 76058 Le Havre cedex</wicri:regionArea><placeName><region type="region" nuts="2">Région Normandie</region><region type="old region" nuts="2">Haute-Normandie</region><settlement type="city">Le Havre</settlement></placeName></affiliation></author><author><name sortKey="Poret, Agnes" sort="Poret, Agnes" uniqKey="Poret A" first="Agnès" last="Poret">Agnès Poret</name></author><author><name sortKey="Maillet, Geraldine" sort="Maillet, Geraldine" uniqKey="Maillet G" first="Géraldine" last="Maillet">Géraldine Maillet</name></author><author><name sortKey="Leboulenger, Francois" sort="Leboulenger, Francois" uniqKey="Leboulenger F" first="François" last="Leboulenger">François Leboulenger</name></author><author><name sortKey="Le Foll, Frank" sort="Le Foll, Frank" uniqKey="Le Foll F" first="Frank" last="Le Foll">Frank Le Foll</name></author></analytic><series><title level="j">Biochemical and biophysical research communications</title><idno type="ISSN">0006-291X</idno><imprint><date when="2005" type="published">2005</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Breast Neoplasms (metabolism)</term><term>Breast Neoplasms (pathology)</term><term>Cell Line, Tumor</term><term>Cell Size</term><term>Chloride Channels (metabolism)</term><term>Drug Resistance, Multiple</term><term>Humans</term><term>Ion Channel Gating</term><term>P-Glycoprotein (metabolism)</term><term>Water-Electrolyte Balance</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Chloride Channels</term><term>P-Glycoprotein</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Breast Neoplasms</term></keywords><keywords scheme="MESH" qualifier="pathology" xml:lang="en"><term>Breast Neoplasms</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Cell Line, Tumor</term><term>Cell Size</term><term>Drug Resistance, Multiple</term><term>Humans</term><term>Ion Channel Gating</term><term>Water-Electrolyte Balance</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The P-glycoprotein (P-gp) is thought to be involved in the regulation of volume-sensitive chloride channels. In this study, the possible coupling between P-gp and swelling-activated chloride channels has been examined in MCF7 cells with sensitive (MDR-), resistant (MDR+), and reversed resistant (MDR(REV)) phenotypes. Western blot analysis showed that incubation of cells with doxorubicin induced P-gp expression in a reversible manner. Exposure of MDR+ cells to hypotonicity resulted in an inhibition of P-gp activity while hypotonic challenges induced swelling-activated chloride currents (I(Cl-swell)) in MDR-, MDR+, and MDR(REV) MCF7 cells. While verapamil inhibited I(Cl-swell) in all cell types, doxorubicin and vincristine rapidly and reversibly inhibited I(Cl-swell) uniquely in MDR+. Intracellular dialysis of MDR+ cells with C219 anti-P-gp antibody abolished the sensitivity of I(Cl-swell) to doxorubicin and led to a response pattern very close to that of MDR- cells. Taken together, these results strongly suggest that the P-glycoprotein regulates I(Cl-swell) in resistant MCF7.</div></front></TEI><affiliations><list><country><li>France</li></country><region><li>Haute-Normandie</li><li>Région Normandie</li></region><settlement><li>Le Havre</li></settlement></list><tree><noCountry><name sortKey="Le Foll, Frank" sort="Le Foll, Frank" uniqKey="Le Foll F" first="Frank" last="Le Foll">Frank Le Foll</name><name sortKey="Leboulenger, Francois" sort="Leboulenger, Francois" uniqKey="Leboulenger F" first="François" last="Leboulenger">François Leboulenger</name><name sortKey="Maillet, Geraldine" sort="Maillet, Geraldine" uniqKey="Maillet G" first="Géraldine" last="Maillet">Géraldine Maillet</name><name sortKey="Poret, Agnes" sort="Poret, Agnes" uniqKey="Poret A" first="Agnès" last="Poret">Agnès Poret</name></noCountry><country name="France"><region name="Région Normandie"><name sortKey="Marin, Matthieu" sort="Marin, Matthieu" uniqKey="Marin M" first="Matthieu" last="Marin">Matthieu Marin</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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