Endothiovibrio diazotrophicus gen. nov., sp. nov., a novel nitrogen-fixing, sulfur-oxidizing gammaproteobacterium isolated from a salt marsh.
Identifieur interne : 000D35 ( PubMed/Corpus ); précédent : 000D34; suivant : 000D36Endothiovibrio diazotrophicus gen. nov., sp. nov., a novel nitrogen-fixing, sulfur-oxidizing gammaproteobacterium isolated from a salt marsh.
Auteurs : Dennis A. Bazylinski ; Viviana Morillo ; Christopher T. Lefèvre ; Nathan Viloria ; Bradley L. Dubbels ; Timothy J. WilliamsSource :
- International journal of systematic and evolutionary microbiology [ 1466-5034 ] ; 2017.
English descriptors
- KwdEn :
- Bacterial Typing Techniques, Base Composition, DNA, Bacterial (genetics), Fatty Acids (chemistry), Gammaproteobacteria (classification), Gammaproteobacteria (genetics), Gammaproteobacteria (isolation & purification), Massachusetts, Nitrogen (metabolism), Nitrogen Fixation, Phospholipids (chemistry), Phylogeny, RNA, Ribosomal, 16S (genetics), Sequence Analysis, DNA, Sulfur (metabolism), Water Microbiology, Wetlands.
- MESH :
- chemical , chemistry : Fatty Acids, Phospholipids.
- chemical , genetics : DNA, Bacterial, RNA, Ribosomal, 16S.
- classification : Gammaproteobacteria.
- genetics : Gammaproteobacteria.
- isolation & purification : Gammaproteobacteria.
- chemical , metabolism : Nitrogen, Sulfur.
- Bacterial Typing Techniques, Base Composition, Massachusetts, Nitrogen Fixation, Phylogeny, Sequence Analysis, DNA, Water Microbiology, Wetlands.
Abstract
A novel non-phototrophic, marine, sulfur-oxidizing bacterium, strain S-1T, was isolated from a coastal salt marsh in Massachusetts, USA. Cells are Gram-stain-negative vibrios motile by means of a single polar unsheathed flagellum. S-1T is an obligate microaerophile with limited metabolic capacity. It grows chemolithoautotrophically utilizing sulfide and thiosulfate as electron donors, converting these compounds to sulfate, and the Calvin-Benson-Bassham cycle for carbon fixation. Cells of S-1T did not grow on any of a large number of organic carbon sources and there was no evidence for chemoorganoheterotrophic growth. Cells produced internal sulfur globules during growth on sulfide and thiosulfate. S-1T is strongly diazotrophic, as demonstrated by 15N2 fixation and acetylene reduction activity by cells when a fixed nitrogen source is absent from the growth medium. The marine nature of this organism is evident from its ability to grow in 10 to 100 % artificial seawater but not at lower concentrations and NaCl alone cannot substitute for sea salts. The major cellular fatty acids are C16 : 1ω7c, C16 : 0, and C18 : 1ω7c. Phosphatidylethanolamine and phosphatidylglycerol are the major polar lipids. Q8 is the only respiratory quinone. S-1T genomic DNA has a G+C content of 67.6 mol%. Based on its 16S rRNA gene sequence, S-1T shows the closest phylogenetic relationship to non-phototrophic species within the family Thioalkalispiraceae of the class Gammaproteobacteria. The name Endothiovibrio diazotrophicus is proposed for this organism, with S-1T as the type strain (ATCC BAA-1439T=JCM 17961T).
DOI: 10.1099/ijsem.0.001743
PubMed: 27983471
Links to Exploration step
pubmed:27983471Le document en format XML
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Lefèvre</name><affiliation><nlm:affiliation>2CEA Cadarache/CNRS/Université Aix-Marseille, UMR7265 Biosciences and Biotechnologies Institute, Laboratoire de Bioénergétique Cellulaire, Saint Paul lez Durance 13108, France.</nlm:affiliation></affiliation></author><author><name sortKey="Viloria, Nathan" sort="Viloria, Nathan" uniqKey="Viloria N" first="Nathan" last="Viloria">Nathan Viloria</name><affiliation><nlm:affiliation>1School of Life Sciences, University of Nevada at Las Vegas, Las Vegas, Nevada 89154-4004, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Dubbels, Bradley L" sort="Dubbels, Bradley L" uniqKey="Dubbels B" first="Bradley L" last="Dubbels">Bradley L. Dubbels</name><affiliation><nlm:affiliation>3Novozymes North America Inc., 9000 Development Drive, Morrisville, North Carolina 27560, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Williams, Timothy J" sort="Williams, Timothy J" uniqKey="Williams T" first="Timothy J" last="Williams">Timothy J. Williams</name><affiliation><nlm:affiliation>4School of Biotechnology and Biomolecular Sciences, The University of New South Wales, Sydney NSW 2052, Australia.</nlm:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2017">2017</date><idno type="RBID">pubmed:27983471</idno><idno type="pmid">27983471</idno><idno type="doi">10.1099/ijsem.0.001743</idno><idno type="wicri:Area/PubMed/Corpus">000D35</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000D35</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Endothiovibrio diazotrophicus gen. nov., sp. nov., a novel nitrogen-fixing, sulfur-oxidizing gammaproteobacterium isolated from a salt marsh.</title><author><name sortKey="Bazylinski, Dennis A" sort="Bazylinski, Dennis A" uniqKey="Bazylinski D" first="Dennis A" last="Bazylinski">Dennis A. Bazylinski</name><affiliation><nlm:affiliation>1School of Life Sciences, University of Nevada at Las Vegas, Las Vegas, Nevada 89154-4004, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Morillo, Viviana" sort="Morillo, Viviana" uniqKey="Morillo V" first="Viviana" last="Morillo">Viviana Morillo</name><affiliation><nlm:affiliation>1School of Life Sciences, University of Nevada at Las Vegas, Las Vegas, Nevada 89154-4004, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Lefevre, Christopher T" sort="Lefevre, Christopher T" uniqKey="Lefevre C" first="Christopher T" last="Lefèvre">Christopher T. Lefèvre</name><affiliation><nlm:affiliation>2CEA Cadarache/CNRS/Université Aix-Marseille, UMR7265 Biosciences and Biotechnologies Institute, Laboratoire de Bioénergétique Cellulaire, Saint Paul lez Durance 13108, France.</nlm:affiliation></affiliation></author><author><name sortKey="Viloria, Nathan" sort="Viloria, Nathan" uniqKey="Viloria N" first="Nathan" last="Viloria">Nathan Viloria</name><affiliation><nlm:affiliation>1School of Life Sciences, University of Nevada at Las Vegas, Las Vegas, Nevada 89154-4004, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Dubbels, Bradley L" sort="Dubbels, Bradley L" uniqKey="Dubbels B" first="Bradley L" last="Dubbels">Bradley L. Dubbels</name><affiliation><nlm:affiliation>3Novozymes North America Inc., 9000 Development Drive, Morrisville, North Carolina 27560, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Williams, Timothy J" sort="Williams, Timothy J" uniqKey="Williams T" first="Timothy J" last="Williams">Timothy J. Williams</name><affiliation><nlm:affiliation>4School of Biotechnology and Biomolecular Sciences, The University of New South Wales, Sydney NSW 2052, Australia.</nlm:affiliation></affiliation></author></analytic><series><title level="j">International journal of systematic and evolutionary microbiology</title><idno type="eISSN">1466-5034</idno><imprint><date when="2017" type="published">2017</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Bacterial Typing Techniques</term><term>Base Composition</term><term>DNA, Bacterial (genetics)</term><term>Fatty Acids (chemistry)</term><term>Gammaproteobacteria (classification)</term><term>Gammaproteobacteria (genetics)</term><term>Gammaproteobacteria (isolation & purification)</term><term>Massachusetts</term><term>Nitrogen (metabolism)</term><term>Nitrogen Fixation</term><term>Phospholipids (chemistry)</term><term>Phylogeny</term><term>RNA, Ribosomal, 16S (genetics)</term><term>Sequence Analysis, DNA</term><term>Sulfur (metabolism)</term><term>Water Microbiology</term><term>Wetlands</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Fatty Acids</term><term>Phospholipids</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>DNA, Bacterial</term><term>RNA, Ribosomal, 16S</term></keywords><keywords scheme="MESH" qualifier="classification" xml:lang="en"><term>Gammaproteobacteria</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Gammaproteobacteria</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Gammaproteobacteria</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Nitrogen</term><term>Sulfur</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Bacterial Typing Techniques</term><term>Base Composition</term><term>Massachusetts</term><term>Nitrogen Fixation</term><term>Phylogeny</term><term>Sequence Analysis, DNA</term><term>Water Microbiology</term><term>Wetlands</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">A novel non-phototrophic, marine, sulfur-oxidizing bacterium, strain S-1T, was isolated from a coastal salt marsh in Massachusetts, USA. Cells are Gram-stain-negative vibrios motile by means of a single polar unsheathed flagellum. S-1T is an obligate microaerophile with limited metabolic capacity. It grows chemolithoautotrophically utilizing sulfide and thiosulfate as electron donors, converting these compounds to sulfate, and the Calvin-Benson-Bassham cycle for carbon fixation. Cells of S-1T did not grow on any of a large number of organic carbon sources and there was no evidence for chemoorganoheterotrophic growth. Cells produced internal sulfur globules during growth on sulfide and thiosulfate. S-1T is strongly diazotrophic, as demonstrated by 15N2 fixation and acetylene reduction activity by cells when a fixed nitrogen source is absent from the growth medium. The marine nature of this organism is evident from its ability to grow in 10 to 100 % artificial seawater but not at lower concentrations and NaCl alone cannot substitute for sea salts. The major cellular fatty acids are C16 : 1ω7c, C16 : 0, and C18 : 1ω7c. Phosphatidylethanolamine and phosphatidylglycerol are the major polar lipids. Q8 is the only respiratory quinone. S-1T genomic DNA has a G+C content of 67.6 mol%. Based on its 16S rRNA gene sequence, S-1T shows the closest phylogenetic relationship to non-phototrophic species within the family Thioalkalispiraceae of the class Gammaproteobacteria. The name Endothiovibrio diazotrophicus is proposed for this organism, with S-1T as the type strain (ATCC BAA-1439T=JCM 17961T).</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">27983471</PMID><DateCreated><Year>2016</Year><Month>12</Month><Day>16</Day></DateCreated><DateCompleted><Year>2017</Year><Month>08</Month><Day>25</Day></DateCompleted><DateRevised><Year>2017</Year><Month>08</Month><Day>25</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1466-5034</ISSN><JournalIssue CitedMedium="Internet"><Volume>67</Volume><Issue>5</Issue><PubDate><Year>2017</Year><Month>May</Month></PubDate></JournalIssue><Title>International journal of systematic and evolutionary microbiology</Title><ISOAbbreviation>Int. J. Syst. Evol. Microbiol.</ISOAbbreviation></Journal><ArticleTitle>Endothiovibrio diazotrophicus gen. nov., sp. nov., a novel nitrogen-fixing, sulfur-oxidizing gammaproteobacterium isolated from a salt marsh.</ArticleTitle><Pagination><MedlinePgn>1491-1498</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1099/ijsem.0.001743</ELocationID><Abstract><AbstractText>A novel non-phototrophic, marine, sulfur-oxidizing bacterium, strain S-1T, was isolated from a coastal salt marsh in Massachusetts, USA. Cells are Gram-stain-negative vibrios motile by means of a single polar unsheathed flagellum. S-1T is an obligate microaerophile with limited metabolic capacity. It grows chemolithoautotrophically utilizing sulfide and thiosulfate as electron donors, converting these compounds to sulfate, and the Calvin-Benson-Bassham cycle for carbon fixation. Cells of S-1T did not grow on any of a large number of organic carbon sources and there was no evidence for chemoorganoheterotrophic growth. Cells produced internal sulfur globules during growth on sulfide and thiosulfate. S-1T is strongly diazotrophic, as demonstrated by 15N2 fixation and acetylene reduction activity by cells when a fixed nitrogen source is absent from the growth medium. The marine nature of this organism is evident from its ability to grow in 10 to 100 % artificial seawater but not at lower concentrations and NaCl alone cannot substitute for sea salts. The major cellular fatty acids are C16 : 1ω7c, C16 : 0, and C18 : 1ω7c. Phosphatidylethanolamine and phosphatidylglycerol are the major polar lipids. Q8 is the only respiratory quinone. S-1T genomic DNA has a G+C content of 67.6 mol%. Based on its 16S rRNA gene sequence, S-1T shows the closest phylogenetic relationship to non-phototrophic species within the family Thioalkalispiraceae of the class Gammaproteobacteria. The name Endothiovibrio diazotrophicus is proposed for this organism, with S-1T as the type strain (ATCC BAA-1439T=JCM 17961T).</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Bazylinski</LastName><ForeName>Dennis A</ForeName><Initials>DA</Initials><AffiliationInfo><Affiliation>1School of Life Sciences, University of Nevada at Las Vegas, Las Vegas, Nevada 89154-4004, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Morillo</LastName><ForeName>Viviana</ForeName><Initials>V</Initials><AffiliationInfo><Affiliation>1School of Life Sciences, University of Nevada at Las Vegas, Las Vegas, Nevada 89154-4004, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Lefèvre</LastName><ForeName>Christopher T</ForeName><Initials>CT</Initials><AffiliationInfo><Affiliation>2CEA Cadarache/CNRS/Université Aix-Marseille, UMR7265 Biosciences and Biotechnologies Institute, Laboratoire de Bioénergétique Cellulaire, Saint Paul lez Durance 13108, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Viloria</LastName><ForeName>Nathan</ForeName><Initials>N</Initials><AffiliationInfo><Affiliation>1School of Life Sciences, University of Nevada at Las Vegas, Las Vegas, Nevada 89154-4004, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Dubbels</LastName><ForeName>Bradley L</ForeName><Initials>BL</Initials><AffiliationInfo><Affiliation>3Novozymes North America Inc., 9000 Development Drive, Morrisville, North Carolina 27560, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Williams</LastName><ForeName>Timothy J</ForeName><Initials>TJ</Initials><AffiliationInfo><Affiliation>4School of Biotechnology and Biomolecular Sciences, The University of New South Wales, Sydney NSW 2052, Australia.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2017</Year><Month>05</Month><Day>24</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Int J Syst Evol Microbiol</MedlineTA><NlmUniqueID>100899600</NlmUniqueID><ISSNLinking>1466-5026</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D004269">DNA, Bacterial</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D005227">Fatty Acids</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010743">Phospholipids</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D012336">RNA, Ribosomal, 16S</NameOfSubstance></Chemical><Chemical><RegistryNumber>70FD1KFU70</RegistryNumber><NameOfSubstance 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MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008404" MajorTopicYN="N">Massachusetts</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D009584" MajorTopicYN="N">Nitrogen</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D009586" MajorTopicYN="Y">Nitrogen Fixation</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010743" MajorTopicYN="N">Phospholipids</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010802" MajorTopicYN="Y">Phylogeny</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D012336" MajorTopicYN="N">RNA, Ribosomal, 16S</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D017422" MajorTopicYN="N">Sequence Analysis, DNA</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D013455" MajorTopicYN="N">Sulfur</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014871" MajorTopicYN="Y">Water Microbiology</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D053833" MajorTopicYN="Y">Wetlands</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2016</Year><Month>12</Month><Day>17</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2017</Year><Month>8</Month><Day>26</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2016</Year><Month>12</Month><Day>17</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId 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